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Mehrdad Gholami 2 Articles
Analysis of Resistance to Macrolide–Lincosamide–Streptogramin B Among mecA-Positive Staphylococcus Aureus Isolates
Mahmoud Khodabandeh, Mohsen Mohammadi, Mohammad Reza Abdolsalehi, Azadeh Alvandimanesh, Mehrdad Gholami, Meysam Hasannejad Bibalan, Abazar Pournajaf, Ramin Kafshgari, Ramazan Rajabnia
Osong Public Health Res Perspect. 2019;10(1):25-31.   Published online February 28, 2019
DOI: https://doi.org/10.24171/j.phrp.2019.10.1.06
  • 3,864 View
  • 165 Download
  • 18 Citations
AbstractAbstract PDF
Objectives

Genetic determinants conferring resistance to macrolide, lincosamide, and streptogramin B (MLSB) via ribosomal modification such as, erm, msrA/B and ereA/B genes are distributed in bacteria. The main goals of this work were to evaluate the dissemination of MLSB resistance phenotypes and genotypes in methicillin-resistant Staphylococcus aureus (MRSA) isolates collected from clinical samples.

Methods

A total of 106 MRSA isolates were studied. Isolates were recovered from 3 hospitals in Tehran between May 2016 to July 2017. The prevalence of MLSB-resistant strains were determined by D-test, and then M-PCR was performed to identify genes encoding resistance to macrolides, lincosamides, and streptogramins in the tested isolates.

Results

The frequency of constitutive resistance MLSB, inducible resistance MLSB and MSB resistance were 56.2%, 22.9%, and 16.6%, respectively. Of 11 isolates with the inducible resistance MLSB phenotype, ermC, ermB, ermA and ereA were positive in 81.8%, 63.6%, 54.5% and 18.2% of these isolates, respectively. In isolates with the constitutive resistance MLSB phenotype, the prevalence of ermA, ermB, ermC, msrA, msrB, ereA and ereB were 25.9%, 18.5%, 44.4%, 0.0%, 0.0%, 11.1% and 0.0%, respectively.

Conclusion

Clindamycin is commonly administered in severe MRSA infections depending upon the antimicrobial susceptibility findings. This study showed that the D-test should be used as an obligatory method in routine disk diffusion assay to detect inducible clindamycin resistance in MRSA so that effective antibiotic treatment can be provided.

