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Seonghan Kim 4 Articles
Molecular Typing of Mycobacterium intracellulare Using Pulsed-Field Gel Electrophoresis, Variable-Number Tandem-Repeat Analysis, Mycobacteria Interspersed Repetitive-Unit-Variable-Number Tandem Repeat Typing, and Multilocus Sequence Typing: Molecular Characterization and Comparison of Each Typing Methods
Semi Jeon, Nara Lim, Seungjik Kwon, Taesun Shim, Misun Park, Bum-Joon Kim, Seonghan Kim
Osong Public Health Res Perspect. 2014;5(3):119-130.   Published online June 30, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.04.003
  • 1,894 View
  • 14 Download
  • 8 Citations
AbstractAbstract PDF
Objectives
Mycobacterium intracellulare is the major causative agent of nontuberculous mycobacteria-related pulmonary infections. The strain typing of M. intracellulare is important for the treatment and control of its infections. We compared the discrimination capacity and effective value of four different molecular typing methods.
Methods
Antibiotic susceptibility testing, hsp65 and rpoB sequencing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), mycobacteria interspersed repetitive-unit-variable-number tandem-repeat analysis (MIRU-VNTR), and VNTR assay targeting 44 M. intracellulare isolates obtained from patients with pulmonary infections were performed.
Results
All the antibiotic susceptibility patterns had no association with the molecular and sequence types tested in this study; however, the molecular and sequence types were related with each other. PFGE gave best results for discriminatory capacity, followed by VNTR, MLST, and MIRU-VNTR.
Conclusion
The high discriminatory power of PFGE, VNTR, and MLST is enough for differentiating between reinfection and relapse, as well as for other molecular epidemiological usages. The MLST could be regarded as a representative classification method, because it showed the clearest relation with the sequence types.

Citations

Citations to this article as recorded by  
  • Differential Genotyping of Mycobacterium avium Complex and Its Implications in Clinical and Environmental Epidemiology
    Jeong-Ih Shin, Sung Jae Shin, Min-Kyoung Shin
    Microorganisms.2020; 8(1): 98.     CrossRef
  • A strategy based on Amplified Fragment Length Polymorphism (AFLP) for routine genotyping of nontuberculous mycobacteria at the clinical laboratory
    Sara Blanco-Conde, Carolina González-Cortés, Ramiro López-Medrano, Juan José Palacios-Gutiérrez, Cristina Diez-Tascón, Teresa Nebreda-Mayoral, María Josefa Sierra-García, Octavio Miguel Rivero-Lezcano
    Molecular Biology Reports.2020; 47(5): 3397.     CrossRef
  • Comparative Evaluation of Band-Based Genotyping Methods for Mycobacterium intracellulare and Its Application for Epidemiological Analysis
    Jeong-Ih Shin, Jong-Hun Ha, Dong-Hae Lee, Jeong-Gyu Choi, Kyu-Min Kim, Seung Jun Lee, Yi Yeong Jeong, Jong Deog Lee, Myunghwan Jung, Seung-Chul Baik, Woo Kon Lee, Hyung-Lyun Kang, Min-Kyoung Shin, Jung-Wan Yoo
    Microorganisms.2020; 8(9): 1315.     CrossRef
  • Pulsed Field Gel Electrophoresis: Past, present, and future
    Lilia Lopez-Canovas, Maximo B. Martinez Benitez, Jose A. Herrera Isidron, Eduardo Flores Soto
    Analytical Biochemistry.2019; 573: 17.     CrossRef
  • Molecular typing of Mycobacterium kansasii using pulsed-field gel electrophoresis and a newly designed variable-number tandem repeat analysis
    Zofia Bakuła, Anna Brzostek, Paulina Borówka, Anna Żaczek, Izabela Szulc-Kiełbik, Agata Podpora, Paweł Parniewski, Dominik Strapagiel, Jarosław Dziadek, Małgorzata Proboszcz, Jacek Bielecki, Jakko van Ingen, Tomasz Jagielski
    Scientific Reports.2018;[Epub]     CrossRef
  • Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare
    So-Young Lee, Byoung-Jun Kim, Hong Kim, Yu-Seop Won, Che Ok Jeon, Joseph Jeong, Seon Ho Lee, Ji-Hun Lim, Seung-Heon Lee, Chang Ki Kim, Yoon-Hoh Kook, Bum-Joon Kim
    International Journal of Systematic and Evolution.2016; 66(8): 3132.     CrossRef
  • Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria
    Tomasz Jagielski, Alina Minias, Jakko van Ingen, Nalin Rastogi, Anna Brzostek, Anna Żaczek, Jarosław Dziadek
    Clinical Microbiology Reviews.2016; 29(2): 239.     CrossRef
  • Genetic diversity of clinical Mycobacterium avium subsp. hominissuis and Mycobacterium intracellulare isolates causing pulmonary diseases recovered from different geographical regions
    Kazuya Ichikawa, Jakko van Ingen, Won-Jung Koh, Dirk Wagner, Max Salfinger, Takayuki Inagaki, Kei-ichi Uchiya, Taku Nakagawa, Kenji Ogawa, Kiyofumi Yamada, Tetsuya Yagi
    Infection, Genetics and Evolution.2015; 36: 250.     CrossRef
Multiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus
Jie Yeun Park, Semi Jeon, Jun Young Kim, Misun Park, Seonghan Kim
Osong Public Health Res Perspect. 2013;4(3):133-139.   Published online June 30, 2013
DOI: https://doi.org/10.1016/j.phrp.2013.04.004
  • 2,146 View
  • 22 Download
  • 15 Citations
AbstractAbstract PDF
Objectives
A multiplex real-time polymerase chain reaction (RT-PCR) method was developed for the identification of three Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus.
Methods
Specific primers and probes targeting the hlyA, tlh, and vvhA genes were selected and used for multiplex real-time PCR to confirm the identification of V. cholerae, V. parahaemolyticus, and V. vulnificus, respectively. This method was applied to screen Vibrio species from environmental samples and combining it with a culture-based method, its effectiveness was evaluated in comparison with culture-based methods alone.
Results
Specific PCR fragments were obtained from isolates belonging to the target species, indicating a high specificity of this multiplex real-time PCR. No cross-reactivity with the assay was observed between the tested bacteria. The sensitivity of the multiplex real-time PCR was found to have a lower limit of 104 colony-forming units/reaction for all three Vibrio species. The combination strategy raised the isolation ratio of all three Vibrio species 1.26- to 2.75-fold.
Conclusion
This assay provides a rapid, sensitive, and specific technique to detect these three Vibrio species in the environment.

