- Corrigendum to “Traditional and Modern Cell Culture in Virus Diagnosis”[Osong Public Health Res Perspect 2016;7(2):77–82]
-
Ali Hematian, Nourkhoda Sadeghifard, Reza Mohebi, Morovat Taherikalani, Abbas Nasrolahi, Mansour Amraei, Sobhan Ghafourian
-
Osong Public Health Res Perspect. 2020;11(4):266-266. Published online August 31, 2020
-
DOI: https://doi.org/10.24171/j.phrp.2020.11.4.18
-
Corrects: Osong Public Health Res Perspect 2016;7(2):77
-
6,433
View
-
81
Download
-
1
Web of Science
-
1
Crossref
-
 PDF
-
Citations
Citations to this article as recorded by  - Micro and Nanoscale Technologies for Diagnosis of Viral Infections
Fatemeh Nasrollahi, Reihaneh Haghniaz, Vahid Hosseini, Elham Davoodi, Mahboobeh Mahmoodi, Solmaz Karamikamkar, Mohammad Ali Darabi, Yangzhi Zhu, Junmin Lee, Sibel Emir Diltemiz, Hossein Montazerian, Sivakoti Sangabathuni, Maryam Tavafoghi, Vadim Jucaud, W
Small.2021;[Epub] CrossRef
- Traditional and Modern Cell Culture in Virus Diagnosis
-
Ali Hematian, Nourkhoda Sadeghifard, Reza Mohebi, Morovat Taherikalani, Abbas Nasrolahi, Mansour Amraei, Sobhan Ghafourian
-
Osong Public Health Res Perspect. 2016;7(2):77-82. Published online April 30, 2016
-
DOI: https://doi.org/10.1016/j.phrp.2015.11.011
-
Correction in: Osong Public Health Res Perspect 2020;11(4):266
-
9,381
View
-
166
Download
-
72
Crossref
-
 Abstract
PDF
- Cell cultures are developed from tissue samples and then disaggregated by mechanical, chemical, and enzymatic methods to extract cells suitable for isolation of viruses. With the recent advances in technology, cell culture is considered a gold standard for virus isolation. This paper reviews the evolution of cell culture methods and demonstrates why cell culture is a preferred method for identification of viruses. In addition, the advantages and disadvantages of both traditional and modern cell culture methods for diagnosis of each type of virus are discussed. Detection of viruses by the novel cell culture methods is considered more accurate and sensitive. However, there is a need to include some more accurate methods such as molecular methods in cell culture for precise identification of viruses.
-
Citations
Citations to this article as recorded by
- Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei
-
Amir Ghasemi, Sobhan Ghafourian, Sedighe Vafaei, Reza Mohebi, Maryam Farzi, Morovat Taherikalani, Nourkhoda Sadeghifard
-
Osong Public Health Res Perspect. 2015;6(6):336-340. Published online December 31, 2015
-
DOI: https://doi.org/10.1016/j.phrp.2015.10.003
-
-
3,920
View
-
30
Download
-
3
Crossref
-
Abstract
PDF
- Objectives
In an attempt α-amylase gene from Pyrococcus woesei was amplified and cloned into a pTYB2 vector to generate the recombinant plasmid pTY- α-amylase.
Methods
Escherichia coli BL21 used as a host and protein expression was applied using IPTG. SDS-PAGE assay demonstrated the 100 kDa protein. Amylolytic activity of proteins produced by transformed E. coli cells was detected by zymography, and the rate of active α-amylase with and without the intein tag in both soluble conditions and as inclusion bodies solubilized by 4M urea were measured.
Results
Amylolytic activity of ∼185,000 U/L of bacterial culture was observed from the soluble form of the protein using this system.
Conclusion
These results indicate that this expression system was appropriate for the production of thermostable α-amylase.
-
Citations
Citations to this article as recorded by - Escherichia coli expression and characterization of α-amylase from Geobacillus thermodenitrificans DSM-465
A. Al-Amri, M. A. Al-Ghamdi, J. A. Khan, H. N. Altayeb, H. Alsulami, M. Sajjad, O. A. Baothman, M. S. Nadeem
Brazilian Journal of Biology.2022;[Epub] CrossRef - Glycoside Hydrolases and Glycosyltransferases from Hyperthermophilic Archaea: Insights on Their Characteristics and Applications in Biotechnology
Khadija Amin, Sylvain Tranchimand, Thierry Benvegnu, Ziad Abdel-Razzak, Hala Chamieh
Biomolecules.2021; 11(11): 1557. CrossRef - Optimization, Purification, and Starch Stain Wash Application of Two Newα-Amylases Extracted from Leaves and Stems ofPergularia tomentosa
Imen Lahmar, Hanen El Abed, Bassem Khemakhem, Hafedh Belghith, Ferjani Ben Abdallah, Karima Belghith
BioMed Research International.2017; 2017: 1. CrossRef
|
