Osong Public Health and Research Perspectives

Nan-Ok Kim

4 Articles

Genome Sequencing Analysis of Atypical Shigella flexneri Isolated in Korea: Nan-Ok Kim, Hae-young Na, Su-Mi Jung, Gyung Tae Chung, Hyo Sun Kawk, Sahyun Hong; Osong Public Health Res Perspect. 2017;8(1):78-85. Published online February 28, 2017; DOI: https://doi.org/10.24171/j.phrp.2017.8.1.11

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Objectives

An atypical Shigella flexneri strain with a plural agglutination pattern [i.e., reacting not only with serum samples containing type antigen II but also with serum samples containing group antigens (3)4 and 7(8)] was selected for genome sequencing, with the aim of obtaining additional comparative information about such strains.

Methods

The genomic DNA of atypical S. flexneri strain NCCP 15744 was sequenced using an Ion Torrent PGM sequencing machine (Life Technologies, USA). The raw sequence data were preprocessed and reference-assembled in the CLC Assembly Cell software (version 4.0.6; CLC bio, USA).

Results

Ion Torrent sequencing produced 1,450,025 single reads with an average length of 144 bp, totaling ~209 Mbp. The NCCP 15744 genome is composed of one chromosome and four plasmids and contains a gtrX gene. Among the published genome sequences of S. flexneri strains, including 2457T, Sf301, and 2002017, strain NCCP 15744 showed high similarity with strain 2002017. The differences between NCCP 15744 and 2002017 are as follows: i) NCCP 15744 carries four plasmids whereas 2002017 carries five; ii) 19 genes (including CI, CII, and cro) were lost in the SHI-O genomic island of NCCP 15744 and six genes were gained as compared with strain 2002017.

Conclusion

Strain NCCP 15744 is genetically similar to 2002017, but these two strains have different multilocus sequence types and serotypes. The exact reason is unclear, but the 19 lost genes may be responsible for the atypical seroconversion of strain NCCP 15744.

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Genomic and proteomic characterization of two strains of Shigella flexneri 2 isolated from infants’ stool samples in Argentina
Mónica F. Torrez Lamberti, Lucrecia C. Terán, Fabián E. Lopez, María de las Mercedes Pescaretti, Mónica A. Delgado
BMC Genomics.2022;[Epub] CrossRef

Surveillance of Bacillus cereus Isolates in Korea from 2012 to 2014: Su-Mi Jung, Nan-Ok Kim, Injun Cha, Hae-young Na, Gyung Tae Chung, Hyo Sun Kawk, Sahyun Hong; Osong Public Health Res Perspect. 2017;8(1):71-77. Published online February 28, 2017; DOI: https://doi.org/10.24171/j.phrp.2017.8.1.10

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Abstract PDF

Objectives

To investigate the prevalence and toxin production characteristics of non-emetic and emetic Bacillus cereus strains isolated via the laboratory surveillance system in Korea.

Methods

A total of 667 B. cereus strains were collected by the Korea National Research Institute of Health laboratory surveillance system from 2012 to 2014. The collected strains were analyzed by geographical region, season, patient age, and patient sex. Additionally, the prevalence rates of enterotoxin and emetic toxin genes were evaluated.

Results

The isolation rate of B. cereus strains increased during the summer, but the isolation rate was evenly distributed among patient age groups. Emetic toxin was produced by 20.2% of the isolated strains. The prevalence rates of five enterotoxin genes (entFM, nheA, cytK2, hblC, and bceT) were 85.0, 78.6, 44.5, 36.6, and 29.7%, respectively, among non-emetic strains and 77.8, 59.3, 17.8, 11.9 and 12.6%, respectively, among emetic strains. Thus, the prevalence rates of all five enterotoxin genes were lower in emetic B. cereus.

Conclusion

The prevalence of enterotoxin genes differed between non-emetic and emetic B. cereus strains. Among emetic B. cereus strains, the prevalence rates of two enterotoxin genes (cytK2 and hblC) were lower than those among the non-emetic strains. In both the emetic and non-emetic strains isolated in Korea, nheA and entFM were the most prevalent enterotoxin genes.

