Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives

OPEN ACCESS
SEARCH
Search

Author index

Page Path
HOME > Articles and issues > Author index
Search
Reza Mohebi 3 Articles
Corrigendum to “Traditional and Modern Cell Culture in Virus Diagnosis”[Osong Public Health Res Perspect 2016;7(2):77–82]
Ali Hematian, Nourkhoda Sadeghifard, Reza Mohebi, Morovat Taherikalani, Abbas Nasrolahi, Mansour Amraei, Sobhan Ghafourian
Osong Public Health Res Perspect. 2020;11(4):266-266.   Published online August 31, 2020
DOI: https://doi.org/10.24171/j.phrp.2020.11.4.18
Corrects: Osong Public Health Res Perspect 2016;7(2):77
  • 3,275 View
  • 66 Download
  • 1 Citations
PDF
Traditional and Modern Cell Culture in Virus Diagnosis
Ali Hematian, Nourkhoda Sadeghifard, Reza Mohebi, Morovat Taherikalani, Abbas Nasrolahi, Mansour Amraei, Sobhan Ghafourian
Osong Public Health Res Perspect. 2016;7(2):77-82.   Published online April 30, 2016
DOI: https://doi.org/10.1016/j.phrp.2015.11.011
Correction in: Osong Public Health Res Perspect 2020;11(4):266
  • 2,970 View
  • 40 Download
  • 48 Citations
AbstractAbstract PDF
Cell cultures are developed from tissue samples and then disaggregated by mechanical, chemical, and enzymatic methods to extract cells suitable for isolation of viruses. With the recent advances in technology, cell culture is considered a gold standard for virus isolation. This paper reviews the evolution of cell culture methods and demonstrates why cell culture is a preferred method for identification of viruses. In addition, the advantages and disadvantages of both traditional and modern cell culture methods for diagnosis of each type of virus are discussed. Detection of viruses by the novel cell culture methods is considered more accurate and sensitive. However, there is a need to include some more accurate methods such as molecular methods in cell culture for precise identification of viruses.
Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei
Amir Ghasemi, Sobhan Ghafourian, Sedighe Vafaei, Reza Mohebi, Maryam Farzi, Morovat Taherikalani, Nourkhoda Sadeghifard
Osong Public Health Res Perspect. 2015;6(6):336-340.   Published online December 31, 2015
DOI: https://doi.org/10.1016/j.phrp.2015.10.003
  • 1,681 View
  • 20 Download
  • 3 Citations
AbstractAbstract PDF
Objectives
In an attempt α-amylase gene from Pyrococcus woesei was amplified and cloned into a pTYB2 vector to generate the recombinant plasmid pTY- α-amylase.
Methods
Escherichia coli BL21 used as a host and protein expression was applied using IPTG. SDS-PAGE assay demonstrated the 100 kDa protein. Amylolytic activity of proteins produced by transformed E. coli cells was detected by zymography, and the rate of active α-amylase with and without the intein tag in both soluble conditions and as inclusion bodies solubilized by 4M urea were measured.
Results
Amylolytic activity of ∼185,000 U/L of bacterial culture was observed from the soluble form of the protein using this system.
Conclusion
These results indicate that this expression system was appropriate for the production of thermostable α-amylase.

PHRP : Osong Public Health and Research Perspectives