- Phenotypic Assays to Determine Virulence Factors of Uropathogenic Escherichia coli (UPEC) Isolates and their Correlation with Antibiotic Resistance Pattern
-
Mohsen Tabasi, Mohammad Reza Asadi Karam, Mehri Habibi, Mir Saeed Yekaninejad, Saeid Bouzari
-
Osong Public Health Res Perspect. 2015;6(4):261-268. Published online August 31, 2015
-
DOI: https://doi.org/10.1016/j.phrp.2015.08.002
-
-
2,277
View
-
18
Download
-
39
Citations
-
 Abstract
 PDF
- Objectives
Urinary tract infection caused by uropathogenic Escherichia coli (UPEC) strains is one of the most important infections in the world. UPEC encode widespread virulence factors closely related with pathogenesis of the bacteria. The purpose of this study was to evaluate the presence of different phenotypic virulence markers in UPEC isolates and determine their correlation with antibiotic resistance pattern.
Methods
UPEC isolates from patients with different clinical symptoms of UTI were collected and screened for biofilm and hemolysin production, mannose resistant, and mannose sensitive hemagglutination (MRHA and MSHA, respectively). In addition, antimicrobial resistance pattern and ESBL-producing isolates were recorded.
Results
Of the 156 UPEC isolates, biofilm and hemolysin formation was seen in 133 (85.3%) and 53 (34%) isolates, respectively. Moreover, 98 (62.8%) and 58 (37.2%) isolates showed the presence of Types 1 fimbriae (MSHA) and P fimbriae (MRHA), respectively. Our results also showed a relationship between biofilm formation in UPEC isolated from acute cystitis patients and recurrent UTI cases. Occurrence of UTI was dramatically correlated with the patients' profiles. We observed that the difference in antimicrobial susceptibilities of the biofilm and nonbiofilm former isolates was statistically significant. The UPEC isolates showed the highest resistance to ampicillin, tetracycline, amoxicillin, and cotrimoxazole. Moreover, 26.9% of isolates were ESBL producers.
Conclusion
This study indicated that there is a relationship between the phenotypic virulence traits of the UPEC isolates, patients' profiles, and antibiotic resistance. Detection of the phenotypic virulence factors could help to improve understanding of pathogenesis of UPEC isolates and better medical intervention.
-
Citations
Citations to this article as recorded by  - Detection of Adhesion Encoding Genes, Antibacterial Susceptibility Test and Biofilm Formation of Uropathogenic Escherichia coli Isolated from Urinary Tract Infections in Children
Rezvan Goodarzi, Rasoul Yousefimashouf, Iraj Sedighi, Abbas Moradi, Fatemeh Nouri, Mohammad Taheri
Journal of Advances in Medical and Biomedical Rese.2022; 30(1): 1. CrossRef - A global systematic review and meta-analysis on correlation between biofilm producers and non-biofilm producers with antibiotic resistance in Uropathogenic Escherichia coli
Mitra Garousi, Sina Monazami Tabar, Hosein Mirazi, Parnia Asgari, Paniz Sabeghi, Astireh Salehi, Azad Khaledi, Mohammad Ghenaat Pisheh Sanani, Hossein Karballaei Mirzahosseini
Microbial Pathogenesis.2022; 164: 105412. CrossRef - Antibiotic resistance, phylogenetic typing, and virulence genes profile analysis of uropathogenic Escherichia coli isolated from patients in southern Iraq
Mohammed Allami, Masoumeh Bahreini, Mohammad Reza Sharifmoghadam
