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Masoumeh Douraghi 2 Articles
The Effect of Lactobacillus acidophilus PTCC 1643 on Cultured Intestinal Epithelial Cells Infected with Salmonella enterica serovar Enteritidis
Mona Moshiri, Mohammad Mehdi Soltan Dallal, Farhad Rezaei, Masoumeh Douraghi, Laleh Sharifi, Zahra Noroozbabaei, Mehrdad Gholami, Abbas Mirshafiey
Osong Public Health Res Perspect. 2017;8(1):54-60.   Published online February 28, 2017
DOI: https://doi.org/10.24171/j.phrp.2017.8.1.07
  • 2,765 View
  • 20 Download
  • 8 Citations
AbstractAbstract PDF
Objectives

Gastrointestinal disorders caused by Salmonella enterica serovar Enteritidis (SesE) are a significant health problem around the globe. Probiotic bacteria have been shown to have positive effects on the immune responses. Lactobacillus acidophilus was examined for its capability to influence the innate immune response of HT29 intestinal epithelial cells towards SesE. The purpose of this work was to assess the effect of L. acidophilus PTCC 1643 on cultured intestinal epithelial cells infected with SesE.

Methods

HT29 cells were cultured in Roswell Park Memorial Institute medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. The cells were treated with L. acidophilus PTCC 1643 after or before challenge with SesE. At 2 and 4 hours post-infection, we measured changes in the expression levels of TLR2 and TLR4 via real-time polymerase chain reaction.

Results

Treatment with L. acidophilus inhibited SesE-induced increases in TLR2 and TLR4 expression in the infected HT29 cells. Moreover, the expression of TLR2 and TLR4 in cells that were pretreated with L. acidophilus and then infected with SesE was significantly higher than that in cells infected with SesE without pretreatment. Taken together, the results indicated that L. acidophilus had an anti-inflammatory effect and modulated the innate immune response to SesE by influencing TLR2 and TLR4 expression.

Conclusion

Our findings suggested that L. acidophilus PTCC 1643 was able to suppress inflammation caused by SesE infection in HT29 cells and reduce TLR2 and TLR4 expression. Additional in vivo and in vitro studies are required to further elucidate the mechanisms underlying this anti-inflammatory effect.

Citations

Citations to this article as recorded by  
  • Lactobacillus acidophilus ATCC 4356 Exopolysaccharides Suppresses Mediators of Inflammation through the Inhibition of TLR2/STAT-3/P38-MAPK Pathway in DEN-Induced Hepatocarcinogenesis in Rats
    Ola M. S. Khedr, Sawsan M. El-Sonbaty, Fatma S. M. Moawed, Eman I. Kandil, Basma E. Abdel-Maksoud
    Nutrition and Cancer.2022; 74(3): 1037.     CrossRef
  • Osmoporation is a versatile technique to encapsulate fisetin using the probiotic bacteria Lactobacillus acidophilus
    Eduardo Wagner Vasconcelos de Andrade, Sebastien Dupont, Laurent Beney, Roberta Targino Hoskin, Márcia Regina da Silva Pedrini
    Applied Microbiology and Biotechnology.2022; 106(3): 1031.     CrossRef
  • The Game for Three: Salmonella–Host–Microbiota Interaction Models
    Krzysztof Grzymajlo
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • The Functional Roles of Lactobacillus acidophilus in Different Physiological and Pathological Processes
    Huijuan Gao, Xin Li, Xiatian Chen, Deng Hai, Chuang Wei, Lei Zhang, Peifeng Li
    Journal of Microbiology and Biotechnology.2022; 32(10): 1226.     CrossRef
  • Improving bioactive properties of peach juice using Lactobacillus strains fermentation: Antagonistic and anti-adhesion effects, anti-inflammatory and antioxidant properties, and Maillard reaction inhibition
    Seyed Mohammad Bagher Hashemi, Dornoush Jafarpour, Mohammad Jouki
    Food Chemistry.2021; 365: 130501.     CrossRef
  • The immune regulatory role of Lactobacillus acidophilus: An updated meta-analysis of randomized controlled trials
    Wei Zhao, Yangshuo Liu, Lai-Yu Kwok, Tiequan Cai, Wenyi Zhang
    Food Bioscience.2020; 36: 100656.     CrossRef
  • Gene expression changes as predictors of the immune-modulatory effects of probiotics: Towards a better understanding of strain-disease specific interactions
    Frida Gorreja
    NFS Journal.2019; 14-15: 1.     CrossRef
  • A review on anti-adhesion therapies of bacterial diseases
    Arezoo Asadi, Shabnam Razavi, Malihe Talebi, Mehrdad Gholami
    Infection.2019; 47(1): 13.     CrossRef
Identification of Klebsiella pneumoniae Carbapenemase-producing Klebsiella oxytoca in Clinical Isolates in Tehran Hospitals, Iran by Chromogenic Medium and Molecular Methods
Majid Validi, Mohammad Mehdi Soltan Dallal, Masoumeh Douraghi, Jalil Fallah Mehrabadi, Abbas Rahimi Foroushani
Osong Public Health Res Perspect. 2016;7(5):301-306.   Published online October 31, 2016
DOI: https://doi.org/10.1016/j.phrp.2016.08.006
  • 1,837 View
  • 25 Download
  • 8 Citations
AbstractAbstract PDF
Objectives
Production of carbapenemase, especially Klebsiella pneumoniae carbapenemases (KPC), is one of the antibiotic resistance mechanisms of Enterobacteriaceae such as Klebsiella oxytoca. This study aimed to investigate and identify KPC-producing K. oxytoca isolates using molecular and phenotypic methods.
Methods
A total of 75 isolates of K. oxytoca were isolated from various clinical samples, and were verified as K. oxytoca after performing standard microbiological tests and using a polymerase chain reaction (PCR) method. An antibiotic susceptibility test was performed using a disc diffusion method according to the Clinical and Laboratory Standards Institute guidelines. CHROMagar KPC chromogenic culture media was used to examine and confirm the production of the carbapenemase enzyme in K. oxytoca isolates; in addition, PCR was used to evaluate the presence of blaKPC gene in K. oxytoca strains.
Results
Of a total of 75 K. oxytoca isolates, one multidrug resistant strain was isolated from the urine of a hospitalized woman. This strain was examined to assess its ability to produce carbapenemase enzyme; it produced a colony with a blue metallic color on the CHROMagar KPC chromogenic culture media. In addition, the blaKPC gene was confirmed by PCR. After sequencing, it was confirmed and deposited in GenBank.
Conclusion
To date, many cases of KPC-producing Enterobacteriaceae, in particular K. pneumoniae, have been reported in different countries; there are also some reports on the identification of KPC-producing K. oxytoca. Therefore, to prevent the outbreak of nosocomial infections, the early detection, control, and prevention of the spread of these strains are of great importance.

