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Kyeongcheol Shin 2 Articles
Development of a Specific and Rapid Diagnostic Method for Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay
Mi Jung Ji, Byung Ki Cho, Young Shik Cho, Young Jin Choi, Donghyok Kwon, Kyeongcheol Shin, Joo-Yeon Lee, Chun Kang, Byoung Su Yoon
Osong Public Health Res Perspect. 2013;4(6):342-346.   Published online December 31, 2013
DOI: https://doi.org/10.1016/j.phrp.2013.10.006
  • 2,086 View
  • 13 Download
  • 2 Citations
AbstractAbstract PDF
Objectives
The aim of this study was to develop an immunochromatographic assay (ICA) for the detection of influenza A (H1N1) pdm09 virus infection. Materials and methods Several monoclonal antibodies against influenza A (H1N1) pdm09 virus were generated and an ICA (pdm09-ICA) was developed for the rapid and specific detection of influenza A (H1N1) pdm09 virus infection. The specificity and sensitivity of the developed assay were compared with that of hemagglutination assay and real-time reverse-transcription polymerase chain reaction (rRT-PCR).
Results
The detection limit was estimated to be 1/2 (8) hemagglutinating unit; the sensitivity and specificity rates of pdm09-ICA were 75.86% (110/145) and 100% (43/43), respectively, compared with rRT-PCR. The cross-reactivity for 20 influenza viruses, including seasonal H1N1 viruses, was found to be negative except for the H1N1 virus (A/Swine/Korea/GC0503/2005).
Conclusion
These results indicate that the proposed method can be easily used for rapid and specific detection of the pdm09 infection. The assay developed in this study would be a useful tool for distinguishing the pdm09 infection from seasonal influenza A and B infections.

Citations

Citations to this article as recorded by  
  • Sensitive detection of influenza a virus based on a CdSe/CdS/ZnS quantum dot-linked rapid fluorescent immunochromatographic test
    Anh Viet Thi Nguyen, Tung Duy Dao, Tien Thi Thuy Trinh, Du-Young Choi, Seung-Taek Yu, Hyun Park, Seon-Ju Yeo
    Biosensors and Bioelectronics.2020; 155: 112090.     CrossRef
  • Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay
    Viroj Wiwanitkit
    Osong Public Health and Research Perspectives.2014; 5(2): 115.     CrossRef
Pathogenesis and Chronologic Localization of the Human Influenza A (H1N1) Virus in Cotton Rats
Donghyok Kwon, Kyeongcheol Shin, Jin-Young Shin, Joo-Yeon Lee, Yooncheol Ha, Nam-Joo Lee, Hee-Bok Oh, Chanhee Chae, Chun Kang
Osong Public Health Res Perspect. 2011;2(1):15-22.   Published online June 30, 2011
DOI: https://doi.org/10.1016/j.phrp.2011.04.005
  • 2,096 View
  • 22 Download
  • 7 Citations
AbstractAbstract PDF
Objectives
We aimed to evaluate the pathogenesis and chronologic localization of human influenza A (H1N1) virus in experimentally infected cotton rats.
Methods
The animals were intranasally inoculated with 107 plaque-forming units of A/Solomon Islands/3/2006 (H1N1) influenza virus and evaluated for pathogenicity for a period of 28 days. Virus replication kinetics and pathological properties were assessed chronologically. Acute antiviral responses were evaluated by mean of real-time polymerase chain reaction.
Results
Cotton rats infected with A/Solomon Islands/3/2006 virus lost weight until 6 days post-inoculation (DPI) and showed decreased activity until 3 DPI. At necropsy, focal areas of redness and consolidation of lungs were evident at 1, 2, and 3 DPI. Lung histopathology showed moderate to severe interstitial pneumonia, alveolitis and bronchiolitis. Influenza A specific viral protein was detected in bronchiolar epithelial cells, alveolar septa and pneumocytes. Influenza viruses were recovered from the lungs during the early period of infection and the titer peaked at 1 DPI. Viral proteins were detected from 4 hours to 6 hours DPI. These trends correlate with the up-regulation of mRNA expression of the IFN-α, Mx1, and Mx2 genes that play critical roles in the anti-influenza response at the early stage of infection.
Conclusion
Our results provide evidence that supports the use of cotton rats for the study of influenza virus pathogenesis and the immune response.

Citations

Citations to this article as recorded by  
  • Characterization of humoral immune responses and degree of protection induced by influenza vaccine in cotton rats: Effects of low vaccine dose and single vs booster vaccination
    Yoshita Bhide, Wei Dong, Tjarko Meijerhof, Jacqueline Vries‐Idema, Hubert G. Niesters, Anke Huckriede
    Immunity, Inflammation and Disease.2020; 8(3): 279.     CrossRef
  • Doing Mathematics with Aftermath of Pandemic Influenza 2009
    Hae-Wol Cho, Chaeshin Chu
    Osong Public Health and Research Perspectives.2015; 6(1): 1.     CrossRef
  • Assessment of Intensive Vaccination and Antiviral Treatment in 2009 Influenza Pandemic in Korea
    Chaeshin Chu, Sunmi Lee
    Osong Public Health and Research Perspectives.2015; 6(1): 47.     CrossRef
  • Mammalian pathogenesis of oseltamivir-resistant pandemic (H1N1) 2009 influenza virus isolated in South Korea
    Donghyok Kwon, Kyeongcheol Shin, Su-Jin Kim, Joo-Yeon Lee, Chun Kang
    Virus Research.2014; 185: 41.     CrossRef
  • Was the Mass Vaccination Effective During the Influenza Pandemic 2009–2010 in Korea?
    Hae-Wol Cho, Chaeshin Chu
    Osong Public Health and Research Perspectives.2013; 4(4): 177.     CrossRef
  • How to Manage a Public Health Crisis and Bioterrorism in Korea
    Hae-Wol Cho, Chaeshin Chu
    Osong Public Health and Research Perspectives.2013; 4(5): 223.     CrossRef
  • The Road Less Traveled
    Chaeshin Chu
    Osong Public Health and Research Perspectives.2011; 2(1): 1.     CrossRef

PHRP : Osong Public Health and Research Perspectives