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Mahmood Mahmoudi 1 Article
Profiling of Virulence-associated Factors in Shigella Species Isolated from Acute Pediatric Diarrheal Samples in Tehran, Iran
Sajad Yaghoubi, Reza Ranjbar, Mohammad Mehdi Soltan Dallal, Somayeh Yasliani Fard, Mohammad Hasan Shirazi, Mahmood Mahmoudi
Osong Public Health Res Perspect. 2017;8(3):220-226.   Published online June 30, 2017
DOI: https://doi.org/10.24171/j.phrp.2017.8.3.09
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  • 14 Citations
AbstractAbstract PDF
Objectives

The genus Shigella comprises the most infectious and diarrheagenic bacteria causing severe diseases, mostly in children under five years of age. This study aimed to detect nine virulence genes (ipaBCD, VirA, sen, set1A, set1B, ial, ipaH, stx, and sat) in Shigella species (spp.) using multiplex polymerase chain reaction (MPCR) and to determine the relation of Shigella spp. from pediatric diarrheal samples with hospitalization and bloody diarrhea in Tehran, Iran.

Methods

Shigella spp. were isolated and identified using standard microbiological and serological methods. The virulence genes were detected using MPCR.

Results

Seventy-five Shigella spp. (40 S. sonnei, 33 S. flexneri, 1 S. dysenteriae, and 1 S. boydii) were isolated in this study. The prevalence of ial, sen, sat, set1A, and set1B was 74.7%, 45.4%, 28%, 24%, and 24%, respectively. All S. flexneri isolates, while no S. sonnei, S. dysenteriae, or S. boydii isolates, contained sat, set1A, and set1B. All isolates were positive for ipaH, ipaBCD, and virA, while one (1.4%) of the isolates contained stx. The highest prevalence of virulence determinants was found in S. flexneri serotype IIa. Nineteen (57.6%) of 33 S. flexneri isolates were positive for ipaBCD, ipaH, virA, ial, and sat. The sen determinants were found to be statistically significantly associated with hospitalization and bloody diarrhea (p = 0.001).

Conclusion

This study revealed a high prevalence of enterotoxin genes in S. flexneri, especially in serotype 2a, and has presented relations between a few clinical features of shigellosis and numerous virulence determinants of clinical isolates of Shigella spp.

Citations

Citations to this article as recorded by  
  • Distribution of genes encoding virulence factors of Shigella strains isolated from children with diarrhea in southwest Iran
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    BMC Microbiology.2021;[Epub]     CrossRef
  • Development of quinolone resistance and prevalence of different virulence genes among Shigella flexneri and Shigella dysenteriae in environmental water samples
    B. Roy, S.K. Tousif Ahamed, B. Bandyopadhyay, N. Giri
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  • High Rates of Antimicrobial Resistance and Virulence Gene Distribution Among Shigella spp. Isolated from Pediatric Patients in Tehran, Iran


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  • Molecular characterization of Shigella species isolated from diarrheal patients in Tehran, Iran: phylogenetic typing and its association with virulence gene profiles and a novel description of Shigella invasion associated locus
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  • Case report on a swift shift in uropathogens from Shigella flexneri to Escherichia coli: a thin line between bacterial persistence and reinfection
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    Annals of Clinical Microbiology and Antimicrobials.2020;[Epub]     CrossRef
  • Virulence-related genes are associated with clinical and nutritional outcomes of Shigella/Enteroinvasive Escherichia coli pathotype infection in children from Brazilian semiarid region: A community case-control study
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  • Genotyping and diversity of virulence genes among Shigella sonnei isolated from children with diarrhoea
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  • Virulence gene profiles of Shigella species isolated from stool specimens in India: its association with clinical manifestation and antimicrobial resistance
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    Pathogens and Global Health.2019; 113(4): 173.     CrossRef
  • Prevalence of enterotoxin-encoding genes among diverse Shigella strains isolated from patients with diarrhea, southwest Iran
    Mojtaba Moosavian, Sakineh Seyed-Mohammadi, Ahmad Farajzadeh Sheikh, Saeed Khoshnood, Aram Asarehzadegan Dezfuli, Morteza Saki, Gholamreza Ghaderian, Fatemeh Shahi, Mahtab Abdi, Fariba Abbasi
    Acta Microbiologica et Immunologica Hungarica.2018; 66(1): 91.     CrossRef
  • Frequency of Mutations in Quinolone Resistance-Determining Regions and Plasmid-Mediated Quinolone Resistance in Shigella Isolates Recovered from Pediatric Patients in Tehran, Iran: An Overlooked Problem
    Sajad Yaghoubi, Reza Ranjbar, Mohammad Mehdi Soltan Dallal, Mohammad Hasan Shirazi, Mohammad Kazem Sharifi-Yazdi
    Microbial Drug Resistance.2018; 24(6): 699.     CrossRef

PHRP : Osong Public Health and Research Perspectives