- Detection and Isolation of SARS-CoV-2 in Serum, Urine, and Stool Specimens of COVID-19 Patients from the Republic of Korea
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Jeong-Min Kim, Heui Man Kim, Eun Jung Lee, Hye Jun Jo, Youngsil Yoon, Nam-Joo Lee, Junseock Son, Ye-Ji Lee, Mi Seon Kim, Yong-Pyo Lee, Su-Jin Chae, Kye Ryeong Park, Seung-Rye Cho, Sehee Park, Su Jin Kim, Eunbyeol Wang, SangHee Woo, Aram Lim, Su-Jin Park, JunHyeong Jang, Yoon-Seok Chung, Bum Sik Chin, Jin-Soo Lee, Duko Lim, Myung-Guk Han, Cheon Kwon Yoo
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Osong Public Health Res Perspect. 2020;11(3):112-117. Published online May 8, 2020
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DOI: https://doi.org/10.24171/j.phrp.2020.11.3.02
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- Objectives
Coronavirus Disease-19 (COVID-19) is a respiratory infection characterized by the main symptoms of pneumonia and fever. It is caused by the novel coronavirus severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2), which is known to spread via respiratory droplets. We aimed to determine the rate and likelihood of SARS-CoV-2 transmission from COVID-19 patients through non-respiratory routes.
Methods
Serum, urine, and stool samples were collected from 74 hospitalized patients diagnosed with COVID-19 based on the detection of SARS-CoV-2 in respiratory samples. The SARS-CoV-2 RNA genome was extracted from each specimen and real-time reverse transcription polymerase chain reaction performed. CaCo-2 cells were inoculated with the specimens containing the SARS-COV-2 genome, and subcultured for virus isolation. After culturing, viral replication in the cell supernatant was assessed.
Results
Of the samples collected from 74 COVID-19 patients, SARS-CoV-2 was detected in 15 serum, urine, or stool samples. The virus detection rate in the serum, urine, and stool samples were 2.8% (9/323), 0.8% (2/247), and 10.1% (13/129), and the mean viral load was 1,210 ± 1,861, 79 ± 30, and 3,176 ± 7,208 copy/µL, respectively. However, the SARS-CoV-2 was not isolated by the culture method from the samples that tested positive for the SARS-CoV-2 gene.
Conclusion
While the virus remained detectable in the respiratory samples of COVID-19 patients for several days after hospitalization, its detection in the serum, urine, and stool samples was intermittent. Since the virus could not be isolated from the SARS-COV-2-positive samples, the risk of viral transmission via stool and urine is expected to be low.
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Citations
Citations to this article as recorded by  - Norovirus and Coronavirus Risks in Food Service Settings: A Systematic Review for Future Research
Bendegul Okumus
Journal of Culinary Science & Technology.2023; 21(1): 71. CrossRef - Occurrence of SARS-CoV-2 in excreta, sewage, and environment: epidemiological significance and potential risks
Omar Amahmid, Youssef El Guamri, Youness Rakibi, Saadia Ouizat, Mohamed Yazidi, Bouchra Razoki, Khadija Kaid Rassou, Souad Asmama, Khadija Bouhoum, Driss Belghyti
International Journal of Environmental Health Rese.2022; 32(8): 1686. CrossRef - Twelve Months with COVID-19: What Gastroenterologists Need to Know
