- Multiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus
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Jie Yeun Park, Semi Jeon, Jun Young Kim, Misun Park, Seonghan Kim
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Osong Public Health Res Perspect. 2013;4(3):133-139. Published online June 30, 2013
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DOI: https://doi.org/10.1016/j.phrp.2013.04.004
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Abstract
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- Objectives
A multiplex real-time polymerase chain reaction (RT-PCR) method was developed for the identification of three Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. Methods
Specific primers and probes targeting the hlyA, tlh, and vvhA genes were selected and used for multiplex real-time PCR to confirm the identification of V. cholerae, V. parahaemolyticus, and V. vulnificus, respectively. This method was applied to screen Vibrio species from environmental samples and combining it with a culture-based method, its effectiveness was evaluated in comparison with culture-based methods alone. Results
Specific PCR fragments were obtained from isolates belonging to the target species, indicating a high specificity of this multiplex real-time PCR. No cross-reactivity with the assay was observed between the tested bacteria. The sensitivity of the multiplex real-time PCR was found to have a lower limit of 104 colony-forming units/reaction for all three Vibrio species. The combination strategy raised the isolation ratio of all three Vibrio species 1.26- to 2.75-fold. Conclusion
This assay provides a rapid, sensitive, and specific technique to detect these three Vibrio species in the environment.
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Citations
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- Genotypic Characterization of Vibrio vulnificus Clinical Isolates in Korea
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Hye Sook Jeong, Jun Young Kim, Se Mi Jeon, Mi Sun Park, Seong Han Kim
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Osong Public Health Res Perspect. 2011;2(1):8-14. Published online June 30, 2011
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DOI: https://doi.org/10.1016/j.phrp.2011.04.008
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3,224
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20
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Abstract
PDF
- Objectives
Vibrio vunificus is known to cause septicemia and severe wound infections in patients with chronic liver diseases or an immuno-compromised condition. We carried out the molecular characterization of V. vulnificus isolates from human Vibrio septicemia cases based on pulsed-field gel electrophoresis (PFGE) using NotI and SfiI. Methods and Results
PFGE was used to characterize a total of 78 strains from clinical cases after NotI or SfiI digestion. The geographical distribution of PFGE patterns for the strains from the southern part of Korea, a high-risk region for Vibrio septicemia, indicated that the isolates from southeastern Korea showed a comparatively higher degree of homology than those from southwestern Korea. Conclusions
We report the genetic distribution of V. vulnficus isolated from Vibrio septicemia cases during 2000–2004 in Korea. This method has potential use as a subspecies-typing tool for V. vulnificus strains isolated from distant geographic regions.
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Citations
Citations to this article as recorded by
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Caroline D’Souza, Ballamoole Krishna Kumar, Sachidananda Kapinakadu, Ranjith Shetty, Indrani Karunasagar, Iddya Karunasagar International Journal of Infectious Diseases.2018; 68: 74. CrossRef - Genotypic Diversity and Population Structure of Vibrio vulnificus Strains Isolated in Taiwan and Korea as Determined by Multilocus Sequence Typing
Hye-Jin Kim, Jae-Chang Cho, Paul J Planet PLOS ONE.2015; 10(11): e0142657. CrossRef - The Road Less Traveled
Chaeshin Chu Osong Public Health and Research Perspectives.2011; 2(1): 1. CrossRef
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