- Characterization of Clostridium difficile Strains Isolated from Patients with C. difficile-associated Disease in Korea
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Seung-Hak Cho, Jung-Whan Chon, Kun-Ho Seo, Young Kwon Kim, Jung-Beom Kim, Young-Seok Bak, Woon-Won Jung, Cheorl-Ho Kim, Jong Tae Choi
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Osong Public Health Res Perspect. 2017;8(5):325-331. Published online October 31, 2017
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DOI: https://doi.org/10.24171/j.phrp.2017.8.5.06
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 Abstract
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- Objectives
Studies on Clostridium difficile are rare in Korea. We investigated the epidemiological characteristics of C. difficile isolates from patients with C. difficile-associated disease (CDAD) in Korea. MethodsMultiplex polymerase chain reaction was performed to detect the presence of tcdA and tcdB toxin genes. Antimicrobial susceptibility test was carried out by the disk-dilution method. C. difficile strains were subtyped by automated repetitive-element palindromic PCR (rep-PCR). ResultsAmong patients with CDAD, 73 (25.8%), 32 (11.3%), 32 (11.3%), and 26 (9.2%) suffered from pneumonia, cancer or neoplasm, diabetes, and colitis, respectively. Of all stool samples, 43 samples (15.2%) were positive for C. difficile strains. We observed two expression patterns of toxin genes: tcdA+/tcdB+ (86% isolates) and tcdA−/tcdB+ (14% isolates), with all isolates expressing tcdB. Furthermore, some isolates were resistant to clindamycin (65%), ampicillin (56%), and cefazolin (40%), but all were susceptible to vancomycin and metronidazole. The tested samples were classified into diverse clusters using automated rep-PCR. ConclusionOur findings revealed the characteristics and antibiotic resistance of C. difficile isolates from patients in Korea. The epidemiological data may provide valuable insight into development of treatment strategies for C. difficile infections in Korea.
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Citations
Citations to this article as recorded by  - Metronidazole therapy as initial treatment of Clostridium difficile infection in patients with chronic kidney disease in Korea
Jaeuk Shin, Yu Mi Wi, Yu-Ji Lee
Epidemiology and Infection.2019;[Epub] CrossRef
- Corrigendum to “A Comparison of Subtyping Methods for Differentiating Salmonella enteric Serovar Enteritidis Isolates Obtained from Food and Human Sources” [Volume 4, Issue 1, February 2013, Pages 27–33]
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Ji-Yeon Hyeon, Jung-Whan Chon, Jun-Ho Park, Moo-Sang Kim, Young-Hee Oh, In-Soo Choi, Kun-Ho Seo
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Osong Public Health Res Perspect. 2013;4(3):176-176. Published online June 30, 2013
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DOI: https://doi.org/10.1016/j.phrp.2013.05.002
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- A Comparison of Subtyping Methods for Differentiating Salmonella enterica Serovar Enteritidis Isolates Obtained from Food and Human Sources
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Ji-Yeon Hyeon, Jung-Whan Chon, Jun-Ho Park, Moo-Sang Kim, Young-Hee Oh, In-Soo Choi, Kun-Ho Seo
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Osong Public Health Res Perspect. 2013;4(1):27-33. Published online February 28, 2013
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DOI: https://doi.org/10.1016/j.phrp.2012.12.005
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2,479
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Abstract
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- Purpose:
To evaluate the abilities of these subtyping methods, we distinguished Salmonella Enteritidis (S. Enteritidis) isolated from food products and human clinical samples between 2009 and 2010 in Seoul using five subtyping methods.
Methods
We determined the subtypes of 20 S. Enteritidis isolates from food and human sources using phage typing, antimicrobial susceptibility, pulsed-field gel electrophoresis (PFGE), repetitive sequence-based PCR (rep-PCR), and multi-locus sequence typing (MLST).
Results
A total of 20 tested isolates were differentiated into six antimicrobial susceptibility patterns, three different phage types, four different PFGE profiles, seven rep-PCR patterns, and one MLST type. Food isolates were considerably more susceptible to antibiotics than human isolates. We were best able to discriminate among S. Enteritidis isolates using rep-PCR, and obtained the highest Simpson’s diversity index of 0.82, whereas other methods produced indices that were less than 0.71. PFGE pattern appeared to be more related to antimicrobial resistance and phage types of S. Enteritidis isolates than rep-PCR. MLST revealed identical alleles in all isolates at all seven loci examined, indicating no resolution.
Conclusion
The results of this study suggest that rep-PCR provided the best discriminatory power for phenotypically similar S. Enteritidis isolates of food and human origins, whereas the discriminatory ability of MLST may be problematic because of the high sequence conservation of the targeted genes.