Citations

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A study on toxin genes and cytotoxity levels of Bacillus cereus in various ready to eat foods and pastry products in Turkey
Başak Gökçe ÇÖL, Harun AKSU
Journal of Istanbul Veterinary Sciences.2022; 6(3): 152. CrossRef
Autoinducer-2 Could Affect Biofilm Formation by Food-Derived Bacillus cereus
Nari Lee, Myo-Deok Kim, Min-Cheol Lim
Indian Journal of Microbiology.2021; 61(1): 66. CrossRef
Effects of Low-Temperature Drying with Intermittent Gaseous Chlorine Dioxide Treatment on Texture and Shelf-Life of Rice Cakes
Timilehin Martins Oyinloye, Won Byong Yoon
Processes.2020; 8(3): 375. CrossRef

Enteric Bacteria Isolated from Diarrheal Patients in Korea in 2014: Nan-Ok Kim, Su-Mi Jung, Hae-Young Na, Gyung Tae Chung, Cheon-Kwon Yoo, Won Keun Seong, Sahyun Hong; Osong Public Health Res Perspect. 2015;6(4):233-240. Published online August 31, 2015; DOI: https://doi.org/10.1016/j.phrp.2015.07.005

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11 Citations

Abstract PDF Supplementary Material

Objectives
The aim of this study was to characterize the pathogens responsible for causing diarrhea according to season, region of isolation, patient age, and sex as well as to provide useful data for the prevention of diarrheal disease.
Methods
Stool specimens from 14,886 patients with diarrhea were collected to identify pathogenic bacteria from January 2014 to December 2014 in Korea. A total of 3,526 pathogenic bacteria were isolated and analyzed according to season, region of isolation, and the age and sex of the patient.
Results
The breakdown of the isolated pathogenic bacteria were as follows: Salmonella spp. 476 (13.5%), pathogenic Escherichia coli 777 (22.0%), Vibrio parahaemolyticus 26 (0.74%), Shigella spp. 13 (0.37%), Campylobacter spp. 215 (6.10%), Clostridium perfringens 508 (14.4%), Staphylococcus aureus 1,144 (32.4%), Bacillus cereus 356 (10.1%), Listeria monocytogenes 1 (0.03%), and Yersinia enterocolitica 10 (0.3%). The isolation rate trend showed the highest ratio in the summer season from June to September for most of the pathogenic bacteria except the Gram-positive bacteria. The isolation rate of most of the pathogenic bacteria by patient age showed highest ratio in the 0–19 year age range. For isolation rate by region, 56.2% were isolated from cities and 43.8% were isolated from provinces.
Conclusion
Hygiene education should be addressed for diarrheal disease-susceptible groups, such as those younger than 10 years, aged 10–19 years, and older than 70 years, and monitoring for the pathogens is still required. In addition, an efficient laboratory surveillance system for infection control should be continued.

