Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives


Author index

Page Path
HOME > Articles and issues > Author index
María Araque 1 Article
Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing Escherichia coli in Healthy Children from a Rural Andean Village in Venezuela
María Araque, Indira Labrador
Osong Public Health Res Perspect. 2018;9(1):9-15.   Published online December 31, 2017
  • 6,111 View
  • 48 Download
  • 12 Crossref
AbstractAbstract PDF

Antimicrobial resistant extended-spectrum-β-lactamase-producing Enterobacteriaceae (ESBL-PE) have been shown to be present in healthy communities. This study examined healthy children from the rural Andean village of Llano del Hato, Mérida, Venezuela, who have had little or no antibiotic exposure to determine the prevalence of fecal carriage of ESBL-producing Escherichia coli (ESBL-EC).


A total of 78 fecal samples were collected in healthy children aged from 1 to 5 years. ESBL-EC were selected in MacConkey agar plates with cefotaxime and further confirmed by the VITEK 2 system. ESBL were phenotypically detected and presence of bla genes and their variants were confirmed by molecular assays. Determination of phylogenetic groups was performed by PCR amplification. Risk factors associated with fecal carriage of ESBL-EC-positive isolates were analyzed using standard statistical methods.


Of the 78 children studied, 27 (34.6%) carried ESBL-EC. All strains harbored the blaCTX-M-15 allele. Of these, 8 were co-producers of blaTEM-1, blaTEM-5, blaSHV-5 or blaSHV-12. Co-resistance to aminoglycosides and/or fluoroquinolones was observed in 9 strains. 51.9% of ESBL-EC isolates were classified within phylogroup A. A significant, positive correlation was found between age (≥2.5 – ≤5 years), food consumption patterns and ESBL-EC fecal carriage.


This is the first study describing the high prevalence of fecal carriage of ESBL-EC expressing CTX-M-15- among very young, healthy children from a rural Andean village in Venezuela with scarce antibiotic exposure, underlining the importance of this population as a reservoir.


Citations to this article as recorded by  
  • First Report of IMI-2-Producing Enterobacter bugandensis and CTX-M-55-Producing Escherichia coli isolated from Healthy Volunteers in Tunisia
    Rym Ben Sallem, Ameni Arfaoui, Afef Najjari, Isabel Carvalho, Abdelmalek Lekired, Hadda-Imen Ouzari, Karim Ben Slama, Alex Wong, Carmen Torres, Naouel Klibi
    Antibiotics.2023; 12(1): 116.     CrossRef
  • Co-Harboring of Beta-Lactamases and mcr-1 Genes in Escherichia coli and Klebsiella pneumoniae from Healthy Carriers and Backyard Animals in Rural Communities in Ecuador
    Carlos Bastidas-Caldes, Emily Cisneros-Vásquez, Antonella Zambrano, Andrea Mosquera-Maza, William Calero-Cáceres, Joaquín Rey, Yoshimasa Yamamoto, Mayumi Yamamoto, Manuel Calvopiña, Jacobus H. de Waard
    Antibiotics.2023; 12(5): 856.     CrossRef
  • Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae Family of Bacteria from Diarrheal Stool Samples in Northwest Ethiopia
    Minichil Worku, Michael Getie, Feleke Moges, Alem Getaneh Mehari, Jayaraman Tharmalingam
    Interdisciplinary Perspectives on Infectious Disea.2022; 2022: 1.     CrossRef
    Sandra L. Bager, Isaac Kakaala, Egle Kudirkiene, Denis K. Byarugaba, John Elmerdahl Olsen
    Journal of Wildlife Diseases.2022;[Epub]     CrossRef
  • Relevant increase of CTX-M-producing Escherichia coli carriage in school-aged children from rural areas of the Bolivian Chaco in a three-year period
    Selene Rebecca Boncompagni, Maria Micieli, Tiziana Di Maggio, Antonia Mantella, Anna Liz Villagrán, Tatiana Briggesth Miranda, Carmen Revollo, Veronica Poma, Herlan Gamboa, Michele Spinicci, Marianne Strohmeyer, Alessandro Bartoloni, Gian Maria Rossolini,
    International Journal of Infectious Diseases.2022; 121: 126.     CrossRef
  • Comparison of the global prevalence and trend of human intestinal carriage of ESBL-producing Escherichia coli between healthcare and community settings: a systematic review and meta-analysis
    Yihienew M. Bezabih, Alemayehu Bezabih, Michel Dion, Eric Batard, Samson Teka, Abiy Obole, Noah Dessalegn, Alelegn Enyew, Anna Roujeinikova, Endalkachew Alamneh, Corinne Mirkazemi, Gregory M. Peterson, Woldesellassie M. Bezabhe
    JAC-Antimicrobial Resistance.2022;[Epub]     CrossRef
  • Fecal Carriage of Extended-Spectrum β-Lactamases and pAmpC Producing Enterobacterales in an Iranian Community: Prevalence, Risk Factors, Molecular Epidemiology, and Antibiotic Resistance
    Nasrin Habibzadeh, Hadi Peeri Doghaheh, Meysam Manouchehri Far, Hossein Alimohammadi Asl, Sohrab Iranpour, Mohsen Arzanlou
    Microbial Drug Resistance.2022; 28(9): 921.     CrossRef
  • Extended-Spectrum Beta-Lactamases Producing Escherichia coli in South America: A Systematic Review with a One Health Perspective
    Carlos Bastidas-Caldes, Daniel Romero-Alvarez, Victor Valdez-Vélez, Roberto D Morales, Andrés Montalvo-Hernández, Cicero Gomes-Dias, Manuel Calvopiña
    Infection and Drug Resistance.2022; Volume 15: 5759.     CrossRef
  • The global prevalence and trend of human intestinal carriage of ESBL-producing Escherichia coli in the community
    Yihienew M Bezabih, Wilber Sabiiti, Endalkachew Alamneh, Alamneh Bezabih, Gregory M Peterson, Woldesellassie M Bezabhe, Anna Roujeinikova
    Journal of Antimicrobial Chemotherapy.2021; 76(1): 22.     CrossRef
  • Global prevalence and molecular characterization of extended-spectrum β-lactamase producing-Escherichia coli in dogs and cats – A scoping review and meta-analysis
    Marília Salgado-Caxito, Julio A. Benavides, Aiko D. Adell, Antonio Carlos Paes, Andrea I. Moreno-Switt
    One Health.2021; 12: 100236.     CrossRef
  • Can breastfeeding protect against antimicrobial resistance?
    Maya L. Nadimpalli, Claire D. Bourke, Ruairi C. Robertson, Elisabeth Delarocque-Astagneau, Amee R. Manges, Amy J. Pickering
    BMC Medicine.2020;[Epub]     CrossRef
  • The Epidemiological Pattern and Risk Factor of ESBL (Extended Spectrum Β-Lactamase) Producing Enterobacteriaceae in Gut Bacterial Flora of Dairy Cows and People Surrounding in Rural Area, Indonesia
    Agusta Reny Soekoyo, Sulistiawati Sulistiawati, Wahyu Setyorini, Kuntaman Kuntaman
    Indonesian Journal of Tropical and Infectious Dise.2020; 8(3): 144.     CrossRef

PHRP : Osong Public Health and Research Perspectives