Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives

OPEN ACCESS
SEARCH
Search

Author index

Page Path
HOME > Articles and issues > Author index
Search
Mohammad Taghi Haghi Ashtiani 1 Article
Virulence Factors of Staphylococcus aureus Isolates in an Iranian Referral Children's Hospital
Farah Sabouni, Shima Mahmoudi, Abbas Bahador, Babak Pourakbari, Reihaneh Hosseinpour Sadeghi, Mohammad Taghi Haghi Ashtiani, Bahram Nikmanesh, Setareh Mamishi
Osong Public Health Res Perspect. 2014;5(2):96-100.   Published online April 30, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.03.002
  • 2,526 View
  • 20 Download
  • 29 Citations
AbstractAbstract PDF
Objectives
The clinical importance of Staphylococcus aureus (S. aureus) is attributed to notable virulence factors, surface proteins, toxins, and enzymes as well as the rapid development of drug resistance. The aim of this study was to compare the occurrence of virulence factors produced by S. aureus strains isolated from children in an Iranian referral children's hospital.
Methods
The presence of genes encoding for the enterotoxins A (sea), B (seb), C (sec), D (sed), TSST-1 (tsst), exfoliative toxin A (eta), and exfoliative toxin B (etb) were detected by Multiplex polymerase chain reaction (PCR) using specific primers. In addition, the standardized Kirby-Bauer disc-diffusion method was performed on Mueller-Hinton agar.
Results
In total, 133 S. aureus isolates were obtained from different patients. Of these S. aureus isolates, 64 (48%) were methicillin-resistant S. aureus (MRSA), and all of these tested positive for the mecA gene. Regarding the classical enterotoxin genes, sea gene (40.6%) was the most prevalent followed by seb (19.6%), tsst (12.8%), eta (11.3%), etb (9%), sed (4.5%), and sec (3%). Among methicillin-susceptible S. aureus (MSSA) isolates, seb and tsst were the more prevalent toxins in comparison with MRSA isolates (p < 0.05), while the frequency of sea, sed, eta, and etb genes were higher among MRSA isolates (p > 0.05).
Conclusion
In our study enterotoxin A was produced by 40.6% of the isolates (48% from MRSA and 33% from MSSA isolates) which was higher than in previous reports. According to our results, strict hygiene and preventative measures during food processing are highly recommended.

