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Seul-Ki Park 2 Articles
Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
Osong Public Health Res Perspect. 2016;7(6):351-355.   Published online December 31, 2016
DOI: https://doi.org/10.1016/j.phrp.2016.11.004
  • 3,614 View
  • 23 Download
  • 2 Crossref
AbstractAbstract PDF
Objectives
Progastrin-releasing peptide (proGRP) is a promising biomarker for small cell lung cancer. However, not much is known about how sample processing and storage conditions affect the stability of proGRP. Here, we examined the effects of repeated freeze–thaw cycles on the stability of proGRP in plasma and serum.
Methods
Concentrations of proGRP were measured in plasma and serum samples exposed to two, three, or four freeze–thaw cycles and these were compared with values of corresponding samples exposed to one cycle (baseline). We also performed the area under the receiver-operating-characteristic curve (AUC) analysis to determine whether the differences of proGRP concentrations between each paired plasma and serum sample (ΔproGRP) can be used for identifying the samples that have been exposed to multiple freeze–thaw cycles.
Results
Concentrations of proGRP gradually decreased in both plasma and serum samples with increasing numbers of freeze–thaw cycles. Reduction rates of proGRP concentrations were greater in serum than in plasma samples and serum proGRP levels declined with statistical significance (p < 0.001) up to 10.1% after four freeze–thaw cycles. The ΔproGRP measurement showed fair accuracy (AUC = 0.741) for identifying samples that had been through four freeze–thaw cycles. The sensitivity was 82.8% and specificity was 62.1% at an optimal cut-off point of > 4.9.
Conclusion
Our study shows that the stability of circulating proGRP is affected in both plasma and serum samples by repeated freezing and thawing. We also show that ΔproGRP could be used for identifying paired plasma and serum samples subjected to multiple freeze–thaw cycles.

Citations

Citations to this article as recorded by  
  • Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
    Alessandro Torelli, Elena Gianchecchi, Martina Monti, Pietro Piu, Irene Barneschi, Carolina Bonifazi, Rosa Coluccio, Luisa Ganfini, Luciano Michele La Magra, Silvia Marconi, Ginevra Marzucchi, Ramona Pace, Laura Palladino, Bernardo Biagi, Emanuele Montomo
    Vaccines.2021; 9(3): 267.     CrossRef
  • The influence of different blood samples treatment methods on pro-gastrin-releasing peptide
    Huiqin Jiang, Ling Luo, Kang Xiong, Chengwen He, Huaizhou Wang, Yanghua Qin
    Medicine.2019; 98(26): e16130.     CrossRef
Inorganic Phosphorus and Potassium Are Putative Indicators of Delayed Separation of Whole Blood
Jae-Eun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, Jin-Sook Wang, Haewon Shin, Jong-Wan Kim, Bok-Ghee Han, So-Youn Shin
Osong Public Health Res Perspect. 2016;7(2):90-95.   Published online April 30, 2016
DOI: https://doi.org/10.1016/j.phrp.2015.11.003
  • 3,456 View
  • 19 Download
  • 9 Crossref
AbstractAbstract PDF
Objectives
The delayed separation of whole blood can influence the concentrations of circulating blood components, including metabolites and cytokines. The aim of this study was to determine whether clinical-biochemistry analytes can be used to assess the delayed separation of whole blood.
Methods
We investigated the plasma and serum concentrations of five clinical-biochemistry analytes and free hemoglobin when the centrifugation of whole blood stored at 4°C or room temperature was delayed for 4 hours, 6 hours, 24 hours, or 48 hours, and compared the values with those of matched samples that had been centrifuged within 2 hours after whole-blood collection.
Results
The inorganic phosphorus (IP) levels in the plasma and serum samples were elevated ≥ 1.5-fold when whole-blood centrifugation was delayed at room temperature for 48 hours. Furthermore, the IP levels in the plasma samples showed excellent assessment accuracy [area under the receiver-operating-characteristic curve (AUC) > 0.9] after a 48-hour delay in whole-blood separation, and high sensitivity (100%) and specificity (95%) at an optimal cutoff point. The IP levels in the serum samples also exhibited good assessment accuracy (AUC > 0.8), and high sensitivity (81%) and specificity (100%). The potassium (K+) levels were elevated 1.4-fold in the serum samples following a 48-hour delay in whole-blood separation. The K+ levels showed excellent assessment accuracy (AUC > 0.9) following a 48-hour delay in whole-blood separation, and high sensitivity (95%) and specificity (91%) at an optimal cutoff point.
Conclusion
Our study showed that the IP and K+ levels in the plasma or serum samples could be considered as putative indicators to determine whether whole-blood separation had been delayed for extended periods.

