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Rabindra N. Padhy 4 Articles
Corrigendum to “Statistical Evaluation of Two Microbiological Diagnostic Methods of Pulmonary Tuberculosis After Implementation of a Directly Observed Treatment Short-course Program” [Osong Public Health Res Perspect 2013;4(1):45–51]
Shakti Rath, Debasmita Dubey, Mahesh C. Sahu, Sudhanshu S. Mishra, Rabindra N. Padhy
Osong Public Health Res Perspect. 2019;10(3):202-202.   Published online June 30, 2019
DOI: https://doi.org/10.24171/j.phrp.2019.10.3.11
  • 2,390 View
  • 32 Download
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In Vitro Antibacterial Activity, Gas Chromatography–Mass Spectrometry Analysis of Woodfordia fruticosa Kurz. Leaf Extract and Host Toxicity Testing With In Vitro Cultured Lymphocytes From Human Umbilical Cord Blood
Debasmita Dubey, Rajashree Patnaik, Goutam Ghosh, Rabindra N. Padhy
Osong Public Health Res Perspect. 2014;5(5):298-312.   Published online October 31, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.08.001
  • 1,918 View
  • 23 Download
  • 14 Citations
AbstractAbstract PDF
Objectives
To locate a plant with suitable phytochemicals for use as antimicrobial agents to control multidrug-resistant (MDR) bacteria as a complementary medicine, without host toxicity as monitored through cultured lymphocytes from human umbilical cord blood.
Methods
The methanol crude leaf extract of the plant Woodfordia fruticosa was subjected to antimicrobial assay in vitro with nine pathogenic MDR bacteria from clinical samples. This was followed by bioassay-guided fractionation with seven non-polar to polar solvents, gas chromatography–mass spectrometry analysis of the n-butanol fraction, and monitoring of the host toxicity of the leaf extract with in vitro grown lymphocytes from human umbilical cord blood.
Results
The leaf extract of W. fruticosa had a controlling capacity for MDR bacteria. The minimum inhibitory concentration and minimum bactericidal concentration of the n-butanol fraction were < 1.89 mg/mL extract and 9.63 mg/mL extract, respectively. The gas chromatography–mass spectrometry spectrum of the n-butanol fraction confirmed the presence of 13 peaks of different compounds with retention times of 9.11 minutes, 9.72 minutes, 10.13 minutes, 10.78 minutes, 12.37 minutes, 12.93 minutes, 18.16 minutes, 21.74 minutes, 21.84 minutes, 5.96 minutes, 12.93 minutes, 24.70 minutes, and 25.76 minutes. The six leading compounds were: diethyl phthalate: IUPAC name: diethyl benzene-1,2-dicarboxylate; 5-methyl-2-(1-methylethyl) phenol: IUPAC name: 5-methyl-2-propan-2-ylphenol; (E )-3,7-dimethylocta-2,6-diene-1-thiol: IUPAC name: (2Z)-3,7-dimethylocta-2,6-diene-1-thiol; 2,6,10-dodecatrien-1-ol, 3,7,11-trimethyl-, (E,E ): IUPAC name: 2,6,10-dodecatrien-1-ol; 3,7,11-trimethyl-, (E,E); 2-methoxy-4-(2-propenyl) phenol: IUPAC name: 2-methoxy-4-[(1E)-prop-1-en-1-yl]phenol; hexadecanoic acid: IUPAC name: hexadecanoic acid.
Conclusion
The presence of antimicrobial compounds that are therapeutically potent against MDR bacteria was confirmed in W. fruticosa. The crude leaf extract showed no host toxicity with human lymphocytes; the n-butanol fraction of the extract was the most suitable bioactive fraction. The terpenes isolated were: 5-methyl-2-(1-methylethyl) phenol, 2-methoxy-4-(2-propenyl) phenol, 2,6-octadien-1-ol, 3,7-dimethyl-(E)-2,6-octadienal, 3,7-dimethylcyclohexanol, and cyclohexanol, 2-methylene-5-(1-methylethenyl) which were reported to have specifically antimicrobial activity.

