Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives

OPEN ACCESS
SEARCH
Search

Author index

Page Path
HOME > Articles and issues > Author index
Search
Mohammad Chehelgerdi 1 Article
TEM and SHV Genes in Klebsiella pneumoniae Isolated from Cockroaches and Their Antimicrobial Resistance Pattern
Abbas Doosti, Mohammad Pourabbas, Asghar Arshi, Mohammad Chehelgerdi, Hamidreza Kabiri
Osong Public Health Res Perspect. 2015;6(1):3-8.   Published online February 28, 2015
DOI: https://doi.org/10.1016/j.phrp.2014.10.011
  • 3,747 View
  • 51 Download
  • 16 Crossref
AbstractAbstract PDF
Objectives
Klebsiella pneumoniae is a gram-negative rod bacterium, a known cause of community-acquired bacterial pneumonia and is an important hospital-acquired pathogen that causes severe morbidity and mortality. The aim of this study was to identify the TEM and SHV genes in K. pneumoniae isolated from cockroaches obtained from hospitals.
Methods
In this study, 250 cockroaches were collected from different hospitals in the province of Chaharmahal Va Bakhtiari, which is located in southwest Iran. The samples were examined for the presence of K. pneumoniae by plating onto a combination of culture media, and the antimicrobial susceptibility patterns of isolated K. pneumoniae from samples were evaluated using the disk diffusion test. In addition, from the culture, genomic bacterial DNA was extracted, and sequence-specific targets (TEM and SHV genes) were amplified using the polymerase chain reaction (PCR) method.
Results
Out of 250 cockroach samples collected from various hospitals, 179 samples (71.60%) were positive for K. pneumoniae. PCR reaction was performed using specific oligonucleotide primers (TEM-F, TEM-R and SHV-F, SHV-R) for the amplification of each gene, and amplified products were visualized on 1% agarose gel electrophoresis. Of all the specimens amplified by PCR in this research, 32 samples (17.87%) were positive for TEM and 15 samples (8.37%) were positive for SHV.
Conclusion
Detection of TEM and SHV genes using molecular methods and their pattern of antimicrobial resistance can provide useful information about the epidemiology of and risk factors associated with K. pneumoniae infection.

Citations

Citations to this article as recorded by  
  • Review: Antimicrobial resistance of Klebsiella pneumoniae isolated from poultry, cattle and pigs
    B. Kot, M. Witeska
    animal.2024; 18(11): 101345.     CrossRef
  • Is It Time to Start Worrying? A Comprehensive Report on the Three-Year Prevalence of ESBL-Producing Bacteria and Their Trends in Antibiotic Resistance from the Largest University Hospital in Slovakia
    Yashar Jalali, Andrea Kološová, Adriána Liptáková, Ján Kyselovič, Anna Oleárová, Monika Jalali, Juraj Payer
    Pharmaceuticals.2024; 17(11): 1517.     CrossRef
  • Novel adjuvant nano-vaccine induced immune response against Acinetobacter baumannii
    Tohid Piri-Gharaghie, Abbas Doosti, Seyed Abbas Mirzaei
    AMB Express.2023;[Epub]     CrossRef
  • Virulence factors, antibiotic resistance patterns, and molecular types of clinical isolates of Klebsiella Pneumoniae
    Mitra Ahmadi, Reza Ranjbar, Payam Behzadi, Taher Mohammadian
    Expert Review of Anti-infective Therapy.2022; 20(3): 463.     CrossRef
  • Detection of Klebsiella pneumoniae antibiotic-resistant genes: An impending source of multidrug resistance dissemination through raw food
    Kashaf Junaid, Hasan Ejaz, Sonia Younas, Awadh Alanazi, Humaira Yasmeen, Abdul Rehman
    Saudi Journal of Biological Sciences.2022; 29(5): 3347.     CrossRef
  • First Report of Extended-Spectrum β-Lactamase ( bla CTX-M1 ) and Colistin Resistance Gene mcr-1 in E. coli of Lineage ST648 from Cockroaches in Tunisia
    Sarrah Landolsi, Rachid Selmi, Linda Hadjadj, Asma Ben Haj Yahia, Kaouther Ben Romdhane, Lilia Messadi, Jean Marc Rolain, Courtney J. Robinson
    Microbiology Spectrum.2022;[Epub]     CrossRef
  • Phenotypic and genotypic detection of extended spectrum beta lactamase enzyme in Klebsiella pneumoniae
    Aso Bakr Mohammed, Khanda Abdullateef Anwar, Monica Cartelle Gestal
    PLOS ONE.2022; 17(9): e0267221.     CrossRef
  • Virulence factors and antibiotic resistance properties of Streptococcus species isolated from hospital cockroaches
    Mohammad Chehelgerdi, Reza Ranjbar
    3 Biotech.2021;[Epub]     CrossRef
  • Putative novel B-cell vaccine candidates identified by reverse vaccinology and genomics approaches to control Acinetobacter baumannii serotypes
    Sheida Beiranvand, Abbas Doosti, Seyed Abbas Mirzaei
    Infection, Genetics and Evolution.2021; 96: 105138.     CrossRef
  • Molecular epidemiology of Carbapenem-resistant Klebsiella pneumoniae in a paediatric hospital in China
    Bingjie Wang, Fen Pan, Chun Wang, Wantong Zhao, Yan Sun, Tiandong Zhang, Yingying Shi, Hong Zhang
    International Journal of Infectious Diseases.2020; 93: 311.     CrossRef
  • Fecal Carriage and Molecular Epidemiology of Carbapenem-Resistant Enterobacteriaceae from Inpatient Children in a Pediatric Hospital of Shanghai


    Qi Xu, Fen Pan, Yan Sun, Chun Wang, Yingying Shi, Tiandong Zhang, Fangyuan Yu, Hong Zhang
    Infection and Drug Resistance.2020; Volume 13: 4405.     CrossRef
  • Contamination of Cockroaches (Insecta: Blattaria) by Medically Important Bacteriae: A Systematic Review and Meta-analysis
    Hassan Nasirian, Michael Rust
    Journal of Medical Entomology.2019; 56(6): 1534.     CrossRef
  • Competition assays between ESBL-producing E. coli and K. pneumoniae isolates collected from Lebanese elderly: An additional cost on fitness
    Nourhane Hafza, Caren Challita, Iman Dandachi, Mounir Bousaab, Elias Dahdouh, Ziad Daoud
    Journal of Infection and Public Health.2018; 11(3): 393.     CrossRef
  • Synthesis, characterization, antiproliferative, and antimicrobial activity of osmium(II) half-sandwich complexes
    Joel M. Gichumbi, Holger B. Friedrich, Bernard Omondi, Kovashnee Naicker, Moganavelli Singh, Hafizah Y. Chenia
    Journal of Coordination Chemistry.2018; 71(2): 342.     CrossRef
  • Relationship between β-lactamase production and resistance phenotype in Klebsiella pneumoniae strains
    Jeongjin Kim, Tian Ding, Juhee Ahn
    FEMS Microbiology Letters.2017;[Epub]     CrossRef
  • First Report of German Cockroaches (Blattella germanica) as Reservoirs of CTX-M-15 Extended-Spectrum-β-Lactamase- and OXA-48 Carbapenemase-Producing Enterobacteriaceae in Batna University Hospital, Algeria
    Lotfi Loucif, Djamila Gacemi-Kirane, Zineb Cherak, Naima Chamlal, Nadia Grainat, Jean-Marc Rolain
    Antimicrobial Agents and Chemotherapy.2016; 60(10): 6377.     CrossRef

PHRP : Osong Public Health and Research Perspectives
TOP