- Multilocus Sequence Analysis of Housekeeping Genes and Antigenic Determinant Genes in Bordetella pertussis Strains Isolated in Korea
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Sang-Oun Jung, Yu Mi Moon, So-Hyeon Kim, Hwa Young Sung, Seung-Jik Kwon, Yeon Ho Kang, Jae Yon Yu
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Osong Public Health Res Perspect. 2011;2(2):115-126. Published online June 30, 2011
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DOI: https://doi.org/10.1016/j.phrp.2011.08.003
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- Objectives
To confirm genotype diversities of clinical isolates of Bordetella pertussis and to evaluate the risk of pertussis outbreak in Korea.
Methods
Seven housekeeping genes and 10 antigenic determinant genes from clinical B. pertussis isolates were analyzed by Multilocus sequence typing (MLST).
Results
More variant pattern was observed in antigenic determinant genes. Especially, PtxS1 gene was the most variant gene; five genotypes were observed from eight global genotypes. In the bacterial type, the number of observed sequence types in the isolates was seven and the most frequent form was type 1 (79.6%). This major sequence type also showed a time-dependent transition pattern. Older isolates (1968 and 1975) showed type 1 and 6 in housekeeping genes and antigenic determinant genes, respectively. However, these were changed to type 2 and 1 in isolates 1999–2008. This transition was mainly attributed to genotype change of PtxS1 and Fim3 gene; the tendency of genotype change was to avoid vaccine-derived genotype. In addition, there was second transition in 2009. In this period, only the sequence type of antigenic determinant genes was changed to type 2. Based Upon Related Sequence Types (BURST) analysis confirmed that there were two clonal complexes (ACCI and ACCII) in the Korean isolates. Moreover, the recently increased sequence type was revealed as AST2 derived from AST 3 in ACCI.
Conclusions
Genotype changes in Korean distributing strains are still progressing and there was a specific driving force in antigenic determinant genes. Therefore continuous surveillance of genotype change of the distributing strains should be performed to confirm interrelationship of genotype change with vaccine immunity.
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Citations
Citations to this article as recorded by  - Characterization of Bordetella pertussis Strains Isolated from India
Shweta Alai, Manish Gautam, Sonali Palkar, Jitendra Oswal, Sunil Gairola, Dhiraj P. Dhotre
Pathogens.2022; 11(7): 794. CrossRef - Variation in Bordetella pertussis Susceptibility to Erythromycin and Virulence-Related Genotype Changes in China (1970-2014)
Ying Yang, Kaihu Yao, Xiang Ma, Wei Shi, Lin Yuan, Yonghong Yang, Daniela Flavia Hozbor
PLOS ONE.2015; 10(9): e0138941. CrossRef - Recent Trends of Antigenic Variation inBordetella pertussisIsolates in Korea
So-Hyun Kim, Jin Lee, Hwa Young Sung, Jae Yon Yu, Seong Han Kim, Mi Sun Park, Sang-Oun Jung
Journal of Korean Medical Science.2014; 29(3): 328. CrossRef
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