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Mahyarudin Mahyarudin 1 Article
Immunoinformatics approach for design novel multi-epitope prophylactic and therapeutic vaccine based on capsid proteins L1 and L2 and oncoproteins E6 and E7 of human papillomavirus 16 and human papillomavirus 18 against cervical cancer
Nicholas Ryan, Sari Eka Pratiwi, Mardhia Mardhia, Ysrafil Ysrafil, Delima Fajar Liana, Mahyarudin Mahyarudin
Osong Public Health Res Perspect. 2024;15(4):307-328.   Published online July 23, 2024
DOI: https://doi.org/10.24171/j.phrp.2024.0013
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  • 1 Web of Science
  • 1 Crossref
Graphical AbstractGraphical Abstract AbstractAbstract PDF
Objectives
This study aimed to identify the optimal protein construction for designing a multi-epitope vaccine with both prophylactic and therapeutic effects against cervical cancer, utilizing an immunoinformatics approach. The construction process involved using capsid epitopes L1 and L2, as well as oncoproteins E5, E6, and E7 from human papillomavirus (HPV) types 16 and 18.
Methods
An experimental in silico analysis with an immunoinformatics approach was used to develop 2 multi-epitope vaccine constructs (A and B). Further analysis was then conducted to compare the constructs and select the one with the highest potential against cervical cancer.
Results
This study produced 2 antigenic, non-allergenic, and nontoxic multi-epitope vaccine constructs (A and B), which exhibited the ideal physicochemical properties for a vaccine. Further analysis revealed that construct B effectively induced both cellular and humoral immune responses.
Conclusion
The multi-epitope vaccine construct B for HPV 16 and 18, designed for both prophylactic and therapeutic purposes, met the development criteria for a cervical cancer vaccine. However, these findings need to be validated through in vitro and in vivo experiments.

Citations

Citations to this article as recorded by  
  • Modulation of epithelial homeostasis by HPV using Notch and Wnt
    June See Chong, John Doorbar
    Tumour Virus Research.2024; 18: 200297.     CrossRef

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