Shiva Hosseini | 1 Article |
<b>Objectives</b><br/>
Widespread use of β-lactam antibiotics could cause resistance to this group of antibiotics in pathogenic bacteria through the production of the enzyme β-lactamases. The aim of this study is to determine the molecular detection of AmpC β-lactamases among clinical <i>Escherichia coli</i> isolated from Ilam hospitals in Ilam, Iran.<br/><b>Methods</b><br/>
One hundred and twelve clinical isolates of <i>E. coli</i> were collected from hospitalized patients and were identified by biochemical tests. They were evaluated for extended spectrum beta-lactamases (ESBLs) production, and the positive strains were subjected to AmpC enzymes; for detection of AmpC cluster genes, multiplex polymerase chain reaction was applied.<br/><b>Results</b><br/>
The analysis showed 62.5% of isolates were ESBLs positive and that five strains revealed the AmpC cluster genes. This is the first report of <i>FOXM</i> cluster genes in <i>E. coli</i> in Iran.<br/><b>Conclusion</b><br/>
Based on our results, the prevalence of AmpC β-lactamases is increasing in Iran, which caused failure in antibiotic therapy. So, the current study recommended the revision of antibiotic policy in Iranian hospitals.
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