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Majid Esmaelizad 1 Article
A Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis
Saeed Alamian, Majid Esmaelizad, Taghi Zahraei, Afshar Etemadi, Mohsen Mohammadi, Davoud Afshar, Soheila Ghaderi
Osong Public Health Res Perspect. 2017;8(1):65-70.   Published online February 28, 2017
DOI: https://doi.org/10.24171/j.phrp.2017.8.1.09
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  • 7 Citations
AbstractAbstract PDF
Objectives

Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis.

Methods

All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available Brucella sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate Brucella abortus from Brucella melitensis. A primer set was designed to flank the unique locus. A total of 136 lymph nodes and blood samples were evaluated and classified by the URS-polymerase chain reaction (PCR) method in 2013–2014.

Results

Biochemical tests and bacteriophage typing as the golden standard indicated that all Brucella spp. isolates were B. melitensis biovar 1 and B. abortus biovar 3. The PCR results were the same as the bacteriological method for detecting Brucella spp. The sensitivity and specificity of the URS-PCR method make it suitable for detecting B. abortus and B. melitensis.

Conclusion

Quick detection of B. abortus and B. melitensis can provide the most effective strategies for control of these bacteria. The advantage of this method over other presented methods is that both B. abortus and B. melitensis are detectable in a single test tube. Furthermore, this method covered 100% of all B. melitensis and B. abortus biotypes. The development of this URS-PCR method is the first step toward the development of a novel kit for the molecular identification of B. abortus and B. melitensis.

Citations

Citations to this article as recorded by  
  • Bovine brucellosis – a comprehensive review
    Sandip Kumar Khurana, Anju Sehrawat, Ruchi Tiwari, Minakshi Prasad, Baldev Gulati, Muhammad Zubair Shabbir, Rajesh Chhabra, Kumaragurubaran Karthik, Shailesh Kumar Patel, Mamta Pathak, Mohd. Iqbal Yatoo, Vivek Kumar Gupta, Kuldeep Dhama, Ranjit Sah, Wanpe
    Veterinary Quarterly.2021; 41(1): 61.     CrossRef
  • Survey of Zoonotic Bacterial Pathogens in Native Foxes in Central Chile: First Record of Brucella canis Exposure
    Nicolás Galarce, Sebastián de la Fuente, Beatriz Escobar, Phillip Dettleff, Pedro Abalos, Juan Carlos Hormazábal, Roberto Flores, Nicole Sallaberry-Pincheira, Víctor Martínez
    Animals.2021; 11(7): 1980.     CrossRef
  • Development and validation of immunoassay for whole cell detection of Brucella abortus and Brucella melitensis
    Richa Hans, Pranjal Kumar Yadav, Pushpendra Kumar Sharma, Mannan Boopathi, Duraipandian Thavaselvam
    Scientific Reports.2020;[Epub]     CrossRef
  • Laboratory Diagnostic Procedures for Human Brucellosis: An Overview of Existing Approaches
    Afshar Etemadi, Rezvan Moniri, Heinrich Neubauer, Yasaman Dasteh Goli, Saeed Alamian
    Jundishapur Journal of Microbiology.2019;[Epub]     CrossRef
  • Comparison of PCR-RFLP and PFGE for determining the clonality of Brucella isolates from human and livestock specimens
    Nasrin Bahmani, Reza Mirnejad, Mohammad Reza Arabestani, Parviz Mohajerie, Seyed Hamid Hashemi, Manoochehr Karami, Mohammad Yousef Alikhani
    Saudi Journal of Biological Sciences.2019; 26(2): 256.     CrossRef
  • Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles
    Arash Shams, Bahareh Rahimian Zarif, Mojtaba Salouti, Reza Shapouri, Sako Mirzaii
    Artificial Cells, Nanomedicine, and Biotechnology.2019; 47(1): 2562.     CrossRef
  • Identification of Brucella genus and eight Brucella species by Luminex bead-based suspension array
    Tina S. Lusk Pfefer, Ruth Timme, Julie A. Kase
    Food Microbiology.2018; 70: 113.     CrossRef

PHRP : Osong Public Health and Research Perspectives