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Mohsen Mohammadi 3 Articles
Analysis of Resistance to Macrolide–Lincosamide–Streptogramin B Among mecA-Positive Staphylococcus Aureus Isolates
Mahmoud Khodabandeh, Mohsen Mohammadi, Mohammad Reza Abdolsalehi, Azadeh Alvandimanesh, Mehrdad Gholami, Meysam Hasannejad Bibalan, Abazar Pournajaf, Ramin Kafshgari, Ramazan Rajabnia
Osong Public Health Res Perspect. 2019;10(1):25-31.   Published online February 28, 2019
DOI: https://doi.org/10.24171/j.phrp.2019.10.1.06
  • 3,865 View
  • 165 Download
  • 18 Citations
AbstractAbstract PDF
Objectives

Genetic determinants conferring resistance to macrolide, lincosamide, and streptogramin B (MLSB) via ribosomal modification such as, erm, msrA/B and ereA/B genes are distributed in bacteria. The main goals of this work were to evaluate the dissemination of MLSB resistance phenotypes and genotypes in methicillin-resistant Staphylococcus aureus (MRSA) isolates collected from clinical samples.

Methods

A total of 106 MRSA isolates were studied. Isolates were recovered from 3 hospitals in Tehran between May 2016 to July 2017. The prevalence of MLSB-resistant strains were determined by D-test, and then M-PCR was performed to identify genes encoding resistance to macrolides, lincosamides, and streptogramins in the tested isolates.

Results

The frequency of constitutive resistance MLSB, inducible resistance MLSB and MSB resistance were 56.2%, 22.9%, and 16.6%, respectively. Of 11 isolates with the inducible resistance MLSB phenotype, ermC, ermB, ermA and ereA were positive in 81.8%, 63.6%, 54.5% and 18.2% of these isolates, respectively. In isolates with the constitutive resistance MLSB phenotype, the prevalence of ermA, ermB, ermC, msrA, msrB, ereA and ereB were 25.9%, 18.5%, 44.4%, 0.0%, 0.0%, 11.1% and 0.0%, respectively.

Conclusion

Clindamycin is commonly administered in severe MRSA infections depending upon the antimicrobial susceptibility findings. This study showed that the D-test should be used as an obligatory method in routine disk diffusion assay to detect inducible clindamycin resistance in MRSA so that effective antibiotic treatment can be provided.