Giulia Concas, Michele Barone, Ruggiero Francavilla, Fernanda Cristofori, Vanessa Nadia Dargenio, Rossella Giorgio, Costantino Dargenio, Vassilios Fanos, Maria Antonietta Marcialis
Digestive Diseases and Sciences.2022; 67(7): 2771. CrossRef - SARS-CoV-2 loads in urine, sera and stool specimens in association with clinical features of COVID-19 patients
Déborah Anjos, Fabiola Souza Fiaccadori, Carolina do Prado Servian, Simone Gonçalves da Fonseca, Adriana Oliveira Guilarde, Moara Alves Santa Bárbara Borges, Fernanda Craveiro Franco, Bergmann Morais Ribeiro, Menira Souza
Journal of Clinical Virology Plus.2022; 2(1): 100059. CrossRef - Absence of virological and epidemiological evidence that SARS-CoV-2 poses COVID-19 risks from environmental fecal waste, wastewater and water exposures
Mark D. Sobsey
Journal of Water and Health.2022; 20(1): 126. CrossRef - Discrimination and surveillance of infectious severe acute respiratory syndrome Coronavirus 2 in wastewater using cell culture and RT-qPCR
Silvia Monteiro, Daniela Rente, Mónica V. Cunha, Tiago A. Marques, Eugénia Cardoso, João Vilaça, Norberta Coelho, Nuno Brôco, Marta Carvalho, Ricardo Santos
Science of The Total Environment.2022; 815: 152914. CrossRef - A collaborative robotic solution to partly automate SARS-CoV-2 serological tests in small facilities
Andrea Maria Zanchettin, Federica Facciotti
SLAS Technology.2022; 27(1): 100. CrossRef - The Impact of COVID-19 on Blood Transfusion Services: A Systematic Review and Meta-Analysis
Cindy Chiem, Khader Alghamdi, Thao Nguyen, Joon Hee Han, Hongzhao Huo, Denise Jackson
Transfusion Medicine and Hemotherapy.2022; 49(2): 107. CrossRef - Development of an in vitro model for animal species susceptibility to SARS-CoV-2 replication based on expression of ACE2 and TMPRSS2 in avian cells
Darrell R. Kapczynski, Ryan Sweeney, Erica Spackman, Mary Pantin-Jackwood, David L. Suarez
Virology.2022; 569: 1. CrossRef - Kidney Injury in COVID-19: Epidemiology, Molecular Mechanisms and Potential Therapeutic Targets
J. Pedro Teixeira, Sharon Barone, Kamyar Zahedi, Manoocher Soleimani
International Journal of Molecular Sciences.2022; 23(4): 2242. CrossRef - SARS-CoV-2 shedding sources in wastewater and implications for wastewater-based epidemiology
Xuan Li, Jagadeeshkumar Kulandaivelu, Ying Guo, Shuxin Zhang, Jiahua Shi, Jake O’Brien, Sudipti Arora, Manish Kumar, Samendra P. Sherchan, Ryo Honda, Greg Jackson, Stephen P. Luby, Guangming Jiang
Journal of Hazardous Materials.2022; 432: 128667. CrossRef - An Ensemble Learning Model for COVID-19 Detection from Blood Test Samples
Olusola O. Abayomi-Alli, Robertas Damaševičius, Rytis Maskeliūnas, Sanjay Misra
Sensors.2022; 22(6): 2224. CrossRef - Correlation of SARS‑CoV‑2 to cancer: Carcinogenic or anticancer? (Review)
Ying-Shuang Li, Hua-Cheng Ren, Jian-Hua Cao
International Journal of Oncology.2022;[Epub] CrossRef - Prolonged fecal shedding of SARS‐CoV‐2 in a young immunocompetent COVID‐19 patient: A case report and literature overview
Fang Xiao, Peiqi Wan, Qiuling Wei, Guiyang Wei, Yanhong Yu
Journal of Medical Virology.2022; 94(7): 3133. CrossRef - Lead time of early warning by wastewater surveillance for COVID-19: Geographical variations and impacting factors
Manish Kumar, Guangming Jiang, Alok Kumar Thakur, Shreya Chatterjee, Tanushree Bhattacharya, Sanjeeb Mohapatra, Tushara Chaminda, Vinay Kumar Tyagi, Meththika Vithanage, Prosun Bhattacharya, Long D. Nghiem, Dibyendu Sarkar, Christian Sonne, Jürgen Mahlkne
