- Association between Beta-lactam Antibiotic Resistance and Virulence Factors in AmpC Producing Clinical Strains of P. aeruginosa
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Sanaz Dehbashi, Hamed Tahmasebi, Mohammad Reza Arabestani
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Osong Public Health Res Perspect. 2018;9(6):325-333. Published online December 31, 2018
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DOI: https://doi.org/10.24171/j.phrp.2018.9.6.06
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 Abstract
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Objectives
The purpose of this study was to determine the presence of IMP and OXA genes in clinical strains of Pseudomonas aeruginosa (P. aeruginosa) that are carriers of the ampC gene.
Methods
In this study, 105 clinical isolates of P. aeruginosa were collected. Antibiotic resistance patterns were determined using the disk diffusion method. The strains carrying AmpC enzymes were characterized by a combination disk method. Multiplex-PCR was used to identify resistance and virulence genes, chi-square test was used to determine the relationship between variables.
Results
Among 105 isolates of P. aeruginosa, the highest antibiotic resistance was to cefotaxime and aztreonam, and the least resistance was to colictin and ceftazidime. There were 49 isolates (46.66%) that showed an AmpC phenotype. In addition, the frequencies of the resistance genes were; OXA48 gene 85.2%, OXA199, 139 3.8%, OXA23 3.8%, OXA2 66.6%, OXA10 3.8%, OXA51 85.2% and OXA58 3.8%. The IMP27 gene was detected in 9 isolates (8.57%) and the IMP3.34 was detected in 11 isolates (10.47%). Other genes detected included; lasR (17.1%), lasB (18%) and lasA (26.6%). There was a significant relationship between virulence factors and the OX and IMP genes (p ≤ 0.05).
Conclusion
The relationship between antibiotic resistance and virulence factors observed in this study could play an important role in outbreaks associated with P. aeruginosa infections.
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Citations
Citations to this article as recorded by  - Prevalence -lactam resistant Pseudomonas aeruginosa strains isolated from chronic supportive otitis media infections: A single center survey in Eastern India
Shakti Rath, Debidatta Singhsamanta, Debasmita Dubey, Saumya Ranjan Das
IP International Journal of Medical Microbiology a.2024; 10(3): 258. CrossRef - Prevalence of colistin resistance in clinical isolates of Pseudomonas aeruginosa: a systematic review and meta-analysis
Negar Narimisa, Abbasali Keshtkar, Leila Dadgar-Zankbar, Narjess Bostanghadiri, Yasaman Rouein Far, Soheila Shahroodian, Abed Zahedi Bialvaei, Shabnam Razavi
Frontiers in Microbiology.2024;[Epub] CrossRef - Comparative bacteriome and antibiotic resistome analysis of water and sediment of the Ganga River of India
Ankita Srivastava, Digvijay Verma
World Journal of Microbiology and Biotechnology.2023;[Epub] CrossRef - Prevalence of different virulence factors and their association with antimicrobial resistance among Pseudomonas aeruginosa clinical isolates from Egypt
Eva A. Edward, Marwa R. El Shehawy, Alaa Abouelfetouh, Elsayed Aboulmagd
BMC Microbiology.2023;[Epub] CrossRef - Molecular epidemiology and collaboration of siderophore-based iron acquisition with surface adhesion in hypervirulent Pseudomonas aeruginosa isolates from wound infections
Hamed Tahmasebi, Sanaz Dehbashi, Mona Nasaj, Mohammad Reza Arabestani
Scientific Reports.2022;[Epub] CrossRef - Decoding Genetic Features and Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains Isolated from Bloodstream Infections
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Agnieszka Mikucka
International Journal of Molecular Sciences.2022; 23(16): 9208. CrossRef - Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the Pseudomonas aeruginosa Clinical Strains
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecińska-Piróg, Eugenia Gospodarek-Komkowska
Antibiotics.2021; 10(3): 241. CrossRef - A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii
Mahyar Porbaran, Hamed Tahmasebi, MohammadReza Arabestani, Joseph Falkinham
International Journal of Microbiology.2021; 2021: 1. CrossRef - Regulation of virulence and β-lactamase gene expression in Staphylococcus aureus isolates: cooperation of two-component systems in bloodstream superbugs
