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Nara Lim 2 Articles
Molecular Typing of Mycobacterium intracellulare Using Pulsed-Field Gel Electrophoresis, Variable-Number Tandem-Repeat Analysis, Mycobacteria Interspersed Repetitive-Unit-Variable-Number Tandem Repeat Typing, and Multilocus Sequence Typing: Molecular Characterization and Comparison of Each Typing Methods
Semi Jeon, Nara Lim, Seungjik Kwon, Taesun Shim, Misun Park, Bum-Joon Kim, Seonghan Kim
Osong Public Health Res Perspect. 2014;5(3):119-130.   Published online June 30, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.04.003
  • 1,899 View
  • 14 Download
  • 8 Citations
AbstractAbstract PDF
Objectives
Mycobacterium intracellulare is the major causative agent of nontuberculous mycobacteria-related pulmonary infections. The strain typing of M. intracellulare is important for the treatment and control of its infections. We compared the discrimination capacity and effective value of four different molecular typing methods.
Methods
Antibiotic susceptibility testing, hsp65 and rpoB sequencing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), mycobacteria interspersed repetitive-unit-variable-number tandem-repeat analysis (MIRU-VNTR), and VNTR assay targeting 44 M. intracellulare isolates obtained from patients with pulmonary infections were performed.
Results
All the antibiotic susceptibility patterns had no association with the molecular and sequence types tested in this study; however, the molecular and sequence types were related with each other. PFGE gave best results for discriminatory capacity, followed by VNTR, MLST, and MIRU-VNTR.
Conclusion
The high discriminatory power of PFGE, VNTR, and MLST is enough for differentiating between reinfection and relapse, as well as for other molecular epidemiological usages. The MLST could be regarded as a representative classification method, because it showed the clearest relation with the sequence types.

Citations

Citations to this article as recorded by  
  • Differential Genotyping of Mycobacterium avium Complex and Its Implications in Clinical and Environmental Epidemiology
    Jeong-Ih Shin, Sung Jae Shin, Min-Kyoung Shin
    Microorganisms.2020; 8(1): 98.     CrossRef
  • A strategy based on Amplified Fragment Length Polymorphism (AFLP) for routine genotyping of nontuberculous mycobacteria at the clinical laboratory
    Sara Blanco-Conde, Carolina González-Cortés, Ramiro López-Medrano, Juan José Palacios-Gutiérrez, Cristina Diez-Tascón, Teresa Nebreda-Mayoral, María Josefa Sierra-García, Octavio Miguel Rivero-Lezcano
    Molecular Biology Reports.2020; 47(5): 3397.     CrossRef
  • Comparative Evaluation of Band-Based Genotyping Methods for Mycobacterium intracellulare and Its Application for Epidemiological Analysis
    Jeong-Ih Shin, Jong-Hun Ha, Dong-Hae Lee, Jeong-Gyu Choi, Kyu-Min Kim, Seung Jun Lee, Yi Yeong Jeong, Jong Deog Lee, Myunghwan Jung, Seung-Chul Baik, Woo Kon Lee, Hyung-Lyun Kang, Min-Kyoung Shin, Jung-Wan Yoo
    Microorganisms.2020; 8(9): 1315.     CrossRef
  • Pulsed Field Gel Electrophoresis: Past, present, and future
    Lilia Lopez-Canovas, Maximo B. Martinez Benitez, Jose A. Herrera Isidron, Eduardo Flores Soto
    Analytical Biochemistry.2019; 573: 17.     CrossRef
  • Molecular typing of Mycobacterium kansasii using pulsed-field gel electrophoresis and a newly designed variable-number tandem repeat analysis
    Zofia Bakuła, Anna Brzostek, Paulina Borówka, Anna Żaczek, Izabela Szulc-Kiełbik, Agata Podpora, Paweł Parniewski, Dominik Strapagiel, Jarosław Dziadek, Małgorzata Proboszcz, Jacek Bielecki, Jakko van Ingen, Tomasz Jagielski
    Scientific Reports.2018;[Epub]     CrossRef
  • Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare
    So-Young Lee, Byoung-Jun Kim, Hong Kim, Yu-Seop Won, Che Ok Jeon, Joseph Jeong, Seon Ho Lee, Ji-Hun Lim, Seung-Heon Lee, Chang Ki Kim, Yoon-Hoh Kook, Bum-Joon Kim
    International Journal of Systematic and Evolution.2016; 66(8): 3132.     CrossRef
  • Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria
    Tomasz Jagielski, Alina Minias, Jakko van Ingen, Nalin Rastogi, Anna Brzostek, Anna Żaczek, Jarosław Dziadek
    Clinical Microbiology Reviews.2016; 29(2): 239.     CrossRef
  • Genetic diversity of clinical Mycobacterium avium subsp. hominissuis and Mycobacterium intracellulare isolates causing pulmonary diseases recovered from different geographical regions
    Kazuya Ichikawa, Jakko van Ingen, Won-Jung Koh, Dirk Wagner, Max Salfinger, Takayuki Inagaki, Kei-ichi Uchiya, Taku Nakagawa, Kenji Ogawa, Kiyofumi Yamada, Tetsuya Yagi
    Infection, Genetics and Evolution.2015; 36: 250.     CrossRef
Resistance to Fluoroquinolone by a Combination of Efflux and Target Site Mutations in Enteroaggregative Escherichia coli Isolated in Korea
Jun-Young Kim, Se-Mi Jeon, Hyungjun Kim, Nara Lim, Mi-Sun Park, Seong-Han Kim
Osong Public Health Res Perspect. 2012;3(4):239-244.   Published online December 31, 2012
DOI: https://doi.org/10.1016/j.phrp.2012.11.002
  • 1,884 View
  • 16 Download
  • 8 Citations
AbstractAbstract PDF
Objectives
Enteroaggregative Escherichia coli (EAEC) was recently reported as a major diarrheagenic pathogen in infant and adult travelers, both in developing and developed countries. EAEC strains are known to be highly resistant to antibiotics including quinolones. Therefore in this study we have determined the various mechanisms of quinolone resistance in EAEC strains isolated in Korea.
Methods
For 26 EAEC strains highly resistant to fluoroquinolone, minimal inhibitory concentrations for fluoroquinolones were determined, mutations in the quinolone target genes were identified by PCR and sequencing, the presence of transferable quinolone resistance mechanism were identified by PCR, and the contribution of the efflux pump was determined by synergy tests using a proton pump inhibitor. The expression levels of efflux pump-related genes were identified by relative quantification using real-time PCR.
Results
Apart from two, all tested isolates had common mutations on GyrA (Ser83Leu and Ser87Gly) and ParC (Ser80Gln). Isolates EACR24 and EACR39 had mutations that have not been reported previously: Ala81Pro in ParC and Arg157Gly in GyrA, respectively. Increased susceptibility of all the tested isolates to ciprofloxacin and norfloxacin in the presence of the pump inhibitor implies that efflux pumps contributed to the resistance against fluoroquinolones. Expression of the efflux pump-related genes, tolC, mdfA, and ydhE, were induced in isolates EACR 07, EACR 29, and EACR 33 in the presence of ciprofloxacin.
Conclusion
These results indicate that quinolone resistance of EAEC strains mainly results from the combination of mutations in the target enzyme and an increased expression of efflux pump-related genes. The mutations Ala81Pro in ParC and Arg157Gly in GyrA have not been reported previously the exact influence of these mutations should be investigated further.