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  • Antibacterial activity of essential oils for combating colistin-resistant bacteria
    Abdullah M. Foda, Mohamed H. Kalaba, Gamal M. El-Sherbiny, Saad A. Moghannem, Esmail M. El-Fakharany
    Expert Review of Anti-infective Therapy.2022; 20(10): 1351.     CrossRef
  • Woodfordia fruticosa extract nanoemulsion: Influence of processing treatment on droplet size and its assessment for in vitro antimicrobial and anti-inflammatory activity
    Agnieszka Najda, Aarti Bains, Joanna Klepacka, Prince Chawla
    Frontiers in Nutrition.2022;[Epub]     CrossRef
  • Antioxidant-Rich Woodfordia fruticosa Leaf Extract Alleviates Depressive-Like Behaviors and Impede Hyperglycemia
    Mohammed Abu Tayab, Kazi Ashfak Ahmed Chowdhury, Md. Jabed, Syed Mohammed Tareq, A. T. M. Mostafa Kamal, Mohammad Nazmul Islam, A. M. Kafil Uddin, Mohammad Adil Hossain, Talha Bin Emran, Jesus Simal-Gandara
    Plants.2021; 10(2): 287.     CrossRef
  • Proteolytic enzyme arbitrated antagonization of helminthiasis by Cinnamomum cappara leaf extract in Pheretima posthuma
    Kayeen Vadakkan, Meena K Cheruvathur, Anu S Chulliparambil, Famy Francis, Anu P Abimannue
    Clinical Phytoscience.2021;[Epub]     CrossRef
  • Assessment of Anti-Inflammatory and Antimicrobial Potential of Ethanolic Extract of Woodfordia fruticosa Flowers: GC-MS Analysis
    Agnieszka Najda, Aarti Bains, Prince Chawla, Anil Kumar, Sebastian Balant, Magdalena Walasek-Janusz, Dariusz Wach, Ravinder Kaushik
    Molecules.2021; 26(23): 7193.     CrossRef
  • Plant-Based Phytochemicals as Possible Alternative to Antibiotics in Combating Bacterial Drug Resistance
    Hana Mohammed Al AlSheikh, Insha Sultan, Vijay Kumar, Irfan A. Rather, Hashem Al-Sheikh, Arif Tasleem Jan, Qazi Mohd Rizwanul Haq
    Antibiotics.2020; 9(8): 480.     CrossRef
  • Ethnomedicinal Uses of Plant Resources in the Machhapuchchhre Rural Municipality of Kaski District, Nepal
    Mahendra Adhikari, Rashmi Thapa, Ripu Mardhan Kunwar, Hari Prasad Devkota, Prakash Poudel
    Medicines.2019; 6(2): 69.     CrossRef
  • Screening of in vitro antimicrobial activity of plants used in traditional Indonesian medicine
    Andreas Romulo, Ervizal A. M. Zuhud, Johana Rondevaldova, Ladislav Kokoska
    Pharmaceutical Biology.2018; 56(1): 287.     CrossRef
  • GC MS Analysis of One Ayurvedic Preparation ‘Aswagandharishtam
    M. Kotteswari, M. R. K. Rao, Siva Kumar, K. Prabhu, R. Lakshmi Sundaram, Shruthi Dinakar
    Biomedical and Pharmacology Journal.2018; 11(2): 1061.     CrossRef
  • Phytochemical constituents, antioxidant and antibacterial activities of methanolic extract of Ardisia elliptica
    Nazeh M. Al-Abd, Zurainee Mohamed Nor, Marzida Mansor, Asdren Zajmi, Mohd Shahnaz Hasan, Fadzly Azhar, Mustafa Kassim
    Asian Pacific Journal of Tropical Biomedicine.2017; 7(6): 569.     CrossRef
  • In vitro antibacterial activity of crude extracts of 9 selected medicinal plants against UTI causing MDR bacteria
    Monali P. Mishra, Sibanarayan Rath, Shasank S. Swain, Goutam Ghosh, Debajyoti Das, Rabindra N. Padhy
    Journal of King Saud University - Science.2017; 29(1): 84.     CrossRef
  • Antiviral activity of selected medicinal plants and marine seaweeds on the grasserie infected larvae of silkworm, Bombyx mori
    Chitra Somu, Ramaraj Paulchamy, S. M. Moorthy, Janarthanan Sundaram
    Archives of Phytopathology and Plant Protection.2017; 50(17-18): 850.     CrossRef
  • Screening and antibacterial efficacy of selected Indian medicinal plants
    Suresh Mickymaray, Mohammad Saleh Al Aboody, Pradipta Kumar Rath, Panneerselvam Annamalai, Thajuddin Nooruddin
    Asian Pacific Journal of Tropical Biomedicine.2016; 6(3): 185.     CrossRef
  • Ellagic acid: Pharmacological activities and molecular mechanisms involved in liver protection
    Wylly Ramsés García-Niño, Cecilia Zazueta
    Pharmacological Research.2015; 97: 84.     CrossRef
In Vitro Antibacterial Efficacy of 21 Indian Timber-Yielding Plants Against Multidrug-Resistant Bacteria Causing Urinary Tract Infection
Monali P. Mishra, Rabindra N. Padhy
Osong Public Health Res Perspect. 2013;4(6):347-357.   Published online December 31, 2013
DOI: https://doi.org/10.1016/j.phrp.2013.10.007
  • 2,000 View
  • 12 Download
  • 18 Citations
AbstractAbstract PDF
Objectives
To screen methanolic leaf extracts of 21 timber-yielding plants for antibacterial activity against nine species of uropathogenic bacteria isolated from clinical samples of a hospital (Enterococcus faecalis, Staphylococcus aureus, Acinetobacter baumannii, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa).