Citations

Citations to this article as recorded by  
  • Klebsiella oxytoca Complex: Update on Taxonomy, Antimicrobial Resistance, and Virulence
    Jing Yang, Haiyan Long, Ya Hu, Yu Feng, Alan McNally, Zhiyong Zong
    Clinical Microbiology Reviews.2022;[Epub]     CrossRef
  • Development and comparison of immunochromatographic strips with four nanomaterial labels: Colloidal gold, new colloidal gold, multi-branched gold nanoflowers and Luminol-reduced Au nanoparticles for visual detection of Vibrio parahaemolyticus in seafood
    Meijiao Wu, Youxue Wu, Cheng Liu, Yachen Tian, Shuiqin Fang, Hao Yang, Bin Li, Qing Liu
    Aquaculture.2021; 539: 736563.     CrossRef
  • Variation in Accessory Genes Within the Klebsiella oxytoca Species Complex Delineates Monophyletic Members and Simplifies Coherent Genotyping
    Amar Cosic, Eva Leitner, Christian Petternel, Herbert Galler, Franz F. Reinthaler, Kathrin A. Herzog-Obereder, Elisabeth Tatscher, Sandra Raffl, Gebhard Feierl, Christoph Högenauer, Ellen L. Zechner, Sabine Kienesberger
    Frontiers in Microbiology.2021;[Epub]     CrossRef
  • Multidrug-Resistant Bacteria and Alternative Methods to Control Them: An Overview
    Roberto Vivas, Ana Andréa Teixeira Barbosa, Silvio Santana Dolabela, Sona Jain
    Microbial Drug Resistance.2019; 25(6): 890.     CrossRef
  • Molecular typing of cytotoxin-producing Klebsiella oxytoca isolates by 16S-23S internal transcribed spacer PCR
    M.M. Soltan Dallal, M. Validi, M. Douraghi, B. Bakhshi
    New Microbes and New Infections.2019; 30: 100545.     CrossRef
  • Determination of antibiotic resistance and minimum inhibitory concentration of meropenem and imipenem growth in Klebsiella strains isolated from urinary tract infection in Shahrekord educational hospitals
    Farshad Kakian, Behnam Zamzad, Abolfazl Gholipour, Kiarash Zamanzad
    Journal of Shahrekord University of Medical Scienc.2019; 21(2): 80.     CrossRef
  • Evaluation the cytotoxic effect of cytotoxin-producing Klebsiella oxytoca isolates on the HEp-2 cell line by MTT assay
    Mohammad Mehdi Soltan-Dallal, Majid Validi, Masoumeh Douraghi, Jalil Fallah-Mehrabadi, Leila Lormohammadi
    Microbial Pathogenesis.2017; 113: 416.     CrossRef
  • Outbreak by Hypermucoviscous Klebsiella pneumoniae ST11 Isolates with Carbapenem Resistance in a Tertiary Hospital in China
    Lingling Zhan, Shanshan Wang, Yinjuan Guo, Ye Jin, Jingjing Duan, Zhihao Hao, Jingnan Lv, Xiuqin Qi, Longhua Hu, Liang Chen, Barry N. Kreiswirth, Rong Zhang, Jingye Pan, Liangxing Wang, Fangyou Yu
    Frontiers in Cellular and Infection Microbiology.2017;[Epub]     CrossRef

PHRP : Osong Public Health and Research Perspectives