Citations

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Plant-derived nanoparticles as alternative therapy against Diarrheal pathogens in the era of antimicrobial resistance: A review
Tesleem Olatunde Abolarinwa, Daniel Jesuwenu Ajose, Bukola Opeyemi Oluwarinde, Justine Fri, Kotsoana Peter Montso, Omolola Esther Fayemi, Adeyemi Oladapo Aremu, Collins Njie Ateba
Frontiers in Microbiology.2022;[Epub] CrossRef
Prevalence, co-infection and seasonality of fecal enteropathogens from diarrheic cats in the Republic of Korea (2016–2019): a retrospective study
Ye-In Oh, Kyoung-Won Seo, Do-Hyung Kim, Doo-Sung Cheon
BMC Veterinary Research.2021;[Epub] CrossRef
Genotyping and antimicrobial susceptibility of Clostridium perfringens isolated from dromedary camels, pastures and herders
Mahmoud Fayez, Ibrahim Elsohaby, Theeb Al-Marri, Kamal Zidan, Ali Aldoweriej, Elham El-Sergany, Ahmed Elmoslemany
Comparative Immunology, Microbiology and Infectiou.2020; 70: 101460. CrossRef
Comparative Analysis of Aerotolerance, Antibiotic Resistance, and Virulence Gene Prevalence in Campylobacter jejuni Isolates from Retail Raw Chicken and Duck Meat in South Korea
Jinshil Kim, Hyeeun Park, Junhyung Kim, Jong Hyun Kim, Jae In Jung, Seongbeom Cho, Sangryeol Ryu, Byeonghwa Jeon
Microorganisms.2019; 7(10): 433. CrossRef
Guideline for the Antibiotic Use in Acute Gastroenteritis
Youn Jeong Kim, Ki-Ho Park, Dong-Ah Park, Joonhong Park, Byoung Wook Bang, Seung Soon Lee, Eun Jung Lee, Hyo-Jin Lee, Sung Kwan Hong, Yang Ree Kim
Infection & Chemotherapy.2019; 51(2): 217. CrossRef
Winning the War against Multi-Drug Resistant Diarrhoeagenic Bacteria
Chizoba Mercy Enemchukwu, Angus Nnamdi Oli, Ebere Innocent Okoye, Nonye Treasure Ujam, Emmanuel O. Osazuwa, George Ogonna Emechebe, Kenneth Nchekwube Okeke, Christian Chukwuemeka Ifezulike, Obiora Shedrack Ejiofor, Jude Nnaemeka Okoyeh
Microorganisms.2019; 7(7): 197. CrossRef
Molecular Characterization of Enterotoxigenic Escherichia coli in Foodborne Outbreak
Sang-Hun Park, Hyun-Jung Seung, Hyo-Won Jeong, So-Yun Park, Ji-Hun Jung, Young-Hee Jin, Sung-Hee Han, Hee-Soon Kim, Jin-Seok Kim, Joo-Hyun Park, Ye-Ji Gong, Chae Kyu Hong, Jib-Ho Lee, Il-Young Kim, Kweon Jung
Journal of Bacteriology and Virology.2018; 48(4): 113. CrossRef
Epidemiology of Listeria monocytogenes prevalence in foods, animals and human origin from Iran: a systematic review and meta-analysis
Reza Ranjbar, Mehrdad Halaji
BMC Public Health.2018;[Epub] CrossRef
The Epidemiological Influence of Climatic Factors on Shigellosis Incidence Rates in Korea
Yeong-Jun Song, Hae-Kwan Cheong, Myung Ki, Ji-Yeon Shin, Seung-sik Hwang, Mira Park, Moran Ki, Jiseun Lim
International Journal of Environmental Research an.2018; 15(10): 2209. CrossRef
Molecular characterization and antimicrobial resistance profile of Clostridium perfringens type A isolates from humans, animals, fish and their environment
Jay Prakash Yadav, Suresh Chandra Das, Pankaj Dhaka, Deepthi Vijay, Manesh Kumar, Asish Kumar Mukhopadhyay, Goutam Chowdhury, Pranav Chauhan, Rahul Singh, Kuldeep Dhama, Satya Veer Singh Malik, Ashok Kumar
Anaerobe.2017; 47: 120. CrossRef
Molecular characterization of antimicrobial resistant non-typhoidal Salmonella from poultry industries in Korea
Jin Eui Kim, Young ju Lee
Irish Veterinary Journal.2017;[Epub] CrossRef

Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8): Sahyun Hong, Injun Cha, Nan-Ok Kim, Seong-Han Kim, Kyung-Tae Jung, Je-Hee Lee, Dong-Wook Kim, Mi-Sun Park, Yeon-Ho Kang; Osong Public Health Res Perspect. 2012;3(4):222-228. Published online December 31, 2012; DOI: https://doi.org/10.1016/j.phrp.2012.10.002

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Abstract PDF: Objectives
We aimed at evaluating the virulence of atypical Shigella flexneri II:(3)4,7(8) by DNA microarray and invasion assay.
Methods
We used a customized S. flexneri DNA microarray to analyze an atypical S. flexneri II:(3)4,7(8) gene expression profile and compared it with that of the S. flexneri 2b strain.
Results
Approximately one-quarter of the atypical S. flexneri II:(3)4,7(8) strain genes showed significantly altered expression profiles; 344 genes were more than two-fold upregulated, and 442 genes were more than 0.5-fold downregulated. The upregulated genes were divided into the category of 21 clusters of orthologous groups (COGs), and the “not in COGs” category included 170 genes. This category had virulence plasmid genes, including the ipa-mxi-spa genes required for invasion of colorectal epithelium (type III secretion system). Quantitative reverse-transcription polymerase chain reaction results also showed the same pattern in two more atypical S. flexneri II:(3)4,7(8) strains. Atypical S. flexneri II:(3)4,7(8) showed four times increased invasion activity in Caco-2 cells than that of typical strains.
Conclusion
Our results provide the intracellularly regulated genes that may be important for adaptation and growth strategies of this atypical S. flexneri.

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