- Analysis of Resistance to Macrolide–Lincosamide–Streptogramin B Among mecA-Positive Staphylococcus Aureus Isolates
-
Mahmoud Khodabandeh, Mohsen Mohammadi, Mohammad Reza Abdolsalehi, Azadeh Alvandimanesh, Mehrdad Gholami, Meysam Hasannejad Bibalan, Abazar Pournajaf, Ramin Kafshgari, Ramazan Rajabnia
-
Osong Public Health Res Perspect. 2019;10(1):25-31. Published online February 28, 2019
-
DOI: https://doi.org/10.24171/j.phrp.2019.10.1.06
-
-
4,199
View
-
172
Download
-
21
Citations
-
 Abstract
 PDF
-
Objectives
Genetic determinants conferring resistance to macrolide, lincosamide, and streptogramin B (MLSB) via ribosomal modification such as, erm, msrA/B and ereA/B genes are distributed in bacteria. The main goals of this work were to evaluate the dissemination of MLSB resistance phenotypes and genotypes in methicillin-resistant Staphylococcus aureus (MRSA) isolates collected from clinical samples.
Methods
A total of 106 MRSA isolates were studied. Isolates were recovered from 3 hospitals in Tehran between May 2016 to July 2017. The prevalence of MLSB-resistant strains were determined by D-test, and then M-PCR was performed to identify genes encoding resistance to macrolides, lincosamides, and streptogramins in the tested isolates.
Results
The frequency of constitutive resistance MLSB, inducible resistance MLSB and MSB resistance were 56.2%, 22.9%, and 16.6%, respectively. Of 11 isolates with the inducible resistance MLSB phenotype, ermC, ermB, ermA and ereA were positive in 81.8%, 63.6%, 54.5% and 18.2% of these isolates, respectively. In isolates with the constitutive resistance MLSB phenotype, the prevalence of ermA, ermB, ermC, msrA, msrB, ereA and ereB were 25.9%, 18.5%, 44.4%, 0.0%, 0.0%, 11.1% and 0.0%, respectively.
Conclusion
Clindamycin is commonly administered in severe MRSA infections depending upon the antimicrobial susceptibility findings. This study showed that the D-test should be used as an obligatory method in routine disk diffusion assay to detect inducible clindamycin resistance in MRSA so that effective antibiotic treatment can be provided.
-
Citations
Citations to this article as recorded by  - Characterization of oxacillin-resistant Staphylococcus lugdunensis isolated from sterile body fluids in a medical center in Taiwan: A 12-year longitudinal epidemiological study
Shih-Cheng Chang, Jazon Harl Hidrosollo, Lee-Chung Lin, Yu-Hsiang Ou, Cheng-Yen Kao, Jang-Jih Lu
Journal of Microbiology, Immunology and Infection.2022;[Epub] CrossRef - New update on molecular diversity of clinical Staphylococcus aureus isolates in Iran: antimicrobial resistance, adhesion and virulence factors, biofilm formation and SCCmec typing
Mahtab Tabandeh, Hami Kaboosi, Mojtaba Taghizadeh Armaki, Abazar Pournajaf, Fatemeh Peyravii Ghadikolaii
Molecular Biology Reports.2022; 49(4): 3099. CrossRef - Inducible Clindamycin-Resistant Staphylococcus aureus Strains in Africa: A Systematic Review
Muluneh Assefa, Faham Khamesipour
International Journal of Microbiology.2022; 2022: 1. CrossRef - Genetic Diversity and Virulence Profile of Methicillin and Inducible Clindamycin-Resistant Staphylococcus aureus Isolates in Western Algeria
Zahoua Mentfakh Laceb, Seydina M. Diene, Rym Lalaoui, Mabrouk Kihal, Fella Hamaidi Chergui, Jean-Marc Rolain, Linda Hadjadj
Antibiotics.2022; 11(7): 971. CrossRef - Characteristics of antibiotic resistance gene distribution in rainfall runoff and combined sewer overflow
Xin-rong Pan, Lei Chen, Li-ping Zhang, Jian-e Zuo
Environmental Science and Pollution Research.2022;[Epub] CrossRef - Staphylococcus aureus from Subclinical Cases of Mastitis in Dairy Cattle in Poland, What Are They Hiding? Antibiotic Resistance and Virulence Profile
Edyta Kaczorek-Łukowska, Joanna Małaczewska, Patrycja Sowińska, Marta Szymańska, Ewelina Agnieszka Wójcik, Andrzej Krzysztof Siwicki
Pathogens.2022; 11(12): 1404. CrossRef - Surveillance of osteoarticular infections caused by Staphylococcus aureus in a paediatric hospital in Mexico City
Nancy Evelyn Aguilar-Gómez, Jocelin Merida-Vieyra, Oscar Daniel Isunza-Alonso, María Gabriela Morales-Pirela, Oscar Colín-Martínez, Enrique Josué Juárez-Benítez, Silvestre García de la Puente, Alejandra Aquino-Andrade