Journal of Applied Genetics.2022; 63(2): 401. CrossRef - Phylogenetic Group Distribution of Uropathogenic Escherichia coli and Related Antimicrobial Resistance Pattern: A Meta-Analysis and Systematic Review
Mehrdad Halaji, Amirhossein Fayyazi, Mehdi Rajabnia, Donya Zare, Abazar Pournajaf, Reza Ranjbar
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Genotypic assay to determine some virulence factors of Uropathogenic E. coli (UPEC) isolates
Tsahel H. Al-Dulaimi, Ilham A Bunyan, Thikra A. Banimuslem
International journal of health sciences.2022; : 1593. CrossRef - Demonstrating the utility of Escherichia coli asymptomatic bacteriuria isolates’ virulence profile towards diagnosis and management—A preliminary analysis
Lalitha Maniam, Kumutha Malar Vellasamy, Hassan Mahmood Jindal, Vallikannu Narayanan, Mahmoud Danaee, Jamuna Vadivelu, Vinod Pallath, Abdelazeem Mohamed Algammal
PLOS ONE.2022; 17(5): e0267296. CrossRef - Urine Microscopy Score and Neutrophil Lymphocyte Ratio at Presentation are Good Biomarkers of Acute Kidney Injury in Patients with Upper Urinary Tract Infection when Assessed in Correlation with Virulence Factors of Escherichia coli and Blood Group Secret
Shanmugapriya Thiagarajan, Selvaraj Stephen, Santosh Kumar, Priscilla Charles, Sarangapani Kanagamuthu, Stanley Ambroise, Pragasam Viswanathan, Palanivel Chinnakali, Rajesh Nachiappa Ganesh
Journal of Pure and Applied Microbiology.2022; 16(2): 1074. CrossRef - Association between Virulence Factors and Antimicrobial Resistance of Klebsiella pneumoniae Clinical Isolates from North Kerala
Ramya Kumaran, R.V. Geetha, Sabitha Baby
Journal of Pure and Applied Microbiology.2022; 16(2): 867. CrossRef - Molecular Characterization and Mutational Analysis of Fluoroquinolones and Tetracycline Resistant Genes of Escherichia coli Isolated from UTI Patients
Sadiq Azam, Nauman Khan, Noor Rehman, Ibrar khan, Amjad Ali, Muhammad Asghar, Azam Hayat, Gulesehra Mujib, Anila Farid
Brazilian Archives of Biology and Technology.2022;[Epub] CrossRef - Virulence genes and phylogenetic groups of uropathogenic Escherichia coli isolates from patients with urinary tract infection and uninfected control subjects: a case-control study
Seyedeh Elham Rezatofighi, Mahsa Mirzarazi, Mansour Salehi
BMC Infectious Diseases.2021;[Epub] CrossRef - Virulence factors and antimicrobial resistance of uropathogenic Escherichia coli (UPEC) isolated from urinary tract infections: a systematic review and meta-analysis
Gabriel Kambale Bunduki, Eva Heinz, Vincent Samuel Phiri, Patrick Noah, Nicholas Feasey, Janelisa Musaya
BMC Infectious Diseases.2021;[Epub] CrossRef - Evaluation of Biofilm Formation and Virulence Genes and Association with Antibiotic Resistance Patterns of Uropathogenic Escherichia coli Strains in Southwestern Iran
Mostafa Boroumand, Asghar Sharifi, Mohammad Amin Ghatei, Mohsen Sadrinasab
Jundishapur Journal of Microbiology.2021;[Epub] CrossRef - The urobiome, urinary tract infections, and the need for alternative therapeutics
Jennifer Jones, Craig P. Murphy, Roy D. Sleator, Eamonn P. Culligan
Microbial Pathogenesis.2021; 161: 105295. CrossRef - Clinical cases, drug resistance, and virulence genes profiling in Uropathogenic Escherichia coli
Ali Hozzari, Payam Behzadi, Parisa Kerishchi Khiabani, Mohammad Sholeh, Niloofar Sabokroo