Citations

Citations to this article as recorded by  
  • Characterization of oxacillin-resistant Staphylococcus lugdunensis isolated from sterile body fluids in a medical center in Taiwan: A 12-year longitudinal epidemiological study
    Shih-Cheng Chang, Jazon Harl Hidrosollo, Lee-Chung Lin, Yu-Hsiang Ou, Cheng-Yen Kao, Jang-Jih Lu
    Journal of Microbiology, Immunology and Infection.2022;[Epub]     CrossRef
  • New update on molecular diversity of clinical Staphylococcus aureus isolates in Iran: antimicrobial resistance, adhesion and virulence factors, biofilm formation and SCCmec typing
    Mahtab Tabandeh, Hami Kaboosi, Mojtaba Taghizadeh Armaki, Abazar Pournajaf, Fatemeh Peyravii Ghadikolaii
    Molecular Biology Reports.2022; 49(4): 3099.     CrossRef
  • Inducible Clindamycin-Resistant Staphylococcus aureus Strains in Africa: A Systematic Review
    Muluneh Assefa, Faham Khamesipour
    International Journal of Microbiology.2022; 2022: 1.     CrossRef
  • Genetic Diversity and Virulence Profile of Methicillin and Inducible Clindamycin-Resistant Staphylococcus aureus Isolates in Western Algeria
    Zahoua Mentfakh Laceb, Seydina M. Diene, Rym Lalaoui, Mabrouk Kihal, Fella Hamaidi Chergui, Jean-Marc Rolain, Linda Hadjadj
    Antibiotics.2022; 11(7): 971.     CrossRef
  • Molecular typing, biofilm formation, and analysis of adhesion factors in Staphylococcus aureus strains isolated from urinary tract infections
    Masoumeh Navidinia, Anis Mohammadi, Reza Arjmand, Masoud Dadashi, Mehdi Goudarzi
    Gene Reports.2021; 22: 101008.     CrossRef
  • Molecular characterization of invasive Staphylococcus aureus strains isolated from patients with diabetes in Iran: USA300 emerges as the major type
    Zahra Tayebi, Maryam Fazeli, Ali Hashemi, Saeed Abdi, Masoud Dadashi, Mohammad Javad Nasiri, Mehdi Goudarzi
    Infection, Genetics and Evolution.2021; 87: 104679.     CrossRef
  • Predominance of PVL-negative community-associated methicillin-resistant Staphylococcus aureus sequence type 8 in newly diagnosed HIV-infected adults, Tanzania
    Joel Manyahi, Sabrina J. Moyo, Said Aboud, Nina Langeland, Bjørn Blomberg
    European Journal of Clinical Microbiology & Infect.2021; 40(7): 1477.     CrossRef
  • Molecular characterization of Staphylococcus aureus strains isolated from hospitalized patients based on coagulase gene polymorphism analysis: High frequency of vancomycin-intermediate S. aureus and the emergence of coagulase type II in Iran
    Malihe Soltani, Bahareh Hajikhani, Samira Zamani, Mehrdad Haghighi, Ali Hashemi, Mohammad Javad Nasiri, Masoud Dadashi, Behzad Pourhossein, Mehdi Goudarzi
    Gene Reports.2021; 23: 101078.     CrossRef
  • Inducible clindamycin resistance among clinical Staphylococcus aureus strains in Iran: A contemporaneous systematic review and meta-analysis
    Mojtaba Memariani, Hamed Memariani, Hamideh Moravvej
    Gene Reports.2021; 23: 101104.     CrossRef
  • Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients
    Amr S. Bishr, Salma M. Abdelaziz, Ibrahim S. Yahia, Mahmoud A. Yassien, Nadia A. Hassouna, Khaled M. Aboshanab
    Biology.2021; 10(7): 624.     CrossRef
  • Relationship between MLSB resistance and the prevalent virulence genotypes among Bulgarian Staphylococcus aureus isolates
    Virna-Maria Tsitou, Ivan Mitov, Raina Gergova
    Acta Microbiologica et Immunologica Hungarica.2021; 68(1): 55.     CrossRef
  • Occurrence, Antibiotic Resistance, Virulence Factors, and Genetic Diversity of Bacillus spp. from Public Hospital Environments in South Africa
    Zamile N. Mbhele, Christiana O. Shobo, Daniel G. Amoako, Oliver T. Zishiri, Linda A. Bester
    Microbial Drug Resistance.2021; 27(12): 1692.     CrossRef
  • Bacterial Antibiotic Resistance: The Most Critical Pathogens
    Giuseppe Mancuso, Angelina Midiri, Elisabetta Gerace, Carmelo Biondo
    Pathogens.2021; 10(10): 1310.     CrossRef
  • Mechanisms of Resistance to Macrolide Antibiotics among Staphylococcus aureus
    Maria Miklasińska-Majdanik
    Antibiotics.2021; 10(11): 1406.     CrossRef
  • Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Human Bloodstream Infections: Detection of MLSB Resistance
    Vanessa Silva, Sara Hermenegildo, Catarina Ferreira, Célia M. Manaia, Rosa Capita, Carlos Alonso-Calleja, Isabel Carvalho, José Eduardo Pereira, Luis Maltez, José L. Capelo, Gilberto Igrejas, Patrícia Poeta
    Antibiotics.2020; 9(7): 375.     CrossRef
  • Genetic analysis of methicillin‐susceptible Staphylococcus aureus clinical isolates: High prevalence of multidrug‐resistant ST239 with strong biofilm‐production ability
    Hossein Goudarzi, Mehdi Goudarzi, Fattaneh Sabzehali, Maryam Fazeli, Alireza Salimi Chirani
    Journal of Clinical Laboratory Analysis.2020;[Epub]     CrossRef
  • Emergence and spread of coagulase type III and staphylococcal cassette chromosome mec type IV among mupirocin-resistant Staphylococcus aureus isolated from wound infections
    Mirmohammad Miri, Maryam Fazeli, Anahita Amirpour, Mohammad Javad Nasiri, Ramin Pouriran, Mehdi Goudarzi
    Gene Reports.2020; 21: 100858.     CrossRef
  • Resistance profile to antimicrobials agents in methicillin-resistant Staphylococcus aureus isolated from hospitals in South Brazil between 2014-2019
    Adriana Medianeira Rossato, Muriel Primon-Barros, Lisiane da Luz Rocha, Keli Cristine Reiter, Cícero Armídio Gomes Dias, Pedro Alves d’Azevedo
    Revista da Sociedade Brasileira de Medicina Tropic.2020;[Epub]     CrossRef
The Effect of Lactobacillus acidophilus PTCC 1643 on Cultured Intestinal Epithelial Cells Infected with Salmonella enterica serovar Enteritidis
Mona Moshiri, Mohammad Mehdi Soltan Dallal, Farhad Rezaei, Masoumeh Douraghi, Laleh Sharifi, Zahra Noroozbabaei, Mehrdad Gholami, Abbas Mirshafiey
Osong Public Health Res Perspect. 2017;8(1):54-60.   Published online February 28, 2017
DOI: https://doi.org/10.24171/j.phrp.2017.8.1.07
  • 2,736 View
  • 20 Download
  • 8 Citations
AbstractAbstract PDF
Objectives