Citations

Citations to this article as recorded by  
  • Current trends in polymerase chain reaction based detection of three major human pathogenic vibrios
    Sharmin Quazi Bonny, M. A. Motalib Hossain, Syed Muhammad Kamal Uddin, Thiruchelvi Pulingam, Suresh Sagadevan, Mohd Rafie Johan
    Critical Reviews in Food Science and Nutrition.2022; 62(5): 1317.     CrossRef
  • Highly lethal Vibrio parahaemolyticus strains cause acute mortality in Penaeus vannamei post-larvae
    Feng Yang, Limei Xu, Wanzhen Huang, Fang Li
    Aquaculture.2022; 548: 737605.     CrossRef
  • Hepatopancreatic transcriptome analysis and humoral immune factor assays in red claw crayfish (Cherax quadricarinatus) provide insight into innate immunomodulation under Vibrio parahaemolyticus infection
    Duanduan Chen, Leifeng Guo, Cao Yi, Shouquan Wang, Yuanyuan Ru, Hui Wang
    Ecotoxicology and Environmental Safety.2021; 217: 112266.     CrossRef
  • Molecular mechanisms of Vibrio parahaemolyticus pathogenesis
    Lingzhi Li, Hongmei Meng, Dan Gu, Yang Li, Mengdie Jia
    Microbiological Research.2019; 222: 43.     CrossRef
  • Application of digital PCR and next generation sequencing in the etiology investigation of a foodborne disease outbreak caused by Vibrio parahaemolyticus
    Ying Li, Shuang Zhang, Jie Li, Meiling Chen, Mu He, Yuanyuan Wang, Yanchun Zhang, Hongbo Jing, Hongmei Ma, Yindong Li, Lin Zhao, Hongqun Zhao, Biao Kan, Bo Pang
    Food Microbiology.2019; 84: 103233.     CrossRef
  • Cholera Outbreak due to Raw Seafood Consumption in South Korea, 2016
    Jeong Hyun Kim, Jin Lee, Sahyun Hong, Sangwon Lee, Hae-young Na, Young-Il Jeong, Eun Jin Choi, Junyoung Kim, Hyo Sun Kawk, Enhi Cho
    The American Journal of Tropical Medicine and Hygi.2018; 99(1): 168.     CrossRef
  • Detection and differentiation of Vibrio parahaemolyticus by multiplexed real-time PCR
    Deshun Xu, Lei Ji, Xiaofang Wu, Wei Yan, Liping Chen
    Canadian Journal of Microbiology.2018; 64(11): 809.     CrossRef
  • Development of a rapid PCR protocol to detect Vibrio parahaemolyticus in clams
    Sara Federici, Diana I. Serrazanetti, M. Elisabetta Guerzoni, Raffaella Campana, Eleonora Ciandrini, Wally Baffone, Andrea Gianotti
    Journal of Food Science and Technology.2018; 55(2): 749.     CrossRef
  • First Case of Necrotizing Fasciitis Caused by Skermanella aerolata Infection Mimicking Vibrio Sepsis
    Sang Taek Heo, Ki Tae Kwon, Jeong Rae Yoo, Ji Young Choi, Keun Hwa Lee, Kwan Soo Ko
    Annals of Laboratory Medicine.2018; 38(6): 604.     CrossRef
  • Vibrio cholerae O1 with Reduced Susceptibility to Ciprofloxacin and Azithromycin Isolated from a Rural Coastal Area of Bangladesh
    Shah M. Rashed, Nur A. Hasan, Munirul Alam, Abdus Sadique, Marzia Sultana, Md. Mozammel Hoq, R. Bradley Sack, Rita R. Colwell, Anwar Huq
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • A label-free multi-functionalized graphene oxide based electrochemiluminscence immunosensor for ultrasensitive and rapid detection of Vibrio parahaemolyticus in seawater and seafood
