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Mohammad Mehdi Soltan Dallal 2 Articles
Profiling of Virulence-associated Factors in Shigella Species Isolated from Acute Pediatric Diarrheal Samples in Tehran, Iran
Sajad Yaghoubi, Reza Ranjbar, Mohammad Mehdi Soltan Dallal, Somayeh Yasliani Fard, Mohammad Hasan Shirazi, Mahmood Mahmoudi
Osong Public Health Res Perspect. 2017;8(3):220-226.   Published online June 30, 2017
DOI: https://doi.org/10.24171/j.phrp.2017.8.3.09
  • 2,977 View
  • 56 Download
  • 14 Citations
AbstractAbstract PDF
Objectives

The genus Shigella comprises the most infectious and diarrheagenic bacteria causing severe diseases, mostly in children under five years of age. This study aimed to detect nine virulence genes (ipaBCD, VirA, sen, set1A, set1B, ial, ipaH, stx, and sat) in Shigella species (spp.) using multiplex polymerase chain reaction (MPCR) and to determine the relation of Shigella spp. from pediatric diarrheal samples with hospitalization and bloody diarrhea in Tehran, Iran.

Methods

Shigella spp. were isolated and identified using standard microbiological and serological methods. The virulence genes were detected using MPCR.

Results

Seventy-five Shigella spp. (40 S. sonnei, 33 S. flexneri, 1 S. dysenteriae, and 1 S. boydii) were isolated in this study. The prevalence of ial, sen, sat, set1A, and set1B was 74.7%, 45.4%, 28%, 24%, and 24%, respectively. All S. flexneri isolates, while no S. sonnei, S. dysenteriae, or S. boydii isolates, contained sat, set1A, and set1B. All isolates were positive for ipaH, ipaBCD, and virA, while one (1.4%) of the isolates contained stx. The highest prevalence of virulence determinants was found in S. flexneri serotype IIa. Nineteen (57.6%) of 33 S. flexneri isolates were positive for ipaBCD, ipaH, virA, ial, and sat. The sen determinants were found to be statistically significantly associated with hospitalization and bloody diarrhea (p = 0.001).

Conclusion

This study revealed a high prevalence of enterotoxin genes in S. flexneri, especially in serotype 2a, and has presented relations between a few clinical features of shigellosis and numerous virulence determinants of clinical isolates of Shigella spp.

Citations

Citations to this article as recorded by  
  • Distribution of genes encoding virulence factors of Shigella strains isolated from children with diarrhea in southwest Iran
    Nabi Jomehzadeh, Khadijah Ahmadi, Hazhir Javaherizadeh, Maryam Afzali
    Molecular Biology Reports.2021; 48(2): 1645.     CrossRef
  • Evaluate the distribution of virulence genes and to investigate antibiotic resistance pattern among Shigella species isolated from children with shigellosis in Iran
    Samane Mohebi, Hossein Hosseini Nave, Kasra Javadi, Ali Amanati, Soudeh Kholdi, Mahtab Hadadi, Zahra Hashemizadeh, Mohammad Motamedifar
    Gene Reports.2021; 23: 101189.     CrossRef
  • Burden, Antibiotic Resistance, and Clonality of Shigella spp. Implicated in Community-Acquired Acute Diarrhoea in Lilongwe, Malawi
    Abel F.N.D. Phiri, Akebe Luther King Abia, Daniel Gyamfi Amoako, Rajab Mkakosya, Arnfinn Sundsfjord, Sabiha Y. Essack, Gunnar Skov Simonsen
    Tropical Medicine and Infectious Disease.2021; 6(2): 63.     CrossRef
  • Frequency and Antimicrobial Resistance of Shigella Species in Iran During 2000-2020
    Farhad Moradi, Nahal Hadi, Maryam Akbari, Zahra Hashemizadeh, Reyhaneh Rouhi Jahromi
    Jundishapur Journal of Health Sciences.2021;[Epub]     CrossRef
  • Virulence factors and molecular characteristics of Shigella flexneri isolated from calves with diarrhea
    Zhen Zhu, Weiwei Wang, Mingze Cao, Qiqi Zhu, Tenghe Ma, Yongying Zhang, Guanhui Liu, Xuzheng Zhou, Bing Li, Yuxiang Shi, Jiyu Zhang
    BMC Microbiology.2021;[Epub]     CrossRef
  • Development of quinolone resistance and prevalence of different virulence genes among Shigella flexneri and Shigella dysenteriae in environmental water samples
    B. Roy, S.K. Tousif Ahamed, B. Bandyopadhyay, N. Giri
    Letters in Applied Microbiology.2020; 71(1): 86.     CrossRef
  • High Rates of Antimicrobial Resistance and Virulence Gene Distribution Among Shigella spp. Isolated from Pediatric Patients in Tehran, Iran