Sanaz Dehbashi, Hamed Tahmasebi, Behrouz Zeyni, Mohammad Reza Arabestani
BMC Microbiology.2021;[Epub] CrossRef -
New approach to identify colistin‐resistant
Pseudomonas aeruginosa
by high‐resolution melting curve analysis assay
H. Tahmasebi, S. Dehbashi, M.R. Arabestani
Letters in Applied Microbiology.2020; 70(4): 290. CrossRef - Resistant Pseudomonas aeruginosa carrying virulence genes in hospitalized patients with urinary tract infection from Sanandaj, west of Iran
Safoura Derakhshan, Aslan Hosseinzadeh
Gene Reports.2020; 20: 100675. CrossRef - Prevalence and molecular typing of Metallo-β-lactamase-producing Pseudomonas aeruginosa with adhesion factors: A descriptive analysis of burn wounds isolates from Iran
Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Yousef Alikhani, Mahyar Porbaran, Mohammad Reza Arabestani
Gene Reports.2020; 21: 100853. CrossRef - Co-harboring of mcr-1 and β-lactamase genes in Pseudomonas aeruginosa by high-resolution melting curve analysis (HRMA): Molecular typing of superbug strains in bloodstream infections (BSI)
Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Reza Arabestani
Infection, Genetics and Evolution.2020; 85: 104518. CrossRef - Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran
Mahyar Porbaran, Reza Habibipour
Journal of Pure and Applied Microbiology.2020; 14(3): 1723. CrossRef - Carbapenem-Resistant Pseudomonas aeruginosa Strains-Distribution of the Essential Enzymatic Virulence Factors Genes
Tomasz Bogiel, Małgorzata Prażyńska, Joanna Kwiecińska-Piróg, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska
Antibiotics.2020; 10(1): 8. CrossRef - Biofilm Formation and β-lactamase Enzymes: A Synergism Activity in Acinetobacter baumannii Isolated from Wound Infection
Mahyar Porbaran, Reza Habibipour
Journal of Advances in Medical and Biomedical Rese.2019; 27(125): 34. CrossRef
- Detection of Integrons and Staphylococcal Cassette Chromosome mec Types in Clinical Methicillin-resistant Coagulase Negative Staphylococci Strains
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Fahimeh Hajiahmadi, Elham Salimi Ghale, Mohammad Yousef Alikhani, Alireza Mordadi, Mohammad Reza Arabestani
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Osong Public Health Res Perspect. 2017;8(1):47-53. Published online February 28, 2017
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DOI: https://doi.org/10.24171/j.phrp.2017.8.1.06
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6,156
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41
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Abstract
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- Objectives
Integrons are thought to play an important role in the spread of antibiotic resistance. This study investigates class 1 and 2 integron-positive methicillin-resistant coagulase-negative staphylococci strains isolated in Iran and characterizes their patterns of antimicrobial resistance. MethodsHundred clinical isolates of coagulase-negative staphylococci were characterized for integron content and staphylococcal cassette chromosome mec (SCCmec) type. ResultsSixteen isolates carried class 1 (intI1) integrons and four isolates carried class 2 (intI2) integrons. One resistance gene array was identified among the class 1 integrons (aadA1 cassette). The distribution of SCCmec types in 50 methicillin-resistant coagulase-negative staphylococci strains showed that SCCmec types III and V dominated among the tested strains. ConclusionThis is the first report of methicillin-resistant coagulase-negative staphylococci strains that carry two mobile genetic elements, including class 1 and 2 integrons and SCCmec, in Iran.
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Citations
Citations to this article as recorded by - Prevalence and Potential Risk Factors for the Acquisition of Antibiotic‐Resistant Staphylococcus spp. Bacteria Among Pastoralist Farmers in Kajiado Central Subcounty, Kenya
Edidah Ong’era, John Kagira, Naomi Maina, Daniel Kiboi, Kenneth Waititu, Lynda Michira, Maina Ngotho, Mejdi Snoussi
BioMed Research International.2023;[Epub] CrossRef - Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran
Effat Abbasi Montazeri, Sakineh Seyed-Mohammadi, Aram Asarehzadegan Dezfuli, Azar Dokht Khosravi, Maryam Dastoorpoor, Mitra Roointan, Morteza Saki
Bioscience Reports.2020;[Epub] CrossRef
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