    Yuhong Sha, Xuan Zhang, Wenrou Li, Wei Wu, Sui Wang, Zhiyong Guo, Jun Zhou, Xiurong Su
    Talanta.2016; 147: 220.     CrossRef
  • Toxigenic Vibrio cholerae O1 in vegetables and fish raised in wastewater irrigated fields and stabilization ponds during a non-cholera outbreak period in Morogoro, Tanzania: an environmental health study
    Yaovi M. G. Hounmanou, Robinson H. Mdegela, Tamègnon V. Dougnon, Ofred J. Mhongole, Edward S. Mayila, Joseph Malakalinga, George Makingi, Anders Dalsgaard
    BMC Research Notes.2016;[Epub]     CrossRef
  • Isolation of Vibrio vulnificus Biotype I from Disease Outbreaks on Cultured Tiger Grouper Epinephelus fuscoguttatus Forsskal, 1775
    Jumroensri Thawonsuwan, Jiraporn Kasornchandra, Patcharee Soonsan, Chantana Keawtapee
    Fish Pathology.2016; 51(Special-is): S39.     CrossRef
  • Detection of Vibrio cholerae by isothermal cross-priming amplification combined with nucleic acid detection strip analysis
    Xia Zhang, Xin-Jun Du, Chun Guan, Ping Li, Wen-Jie Zheng, Shuo Wang
    Molecular and Cellular Probes.2015; 29(4): 208.     CrossRef
  • lolB gene, a valid alternative for qPCR detection of Vibrio cholerae in food and environmental samples
    Alejandro Garrido-Maestu, María-José Chapela, Juan M. Vieites, Ana G. Cabado
    Food Microbiology.2015; 46: 535.     CrossRef
Availability of Clean Tap Water and Medical Services Prevents the Incidence of Typhoid Fever
Deog-Yong Lee, Esther Lee, HyeMin Park, SeongHan Kim
Osong Public Health Res Perspect. 2013;4(2):68-71.   Published online April 30, 2013
DOI: https://doi.org/10.1016/j.phrp.2013.03.005
  • 2,087 View
  • 19 Download
  • 2 Citations
AbstractAbstract PDF
Objective: In this study, the factors that induced a decrease in the incidence of typhoid fever were analyzed. Based on the study results, we propose a quantitative and concrete solution to reduce the incidence of typhoid fever.
Methods
We analyzed the incidence and fatality rate of typhoid fever in Korea. Tap water service rate and the number of pharmacies, which affect the incidence rate of typhoid fever, were used as environmental factors.
Results
To prevent typhoid fever in the community, it is necessary to provide clean tap water service to 35.5% of the population, with an individual requiring 173 L of clean water daily. Appropriate access to clean water (51% service coverage, 307 L) helped the population to maintain individual hygiene and food safety practices, which brought about a decrease in the incidence of typhoid fever, and subsequently a decrease in fatality rate, which was achieved twice. During the 8-year study period, the fatality rate decreased to 1% when the population has access to proper medical service.
Conclusion
The fatality rate was primarily affected by the availability of medical services as well as by the incidence of typhoid fever. However, an analysis of the study results showed that the incidence of typhoid fever was affected only by the availability of clean water through the tap water system.