Methods
Bacterial strains were subjected to antibiotic sensitivity tests by the Kirby–Bauer's disc diffusion method. The antibacterial potentiality of leaf extracts was monitored by the agar-well diffusion method with multidrug-resistant (MDR) strains of nine uropathogens.
Results
Two Gram-positive isolates, E. faecalis and S. aureus, were resistant to 14 of the 18 antibiotics used. Gram-negative isolates A. baumannii, C. freundii, E. aerogenes, E. coli, K. pneumoniae, P. mirabilis, and P. aeruginosa were resistant to 10, 12, 9, 11, 11, 10, and 11 antibiotics, respectively, of the 14 antibiotics used. Methanolic leaf extracts of Anogeissus acuminata had the maximum zone of inhibition size—29 mm against S. aureus and 28 mm against E. faecalis and P. aeruginosa. Cassia tora had 29 mm as the zone of inhibition size for E. faecalis, E. aerogenes, and P. aeruginosa. Based on the minimum inhibitory concentration and minimum bactericidal concentration values, the most effective 10 plants against uropathogens could be arranged in decreasing order as follows: C. tora > A. acuminata > Schleichera oleosa > Pterocarpus santalinus > Eugenia jambolana > Bridelia retusa > Mimusops elengi > Stereospermum kunthianum > Tectona grandis > Anthocephalus cadamba. The following eight plants had moderate control capacity: Artocarpus heterophyllus, Azadirachta indica, Dalbergia latifolia, Eucalyptus citriodora, Gmelina arborea, Pongamia pinnata, Pterocarpus marsupium, and Shorea robusta. E. coli, followed by A. baumannii, C. freundii, E. aerogenes, P. mirabilis, and P. aeruginosa were controlled by higher amounts/levels of leaf extracts. Phytochemicals of all plants were qualitatively estimated.
Conclusions
A majority of timber-yielding plants studied had in vitro control capacity against MDR uropathogenic bacteria.

Citations

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    Arabian Journal of Chemistry.2022; 15(2): 103601.     CrossRef
  • Evaluation of the sensory attributes of pepper soup beef hides and determination of the preservative potential of the spices used for its preparation
    T.C.L. Maguipa, P.D. Mbougueng, H.M. Womeni
    Journal of Agriculture and Food Research.2022; 8: 100293.     CrossRef
  • Flourensia retinophylla: An outstanding plant from northern Mexico with antibacterial activity
    D. Jasso de Rodríguez, M.C. Victorino-Jasso, N.E. Rocha-Guzmán, M.R. Moreno-Jiménez, L. Díaz-Jiménez, R. Rodríguez-García, J.Á. Villarreal-Quintanilla, F.M. Peña-Ramos, D.A. Carrillo-Lomelí, Z.A. Genisheva, M.L. Flores-López
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  • Computational in Silico Modelling of Phytochemicals as a Potential Cure
    Rachita Kurmi, Kavya N R, Jennath Sherin A, Silpa T S
    International Journal of Scientific Research in Sc.2021; : 42.     CrossRef
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    Hanyan Luo, Hongwei Wu, Lixia Wang, Shuiming Xiao, Yaqi Lu, Cong Liu, Xiankuo Yu, Xiao Zhang, Zhuju Wang, Liying Tang
    Communications Biology.2021;[Epub]     CrossRef
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    Parul Kaushik, Priyanka Ahlawat, Kuldeep Singh, Raman Singh
    Advances in Traditional Medicine.2021;[Epub]     CrossRef
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    Amina A. Aly, Hoda G. M. Ali, Noha E. R. Eliwa
    Journal of Food Measurement and Characterization.2019; 13(2): 911.     CrossRef
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    Sujogya Kumar Panda, Yugal Kishore Mohanta, Laxmipriya Padhi, Walter Luyten
    LWT.2019; 113: 108246.     CrossRef
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    Mlatovi Dégbé, Françoise Debierre-Grockiego, Amivi Tété-Bénissan, Héloïse Débare, Kodjo Aklikokou, Isabelle Dimier-Poisson, Messanvi Gbeassor
    Parasite.2018; 25: 11.     CrossRef
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    D. Jasso de Rodríguez, L.C. García-Hernández, N.E. Rocha-Guzmán, M.R. Moreno-Jiménez, R. Rodríguez-García, M.L.V. Díaz-Jiménez, A. Sáenz-Galindo, J.A. Villarreal-Quintanilla, F.M. Peña-Ramos, M.L. Flores-López, D.A. Carrillo-Lomelí