Citations

Citations to this article as recorded by  
  • Assessing the stability of uncentrifuged serum and plasma analytes at various post-collection intervals
    Atiqah Mokhsin, Poonaresi Subramaniam, Sivasooriar Sivaneson, Nelson Nheu, Gobhy Ramaloo, Azana S. Hanifah, Sumitha B. Mahathevan, Mohanaraja Nadarajah, Gayathiri Sampasivam, Aletza Mohd Ismail, Thuhairah Abdul Rahman
    Journal of Laboratory Medicine.2024;[Epub]     CrossRef
  • Concordance in COVID-19 serology, bone mineralization, and inflammatory analytes between venous and self-collected capillary blood samples exposed to various pre-analytical conditions
    Banafshe Hosseini, Harika Dasari, Anna Smyrnova, Claude Bourassa, Jing Leng, Christian Renaud, Francine M Ducharme
    Annals of Clinical Biochemistry: International Jou.2023; 60(4): 259.     CrossRef
  • Assessment of avian health status: suitability and constraints of the Zoetis VetScan VS2 blood analyser for ecological and evolutionary studies
    Ye Xiong, Michael Tobler, Arne Hegemann, Dennis L. Hasselquist
    Biology Open.2023;[Epub]     CrossRef
  • Blood Plasma Quality Control by Plasma Glutathione Status
    Tamara Tomin, Natalie Bordag, Elmar Zügner, Abdullah Al-Baghdadi, Maximilian Schinagl, Ruth Birner-Gruenberger, Matthias Schittmayer
    Antioxidants.2021; 10(6): 864.     CrossRef
  • Identification of specific pre-analytical quality control markers in plasma and serum samples
    Luz Ruiz-Godoy, Virginia Enríquez-Cárcamo, Lourdes Suárez-Roa, María Lourdes Lopez-Castro, Abel Santamaría, Mario Orozco-Morales, Ana Laura Colín-González
    Analytical Methods.2019; 11(17): 2259.     CrossRef
  • Proteomics and Lipidomics in Inflammatory Bowel Disease Research: From Mechanistic Insights to Biomarker Identification
    Bjoern Titz, Raffaella M. Gadaleta, Giuseppe Lo Sasso, Ashraf Elamin, Kim Ekroos, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng
    International Journal of Molecular Sciences.2018; 19(9): 2775.     CrossRef
  • Impact of Preanalytical Variations in Blood-Derived Biospecimens on Omics Studies: Toward Precision Biobanking?
    Jae-Eun Lee, Young-Youl Kim
    OMICS: A Journal of Integrative Biology.2017; 21(9): 499.     CrossRef
  • Effect of delayed centrifugation of whole blood on serum samples stability
    Massimo Daves, Vincenzo Roccaforte, Michele Giacomi, Monica Riva, Maria Leitner, Stefan Platzgummer, Gertraud Goetsch, Giuseppe Lippi
    La Rivista Italiana della Medicina di Laboratorio .2017; 13(1): 41.     CrossRef
  • Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
    Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
    Osong Public Health and Research Perspectives.2016; 7(6): 351.     CrossRef

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