Citations

Citations to this article as recorded by  
  • TIPICO X: report of the 10th interactive infectious disease workshop on infectious diseases and vaccines
    Irene Rivero-Calle, Jose Gómez-Rial, Louis Bont, Bradford D. Gessner, Melvin Kohn, Ron Dagan, Daniel C. Payne, Laia Bruni, Andrew J. Pollard, Adolfo García-Sastre, Denise L. Faustman, Albert Osterhaus, Robb Butler, Francisco Giménez Sánchez, Francisco Álv
    Human Vaccines & Immunotherapeutics.2021; 17(3): 759.     CrossRef
  • Progress in the overall understanding of typhoid fever: implications for vaccine development
    Peter J O’Reilly, Dikshya Pant, Mila Shakya, Buddha Basnyat, Andrew J Pollard
    Expert Review of Vaccines.2020; 19(4): 367.     CrossRef
Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of gyrA and parC Mutations in Quinolone-Resistant Escherichia coli and Shigella spp.
Junyoung Kim, Semi Jeon, Hyungjun Kim, Misun Park, Soobok Kim, Seonghan Kim
Osong Public Health Res Perspect. 2012;3(2):113-117.   Published online June 30, 2012
DOI: https://doi.org/10.1016/j.phrp.2012.04.004
  • 2,043 View
  • 14 Download
  • 5 Citations
AbstractAbstract PDF
Two real-time polymerase chain reaction assays were developed to detect mutations in codons 83 and 87 in gyrA and in codons 80 and 91 in parC, the main sites that causes quinolone resistance in pathogenic Escherichia coli and Shigella spp. isolates. These assays can be employed as a useful method for controlling infections caused by quinolone-resistant E coli and Shigella isolates.

Citations

Citations to this article as recorded by  
  • Molecular detection and Frequency of Fluoroquinolone-Resistant Escherichia coli by Multiplex Allele Specific Polymerase Chain Reaction (MAS-PCR)
    Noha Tharwat Abou El-Khier, Maysaa El Sayed Zaki
    Egyptian Journal of Basic and Applied Sciences.2020; 7(1): 1.     CrossRef
  • Identification of new DNA gyrase inhibitors based on bioactive compounds from streptomyces: structure-based virtual screening and molecular dynamics simulations approaches
    Hourieh Kalhor, Solmaz Sadeghi, Mahya Marashiyan, Reyhaneh Kalhor, Sanaz Aghaei Gharehbolagh, Mohammad Reza Akbari Eidgahi, Hamzeh Rahimi
    Journal of Biomolecular Structure and Dynamics.2020; 38(3): 791.     CrossRef
  • Soft sweep development of resistance in Escherichia coli under fluoroquinolone stress
    Xianxing Xie, Ruichen Lv, Chao Yang, Yajun Song, Yanfeng Yan, Yujun Cui, Ruifu Yang
    Journal of Microbiology.2019; 57(12): 1056.     CrossRef
  • Rapid Detection of Genomic Mutations ingyrAandparCGenes ofEscherichia coliby Multiplex Allele Specific Polymerase Chain Reaction
    Sukanlayanee Onseedaeng, Panan Ratthawongjirakul
    Journal of Clinical Laboratory Analysis.2016; 30(6): 947.     CrossRef
  • New concepts in diagnostics for infectious diarrhea
    J A Platts-Mills, J Liu, E R Houpt
    Mucosal Immunology.2013; 6(5): 876.     CrossRef

PHRP : Osong Public Health and Research Perspectives