- Original Article
- Generation and Characterization of Recombinant Influenza A(H1N1) Viruses Resistant to Neuraminidase Inhibitors
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WooYoung Choi, Jin-Young Shin, Hwan-Eui Jeong, Mi-Jin Jeong, Su-Jin Kim, Joo-Yeon Lee, Chun Kang
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Osong Public Health Res Perspect. 2013;4(6):323-328. Published online December 31, 2013
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Objectives
To examine the effect of neuraminidase (NA) mutations on the NA inhibitor (NAI) resistance phenotype, the recombinant influenza A/Chungbuk/4448/2008(H1N1) virus isolated in South Korea during the 2008–2009 season was generated by reverse genetics.
Methods
Site-directed mutagenesis was introduced on the NA gene of A/Chungbuk/4448/2008(H1N1) virus, and a total of 23 single, double, and triple mutants were generated. Resistance phenotype of these recombinant... |
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- Development of a Specific and Rapid Diagnostic Method for Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay
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Mi Jung Ji, Byung Ki Cho, Young Shik Cho, Young Jin Choi, Donghyok Kwon, Kyeongcheol Shin, Joo-Yeon Lee, Chun Kang, Byoung Su Yoon
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Osong Public Health Res Perspect. 2013;4(6):342-346. Published online December 31, 2013
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Objectives
The aim of this study was to develop an immunochromatographic assay (ICA) for the detection of influenza A (H1N1) pdm09 virus infection.
Materials and methods
Several monoclonal antibodies against influenza A (H1N1) pdm09 virus were generated and an ICA (pdm09-ICA) was developed for the rapid and specific detection of influenza A (H1N1) pdm09 virus infection. The specificity and sensitivity of the developed... |
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- Articles
- The Emergence of Oseltamivir-Resistant Seasonal Influenza A (H1N1) Virus in Korea During the 2008-2009 Season
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Woo-Young Choi, Inseok Yang, Sujin Kim, Namjoo Lee, Meehwa Kwon, Joo-Yeon Lee, Chun Kang
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Osong Public Health Res Perspect. 2011;2(3):178-185. Published online December 31, 2011
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Objectives
To monitor antiviral drug resistance among seasonal influenza viruses isolated in Korea during the 2008-2009 influenza season, we examined influenza isolates collected through Korea Influenza Surveillance Scheme for antiviral drug susceptibility.
Methods
For genetic analysis of antiviral drug resistance, the matrix (M2) and neuraminidase (NA) genes of each isolate were amplified by reverse transcription-polymerase chain reaction and followed by nucleotide sequencing. For... |
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- Original Article
- Pathogenesis and Chronologic Localization of the Human Influenza A (H1N1) Virus in Cotton Rats
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Donghyok Kwon, Kyeongcheol Shin, Jin-Young Shin, Joo-Yeon Lee, Yooncheol Ha, Nam-Joo Lee, Hee-Bok Oh, Chanhee Chae, Chun Kang
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Osong Public Health Res Perspect. 2011;2(1):15-22. Published online June 30, 2011
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Objectives
We aimed to evaluate the pathogenesis and chronologic localization of human influenza A (H1N1) virus in experimentally infected cotton rats.
Methods
The animals were intranasally inoculated with 107 plaque-forming units of A/Solomon Islands/3/2006 (H1N1) influenza virus and evaluated for pathogenicity for a period of 28 days. Virus replication kinetics and pathological properties were assessed chronologically. Acute antiviral responses were evaluated by mean of... |
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