- Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing Escherichia coli in Healthy Children from a Rural Andean Village in Venezuela
-
María Araque, Indira Labrador
-
Osong Public Health Res Perspect. 2018;9(1):9-15. Published online December 31, 2017
-
DOI: https://doi.org/10.24171/j.phrp.2018.9.1.03
-
-
7,198
View
-
52
Download
-
12
Crossref
-
Abstract
PDF
-
Objectives
Antimicrobial resistant extended-spectrum-β-lactamase-producing Enterobacteriaceae (ESBL-PE) have been shown to be present in healthy communities. This study examined healthy children from the rural Andean village of Llano del Hato, Mérida, Venezuela, who have had little or no antibiotic exposure to determine the prevalence of fecal carriage of ESBL-producing Escherichia coli (ESBL-EC).
Methods
A total of 78 fecal samples were collected in healthy children aged from 1 to 5 years. ESBL-EC were selected in MacConkey agar plates with cefotaxime and further confirmed by the VITEK 2 system. ESBL were phenotypically detected and presence of bla genes and their variants were confirmed by molecular assays. Determination of phylogenetic groups was performed by PCR amplification. Risk factors associated with fecal carriage of ESBL-EC-positive isolates were analyzed using standard statistical methods.
Results
Of the 78 children studied, 27 (34.6%) carried ESBL-EC. All strains harbored the blaCTX-M-15 allele. Of these, 8 were co-producers of blaTEM-1, blaTEM-5, blaSHV-5 or blaSHV-12. Co-resistance to aminoglycosides and/or fluoroquinolones was observed in 9 strains. 51.9% of ESBL-EC isolates were classified within phylogroup A. A significant, positive correlation was found between age (≥2.5 – ≤5 years), food consumption patterns and ESBL-EC fecal carriage.
Conclusion
This is the first study describing the high prevalence of fecal carriage of ESBL-EC expressing CTX-M-15- among very young, healthy children from a rural Andean village in Venezuela with scarce antibiotic exposure, underlining the importance of this population as a reservoir.
-
Citations
Citations to this article as recorded by 
- First Report of IMI-2-Producing Enterobacter bugandensis and CTX-M-55-Producing Escherichia coli isolated from Healthy Volunteers in Tunisia
Rym Ben Sallem, Ameni Arfaoui, Afef Najjari, Isabel Carvalho, Abdelmalek Lekired, Hadda-Imen Ouzari, Karim Ben Slama, Alex Wong, Carmen Torres, Naouel Klibi Antibiotics.2023; 12(1): 116. CrossRef - Co-Harboring of Beta-Lactamases and mcr-1 Genes in Escherichia coli and Klebsiella pneumoniae from Healthy Carriers and Backyard Animals in Rural Communities in Ecuador
Carlos Bastidas-Caldes, Emily Cisneros-Vásquez, Antonella Zambrano, Andrea Mosquera-Maza, William Calero-Cáceres, Joaquín Rey, Yoshimasa Yamamoto, Mayumi Yamamoto, Manuel Calvopiña, Jacobus H. de Waard Antibiotics.2023; 12(5): 856. CrossRef - Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae Family of Bacteria from Diarrheal Stool Samples in Northwest Ethiopia
Minichil Worku, Michael Getie, Feleke Moges, Alem Getaneh Mehari, Jayaraman Tharmalingam Interdisciplinary Perspectives on Infectious Disea.2022; 2022: 1. CrossRef - GENOMIC CHARACTERIZATION OF MULTIDRUG-RESISTANT EXTENDED-SPECTRUM β-LACTAMASE–PRODUCING ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE FROM CHIMPANZEES (PAN TROGLODYTES) FROM WILD AND SANCTUARY LOCATIONS IN UGANDA
Sandra L. Bager, Isaac Kakaala, Egle Kudirkiene, Denis K. Byarugaba, John Elmerdahl Olsen Journal of Wildlife Diseases.2022;[Epub] CrossRef - Relevant increase of CTX-M-producing Escherichia coli carriage in school-aged children from rural areas of the Bolivian Chaco in a three-year period
Selene Rebecca Boncompagni, Maria Micieli, Tiziana Di Maggio, Antonia Mantella, Anna Liz Villagrán, Tatiana Briggesth Miranda, Carmen Revollo, Veronica Poma, Herlan Gamboa, Michele Spinicci, Marianne Strohmeyer, Alessandro Bartoloni, Gian Maria Rossolini, International Journal of Infectious Diseases.2022; 121: 126. CrossRef - Comparison of the global prevalence and trend of human intestinal carriage of ESBL-producing Escherichia coli between healthcare and community settings: a systematic review and meta-analysis
Yihienew M. Bezabih, Alemayehu Bezabih, Michel Dion, Eric Batard, Samson Teka, Abiy Obole, Noah Dessalegn, Alelegn Enyew, Anna Roujeinikova, Endalkachew Alamneh, Corinne Mirkazemi, Gregory M. Peterson, Woldesellassie M. Bezabhe JAC-Antimicrobial Resistance.2022;[Epub] CrossRef - Fecal Carriage of Extended-Spectrum β-Lactamases and pAmpC Producing Enterobacterales in an Iranian Community: Prevalence, Risk Factors, Molecular Epidemiology, and Antibiotic Resistance
Nasrin Habibzadeh, Hadi Peeri Doghaheh, Meysam Manouchehri Far, Hossein Alimohammadi Asl, Sohrab Iranpour, Mohsen Arzanlou Microbial Drug Resistance.2022; 28(9): 921. CrossRef - Extended-Spectrum Beta-Lactamases Producing Escherichia coli in South America: A Systematic Review with a One Health Perspective
Carlos Bastidas-Caldes, Daniel Romero-Alvarez, Victor Valdez-Vélez, Roberto D Morales, Andrés Montalvo-Hernández, Cicero Gomes-Dias, Manuel Calvopiña Infection and Drug Resistance.2022; Volume 15: 5759. CrossRef - The global prevalence and trend of human intestinal carriage of ESBL-producing Escherichia coli in the community
Yihienew M Bezabih, Wilber Sabiiti, Endalkachew Alamneh, Alamneh Bezabih, Gregory M Peterson, Woldesellassie M Bezabhe, Anna Roujeinikova Journal of Antimicrobial Chemotherapy.2021; 76(1): 22. CrossRef - Global prevalence and molecular characterization of extended-spectrum β-lactamase producing-Escherichia coli in dogs and cats – A scoping review and meta-analysis
Marília Salgado-Caxito, Julio A. Benavides, Aiko D. Adell, Antonio Carlos Paes, Andrea I. Moreno-Switt One Health.2021; 12: 100236. CrossRef - Can breastfeeding protect against antimicrobial resistance?
Maya L. Nadimpalli, Claire D. Bourke, Ruairi C. Robertson, Elisabeth Delarocque-Astagneau, Amee R. Manges, Amy J. Pickering BMC Medicine.2020;[Epub] CrossRef - The Epidemiological Pattern and Risk Factor of ESBL (Extended Spectrum Β-Lactamase) Producing Enterobacteriaceae in Gut Bacterial Flora of Dairy Cows and People Surrounding in Rural Area, Indonesia
Agusta Reny Soekoyo, Sulistiawati Sulistiawati, Wahyu Setyorini, Kuntaman Kuntaman Indonesian Journal of Tropical and Infectious Dise.2020; 8(3): 144. CrossRef
|