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Maryam Farzi 1 Article
Original Article
Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei
Amir Ghasemi, Sobhan Ghafourian, Sedighe Vafaei, Reza Mohebi, Maryam Farzi, Morovat Taherikalani, Nourkhoda Sadeghifard
Osong Public Health Res Perspect. 2015;6(6):336-340.   Published online December 31, 2015
Objectives In an attempt α-amylase gene from Pyrococcus woesei was amplified and cloned into a pTYB2 vector to generate the recombinant plasmid pTY- α-amylase. Methods Escherichia coli BL21 used as a host and protein expression was applied using IPTG. SDS-PAGE assay demonstrated the 100 kDa protein. Amylolytic activity of proteins produced by transformed E. coli cells was detected by zymography, and the rate of...

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Print ISSN: 2210-9099
Online ISSN: 2233-6052

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