Chemical Engineering Journal.2022; 441: 135936. CrossRef - Possible Cross-Reactivity of Feline and White-Tailed Deer Antibodies against the SARS-CoV-2 Receptor Binding Domain
Trevor J. Hancock, Peyton Hickman, Niloo Kazerooni, Melissa Kennedy, Stephen A. Kania, Michelle Dennis, Nicole Szafranski, Richard Gerhold, Chunlei Su, Tom Masi, Stephen Smith, Tim E. Sparer, Tom Gallagher
Journal of Virology.2022;[Epub] CrossRef - Artificial neural network-based estimation of COVID-19 case numbers and effective reproduction rate using wastewater-based epidemiology
Guangming Jiang, Jiangping Wu, Jennifer Weidhaas, Xuan Li, Yan Chen, Jochen Mueller, Jiaying Li, Manish Kumar, Xu Zhou, Sudipti Arora, Eiji Haramoto, Samendra Sherchan, Gorka Orive, Unax Lertxundi, Ryo Honda, Masaaki Kitajima, Greg Jackson
Water Research.2022; 218: 118451. CrossRef - Partial sequence conservation of SARS‐CoV‐2 NSP‐2, NSP‐12, and Spike in stool samples from Abadan, Iran
Milad Zandi, Saber Soltani, Alireza Tabibzadeh, Sepideh Nasimzadeh, Emad Behboudi, Armin Zakeri, Yousef Erfani, Shokrollah Salmanzadeh, Samaneh Abbasi
Biotechnology and Applied Biochemistry.2022;[Epub] CrossRef - SARS-CoV-2 Viral Load in the Pulmonary Compartment of Critically Ill COVID-19 Patients Correlates with Viral Serum Load and Fatal Outcomes
Mario Ynga-Durand, Henrike Maaß, Marko Milošević, Fran Krstanović, Marina Pribanić Matešić, Stipan Jonjić, Alen Protić, Ilija Brizić, Alan Šustić, Luka Čičin-Šain
Viruses.2022; 14(6): 1292. CrossRef - Application of human RNase P normalization for the realistic estimation of SARS-CoV-2 viral load in wastewater: A perspective from Qatar wastewater surveillance
Shimaa S. El-Malah, Jayaprakash Saththasivam, Khadeeja Abdul Jabbar, Arun K.K., Tricia A. Gomez, Ayeda A. Ahmed, Yasmin A. Mohamoud, Joel A. Malek, Laith J. Abu Raddad, Hussein A. Abu Halaweh, Roberto Bertollini, Jenny Lawler, Khaled A. Mahmoud
Environmental Technology & Innovation.2022; 27: 102775. CrossRef - Predictors of intensive care unit admission and mortality in SARS-CoV-2 infection: A cross sectional study at a tertiary care hospital
Ahmed Alkhamis, Yousef Alshamali, Wassim Chehadeh, Amar Jasem, Abdulaziz Alsayed Omar, Mohammad Alghounaim, Hussein Elsaaran, Sarah Al-Youha, Sulaiman Almazeedi, Moh A. Alkhamis, Salman Alsabah
Annals of Medicine and Surgery.2022; 80: 104097. CrossRef - Vertical outbreak of COVID-19 in high-rise buildings: The role of sewer stacks and prevention measures
Ying Guo, Xuan Li, Stephen Luby, Guangming Jiang
Current Opinion in Environmental Science & Health.2022; 29: 100379. CrossRef - Current trends in COVID-19 diagnosis and its new variants in physiological fluids: Surface antigens, antibodies, nucleic acids, and RNA sequencing
Menna Mostafa, Ahmed Barhoum, Ekin Sehit, Hossam Gewaid, Eslam Mostafa, Mohamed M. Omran, Mohga S. Abdalla, Fatehy M. Abdel-Haleem, Zeynep Altintas, Robert J. Forster
TrAC Trends in Analytical Chemistry.2022; 157: 116750. CrossRef - The Persistence Time of SARS-CoV-2 RNA in Hospitalized COVID-19
Patients: A Prospective Study
Jila Yavarian, Mohammad Javaherian, Somayeh Shatizadeh Malekshahi, Zahra Ahmadinejad, Fatemeh Ghiasvand, Elaheh Ahmadi, Ladan Abbasian, Malihe Hasannezhad, Narges Dabaghipour, Nazanin-Zahra Shafiei-Jandaghi, Talat Mokhtari-Azad, Mahboubeh Hajiabdolbaghi