    Mohammadmahdi Karimi-Yazdi, Zohreh Ghalavand, Mahdi Shabani, Hamidreza Houri, Mehrzad Sadredinamin, Marzieh Taheri, Gita Eslami
    Infection and Drug Resistance.2020; Volume 13: 485.     CrossRef
  • Molecular characterization of Shigella species isolated from diarrheal patients in Tehran, Iran: phylogenetic typing and its association with virulence gene profiles and a novel description of Shigella invasion associated locus
    Sina Arabshahi, Aytak Novinrooz, Reza Ranjbar, Abbas Ali Imani Fooladi
    European Journal of Clinical Microbiology & Infect.2020; 39(9): 1727.     CrossRef
  • Case report on a swift shift in uropathogens from Shigella flexneri to Escherichia coli: a thin line between bacterial persistence and reinfection
    Kukwah Anthony Tufon, Djike Puepi Yolande Fokam, Youmbi Sylvain Kouanou, Henry Dilonga Meriki
    Annals of Clinical Microbiology and Antimicrobials.2020;[Epub]     CrossRef
  • Virulence-related genes are associated with clinical and nutritional outcomes of Shigella/Enteroinvasive Escherichia coli pathotype infection in children from Brazilian semiarid region: A community case-control study
    Mariana Bona, Pedro Henrique Medeiros, Ana Karolina Santos, Thiago Freitas, Mara Prata, Herlice Veras, Marília Amaral, Daniel Oliveira, Alexandre Havt, Aldo Ângelo Lima
    International Journal of Medical Microbiology.2019; 309(2): 151.     CrossRef
  • Genotyping and diversity of virulence genes among Shigella sonnei isolated from children with diarrhoea
    Hamed Memariani, Mojtaba Memariani
    Reviews in Medical Microbiology.2019; 30(4): 217.     CrossRef
  • Virulence gene profiles of Shigella species isolated from stool specimens in India: its association with clinical manifestation and antimicrobial resistance
    Dhiviya Prabaa Muthuirulandi Sethuvel, Shalini Anandan, Joy Sarojini Michael, Dhivya Murugan, Ayyanraj Neeravi, Valsan Philip Verghese, Kamini Walia, Balaji Veeraraghavan
    Pathogens and Global Health.2019; 113(4): 173.     CrossRef
  • Prevalence of enterotoxin-encoding genes among diverse Shigella strains isolated from patients with diarrhea, southwest Iran
    Mojtaba Moosavian, Sakineh Seyed-Mohammadi, Ahmad Farajzadeh Sheikh, Saeed Khoshnood, Aram Asarehzadegan Dezfuli, Morteza Saki, Gholamreza Ghaderian, Fatemeh Shahi, Mahtab Abdi, Fariba Abbasi
    Acta Microbiologica et Immunologica Hungarica.2018; 66(1): 91.     CrossRef
  • Frequency of Mutations in Quinolone Resistance-Determining Regions and Plasmid-Mediated Quinolone Resistance in Shigella Isolates Recovered from Pediatric Patients in Tehran, Iran: An Overlooked Problem
    Sajad Yaghoubi, Reza Ranjbar, Mohammad Mehdi Soltan Dallal, Mohammad Hasan Shirazi, Mohammad Kazem Sharifi-Yazdi
    Microbial Drug Resistance.2018; 24(6): 699.     CrossRef
The Effect of Lactobacillus acidophilus PTCC 1643 on Cultured Intestinal Epithelial Cells Infected with Salmonella enterica serovar Enteritidis
Mona Moshiri, Mohammad Mehdi Soltan Dallal, Farhad Rezaei, Masoumeh Douraghi, Laleh Sharifi, Zahra Noroozbabaei, Mehrdad Gholami, Abbas Mirshafiey
Osong Public Health Res Perspect. 2017;8(1):54-60.   Published online February 28, 2017
DOI: https://doi.org/10.24171/j.phrp.2017.8.1.07
  • 2,731 View
  • 20 Download
  • 8 Citations
AbstractAbstract PDF
Objectives

Gastrointestinal disorders caused by Salmonella enterica serovar Enteritidis (SesE) are a significant health problem around the globe. Probiotic bacteria have been shown to have positive effects on the immune responses. Lactobacillus acidophilus was examined for its capability to influence the innate immune response of HT29 intestinal epithelial cells towards SesE. The purpose of this work was to assess the effect of L. acidophilus PTCC 1643 on cultured intestinal epithelial cells infected with SesE.

