<b>Objectives</b><br/> The aim of this study was to investigate the distribution of Shiga toxin (Stx) gene-positive stool samples from dairy farmer and slaughterhouse workers in Gyeonggi-Do province.<br/><b>Methods</b><br/> A total of 621 samples from healthy farmers and 198 samples from slaughterhouse workers were screened by polymerase chain reaction (PCR) for Shiga toxigenic <i>Escherichia coli</i> (STEC) infection on stool samples.<br/><b>Results</b><br/> The PCR product of Stx-encoding genes was detected in 21 (3.4%) of 621 farmers and 15 (7.6%) of 198 slaughterhouse workers’ stool samples. Distribution of the <i>Stx</i> PCR positive workers by age increment revealed an increase in STEC infection with age increment in both workers. Distribution of the <i>Stx</i> PCR positive workers by working years revealed an increase in STEC infection with working years in farmers.<br/><b>Conclusion</b><br/> These results of the study show that slaughterhouse workers are at higher risk of STEC infection than farmers. In addition, slaughterhouse workers have a more potential source of food contamination of STEC and transmission.