Journal of Applied Genetics.2020; 61(2): 265. CrossRef Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic E. coli Isolated from Egyptian Patients with Different Clinical Infections
Rehab Mahmoud Abd El-Baky, Reham Ali Ibrahim, Doaa Safwat Mohamed, Eman Farouk Ahmed, Zeinab Shawky Hashem
Infection and Drug Resistance.2020; Volume 13: 1221. CrossRefIn-vitro Investigation of Antibiotics Efficacy Against Uropathogenic Escherichia coli Biofilms and Antibiotic Induced Biofilm Formation at Sub-Minimum Inhibitory Concentration of Ciprofloxacin
Zara Rafaque, Nasira Abid, Nida Liaquat, Pashmina Afridi, Saima Siddique, Safia Masood, Sehrish Kanwal, Javid Iqbal Dasti
Infection and Drug Resistance.2020; Volume 13: 2801. CrossRef- Relationship between Virulence and Resistance among Gram-Negative Bacteria
Virginio Cepas, Sara M. Soto
Antibiotics.2020; 9(10): 719. CrossRef - In Vitro and In Vivo Biological Activity of Berberine Chloride against Uropathogenic E. coli Strains Using Galleria mellonella as a Host Model
Giulio Petronio Petronio, Marco Alfio Cutuli, Irene Magnifico, Noemi Venditti, Laura Pietrangelo, Franca Vergalito, Antonella Pane, Giovanni Scapagnini, Roberto Di Marco
Molecules.2020; 25(21): 5010. CrossRef - Study of virulence factors and antibiotic susceptibility pattern of extraintestinal pathogenic Escherichia coli
Mahendraswamy B Hiremath, R Lava
Indian Journal of Microbiology Research.2020; 7(4): 330. CrossRef - Virulence factors of uropathogenic Escherichia coli (UPEC) and correlation with antimicrobial resistance
Chhaya Shah, Ratna Baral, Bijay Bartaula, Lok Bahadur Shrestha
BMC Microbiology.2019;[Epub] CrossRef - Outcome of acute urinary tract infections caused by uropathogenic Escherichia coli with phenotypically demonstrable virulence factors
Unnimaya Pullanhi, Sadia Khan, Vivek Vinod, Karthika Mohan, Anil Kumar
Annals of African Medicine.2019; 18(3): 138. CrossRef - Urinary tract infection: Pathogenicity, antibiotic resistance and development of effective vaccines against Uropathogenic Escherichia coli
Mohammad Reza Asadi Karam, Mehri Habibi, Saeid Bouzari
Molecular Immunology.2019; 108: 56. CrossRef - Systematic analysis of research on D-mannose and the prospects for its use in recurrent infections of the urinary tract in women of reproductive age
O. A. Gromova, I. Yu. Torshin, N. K. Tetruashvili
Obstetrics, Gynecology and Reproduction.2019; 13(2): 119. CrossRef - Evaluation of pap and sfa Genes Relative Frequency P and S Fimbriae Encoding of Uropathogenic Escherichia coli Isolated from Hospitals and Medical Laboratories; Yasuj City, Southwest Iran
Mostafa Boroumand, Asghar Sharifi, Leila Manzouri, Seyed Sajjad Khoramrooz, Seyed Abdolmajid Khosravani
Iranian Red Crescent Medical Journal.2019;[Epub] CrossRef - Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal
Raju Shrestha, Santosh Khanal, Pramod Poudel, Karan Khadayat, Sajani Ghaju, Anita Bhandari, Sunil Lekhak, Narayan Dutt Pant, Manisha Sharma, Bishnu P. Marasini
Annals of Clinical Microbiology and Antimicrobials.2019;[Epub] CrossRef - Genotyping and molecular characterization of extended-spectrum beta-lactamases-producing uropathogenic Escherichia coli in and around Coimbatore district, Tamil Nadu, India
Mylsamy Muraleetharan, Thirumoorthy Viswanathan
Urological Science.2019; 30(6): 244. CrossRef - Investigation of P Fimbriae Presence in Escherichia coli Strains Isolated from Urine Samples in Human, and Their Antibacterial Resistance