Methods

HT29 cells were cultured in Roswell Park Memorial Institute medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. The cells were treated with L. acidophilus PTCC 1643 after or before challenge with SesE. At 2 and 4 hours post-infection, we measured changes in the expression levels of TLR2 and TLR4 via real-time polymerase chain reaction.

Results

Treatment with L. acidophilus inhibited SesE-induced increases in TLR2 and TLR4 expression in the infected HT29 cells. Moreover, the expression of TLR2 and TLR4 in cells that were pretreated with L. acidophilus and then infected with SesE was significantly higher than that in cells infected with SesE without pretreatment. Taken together, the results indicated that L. acidophilus had an anti-inflammatory effect and modulated the innate immune response to SesE by influencing TLR2 and TLR4 expression.

Conclusion

Our findings suggested that L. acidophilus PTCC 1643 was able to suppress inflammation caused by SesE infection in HT29 cells and reduce TLR2 and TLR4 expression. Additional in vivo and in vitro studies are required to further elucidate the mechanisms underlying this anti-inflammatory effect.

Citations

Citations to this article as recorded by  
  • Lactobacillus acidophilus ATCC 4356 Exopolysaccharides Suppresses Mediators of Inflammation through the Inhibition of TLR2/STAT-3/P38-MAPK Pathway in DEN-Induced Hepatocarcinogenesis in Rats
    Ola M. S. Khedr, Sawsan M. El-Sonbaty, Fatma S. M. Moawed, Eman I. Kandil, Basma E. Abdel-Maksoud
    Nutrition and Cancer.2022; 74(3): 1037.     CrossRef
  • Osmoporation is a versatile technique to encapsulate fisetin using the probiotic bacteria Lactobacillus acidophilus
    Eduardo Wagner Vasconcelos de Andrade, Sebastien Dupont, Laurent Beney, Roberta Targino Hoskin, Márcia Regina da Silva Pedrini
    Applied Microbiology and Biotechnology.2022; 106(3): 1031.     CrossRef
  • The Game for Three: Salmonella–Host–Microbiota Interaction Models
    Krzysztof Grzymajlo
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • The Functional Roles of Lactobacillus acidophilus in Different Physiological and Pathological Processes
    Huijuan Gao, Xin Li, Xiatian Chen, Deng Hai, Chuang Wei, Lei Zhang, Peifeng Li
    Journal of Microbiology and Biotechnology.2022; 32(10): 1226.     CrossRef
  • Improving bioactive properties of peach juice using Lactobacillus strains fermentation: Antagonistic and anti-adhesion effects, anti-inflammatory and antioxidant properties, and Maillard reaction inhibition
    Seyed Mohammad Bagher Hashemi, Dornoush Jafarpour, Mohammad Jouki
    Food Chemistry.2021; 365: 130501.     CrossRef
  • The immune regulatory role of Lactobacillus acidophilus: An updated meta-analysis of randomized controlled trials
    Wei Zhao, Yangshuo Liu, Lai-Yu Kwok, Tiequan Cai, Wenyi Zhang
    Food Bioscience.2020; 36: 100656.     CrossRef
  • Gene expression changes as predictors of the immune-modulatory effects of probiotics: Towards a better understanding of strain-disease specific interactions
    Frida Gorreja
    NFS Journal.2019; 14-15: 1.     CrossRef
  • A review on anti-adhesion therapies of bacterial diseases
    Arezoo Asadi, Shabnam Razavi, Malihe Talebi, Mehrdad Gholami
    Infection.2019; 47(1): 13.     CrossRef
Mohammad Mehdi Soltan Dallal 1 Article
Identification of Klebsiella pneumoniae Carbapenemase-producing Klebsiella oxytoca in Clinical Isolates in Tehran Hospitals, Iran by Chromogenic Medium and Molecular Methods
Majid Validi, Mohammad Mehdi Soltan Dallal, Masoumeh Douraghi, Jalil Fallah Mehrabadi, Abbas Rahimi Foroushani
Osong Public Health Res Perspect. 2016;7(5):301-306.   Published online October 31, 2016
DOI: https://doi.org/10.1016/j.phrp.2016.08.006
  • 1,796 View
  • 25 Download
  • 8 Citations
AbstractAbstract PDF
Objectives
Production of carbapenemase, especially Klebsiella pneumoniae carbapenemases (KPC), is one of the antibiotic resistance mechanisms of Enterobacteriaceae such as Klebsiella oxytoca. This study aimed to investigate and identify KPC-producing K. oxytoca isolates using molecular and phenotypic methods.
Methods
A total of 75 isolates of K. oxytoca were isolated from various clinical samples, and were verified as K. oxytoca after performing standard microbiological tests and using a polymerase chain reaction (PCR) method. An antibiotic susceptibility test was performed using a disc diffusion method according to the Clinical and Laboratory Standards Institute guidelines. CHROMagar KPC chromogenic culture media was used to examine and confirm the production of the carbapenemase enzyme in K. oxytoca isolates; in addition, PCR was used to evaluate the presence of blaKPC gene in K. oxytoca strains.
Results
Of a total of 75 K. oxytoca isolates, one multidrug resistant strain was isolated from the urine of a hospitalized woman. This strain was examined to assess its ability to produce carbapenemase enzyme; it produced a colony with a blue metallic color on the CHROMagar KPC chromogenic culture media. In addition, the blaKPC gene was confirmed by PCR. After sequencing, it was confirmed and deposited in GenBank.
Conclusion
To date, many cases of KPC-producing Enterobacteriaceae, in particular K. pneumoniae, have been reported in different countries; there are also some reports on the identification of KPC-producing K. oxytoca. Therefore, to prevent the outbreak of nosocomial infections, the early detection, control, and prevention of the spread of these strains are of great importance.