Infectious Disorders - Drug Targets.2022;[Epub] CrossRef - Evaluation of the presence of SARS-CoV-2 in vaginal and anal swabs of women with omicron variants of SARS-CoV-2 infection
Ding Liu, Yunfu Zhang, Dongfeng Chen, Xianhua Wang, Fuling Huang, Ling Long, Xiuhui Zheng
Frontiers in Microbiology.2022;[Epub] CrossRef - Immune-profiling of SARS-CoV-2 viremic patients reveals dysregulated innate immune responses
Xiaoming Sun, Ce Gao, Ke Zhao, Yanhui Yang, Yelizaveta Rassadkina, Jesse Fajnzylber, James Regan, Jonathan Z. Li, Mathias Lichterfeld, Xu G. Yu
Frontiers in Immunology.2022;[Epub] CrossRef - Pathogens inactivation in nutrient recovery from urine: A review
Kangning Xu, Jun Lu, Lanlan Hu, Jiyun Li, Shikun Cheng, Min Zheng, Chengwen Wang
Frontiers in Environmental Science.2022;[Epub] CrossRef - Severe acute respiratory syndrome coronavirus‐2: implications for blood safety and sufficiency
Philip Kiely, Veronica C. Hoad, Clive R. Seed, Iain B. Gosbell
Vox Sanguinis.2021; 116(2): 155. CrossRef - Is recurrence possible in coronavirus disease 2019 (COVID-19)? Case series and systematic review of literature
Anna Gidari, Marco Nofri, Luca Saccarelli, Sabrina Bastianelli, Samuele Sabbatini, Silvia Bozza, Barbara Camilloni, Igino Fusco-Moffa, Claudia Monari, Edoardo De Robertis, Antonella Mencacci, Daniela Francisci
European Journal of Clinical Microbiology & Infect.2021; 40(1): 1. CrossRef - Hunting coronavirus by transmission electron microscopy – a guide to SARS‐CoV‐2‐associated ultrastructural pathology in COVID‐19 tissues
Helmut Hopfer, Martin C Herzig, Rainer Gosert, Thomas Menter, Jürgen Hench, Alexandar Tzankov, Hans H Hirsch, Sara E Miller
Histopathology.2021; 78(3): 358. CrossRef - CRISPR-based biosensing systems: a way to rapidly diagnose COVID-19
Majid Vatankhah, Amir Azizi, Anahita Sanajouyan Langeroudi, Sajad Ataei Azimi, Imaneh Khorsand, Mohammad Amin Kerachian, Jamshid Motaei
Critical Reviews in Clinical Laboratory Sciences.2021; 58(4): 225. CrossRef - The virus that shook the world: questions and answers about SARS-CoV-2 and COVID-19
Radostina Alexandrova, Pencho Beykov, Dobrin Vassilev, Marko Jukić, Črtomir Podlipnik
Biotechnology & Biotechnological Equipment.2021; 35(1): 74. CrossRef - Comprehensive analysis of COVID-19 during pregnancy
Kathryn M. Moore, Mehul S. Suthar
Biochemical and Biophysical Research Communication.2021; 538: 180. CrossRef - Innate Immunity Plays a Key Role in Controlling Viral Load in COVID-19: Mechanistic Insights from a Whole-Body Infection Dynamics Model
Prashant Dogra, Javier Ruiz-Ramírez, Kavya Sinha, Joseph D. Butner, Maria J. Peláez, Manmeet Rawat, Venkata K. Yellepeddi, Renata Pasqualini, Wadih Arap, H. Dirk Sostman, Vittorio Cristini, Zhihui Wang
ACS Pharmacology & Translational Science.2021; 4(1): 248. CrossRef - Transmission of SARS-CoV-2: A Review of Viral, Host, and Environmental Factors
Eric A. Meyerowitz, Aaron Richterman, Rajesh T. Gandhi, Paul E. Sax
Annals of Internal Medicine.2021; 174(1): 69. CrossRef - CRISPR-based detection of SARS-CoV-2: A review from sample to result
Reza Nouri, Zifan Tang, Ming Dong, Tianyi Liu, Aneesh Kshirsagar, Weihua Guan
Biosensors and Bioelectronics.2021; 178: 113012. CrossRef - Evaluation of two fluorescence immunoassays for the rapid detection of SARS-CoV-2 antigen—new tool to detect infective COVID-19 patients