Citations

Citations to this article as recorded by  
  • Detection of gyrA and parC Mutations and Prevalence of Plasmid-Mediated Quinolone Resistance Genes in Klebsiella pneumoniae
    Sawsan Mohammed Kareem, Israa MS Al-kadmy, Saba S Kazaal, Alaa N Mohammed Ali, Sarah Naji Aziz, Rabab R Makharita, Abdelazeem M Algammal, Salim Al-Rejaie, Tapan Behl, Gaber El-Saber Batiha, Mohamed A El-Mokhtar, Helal F Hetta
    Infection and Drug Resistance.2021; Volume 14: 555.     CrossRef
  • Fluoroquinolone-Transition Metal Complexes: A Strategy to Overcome Bacterial Resistance
    Mariana Ferreira, Paula Gameiro
    Microorganisms.2021; 9(7): 1506.     CrossRef
  • Frequency of DNA gyrase and topoisomerase IV mutations and plasmid-mediated quinolone resistance genes among Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections in Azerbaijan, Iran
    Robab Azargun, Mohammad Hossein Soroush Barhaghi, Hossein Samadi Kafil, Mahin Ahangar Oskouee, Vahid Sadeghi, Mohammad Yousef Memar, Reza Ghotaslou
    Journal of Global Antimicrobial Resistance.2019; 17: 39.     CrossRef
  • Evaluating the efficacy of an algae-based treatment to mitigate elicitation of antibiotic resistance
    Kassandra L. Grimes, Laura J. Dunphy, Erica M. Loudermilk, A. Jasmin Melara, Glynis L. Kolling, Jason A. Papin, Lisa M. Colosi
    Chemosphere.2019; 237: 124421.     CrossRef
  • Resistance mechanisms of Helicobacter pylori and its dual target precise therapy
    Yuehua Gong, Yuan Yuan
    Critical Reviews in Microbiology.2018; 44(3): 371.     CrossRef
  • E. coli Group 1 Capsular Polysaccharide Exportation Nanomachinary as a Plausible Antivirulence Target in the Perspective of Emerging Antimicrobial Resistance
    Shivangi Sachdeva, Raghuvamsi V. Palur, Karpagam U. Sudhakar, Thenmalarchelvi Rathinavelan
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • Plasmid-Mediated Quinolone Resistance in Different Diarrheagenic Escherichia coli Pathotypes Responsible for Complicated, Noncomplicated, and Traveler's Diarrhea Cases
    Silvia Herrera-León, María Teresa Llorente, Sergio Sánchez
    Antimicrobial Agents and Chemotherapy.2016; 60(3): 1950.     CrossRef
  • Determinants of quinolone resistance in Escherichia coli causing community-acquired urinary tract infection in Bejaia, Algeria
    Yanat Betitra, Vinuesa Teresa, Viñas Miguel, Touati Abdelaziz
    Asian Pacific Journal of Tropical Medicine.2014; 7(6): 462.     CrossRef

PHRP : Osong Public Health and Research Perspectives