Citations

Citations to this article as recorded by  
  • Characterization of oxacillin-resistant Staphylococcus lugdunensis isolated from sterile body fluids in a medical center in Taiwan: A 12-year longitudinal epidemiological study
    Shih-Cheng Chang, Jazon Harl Hidrosollo, Lee-Chung Lin, Yu-Hsiang Ou, Cheng-Yen Kao, Jang-Jih Lu
    Journal of Microbiology, Immunology and Infection.2022;[Epub]     CrossRef
  • New update on molecular diversity of clinical Staphylococcus aureus isolates in Iran: antimicrobial resistance, adhesion and virulence factors, biofilm formation and SCCmec typing
    Mahtab Tabandeh, Hami Kaboosi, Mojtaba Taghizadeh Armaki, Abazar Pournajaf, Fatemeh Peyravii Ghadikolaii
    Molecular Biology Reports.2022; 49(4): 3099.     CrossRef
  • Inducible Clindamycin-Resistant Staphylococcus aureus Strains in Africa: A Systematic Review
    Muluneh Assefa, Faham Khamesipour
    International Journal of Microbiology.2022; 2022: 1.     CrossRef
  • Genetic Diversity and Virulence Profile of Methicillin and Inducible Clindamycin-Resistant Staphylococcus aureus Isolates in Western Algeria
    Zahoua Mentfakh Laceb, Seydina M. Diene, Rym Lalaoui, Mabrouk Kihal, Fella Hamaidi Chergui, Jean-Marc Rolain, Linda Hadjadj
    Antibiotics.2022; 11(7): 971.     CrossRef
  • Molecular typing, biofilm formation, and analysis of adhesion factors in Staphylococcus aureus strains isolated from urinary tract infections
    Masoumeh Navidinia, Anis Mohammadi, Reza Arjmand, Masoud Dadashi, Mehdi Goudarzi
    Gene Reports.2021; 22: 101008.     CrossRef
  • Molecular characterization of invasive Staphylococcus aureus strains isolated from patients with diabetes in Iran: USA300 emerges as the major type
    Zahra Tayebi, Maryam Fazeli, Ali Hashemi, Saeed Abdi, Masoud Dadashi, Mohammad Javad Nasiri, Mehdi Goudarzi
    Infection, Genetics and Evolution.2021; 87: 104679.     CrossRef
  • Predominance of PVL-negative community-associated methicillin-resistant Staphylococcus aureus sequence type 8 in newly diagnosed HIV-infected adults, Tanzania
    Joel Manyahi, Sabrina J. Moyo, Said Aboud, Nina Langeland, Bjørn Blomberg
    European Journal of Clinical Microbiology & Infect.2021; 40(7): 1477.     CrossRef
  • Molecular characterization of Staphylococcus aureus strains isolated from hospitalized patients based on coagulase gene polymorphism analysis: High frequency of vancomycin-intermediate S. aureus and the emergence of coagulase type II in Iran
    Malihe Soltani, Bahareh Hajikhani, Samira Zamani, Mehrdad Haghighi, Ali Hashemi, Mohammad Javad Nasiri, Masoud Dadashi, Behzad Pourhossein, Mehdi Goudarzi
    Gene Reports.2021; 23: 101078.     CrossRef
  • Inducible clindamycin resistance among clinical Staphylococcus aureus strains in Iran: A contemporaneous systematic review and meta-analysis
    Mojtaba Memariani, Hamed Memariani, Hamideh Moravvej
    Gene Reports.2021; 23: 101104.     CrossRef
  • Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients
    Amr S. Bishr, Salma M. Abdelaziz, Ibrahim S. Yahia, Mahmoud A. Yassien, Nadia A. Hassouna, Khaled M. Aboshanab
    Biology.2021; 10(7): 624.     CrossRef
  • Relationship between MLSB resistance and the prevalent virulence genotypes among Bulgarian Staphylococcus aureus isolates
    Virna-Maria Tsitou, Ivan Mitov, Raina Gergova
    Acta Microbiologica et Immunologica Hungarica.2021; 68(1): 55.     CrossRef
  • Occurrence, Antibiotic Resistance, Virulence Factors, and Genetic Diversity of Bacillus spp. from Public Hospital Environments in South Africa
    Zamile N. Mbhele, Christiana O. Shobo, Daniel G. Amoako, Oliver T. Zishiri, Linda A. Bester
    Microbial Drug Resistance.2021; 27(12): 1692.     CrossRef
  • Bacterial Antibiotic Resistance: The Most Critical Pathogens
    Giuseppe Mancuso, Angelina Midiri, Elisabetta Gerace, Carmelo Biondo
    Pathogens.2021; 10(10): 1310.     CrossRef
  • Mechanisms of Resistance to Macrolide Antibiotics among Staphylococcus aureus
    Maria Miklasińska-Majdanik
    Antibiotics.2021; 10(11): 1406.     CrossRef
  • Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Human Bloodstream Infections: Detection of MLSB Resistance
    Vanessa Silva, Sara Hermenegildo, Catarina Ferreira, Célia M. Manaia, Rosa Capita, Carlos Alonso-Calleja, Isabel Carvalho, José Eduardo Pereira, Luis Maltez, José L. Capelo, Gilberto Igrejas, Patrícia Poeta
    Antibiotics.2020; 9(7): 375.     CrossRef
  • Genetic analysis of methicillin‐susceptible Staphylococcus aureus clinical isolates: High prevalence of multidrug‐resistant ST239 with strong biofilm‐production ability
    Hossein Goudarzi, Mehdi Goudarzi, Fattaneh Sabzehali, Maryam Fazeli, Alireza Salimi Chirani
    Journal of Clinical Laboratory Analysis.2020;[Epub]     CrossRef
  • Emergence and spread of coagulase type III and staphylococcal cassette chromosome mec type IV among mupirocin-resistant Staphylococcus aureus isolated from wound infections
    Mirmohammad Miri, Maryam Fazeli, Anahita Amirpour, Mohammad Javad Nasiri, Ramin Pouriran, Mehdi Goudarzi
    Gene Reports.2020; 21: 100858.     CrossRef
  • Resistance profile to antimicrobials agents in methicillin-resistant Staphylococcus aureus isolated from hospitals in South Brazil between 2014-2019
    Adriana Medianeira Rossato, Muriel Primon-Barros, Lisiane da Luz Rocha, Keli Cristine Reiter, Cícero Armídio Gomes Dias, Pedro Alves d’Azevedo
    Revista da Sociedade Brasileira de Medicina Tropic.2020;[Epub]     CrossRef
A Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis
Saeed Alamian, Majid Esmaelizad, Taghi Zahraei, Afshar Etemadi, Mohsen Mohammadi, Davoud Afshar, Soheila Ghaderi
Osong Public Health Res Perspect. 2017;8(1):65-70.   Published online February 28, 2017
DOI: https://doi.org/10.24171/j.phrp.2017.8.1.09
  • 3,024 View
  • 52 Download
  • 7 Citations
AbstractAbstract PDF
Objectives

Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis.

Methods

All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available Brucella sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate Brucella abortus from Brucella melitensis. A primer set was designed to flank the unique locus. A total of 136 lymph nodes and blood samples were evaluated and classified by the URS-polymerase chain reaction (PCR) method in 2013–2014.

Results

Biochemical tests and bacteriophage typing as the golden standard indicated that all Brucella spp. isolates were B. melitensis biovar 1 and B. abortus biovar 3. The PCR results were the same as the bacteriological method for detecting Brucella spp. The sensitivity and specificity of the URS-PCR method make it suitable for detecting B. abortus and B. melitensis.

Conclusion

Quick detection of B. abortus and B. melitensis can provide the most effective strategies for control of these bacteria. The advantage of this method over other presented methods is that both B. abortus and B. melitensis are detectable in a single test tube. Furthermore, this method covered 100% of all B. melitensis and B. abortus biotypes. The development of this URS-PCR method is the first step toward the development of a novel kit for the molecular identification of B. abortus and B. melitensis.

Citations

Citations to this article as recorded by  
  • Bovine brucellosis – a comprehensive review
    Sandip Kumar Khurana, Anju Sehrawat, Ruchi Tiwari, Minakshi Prasad, Baldev Gulati, Muhammad Zubair Shabbir, Rajesh Chhabra, Kumaragurubaran Karthik, Shailesh Kumar Patel, Mamta Pathak, Mohd. Iqbal Yatoo, Vivek Kumar Gupta, Kuldeep Dhama, Ranjit Sah, Wanpe
    Veterinary Quarterly.2021; 41(1): 61.     CrossRef
  • Survey of Zoonotic Bacterial Pathogens in Native Foxes in Central Chile: First Record of Brucella canis Exposure
    Nicolás Galarce, Sebastián de la Fuente, Beatriz Escobar, Phillip Dettleff, Pedro Abalos, Juan Carlos Hormazábal, Roberto Flores, Nicole Sallaberry-Pincheira, Víctor Martínez
    Animals.2021; 11(7): 1980.     CrossRef
  • Development and validation of immunoassay for whole cell detection of Brucella abortus and Brucella melitensis
    Richa Hans, Pranjal Kumar Yadav, Pushpendra Kumar Sharma, Mannan Boopathi, Duraipandian Thavaselvam
    Scientific Reports.2020;[Epub]     CrossRef
  • Laboratory Diagnostic Procedures for Human Brucellosis: An Overview of Existing Approaches
    Afshar Etemadi, Rezvan Moniri, Heinrich Neubauer, Yasaman Dasteh Goli, Saeed Alamian
    Jundishapur Journal of Microbiology.2019;[Epub]     CrossRef
  • Comparison of PCR-RFLP and PFGE for determining the clonality of Brucella isolates from human and livestock specimens
    Nasrin Bahmani, Reza Mirnejad, Mohammad Reza Arabestani, Parviz Mohajerie, Seyed Hamid Hashemi, Manoochehr Karami, Mohammad Yousef Alikhani
    Saudi Journal of Biological Sciences.2019; 26(2): 256.     CrossRef
  • Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles
    Arash Shams, Bahareh Rahimian Zarif, Mojtaba Salouti, Reza Shapouri, Sako Mirzaii
    Artificial Cells, Nanomedicine, and Biotechnology.2019; 47(1): 2562.     CrossRef
  • Identification of Brucella genus and eight Brucella species by Luminex bead-based suspension array
    Tina S. Lusk Pfefer, Ruth Timme, Julie A. Kase
    Food Microbiology.2018; 70: 113.     CrossRef
Rapid Molecular Approach for Simultaneous Detection of Salmonella spp., Shigella spp., and Vibrio cholera
Reza Ranjbar, Ali Naghoni, Davoud Afshar, Farhad Nikkhahi, Mohsen Mohammadi
Osong Public Health Res Perspect. 2016;7(6):373-377.   Published online December 31, 2016
DOI: https://doi.org/10.1016/j.phrp.2016.10.002
  • 2,177 View
  • 20 Download
  • 8 Citations
AbstractAbstract PDF
Objectives
Gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including Iran. Early detection of the gastrointestinal tract pathogens can be extremely important. The aim of the current study was to apply a shortened time-multiplex polymerase chain reaction (PCR) for rapid and simultaneous detection of Salmonella spp., Shigella spp., and Vibrio cholera.
Methods
The standard and clinical strains of Salmonella spp., Shigella spp., and V. cholerae were used in the assay. Multiplex PCR was performed and optimized based on amplification of invA, putative integrase, and ompW genes for detecting Salmonella spp., Shigella spp., and V. cholerae, respectively. The specificity of the assay was evaluated by testing 12 different bacterial species.
Results
Only Salmonella spp., Shigella spp., and V. cholerae strains had positive results when subjected to the assay using multiplex PCR. The assay showed a high sensitivity, and no amplification products were observed in multiplex PCR with any of the other microorganisms.
Conclusion
Our study indicated that the invA, putative integrase, and ompW-based multiplex PCR assay appears to be an efficient method for rapid and simultaneous detection of Salmonella spp., Shigella spp., and V. cholerae.