Citations

Citations to this article as recorded by  
  • Klebsiella oxytoca Complex: Update on Taxonomy, Antimicrobial Resistance, and Virulence
    Jing Yang, Haiyan Long, Ya Hu, Yu Feng, Alan McNally, Zhiyong Zong
    Clinical Microbiology Reviews.2022;[Epub]     CrossRef
  • Development and comparison of immunochromatographic strips with four nanomaterial labels: Colloidal gold, new colloidal gold, multi-branched gold nanoflowers and Luminol-reduced Au nanoparticles for visual detection of Vibrio parahaemolyticus in seafood
    Meijiao Wu, Youxue Wu, Cheng Liu, Yachen Tian, Shuiqin Fang, Hao Yang, Bin Li, Qing Liu
    Aquaculture.2021; 539: 736563.     CrossRef
  • Variation in Accessory Genes Within the Klebsiella oxytoca Species Complex Delineates Monophyletic Members and Simplifies Coherent Genotyping
    Amar Cosic, Eva Leitner, Christian Petternel, Herbert Galler, Franz F. Reinthaler, Kathrin A. Herzog-Obereder, Elisabeth Tatscher, Sandra Raffl, Gebhard Feierl, Christoph Högenauer, Ellen L. Zechner, Sabine Kienesberger
    Frontiers in Microbiology.2021;[Epub]     CrossRef
  • Multidrug-Resistant Bacteria and Alternative Methods to Control Them: An Overview
    Roberto Vivas, Ana Andréa Teixeira Barbosa, Silvio Santana Dolabela, Sona Jain
    Microbial Drug Resistance.2019; 25(6): 890.     CrossRef
  • Molecular typing of cytotoxin-producing Klebsiella oxytoca isolates by 16S-23S internal transcribed spacer PCR
    M.M. Soltan Dallal, M. Validi, M. Douraghi, B. Bakhshi
    New Microbes and New Infections.2019; 30: 100545.     CrossRef
  • Determination of antibiotic resistance and minimum inhibitory concentration of meropenem and imipenem growth in Klebsiella strains isolated from urinary tract infection in Shahrekord educational hospitals
    Farshad Kakian, Behnam Zamzad, Abolfazl Gholipour, Kiarash Zamanzad
    Journal of Shahrekord University of Medical Scienc.2019; 21(2): 80.     CrossRef
  • Evaluation the cytotoxic effect of cytotoxin-producing Klebsiella oxytoca isolates on the HEp-2 cell line by MTT assay
    Mohammad Mehdi Soltan-Dallal, Majid Validi, Masoumeh Douraghi, Jalil Fallah-Mehrabadi, Leila Lormohammadi
    Microbial Pathogenesis.2017; 113: 416.     CrossRef
  • Outbreak by Hypermucoviscous Klebsiella pneumoniae ST11 Isolates with Carbapenem Resistance in a Tertiary Hospital in China
    Lingling Zhan, Shanshan Wang, Yinjuan Guo, Ye Jin, Jingjing Duan, Zhihao Hao, Jingnan Lv, Xiuqin Qi, Longhua Hu, Liang Chen, Barry N. Kreiswirth, Rong Zhang, Jingye Pan, Liangxing Wang, Fangyou Yu
    Frontiers in Cellular and Infection Microbiology.2017;[Epub]     CrossRef

PHRP : Osong Public Health and Research Perspectives