Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives

OPEN ACCESS
SEARCH
Search

Author index

Page Path
HOME > Articles and issues > Author index
Search
Shanta Dutta 1 Article
Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon
Wiliane J. T. Marbou, Priyanka Jain, Sriparna Samajpati, Gourab Halder, Asish K. Mukhopadhyay, Shanta Dutta, Victor Kuete
Osong Public Health Res Perspect. 2020;11(4):216-230.   Published online August 31, 2020
DOI: https://doi.org/10.24171/j.phrp.2020.11.4.11
  • 8,839 View
  • 153 Download
  • 6 Web of Science
  • 7 Crossref
AbstractAbstract PDF
Objectives

This study aimed to identify virulent and antimicrobial resistant genes in fecal E. coli in Mbouda, Cameroon.

Methods

A total of 599 fecal samples were collected from patients with enteric infections who were ≥ 20 years old. E. coli was isolated on the MacConkey agar and virulent genes were detected by multiplex/simplex PCR. Isolates in which ≥ 1 virulent gene was detected were subjected to antibiotic susceptibility testing. The resulting resistant isolates were subjected to PCR, followed by sequencing for resistant genes detection.

Results

There were 119 enterovirulent E. coli identified, amongst which 47.05% were atypical enteropathogenic E. coli (EPEC), 36.97% enterotoxigenic E. coli, 10.08% Shiga toxin producing E. coli (STEC) and 5.88% were enteroinvasive E. coli (EIEC). The occurrence of the eae gene (47.06%) was higher compared with CVD432 (33.61%), aaic (13.45%), stx2 (10.08%) and stx1 (0.84%). High resistance rates were noted for ampicillin (94.64% EPEC, 91.67% STEC, 59.09% EAEC, and 57.14% EIEC) and sulfamethoxazole-trimethoprim (100% EPEC and 83.33% STEC, 81.82% EAEC and 71.43% EIEC). sul2 (71.43%), tetB (64.71%), tetA (59.94%) and blaTEM (52.10%) were detected. A double mutation (S83L; D87N) was seen in gyrA and a single mutation (S80I) was observed in parC.

Conclusion

These findings suggested that measures should be taken to reduce the harm of E. coli to public health.

Citations

Citations to this article as recorded by  
  • Multidrug Resistant Enteric Bacteria from Cancer Patients Admitted in Douala Laquintinie Hospital, Littoral Region of Cameroon
    Michael F. Kengne, Ornella D. Tsobeng, Ballue S. T. Dadjo, Victor Kuete, Armelle T. Mbaveng, Tingtao Chen
    Canadian Journal of Infectious Diseases and Medica.2024;[Epub]     CrossRef
  • Molecular Epidemiology and AMR Perspective of Diarrhoeagenic Escherichia coli in Africa: A Systematic Review and Meta-analysis
    John Bosco Kalule, Linda A. Bester, Daniel L. Banda, Firehiwot Abera Derra, Chisomo Msefula, Anthony M. Smith, Abraham Ajayi, Happiness Kumburu, Geoffrey Kwenda, Kaunda Yamba, John Mwaba, Yasmina J. Fakim, Nyasha Sithole, Aquillah M. Kanzi, Patrick M. K.
    Journal of Epidemiology and Global Health.2024;[Epub]     CrossRef
  • Molecular epidemiology of antimicrobial resistance in central africa: A systematic review
    Annicet-Clotaire Dikoumba, Richard Onanga, Laurette G. Mangouka, Larson Boundenga, Edgard-Brice Ngoungou, Sylvain Godreuil
    Access Microbiology .2023;[Epub]     CrossRef
  • Methanol extract from the seeds of Persea americana displays antibacterial and wound healing activities in rat model
    Steve E. Ekom, Jean-De-Dieu Tamokou, Victor Kuete
    Journal of Ethnopharmacology.2022; 282: 114573.     CrossRef
  • Characterization of diarrhoeagenic Escherichia coli with special reference to antimicrobial resistance isolated from hospitalized diarrhoeal patients in Kolkata (2012–2019), India
    Debjani Ghosh, Goutam Chowdhury, Prosenjit Samanta, Sreeja Shaw, Alok K. Deb, Mainak Bardhan, Asis Manna, Shin-ichi Miyoshi, Thandavarayan Ramamurthy, Shanta Dutta, Asish K. Mukhopadhyay
    Journal of Applied Microbiology.2022; 132(6): 4544.     CrossRef
  • Antibiotic resistomes and their chemical residues in aquatic environments in Africa
    Aemere Ogunlaja, Olumuyiwa O. Ogunlaja, Olumide D. Olukanni, Gloria O. Taylor, Chidinma G. Olorunnisola, Victorien T. Dougnon, Wassiyath Mousse, Despo Fatta-Kassinos, Titus A.M. Msagati, Emmanuel I. Unuabonah
    Environmental Pollution.2022; 312: 119783.     CrossRef
  • Antibacterial and Therapeutic Potentials of the Capsicum annuum Extract against Infected Wound in a Rat Model with Its Mechanisms of Antibacterial Action
    Steve Endeguele Ekom, Jean-De-Dieu Tamokou, Victor Kuete, Dorota Formanowicz
    BioMed Research International.2021; 2021: 1.     CrossRef

PHRP : Osong Public Health and Research Perspectives
TOP