Emel Inegol Paykoc, Suheyla Turkyilmaz
Jundishapur Journal of Microbiology.2018;[Epub] CrossRef - An evaluation of multidrug-resistant Escherichia coli isolates in urinary tract infections from Aguascalientes, Mexico: cross-sectional study
Flor Y. Ramírez-Castillo, Adriana C. Moreno-Flores, Francisco J. Avelar-González, Francisco Márquez-Díaz, Josée Harel, Alma L. Guerrero-Barrera
Annals of Clinical Microbiology and Antimicrobials.2018;[Epub] CrossRef - Potential Mechanisms Underlying the Increased Excitability of the Bladder Afferent Pathways in Interstitial Cystitis/Bladder Pain Syndrome
Doo Sang Kim
Urogenital Tract Infection.2018; 13(2): 26. CrossRef - Distribution of virulence genes and their association with antimicrobial resistance among uropathogenic Escherichia coli isolates from Iranian patients
Yalda Malekzadegan, Reza Khashei, Hadi Sedigh Ebrahim-Saraie, Zahra Jahanabadi
BMC Infectious Diseases.2018;[Epub] CrossRef - Important Virulence Factors and Related Genes in Uropathogenic E. coli and their Relation to Fluoroquinolone Resistance
Noha Mohammad Gohar, Hanaa Fathy Aly, Magda Ibrahim Ayoub
Journal of Pure and Applied Microbiology.2018; 12(3): 1393. CrossRef - Clonal and Virulence Distribution of UropathogenicEscherichia coliIsolated from Children in Korea
Dong Ho Kim, Chul Hee Choi
Journal of Bacteriology and Virology.2017; 47(1): 54. CrossRef - An Epidemiological Study on the Prevalence and Antibiotic Resistance Patterns of Bacteria Isolated from Urinary Tract Infections in Central Iran
Fahimeh Ghanbari, Farzad Khademi, Shirin Saberianpour, Mojtaba Shahin, Nafiseh Ghanbari, Kourosh Naderi, Tahereh Motalebi-Rad
Avicenna Journal of Clinical Microbiology and Infe.2017; 4(3): 42214. CrossRef - Correlation Between hlyA and cnf1 Virulent Genes with Antibiotic Resistance and non-ESBLs Escherichia coli Isolates Collected from Patient with Urinary Tract Infections in Kerman, Iran
Zahra Hashemizadeh, Davood Kalantar-Neyestanaki, Shahla Mansouri
Archives of Pediatric Infectious Diseases.2017;[Epub] CrossRef - Prevalence and Antibiotic Resistance Profile of Enterobacteria Involved in Urinary Infections in Bamako, Mali
Amadou Hamadoun Babana
MOJ Biology and Medicine.2017;[Epub] CrossRef - Phenotypic and Molecular Characterization of some Virulence Factors in Multidrug Resistance Escherichia coli Isolated from Different Clinical Infections in Iraq
Ahmed Abduljabbar Ja Aljanaby, Qassim Mmuhsin Hashi Alfaham
American Journal of Biochemistry and Molecular Bio.2017; 7(2): 65. CrossRef - Emerging nanotechnology based strategies for diagnosis and therapeutics of urinary tract infections: A review
M.S. Kumar, A.P. Das
Advances in Colloid and Interface Science.2017; 249: 53. CrossRef - Effects of single and combined use of bacteriophages and antibiotics to inactivate Escherichia coli
Nádia Valério, Cristiana Oliveira, Vânia Jesus, Tatiana Branco, Carla Pereira, Catarina Moreirinha, Adelaide Almeida
Virus Research.2017; 240: 8. CrossRef -
Crystal Structures of Acyclic Nucleoside Phosphonates in Complex with
Escherichia coli
Hypoxanthine Phosphoribosyltransferase
Wai Soon Eng, Dana Hocková, Petr Špaček, Ondřej Baszczyňski, Zlatko Janeba, Lieve Naesens, Dianne T. Keough, Luke W. Guddat
ChemistrySelect.2016; 1(19): 6267. CrossRef
|