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    Ramaswamy Malathi, Solaimuthu Chandrasek
    Research Journal of Medicinal Plants.2017; 11(3): 93.     CrossRef
  • In vitro antibacterial activity of crude extracts of 9 selected medicinal plants against UTI causing MDR bacteria
    Monali P. Mishra, Sibanarayan Rath, Shasank S. Swain, Goutam Ghosh, Debajyoti Das, Rabindra N. Padhy
    Journal of King Saud University - Science.2017; 29(1): 84.     CrossRef
  • Phytochemical investigation and antimicrobial assessment of Bellis sylvestris leaves
    Monica Scognamiglio, Elisabetta Buommino, Lorena Coretti, Vittoria Graziani, Rosita Russo, Pina Caputo, Giovanna Donnarumma, Brigida D⿿Abrosca, Antonio Fiorentino
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    Asian Pacific Journal of Tropical Biomedicine.2015; 5(11): 928.     CrossRef
  • In vitro antibacterial efficacy of plants used by an Indian aboriginal tribe against pathogenic bacteria isolated from clinical samples
    Shasank S. Swain, Rabindra N. Padhy
    Journal of Taibah University Medical Sciences.2015; 10(4): 379.     CrossRef
  • Monitoring in vitro antibacterial efficacy of 26 Indian spices against multidrug resistant urinary tract infecting bacteria
    Sibanarayan Rath, Rabindra N. Padhy
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Statistical Evaluation of Two Microbiological Diagnostic Methods of Pulmonary Tuberculosis After Implementation of a Directly Observed Treatment Short-course Program
Shakti Rath, Debasmita Dubey, Mahesh C. Sahu, Sudhanshu S. Mishra, Rabindra N. Padhy
Osong Public Health Res Perspect. 2013;4(1):45-51.   Published online February 28, 2013
DOI: https://doi.org/10.1016/j.phrp.2012.12.004
  • 2,072 View
  • 16 Download
  • 2 Citations
AbstractAbstract PDF
Objectives
To evaluate the diagnostic accuracy of smear and culture tests of clinical samples of pulmonary tuberculosis after the introduction of the directly observed treatment short-course (DOTS) program.
Methods
Using sputum samples from 572 individuals as a self-selected population, both Ziehl–Neelsen staining and culturing on Lowenstein–Jensen medium were carried out as diagnostic procedures. Using Bayes’ rule, the obtained data set was analyzed.
Results
Of the 572 samples, 33 (0.05769) were true positive (results of both tests positive) cases; 22 samples (0.03846) were false positive (smear test positive and culture test negative) cases; 62 samples (0.10839) were false negative (smear test negative and culture test positive) cases; and 455 samples (0.79545) were true negative (results of both tests negative) cases. Values of test statistics, sensitivity, and specificity were used to compute several inherent other Bayesian test statistics. The a priori probability or prevalence value of tuberculosis in the targeted population was 0.166. The a posteriori probability value computed arithmetically was 0.6614 and that obtained by the graphical method was 0.62.
Conclusions
The smear test was found to be dependable for 95.4% with stable TB infections, and it was not dependable for 34.7% without stable TB infections. The culture test could be regarded as the gold standard for 96.15% as seen with the data set, which was obtained after the implementation of the DOTS program.

Citations

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  • Comprehensive Determination of Mycobacterium tuberculosis and Nontuberculous Mycobacteria From Targeted Capture Sequencing
    Ya He, Ziying Gong, Xiaokai Zhao, Daoyun Zhang, Zhongshun Zhang
    Frontiers in Cellular and Infection Microbiology.2020;[Epub]     CrossRef
  • Primary tuberculosis of the glans penis-a rare case report
    Rajashree Panigrahy, Suren Kumar Das, Subhrajita Rout, Mahesh Chandra Sahu, Rabindra Nath Padhy
    Asian Pacific Journal of Tropical Disease.2014; 4: S653.     CrossRef

PHRP : Osong Public Health and Research Perspectives