Lorena Porte, Paulette Legarraga, Mirentxu Iruretagoyena, Valeska Vollrath, Gabriel Pizarro, Jose Munita, Rafael Araos, Thomas Weitzel
PeerJ.2021; 9: e10801. CrossRef - Rapid detection of SARS-CoV-2, replicating or non-replicating, using RT-PCR
Ming Liao, Jianmin Wu, Manman Dai, Huanan Li, Nan Yan, Runyu Yuan, Chungen Pan
International Journal of Infectious Diseases.2021; 104: 471. CrossRef - A review on human body fluids for the diagnosis of viral infections: scope for rapid detection of COVID-19
Sphurti S Adigal, Nidheesh V Rayaroth, Reena V John, Keerthilatha M Pai, Sulatha Bhandari, Aswini Kumar Mohapatra, Jijo Lukose, Ajeetkumar Patil, Aseefhali Bankapur, Santhosh Chidangil
Expert Review of Molecular Diagnostics.2021; 21(1): 31. CrossRef - COVID-19 (SARS-CoV-2) outbreak monitoring using wastewater-based epidemiology in Qatar
Jayaprakash Saththasivam, Shimaa S. El-Malah, Tricia A. Gomez, Khadeeja A. Jabbar, Reshma Remanan, Arun K. Krishnankutty, Oluwaseun Ogunbiyi, Kashif Rasool, Sahel Ashhab, Sergey Rashkeev, Meryem Bensaad, Ayeda A. Ahmed, Yasmin A. Mohamoud, Joel A. Malek,
Science of The Total Environment.2021; 774: 145608. CrossRef - A critical review on SARS-CoV-2 infectivity in water and wastewater. What do we know?
Alexandre Giacobbo, Marco Antônio Siqueira Rodrigues, Jane Zoppas Ferreira, Andréa Moura Bernardes, Maria Norberta de Pinho
Science of The Total Environment.2021; 774: 145721. CrossRef - Uncertainties in estimating SARS-CoV-2 prevalence by wastewater-based epidemiology
Xuan Li, Shuxin Zhang, Jiahua Shi, Stephen P. Luby, Guangming Jiang
Chemical Engineering Journal.2021; 415: 129039. CrossRef - Re-estimation of basic reproduction number of COVID-19 based on the epidemic curve by symptom onset date
K. Hong, S. J. Yum, J. H. Kim, B. C. Chun
Epidemiology and Infection.2021;[Epub] CrossRef - Implications of inadequate water and sanitation infrastructure for community spread of COVID-19 in remote Alaskan communities
Laura Eichelberger, Subhabrata Dev, Tricia Howe, David L. Barnes, Eric Bortz, Brandon R. Briggs, Patricia Cochran, Aaron D. Dotson, Devin M. Drown, Micah B. Hahn, Kaitlin Mattos, Srijan Aggarwal
Science of The Total Environment.2021; 776: 145842. CrossRef - Validation of a new automated chemiluminescent anti-SARS-CoV-2 IgM and IgG antibody assay system detecting both N and S proteins in Japan
Rin Yokoyama, Makoto Kurano, Yoshifumi Morita, Takuya Shimura, Yuki Nakano, Chungen Qian, Fuzhen Xia, Fan He, Yoshiro Kishi, Jun Okada, Naoyuki Yoshikawa, Yutaka Nagura, Hitoshi Okazaki, Kyoji Moriya, Yasuyuki Seto, Tatsuhiko Kodama, Yutaka Yatomi, Kateri
PLOS ONE.2021; 16(3): e0247711. CrossRef - Sewage as a Possible Transmission Vehicle During a Coronavirus Disease 2019 Outbreak in a Densely Populated Community: Guangzhou, China, April 2020
Jun Yuan, Zongqiu Chen, Chenghua Gong, Hui Liu, Baisheng Li, Kuibiao Li, Xi Chen, Conghui Xu, Qinlong Jing, Guocong Liu, Pengzhe Qin, Yufei Liu, Yi Zhong, Lijuan Huang, Bao-Ping Zhu, Zhicong Yang
Clinical Infectious Diseases.2021; 73(7): e1795. CrossRef - Pathogenesis of coronavirus disease 2019-associated kidney injury
Kelly D. Smith, Shreeram Akilesh
Current Opinion in Nephrology & Hypertension.2021; 30(3): 324. CrossRef - Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor
Jiahao Li, Ding Wu, Yi Yu, Tingxian Li, Kun Li, Meng-Meng Xiao, Yirong Li, Zhi-Yong Zhang, Guo-Jun Zhang
Biosensors and Bioelectronics.2021; 183: 113206. CrossRef - Focused role of nanoparticles against COVID-19: Diagnosis and treatment
Mohammed Ali Dheyab, Pegah Moradi Khaniabadi, Azlan Abdul Aziz, Mahmood S. Jameel, Baharak Mehrdel, Ammar A. Oglat, Hawraa Ali Khaleel
Photodiagnosis and Photodynamic Therapy.2021; 34: 102287. CrossRef - Determining the communicable period of SARS-CoV-2: A rapid review of the literature, March to September 2020
Mina Park, Colleen Pawliuk, Tribesty Nguyen, Amanda Griffitt, Linda Dix-Cooper, Nadia Fourik, Martin Dawes
Eurosurveillance.2021;[Epub] CrossRef - Maternal respiratory SARS-CoV-2 infection in pregnancy is associated with a robust inflammatory response at the maternal-fetal interface
Alice Lu-Culligan, Arun R. Chavan, Pavithra Vijayakumar, Lina Irshaid, Edward M. Courchaine, Kristin M. Milano, Zhonghua Tang, Scott D. Pope, Eric Song, Chantal B.F. Vogels, William J. Lu-Culligan, Katherine H. Campbell, Arnau Casanovas-Massana, Santos Be
Med.2021; 2(5): 591. CrossRef - SARS-CoV-2: Pathogenesis, Molecular Targets and Experimental Models
G. Kanimozhi, B. Pradhapsingh, Charan Singh Pawar, Haseeb A. Khan, Salman H. Alrokayan, N. Rajendra Prasad
Frontiers in Pharmacology.2021;[Epub] CrossRef - Duration of culturable SARS-CoV-2 within different specimens among mild and severe COVID-19 patients: A longitudinal study
Yae Jee Baek, Youn-Jung Lee, Jae Sun Yoon, Yujin Sohn, Yunsuk Cho, Moo Hyun Kim, Jong Hoon Hyun, Eun Hwa Lee, Ki Hyun Lee, Jung Ho Kim, Jin Young Ahn, Su Jin Jeong, Nam Su Ku, Joon-Sup Yeom, Jun Yong Choi
Journal of Infection.2021; 83(1): e29. CrossRef - Tools and Techniques for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)/COVID-19 Detection
Seyed Hamid Safiabadi Tali, Jason J. LeBlanc, Zubi Sadiq, Oyejide Damilola Oyewunmi, Carolina Camargo, Bahareh Nikpour, Narges Armanfard, Selena M. Sagan, Sana Jahanshahi-Anbuhi
Clinical Microbiology Reviews.2021;[Epub] CrossRef - The risk of transmitting the coronavirus to the perioperative team through aerosols produced in the operating room bathrooms
Mahdieh Soleimani, Amirmohammad Merajikhah, Atefeh Beigi-khoozani
Perioperative Care and Operating Room Management.2021; 24: 100179. CrossRef - Monitoring the presence and persistence of SARS-CoV-2 in water-food-environmental compartments: State of the knowledge and research needs
Bashir Adelodun, Fidelis Odedishemi Ajibade, AbdulGafar Olatunji Tiamiyu, Nathaniel Azubuike Nwogwu, Rahmat Gbemisola Ibrahim, Pankaj Kumar, Vinod Kumar, Golden Odey, Krishna Kumar Yadav, Afzal Husain Khan, Marina M.S. Cabral-Pinto, Kola Yusuff Kareem, Ha
Environmental Research.2021; 200: 111373. CrossRef - Nucleic Acid Testing of SARS-CoV-2
Hee Min Yoo, Il-Hwan Kim, Seil Kim
International Journal of Molecular Sciences.2021; 22(11): 6150. CrossRef - What’s in the Pipeline? Evidence on the Transmission of SARS-CoV-2 via Building Wastewater Plumbing Systems
Thomas Dight, Michael Gormley
Frontiers in Built Environment.2021;[Epub] CrossRef - Kidney transplantation from a SARS‐CoV‐2‐positive donor for the recipients with immunity after COVID‐19
Lina Puodziukaite, Mindaugas Serpytis, Aurelija Kundrotaite, Jurate Sipylaite, Marius Miglinas, Marta Monika Janusaite, Egle Asakiene, Erika Milisauskaite, Arunas Zelvys, Oleg Aliancevic, Ligita Jancoriene