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Citations
Citations to this article as recorded by - Phenotypic and Genotypic Characterization of Salmonella Isolates Recovered from Foods Linked to Human Salmonellosis Outbreaks in Minas Gerais State, Brazil
Leandro L. Faula, Monica M. O.P. Cerqueira, Jovita E. G.C. Madeira, Marcelo R. Souza, Fernando N. Souza, Gláucia C.S. Amancio
Journal of Food Protection.2022; 85(1): 142. CrossRef - Comparison of conventional molecular and whole-genome sequencing methods for subtyping Salmonella enterica serovar Enteritidis strains from Tunisia
Boutheina Ksibi, Sonia Ktari, Houcemeddine Othman, Kais Ghedira, Sonda Maalej, Basma Mnif, Mohamed salah Abbassi, Laetitia Fabre, Faouzia Rhimi, Simon Le Hello, Adnene Hammami
European Journal of Clinical Microbiology & Infect.2021; 40(3): 597. CrossRef - Molecular characterisation of antimicrobial resistance determinants and class 1 integrons of Salmonella enterica subsp. enterica serotype Enteritidis strains from retail food in China
Yu Li, Xiaojuan Yang, Jumei Zhang, Shiyuan Yang, Shuhong Zhang, Moutong Chen, Liang Xue, Yu Ding, Haiyan Zeng, Qihui Gu, Youxiong Zhang, Xianhu Wei, Juan Wang, Qingping Wu
Food Control.2021; 128: 108191. CrossRef - Molecular typing of cephalosporin resistant serovars of Salmonella enterica from poultry and farm animals
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Diana Ayala, Kendra Nightingale, Claudia Narvaez-Bravo, Mindy M. Brashears
Journal of Food Protection.2017; 80(12): 1964. CrossRef - Repetitive sequence-based PCR fingerprinting and the relationship of antimicrobial-resistance characteristics and corresponding genes among Salmonella strains from pig production
Teerarat Prasertsee, Nattakarn Khantaprab, Panuwat Yamsakul, Pannita Santiyanont, Nipa Chokesajjawatee, Prapas Patchanee
Asian Pacific Journal of Tropical Disease.2016; 6(5): 390. CrossRef - Prevalence, Seasonal Occurrence, and Antimicrobial Resistance of Salmonella spp. Isolates Recovered from Chicken Carcasses Sampled at Major Poultry Processing Plants of South Korea
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Foodborne Pathogens and Disease.2015; 12(3): 190. CrossRef - Comparison of four molecular methods to typeSalmonellaEnteritidis strains
Fábio Campioni, André Pitondo-Silva, Alzira M.M. Bergamini, Juliana P. Falcão
APMIS.2015; 123(5): 422. CrossRef - Modified Method of Multilocus Sequence Typing (MLST) for Serotyping inSalmonellaSpecies
Young-Sun Yun, Su-Jin Chae, Hye-Young Na, Gyung Tae Chung, Cheon-Kwon Yoo, Deog-Yong Lee
Journal of Bacteriology and Virology.2015; 45(4): 314. CrossRef - Molecular Characterization of Salmonella Paratyphi B dT + and Salmonella Heidelberg from Poultry and Retail Chicken Meat in Colombia by Pulsed-Field Gel Electrophoresis
Pilar Donado-Godoy, Barbara A. Byrne, Michael Hume, Maribel León, Enrique Pérez-Gutiérrez, Martha J. Vives Flores, Viviana Clavijo, Ángela Holguin, Juan J. Romero-Zuňiga, Ricardo Castellanos, Mcallister Tafur, Woutrina A. Smith
Journal of Food Protection.2015; 78(4): 802. CrossRef - Prevalence Analysis and Molecular Characterization ofSalmonellaat Different Processing Steps in Broiler Slaughter Plants in South Korea
Hyun-Jung Park, Jung-Whan Chon, Jong-Soo Lim, Kun-Ho Seo, Young-Jo Kim, Eun-Jeong Heo, Sung-Hwan Wee, Kidon Sung, Jin-San Moon
Journal of Food Science.2015; 80(12): M2822. CrossRef - Characterization of Escherichia coli–Producing Extended-Spectrum β-Lactamase (ESBL) Isolated from Chicken Slaughterhouses in South Korea
Jong-Soo Lim, Da-Som Choi, Young-Jo Kim, Jung-Whan Chon, Hong-Seok Kim, Hyun-Jung Park, Jin-San Moon, Sung-Hwan Wee, Kun-Ho Seo
Foodborne Pathogens and Disease.2015; 12(9): 741. CrossRef - Comparative Analysis of Subtyping Methods against a Whole-Genome-Sequencing Standard for Salmonella enterica Serotype Enteritidis
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Poultry Science.2013; 92(10): 2789. CrossRef
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