Gastrointestinal disorders caused by Salmonella enterica serovar Enteritidis (SesE) are a significant health problem around the globe. Probiotic bacteria have been shown to have positive effects on the immune responses. Lactobacillus acidophilus was examined for its capability to influence the innate immune response of HT29 intestinal epithelial cells towards SesE. The purpose of this work was to assess the effect of L. acidophilus PTCC 1643 on cultured intestinal epithelial cells infected with SesE.

Methods

HT29 cells were cultured in Roswell Park Memorial Institute medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. The cells were treated with L. acidophilus PTCC 1643 after or before challenge with SesE. At 2 and 4 hours post-infection, we measured changes in the expression levels of TLR2 and TLR4 via real-time polymerase chain reaction.

Results

Treatment with L. acidophilus inhibited SesE-induced increases in TLR2 and TLR4 expression in the infected HT29 cells. Moreover, the expression of TLR2 and TLR4 in cells that were pretreated with L. acidophilus and then infected with SesE was significantly higher than that in cells infected with SesE without pretreatment. Taken together, the results indicated that L. acidophilus had an anti-inflammatory effect and modulated the innate immune response to SesE by influencing TLR2 and TLR4 expression.

Conclusion

Our findings suggested that L. acidophilus PTCC 1643 was able to suppress inflammation caused by SesE infection in HT29 cells and reduce TLR2 and TLR4 expression. Additional in vivo and in vitro studies are required to further elucidate the mechanisms underlying this anti-inflammatory effect.

Citations

Citations to this article as recorded by  
  • Lactobacillus acidophilus ATCC 4356 Exopolysaccharides Suppresses Mediators of Inflammation through the Inhibition of TLR2/STAT-3/P38-MAPK Pathway in DEN-Induced Hepatocarcinogenesis in Rats
    Ola M. S. Khedr, Sawsan M. El-Sonbaty, Fatma S. M. Moawed, Eman I. Kandil, Basma E. Abdel-Maksoud
    Nutrition and Cancer.2022; 74(3): 1037.     CrossRef
  • Osmoporation is a versatile technique to encapsulate fisetin using the probiotic bacteria Lactobacillus acidophilus
    Eduardo Wagner Vasconcelos de Andrade, Sebastien Dupont, Laurent Beney, Roberta Targino Hoskin, Márcia Regina da Silva Pedrini
    Applied Microbiology and Biotechnology.2022; 106(3): 1031.     CrossRef
  • The Game for Three: Salmonella–Host–Microbiota Interaction Models
    Krzysztof Grzymajlo
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • The Functional Roles of Lactobacillus acidophilus in Different Physiological and Pathological Processes
    Huijuan Gao, Xin Li, Xiatian Chen, Deng Hai, Chuang Wei, Lei Zhang, Peifeng Li
    Journal of Microbiology and Biotechnology.2022; 32(10): 1226.     CrossRef
  • Improving bioactive properties of peach juice using Lactobacillus strains fermentation: Antagonistic and anti-adhesion effects, anti-inflammatory and antioxidant properties, and Maillard reaction inhibition
    Seyed Mohammad Bagher Hashemi, Dornoush Jafarpour, Mohammad Jouki
    Food Chemistry.2021; 365: 130501.     CrossRef
  • The immune regulatory role of Lactobacillus acidophilus: An updated meta-analysis of randomized controlled trials
    Wei Zhao, Yangshuo Liu, Lai-Yu Kwok, Tiequan Cai, Wenyi Zhang
    Food Bioscience.2020; 36: 100656.     CrossRef
  • Gene expression changes as predictors of the immune-modulatory effects of probiotics: Towards a better understanding of strain-disease specific interactions
    Frida Gorreja
    NFS Journal.2019; 14-15: 1.     CrossRef
  • A review on anti-adhesion therapies of bacterial diseases
    Arezoo Asadi, Shabnam Razavi, Malihe Talebi, Mehrdad Gholami
    Infection.2019; 47(1): 13.     CrossRef

PHRP : Osong Public Health and Research Perspectives