Citations

Citations to this article as recorded by  
  • New update on molecular diversity of clinical Staphylococcus aureus isolates in Iran: antimicrobial resistance, adhesion and virulence factors, biofilm formation and SCCmec typing
    Mahtab Tabandeh, Hami Kaboosi, Mojtaba Taghizadeh Armaki, Abazar Pournajaf, Fatemeh Peyravii Ghadikolaii
    Molecular Biology Reports.2022; 49(4): 3099.     CrossRef
  • Investigation of Virulence Genes of the Predominant Bacteria Associated with Renal Stones and their Correlation with Postoperative Septic Complications
    Asmaa E Ahmed, Hassan Abol-Enein, Amira Awadalla, Heba El degla, Omar A El-Shehaby
    Infection and Drug Resistance.2022; Volume 15: 3643.     CrossRef
  • Virulence genes distributed among Staphylococcus aureus causing wound infections and their correlation to antibiotic resistance
    Asia Helmi Rasmi, Eman Farouk Ahmed, Abdou Mohammed Abdullah Darwish, Gamal Fadl Mahmoud Gad
    BMC Infectious Diseases.2022;[Epub]     CrossRef
  • Genetic Diversity and Virulence Profile of Methicillin and Inducible Clindamycin-Resistant Staphylococcus aureus Isolates in Western Algeria
    Zahoua Mentfakh Laceb, Seydina M. Diene, Rym Lalaoui, Mabrouk Kihal, Fella Hamaidi Chergui, Jean-Marc Rolain, Linda Hadjadj
    Antibiotics.2022; 11(7): 971.     CrossRef
  • Antimicrobial susceptibility and genotyping of microorganisms isolated from sputum culture of children with cystic fibrosis in an Iranian referral children’s hospital
    Setareh Mamishi, Arezu Akhlaghi, Babak Pourakbari, Mohammadreza Modaresi, Mohammad Taghi Haghi Ashtiani, Reihaneh Hosseinpour Sadeghi, Rohola Shirzadi, Zohreh Shalchi, Shima Mahmoudi
    Wiener Medizinische Wochenschrift.2022;[Epub]     CrossRef
  • Phenotypic and genotypic virulence features of staphylococcal strains isolated from difficult-to-treat skin and soft tissue infections
    Mădălina Preda, Mara Mădălina Mihai, Laura Ioana Popa, Lia-Mara Dițu, Alina Maria Holban, Loredana Sabina Cornelia Manolescu, Gabriela-Loredana Popa, Andrei-Alexandru Muntean, Irina Gheorghe, Carmen Mariana Chifiriuc, Mircea-Ioan Popa, Keun Seok Seo
    PLOS ONE.2021; 16(2): e0246478.     CrossRef
  • Distribution of virulence genes and SCCmec types among methicillin-resistant Staphylococcus aureus of clinical and environmental origin: a study from community of Assam, India
    Deepshikha Bhowmik, Shiela Chetri, Bhaskar Jyoti Das, Debadatta Dhar Chanda, Amitabha Bhattacharjee
    BMC Research Notes.2021;[Epub]     CrossRef
  • The anti-biofilm capability of nano-emodin-mediated sonodynamic therapy on multi-species biofilms produced by burn wound bacterial strains
    Maryam Pourhajibagher, Bahman Rahimi-esboei, Hanie Ahmadi, Abbas Bahador
    Photodiagnosis and Photodynamic Therapy.2021; 34: 102288.     CrossRef
  • Prevalence of methicillin-resistant Staphylococcus aureus in Iranian children: a systematic review and meta-analysis
    Farhad Sarrafzadeh, Seyed Mojtaba Sohrevardi, Hamid Abousaidi, Hossein Mirzaei
    Clinical and Experimental Pediatrics.2021; 64(8): 415.     CrossRef
  • Interactions between staphylococcal enterotoxins A and D and superantigen-like proteins 1 and 5 for predicting methicillin and multidrug resistance profiles among Staphylococcus aureus ocular isolates
    Min Lu, Jean-Marie Parel, Darlene Miller, Abdelazeem Mohamed Algammal
    PLOS ONE.2021; 16(7): e0254519.     CrossRef
  • Four Weeks Exercise in Vary Intensities Reduce More Inguinal Fat Than Perigonadal Fat in Mice
    Ahmad Rukhani Lutfi, Paulus Liben, Lilik Herawati
    Folia Medica Indonesiana.2021; 57(2): 121.     CrossRef
  • Molecular Genotyping of Tsst-1 Gene Staphylococcus Aureus from Clinical Specimen
    Marinda Dwi Puspitarini, Kuntaman Kuntaman, Eddy Bagus Wasito, Wahyu Setyarini
    Folia Medica Indonesiana.2021; 57(2): 129.     CrossRef
  • Prevalence of enterotoxin genes (SEA to SEE) and antibacterial resistant pattern of Staphylococcus aureus isolated from clinical specimens in Assiut city of Egypt
    Ahmed A. Baz, Elsayed K. Bakhiet, Usama Abdul-Raouf, Ahmed Abdelkhalek
    Egyptian Journal of Medical Human Genetics.2021;[Epub]     CrossRef
  • Contemporary systematic review and meta-analysis of exfoliative toxin-producing Staphylococcus aureus strains isolated from patients in Iran
    Mojtaba Memariani, Hamed Memariani, Mohammad Shahidi-Dadras, Zohreh Tehranchinia, Fariba Ghalamkarpour, Hamideh Moravvej
    Reviews in Medical Microbiology.2020; 31(1): 1.     CrossRef
  • Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells
