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Reza Arefpajohi 1 Article
Evaluation of Mycobacterium tuberculosis Early Secreted Antigenic Target 6 Recombinant Protein as a Diagnostic Marker in Skin Test
Jale Moradi, Nader Mosavari, Mahmoud Ebrahimi, Reza Arefpajohi, Majid Tebianian
Osong Public Health Res Perspect. 2015;6(1):34-38.   Published online February 28, 2015
DOI: https://doi.org/10.1016/j.phrp.2014.12.002
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  • 5 Citations
AbstractAbstract PDF
Objectives
Tuberculosis (TB) is the leading infectious disease in the developing world. Delayed-type hypersensitivity skin test diagnoses TB using tuberculin purified protein derivative (PPD), but this test is incapable of distinguishing Mycobacterium tuberculosis (MTB) infection from bacillus Calmette–Guérin (BCG) vaccination or an infection caused by nontuberculous mycobacteria (NTM). This study was performed to evaluate the use of recombinant early secretory antigenic target 6 (rESAT-6), a secretory protein found only in MTB, Mycobacterium bovis, and few other mycobacterial species, as a skin marker for MTB in guinea pigs.
Methods
We prepared recombinant MTB ESAT-6 and evaluated its use as a specific antigen for MTB in guinea pigs.
Results
Our results show that the purified MTB rESAT-6 antigen is capable of inducing a positive reaction only in guinea pigs sensitized to MTB. No such reaction was observed in the animals sensitized to M. bovis, BCG vaccination, or NTM (Mycobacterium avium).
Conclusion
Our study results confirm that the ESAT-6 antigen is more specific to MTB infection than PPD and could be used in more specific skin tests for detection of MTB in large animals and in humans.

Citations

Citations to this article as recorded by  
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    Parasitology Research.2019; 118(1): 317.     CrossRef
  • Application of transdermal patches with new skin test reagents for detection of latent tuberculosis
    Songsri Kasempimolporn, Pannatat Areekul, Wichit Thaveekarn, Rattana Sutthisri, Supatsorn Boonchang, Artikaya Sawangvaree, Visith Sitprija
    Journal of Medical Microbiology.2019; 68(9): 1314.     CrossRef
  • Guinea pig infected with Mycobacterium tuberculosis via oral consumption
    Jianfeng Gao, Ming Guo, Li Teng, Rong Bao, Qiaoyang Xian, Xin Wang, Wenzhe Ho
    Journal of Applied Animal Research.2018; 46(1): 1323.     CrossRef
  • New strategy to improve quality control of Montenegro skin test at the production level
    Deborah Carbonera Guedes, João Carlos Minozzo, Aline Kuhn Sbruzzi Pasquali, Craig Faulds, Carlos Ricardo Soccol, Vanete Thomaz-Soccol
    Revista da Sociedade Brasileira de Medicina Tropic.2017; 50(6): 788.     CrossRef
  • Dissecting the mycobacterial cell envelope and defining the composition of the native mycomembrane
    Laura Chiaradia, Cyril Lefebvre, Julien Parra, Julien Marcoux, Odile Burlet-Schiltz, Gilles Etienne, Maryelle Tropis, Mamadou Daffé
    Scientific Reports.2017;[Epub]     CrossRef

PHRP : Osong Public Health and Research Perspectives