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Molecular typing, biofilm formation, and analysis of adhesion factors in Staphylococcus aureus strains isolated from urinary tract infections
Masoumeh Navidinia, Anis Mohammadi, Reza Arjmand, Masoud Dadashi, Mehdi Goudarzi
Gene Reports.2021; 22: 101008. CrossRef - Molecular characterization of invasive Staphylococcus aureus strains isolated from patients with diabetes in Iran: USA300 emerges as the major type
Zahra Tayebi, Maryam Fazeli, Ali Hashemi, Saeed Abdi, Masoud Dadashi, Mohammad Javad Nasiri, Mehdi Goudarzi
Infection, Genetics and Evolution.2021; 87: 104679. CrossRef - Predominance of PVL-negative community-associated methicillin-resistant Staphylococcus aureus sequence type 8 in newly diagnosed HIV-infected adults, Tanzania
Joel Manyahi, Sabrina J. Moyo, Said Aboud, Nina Langeland, Bjørn Blomberg
European Journal of Clinical Microbiology & Infect.2021; 40(7): 1477. CrossRef - Molecular characterization of Staphylococcus aureus strains isolated from hospitalized patients based on coagulase gene polymorphism analysis: High frequency of vancomycin-intermediate S. aureus and the emergence of coagulase type II in Iran
Malihe Soltani, Bahareh Hajikhani, Samira Zamani, Mehrdad Haghighi, Ali Hashemi, Mohammad Javad Nasiri, Masoud Dadashi, Behzad Pourhossein, Mehdi Goudarzi
Gene Reports.2021; 23: 101078. CrossRef - Inducible clindamycin resistance among clinical Staphylococcus aureus strains in Iran: A contemporaneous systematic review and meta-analysis
Mojtaba Memariani, Hamed Memariani, Hamideh Moravvej
Gene Reports.2021; 23: 101104. CrossRef - Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients
Amr S. Bishr, Salma M. Abdelaziz, Ibrahim S. Yahia, Mahmoud A. Yassien, Nadia A. Hassouna, Khaled M. Aboshanab
Biology.2021; 10(7): 624. CrossRef - Relationship between MLSB resistance and the prevalent virulence genotypes among Bulgarian Staphylococcus aureus isolates
Virna-Maria Tsitou, Ivan Mitov, Raina Gergova
Acta Microbiologica et Immunologica Hungarica.2021; 68(1): 55. CrossRef - Occurrence, Antibiotic Resistance, Virulence Factors, and Genetic Diversity of Bacillus spp. from Public Hospital Environments in South Africa
Zamile N. Mbhele, Christiana O. Shobo, Daniel G. Amoako, Oliver T. Zishiri, Linda A. Bester
Microbial Drug Resistance.2021; 27(12): 1692. CrossRef - Bacterial Antibiotic Resistance: The Most Critical Pathogens
Giuseppe Mancuso, Angelina Midiri, Elisabetta Gerace, Carmelo Biondo
Pathogens.2021; 10(10): 1310. CrossRef - Mechanisms of Resistance to Macrolide Antibiotics among Staphylococcus aureus
Maria Miklasińska-Majdanik
Antibiotics.2021; 10(11): 1406. CrossRef - Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Human Bloodstream Infections: Detection of MLSB Resistance
Vanessa Silva, Sara Hermenegildo, Catarina Ferreira, Célia M. Manaia, Rosa Capita, Carlos Alonso-Calleja, Isabel Carvalho, José Eduardo Pereira, Luis Maltez, José L. Capelo, Gilberto Igrejas, Patrícia Poeta
Antibiotics.2020; 9(7): 375. CrossRef -
Genetic analysis of methicillin‐susceptible
Staphylococcus aureus
clinical isolates: High prevalence of multidrug‐resistant ST239 with strong biofilm‐production ability
Hossein Goudarzi, Mehdi Goudarzi, Fattaneh Sabzehali, Maryam Fazeli, Alireza Salimi Chirani
Journal of Clinical Laboratory Analysis.2020;[Epub] CrossRef - Emergence and spread of coagulase type III and staphylococcal cassette chromosome mec type IV among mupirocin-resistant Staphylococcus aureus isolated from wound infections
Mirmohammad Miri, Maryam Fazeli, Anahita Amirpour, Mohammad Javad Nasiri, Ramin Pouriran, Mehdi Goudarzi
Gene Reports.2020; 21: 100858. CrossRef - Resistance profile to antimicrobials agents in methicillin-resistant Staphylococcus aureus isolated from hospitals in South Brazil between 2014-2019
Adriana Medianeira Rossato, Muriel Primon-Barros, Lisiane da Luz Rocha, Keli Cristine Reiter, Cícero Armídio Gomes Dias, Pedro Alves d’Azevedo
Revista da Sociedade Brasileira de Medicina Tropic.2020;[Epub] CrossRef
|