    Inés Reigada, Ramón Pérez-Tanoira, Jayendra Z. Patel, Kirsi Savijoki, Jari Yli-Kauhaluoma, Teemu J. Kinnari, Adyary Fallarero
    Microorganisms.2020; 8(3): 345.     CrossRef
  • Characterization of different virulent factors in methicillin-resistant Staphylococcus aureus isolates recovered from Iraqis and Syrian refugees in Duhok city, Iraq
    Narin A. Rasheed, Nawfal R. Hussein, Herminia de Lencastre
    PLOS ONE.2020; 15(8): e0237714.     CrossRef
  • Inhibition of growth and gene expression in Staphylococcus aureus by anti-gyrA peptide nucleic acid
    Mahnaz Tavakoli, Ali Hashemi, Maryam Vaezjalali, Mohammad Mohammadzadeh, Hossein Goudarzi
    Future Microbiology.2019; 14(13): 1123.     CrossRef
  • Quorum Sensing: A Prospective Therapeutic Target for Bacterial Diseases
    Qian Jiang, Jiashun Chen, Chengbo Yang, Yulong Yin, Kang Yao
    BioMed Research International.2019; 2019: 1.     CrossRef
  • Survey of Staphylococcus aureus in a general pediatric population and focus on isolates with three clinically relevant toxin-encoding genes
    Anne Filleron, Sarah Beauregard-Birba, Thibault Mura, Fabien Aujoulat, Anne Laure Michon, Michel Rodière, Tu Anh Tran, Eric Jeziorski, Hélène Marchandin
    World Journal of Pediatrics.2018; 14(1): 35.     CrossRef
  • Characterization of Virulence Factors and Prophage Profiles of Methicillin-Resistant Staphylococcus aureus Strains Isolated from a Referral Hospital in Tehran, Iran
    Fateh Rahimi, Leili Shokoohizadeh
    Archives of Clinical Infectious Diseases.2018;[Epub]     CrossRef
  • Inhibitory Effects of Curcumin on the Expression of NorA Efflux Pump and Reduce Antibiotic Resistance in Staphylococcus aureus
    Samin Jaberi, Fateme Fallah, Ali Hashemi, Ahmad Karimi, Leila Azimi
    Journal of Pure and Applied Microbiology.2018; 12(1): 95.     CrossRef
  • Relationships among superantigen toxin gene profiles, genotypes, and pathogenic characteristics of Staphylococcus aureus isolates from bovine mastitis
    Dong Wang, Limei Zhang, Changfu Yong, Mingliang Shen, Tariq Ali, Muhammad Shahid, Kun Han, Xuezhang Zhou, Bo Han
    Journal of Dairy Science.2017; 100(6): 4276.     CrossRef
  • Molecular epidemiology of Staphylococcus aureus nasal colonization among patients and their parents /guardian in an Iranian referral hospital
    Babak Pourakbari, Mahmoud Khodabandeh, Shima Mahmoudi, Farah Sabouni, Alireza Aziz-Ahari, Abbas Bahador, Sepideh Keshavarz Valian, Reihaneh Hosseinpour Sadeghi, Setareh Mamishi
    Microbial Pathogenesis.2017; 107: 75.     CrossRef
  • The effect of antimicrobial photodynamic therapy on the expression of novel methicillin resistance markers determined using cDNA-AFLP approach in Staphylococcus aureus
    Mohammad Neshvan Hoorijani, Hosein Rostami, Maryam Pourhajibagher, Nasim Chiniforush, Mansour Heidari, Babak Pourakbari, Hossein Kazemian, Kambiz Davari, Vahid Amini, Reza Raoofian, Abbas Bahador
    Photodiagnosis and Photodynamic Therapy.2017; 19: 249.     CrossRef
  • Antimicrobial susceptibility of microorganisms isolated from sputum culture of patients with cystic fibrosis: Methicillin-resistant Staphylococcus aureus as a serious concern
    Narges Mazloomi Nobandegani, Shima Mahmoudi, Babak Pourakbari, Reihaneh Hosseinpour Sadeghi, Mehri Najafi Sani, Fateme Farahmand, Farzaneh Motamed, Raheleh Nabavizadeh Rafsanjani, Setareh Mamishi
    Microbial Pathogenesis.2016; 100: 201.     CrossRef
  • The Correlation between Toxin Genotype and Antibiotic Resistance in Methicillin ResistantStaphylococcus aureusIsolated from Clinical Specimen of Intensive Care Unit
    Chul Park, Chi Nam Seong
    Korean Journal of Clinical Laboratory Science.2016; 48(3): 202.     CrossRef
  • Studies on Enterotoxins and Antimicrobial Resistance in <i>Staphylococcus aureus</i> Isolated from Various Sources
    Enas Yasser Mohammed, Shaymaa Hassan Abdel-Rhman, Rasha Barwa, Mohammed Adel El-Sokkary
    Advances in Microbiology.2016; 06(04): 263.     CrossRef
  • Genotype Analyses of Methicillin Resistant Staphylococcus aureus Isolated from clinical specimens
    Jean-Soo Kim, Chul Park
    Journal of the Korea Academia-Industrial cooperati.2015; 16(5): 3315.     CrossRef
  • Frequency of the toxic shock syndrome toxin-1 gene in methicillin-susceptible and -resistant Staphylococcus aureus isolates from teaching hospitals in Shiraz, Iran
    Mohammad Motamedifar, Hadi Sedigh Ebrahim-Saraie, Seyedeh Mahsan Hoseini Alfatemi, Mehrdad Zalipour, Mohammad Kaveh, Hossein Khoshkharam-Roodmajani
    Revista da Sociedade Brasileira de Medicina Tropic.2015; 48(1): 90.     CrossRef

PHRP : Osong Public Health and Research Perspectives