Transplant Infectious Disease.2021;[Epub] CrossRef - Evidence of SARS-CoV-2 Infection in Cells, Tissues, and Organs and the Risk of Transmission Through Transplantation
Amaury Gaussen, Laura Hornby, Gary Rockl, Sheila O’Brien, Gilles Delage, Ruth Sapir-Pichhadze, Steven J. Drews, Matthew J. Weiss, Antoine Lewin
Transplantation.2021; 105(7): 1405. CrossRef - Evaluation of SARS-CoV-2 in the Vaginal Secretions of Women with COVID-19: A Prospective Study
Elad Barber, Michal Kovo, Sophia Leytes, Ron Sagiv, Eran Weiner, Orna Schwartz, Margarita Mashavi, Keren Holtzman, Jacob Bar, Anat Engel, Shimon Ginath
Journal of Clinical Medicine.2021; 10(12): 2735. CrossRef - Detection of SARS-CoV-2 RNA in serum is associated with increased mortality risk in hospitalized COVID-19 patients
Diego A. Rodríguez-Serrano, Emilia Roy-Vallejo, Nelly D. Zurita Cruz, Alexandra Martín Ramírez, Sebastián C. Rodríguez-García, Nuria Arevalillo-Fernández, José María Galván-Román, Leticia Fontán García-Rodrigo, Lorena Vega-Piris, Marta Chicot Llano, David
Scientific Reports.2021;[Epub] CrossRef - Needle Stick Injury From a COVID-19 Patient—Fear It or Forget It?
Vishakh C. Keri, Parul Kodan, Anubhav Gupta, Pankaj Jorwal
Journal of Bioethical Inquiry.2021; 18(3): 377. CrossRef - Detection of three pandemic causing coronaviruses from non-respiratory samples: systematic review and meta-analysis
Chandan Mishra, Suneeta Meena, Jitendra Kumar Meena, Suman Tiwari, Purva Mathur
Scientific Reports.2021;[Epub] CrossRef - Sewage Systems Surveillance for SARS-CoV-2: Identification of Knowledge Gaps, Emerging Threats, and Future Research Needs
Fatemeh Amereh, Masoud Negahban-Azar, Siavash Isazadeh, Hossein Dabiri, Najmeh Masihi, Mahsa Jahangiri-rad, Mohammad Rafiee
Pathogens.2021; 10(8): 946. CrossRef - Probiotics and Coronavirus disease 2019: think about the link
Suresh Kumar Angurana, Arun Bansal
British Journal of Nutrition.2021; 126(10): 1564. CrossRef - Role of Nucleocapsid Protein Antigen Detection for Safe End of Isolation of SARS-CoV-2 Infected Patients with Long Persistence of Viral RNA in Respiratory Samples
Antonella Mencacci, Alessio Gili, Anna Gidari, Elisabetta Schiaroli, Carla Russo, Elio Cenci, Barbara Camilloni, Alessandro Graziani, Arduino Melelli-Roia, Daniela Francisci, Fabrizio Stracci
Journal of Clinical Medicine.2021; 10(18): 4037. CrossRef - Designing Short Peptides to Block the Interaction of SARS-CoV-2 and Human ACE2 for COVID-19 Therapeutics
Abdul Basit, Asad Mustafa Karim, Muhammad Asif, Tanveer Ali, Jung Hun Lee, Jeong Ho Jeon, Shafiq ur Rehman, Sang Hee Lee
Frontiers in Pharmacology.2021;[Epub] CrossRef - Tracking SARS-CoV-2: Novel Trends and Diagnostic Strategies
Linda P. Guaman-Bautista, Erick Moreta-Urbano, Claudia G. Oña-Arias, Marbel Torres-Arias, Nikolaos C. Kyriakidis, Koray Malcı, Nestor Jonguitud-Borrego, Leonardo Rios-Solis, Espiridion Ramos-Martinez, Andrés López-Cortés, Carlos Barba-Ostria
Diagnostics.2021; 11(11): 1981. CrossRef - Probable Case of Vertical Transmission of SARS-CoV-2 in a Newborn in Mexico
Marcela Mendoza-Hernández, Ivette Huerta-Niño de Rivera, María Yoldi-Negrete, Pamela Saviñon-Tejeda, Rafael Franco-Cendejas, Luis Esaú López-Jácome, Iñaki Navarro-Castellanos