Citations

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  • Rapid and multiplexed quantification of Salmonella, Escherichia coli O157:H7, and Shigella flexneri in ground beef using flow cytometry
    Ziquan Wang, Qian Xu, Siyuan Liu, Yingying Liu, Ying Gao, Meng Wang, Ling Zhang, Haiyan Chang, Qiang Wei, Zhiwei Sui
    Talanta.2022; 238: 123005.     CrossRef
  • Development of multiplex real-time quantitative PCR for simultaneous detection of Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum, and Mycoplasma genitalium in infertile women
    Sara Sadeqi, Farhad Nikkhahi, Amir Javadi, Sonia Eskandarion, Seyed Mahmoud Amin Marashi
    Indian Journal of Medical Microbiology.2022; 40(2): 231.     CrossRef
  • Multiple fluorescent saltatory rolling circle amplification (SRCA) for simultaneous and sensitive detection of Salmonella spp. and Shigella spp. in food
    Wei Guo, Qian Yang, Jie Liu, Xiuling Chen, Yunzhe Zhang, Wei Zhang
    LWT.2022; 168: 113875.     CrossRef
  • Development of rapid gold nanoparticles based lateral flow assays for simultaneous detection of Shigella and Salmonella genera
    Mohammad Lukman Yahaya, Nor Dyana Zakaria, Rahmah Noordin, Khairunisak Abdul Razak
    Biotechnology and Applied Biochemistry.2021; 68(5): 1095.     CrossRef
  • Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor
    Cheryl S.Y. Yeap, Thanyarat Chaibun, Su Yin Lee, Bin Zhao, Yuan Jan, Chan La-o-vorakiat, Werasak Surareungchai, Shiping Song, Benchaporn Lertanantawong
    Chemical Communications.2021; 57(91): 12155.     CrossRef
  • Taqman hydrolysis probe application for Escherichia coli, Salmonella enterica, and Vibrio cholerae detection in surface and drinking water
    Ahmed K. A. El-Sayed, Mohamed I. Abou-Dobara, Camelia A. Abdel-Malak, Amira A. E. El-Badaly
    Journal of Water, Sanitation and Hygiene for Devel.2019; 9(3): 492.     CrossRef
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    Xing Rong Shen, Maomao Xie, Jing Chai, Rui Feng, Jing Cheng, Rong Liu, Paul Kadetz, DeBin Wang
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    Reza Ranjbar, Payam Behzadi, Ali Najafi, Raheleh Roudi
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PHRP : Osong Public Health and Research Perspectives