Neonatology.2021; 118(3): 364. CrossRef - Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution melting
Beatriz Iandra da Silva Ferreira, Natália Lins da Silva-Gomes, Wagner Luis da Costa Nunes Pimentel Coelho, Vanessa Duarte da Costa, Vanessa Cristine de Souza Carneiro, Rafael Lopes Kader, Marisa Pimentel Amaro, Lívia Melo Villar, Fábio Miyajima, Soniza Vi
PLOS ONE.2021; 16(11): e0260087. CrossRef - Cross-validation of SARS-CoV-2 responses in kidney organoids and clinical populations
Louisa Helms, Silvia Marchiano, Ian B. Stanaway, Tien-Ying Hsiang, Benjamin A. Juliar, Shally Saini, Yan Ting Zhao, Akshita Khanna, Rajasree Menon, Fadhl Alakwaa, Carmen Mikacenic, Eric D. Morrell, Mark M. Wurfel, Matthias Kretzler, Jennifer L. Harder, Ch
JCI Insight.2021;[Epub] CrossRef - COVID-19 and kidney involvement – A systematic review
Guang Yang, Yang Liu, Jiebin Hou, Qingli Cheng
Integrative Medicine in Nephrology and Andrology.2021; 8(1): 4. CrossRef - Viable SARS-CoV-2 in various specimens from COVID-19 patients
Hye Won Jeong, Se-Mi Kim, Hee-Sung Kim, Young-Il Kim, Jun Hyoung Kim, Jun Yeon Cho, Sun-hyung Kim, Hyeran Kang, Seong-Gyu Kim, Su-Jin Park, Eun-Ha Kim, Young Ki Choi
Clinical Microbiology and Infection.2020; 26(11): 1520. CrossRef - Risk of transmission of severe acute respiratory syndrome coronavirus 2 by transfusion: A literature review
Jean‐François Leblanc, Marc Germain, Gilles Delage, Sheila OʼBrien, Steven J. Drews, Antoine Lewin
Transfusion.2020; 60(12): 3046. CrossRef - Potential sources, modes of transmission and effectiveness of prevention measures against SARS-CoV-2
G. Kampf, Y. Brüggemann, H.E.J. Kaba, J. Steinmann, S. Pfaender, S. Scheithauer, E. Steinmann
Journal of Hospital Infection.2020; 106(4): 678. CrossRef - Does SARS-CoV-2 Infect the Kidney?
Shaza Khan, Lihe Chen, Chin-Rang Yang, Viswanathan Raghuram, Syed J. Khundmiri, Mark A. Knepper
Journal of the American Society of Nephrology.2020; 31(12): 2746. CrossRef - Les Coronavirus humains
Michel Segondy
Revue Francophone des Laboratoires.2020; 2020(526): 32. CrossRef - In reply: an example of the evolution of evidence in a new disease
Benjamin Condon, Thomas Whish-Wilson, Niall F. Davis, Nathan Lawrentschuk
Future Oncology.2020; 16(35): 2903. CrossRef - Wastewater-Based Epidemiology (WBE) and Viral Detection in Polluted Surface Water: A Valuable Tool for COVID-19 Surveillance—A Brief Review
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- Identification of Coronavirus Isolated from a Patient in Korea with COVID-19
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Jeong-Min Kim, Yoon-Seok Chung, Hye Jun Jo, Nam-Joo Lee, Mi Seon Kim, Sang Hee Woo, Sehee Park, Jee Woong Kim, Heui Man Kim, Myung-Guk Han
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Osong Public Health Res Perspect. 2020;11(1):3-7. Published online February 28, 2020
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DOI: https://doi.org/10.24171/j.phrp.2020.11.1.02
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Abstract
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Objectives
Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.
Methods
Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.
Results
The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.
Conclusion
SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.
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