- Generation and Characterization of Recombinant Influenza A(H1N1) Viruses Resistant to Neuraminidase Inhibitors
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WooYoung Choi, Jin-Young Shin, Hwan-Eui Jeong, Mi-Jin Jeong, Su-Jin Kim, Joo-Yeon Lee, Chun Kang
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Osong Public Health Res Perspect. 2013;4(6):323-328. Published online December 31, 2013
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DOI: https://doi.org/10.1016/j.phrp.2013.10.005
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3,474
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Abstract
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- Objectives
To examine the effect of neuraminidase (NA) mutations on the NA inhibitor (NAI) resistance phenotype, the recombinant influenza A/Chungbuk/4448/2008(H1N1) virus isolated in South Korea during the 2008–2009 season was generated by reverse genetics. Methods
Site-directed mutagenesis was introduced on the NA gene of A/Chungbuk/4448/2008(H1N1) virus, and a total of 23 single, double, and triple mutants were generated. Resistance phenotype of these recombinant viruses was determined by NA-inhibition (NAI) assays based on a fluorometric method using two NAIs (oseltamivir and zanamivir). Results
NA-inhibition assays showed that all the single and double mutants containing the Y275 except the single Y275-E119V mutant conferred important levels of resistance to oseltamivir, whereas all the single, double, and triple mutants containing the E119V mutation were associated with the resistance to zanamivir. Conclusion
Considering the effect of mutations in NA gene on the resistance to NAIs, it is important to monitor the possible emergence and dissemination of multidrug-resistant variants in the human population due to amino acid changes at NA gene as well as to develop novel NAIs.
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- Phylogenetic analysis and docking study of neuraminidase gene of influenza A/H1N1 viruses circulating in Iran from 2010 to 2019
Sina Moeini, Atefeh Mohebbi, Behrokh Farahmand, Parvaneh Mehrbod, Fatemeh Fotouhi Virus Research.2023; 334: 199182. CrossRef - SEQUENCE‐FREE PHYLOGENETICS WITH MASS SPECTROMETRY
Kevin M. Downard Mass Spectrometry Reviews.2022; 41(1): 3. CrossRef - Next-Generation Sequencing: An Eye-Opener for the Surveillance of Antiviral Resistance in Influenza
Laura A.E. Van Poelvoorde, Xavier Saelens, Isabelle Thomas, Nancy H. Roosens Trends in Biotechnology.2020; 38(4): 360. CrossRef - Mutation-induced spatial differences in neuraminidase structure and sensitivity to neuraminidase inhibitors
Zhi-wei Yang, Dong-xiao Hao, Yi-zhuo Che, Jia-hui Yang, Lei Zhang, Sheng-li Zhang Chinese Physics B.2018; 27(1): 018704. CrossRef - Authorised medicinal product Aspecton® Oral Drops containing thyme extract KMTv24497 shows antiviral activity against viruses which cause respiratory infections
Eva Lenz, Christin Müller, Ahmed Mostafa, Julia Dzieciolowski, Pumaree Kanrai, Sharmistha Dam, Ute Cwientzek, Lars-Norbert Prenner, Stephan Pleschka Journal of Herbal Medicine.2018; 13: 26. CrossRef - Ultrasensitive Fluorogenic Reagents for Neuraminidase Titration
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- Development of a Specific and Rapid Diagnostic Method for Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay
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Mi Jung Ji, Byung Ki Cho, Young Shik Cho, Young Jin Choi, Donghyok Kwon, Kyeongcheol Shin, Joo-Yeon Lee, Chun Kang, Byoung Su Yoon
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Osong Public Health Res Perspect. 2013;4(6):342-346. Published online December 31, 2013
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DOI: https://doi.org/10.1016/j.phrp.2013.10.006
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3,540
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Abstract
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- Objectives
The aim of this study was to develop an immunochromatographic assay (ICA) for the detection of influenza A (H1N1) pdm09 virus infection.
Materials and methods
Several monoclonal antibodies against influenza A (H1N1) pdm09 virus were generated and an ICA (pdm09-ICA) was developed for the rapid and specific detection of influenza A (H1N1) pdm09 virus infection. The specificity and sensitivity of the developed assay were compared with that of hemagglutination assay and real-time reverse-transcription polymerase chain reaction (rRT-PCR). Results
The detection limit was estimated to be 1/2 (8) hemagglutinating unit; the sensitivity and specificity rates of pdm09-ICA were 75.86% (110/145) and 100% (43/43), respectively, compared with rRT-PCR. The cross-reactivity for 20 influenza viruses, including seasonal H1N1 viruses, was found to be negative except for the H1N1 virus (A/Swine/Korea/GC0503/2005). Conclusion
These results indicate that the proposed method can be easily used for rapid and specific detection of the pdm09 infection. The assay developed in this study would be a useful tool for distinguishing the pdm09 infection from seasonal influenza A and B infections.
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Citations
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- Sensitive detection of influenza a virus based on a CdSe/CdS/ZnS quantum dot-linked rapid fluorescent immunochromatographic test
Anh Viet Thi Nguyen, Tung Duy Dao, Tien Thi Thuy Trinh, Du-Young Choi, Seung-Taek Yu, Hyun Park, Seon-Ju Yeo Biosensors and Bioelectronics.2020; 155: 112090. CrossRef - Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay
Viroj Wiwanitkit Osong Public Health and Research Perspectives.2014; 5(2): 115. CrossRef
- The Emergence of Oseltamivir-Resistant Seasonal Influenza A (H1N1) Virus in Korea During the 2008-2009 Season
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Woo-Young Choi, Inseok Yang, Sujin Kim, Namjoo Lee, Meehwa Kwon, Joo-Yeon Lee, Chun Kang
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Osong Public Health Res Perspect. 2011;2(3):178-185. Published online December 31, 2011
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DOI: https://doi.org/10.1016/j.phrp.2011.11.042
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3,402
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Abstract
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- Objectives
To monitor antiviral drug resistance among seasonal influenza viruses isolated in Korea during the 2008-2009 influenza season, we examined influenza isolates collected through Korea Influenza Surveillance Scheme for antiviral drug susceptibility. Methods
For genetic analysis of antiviral drug resistance, the matrix (M2) and neuraminidase (NA) genes of each isolate were amplified by reverse transcription-polymerase chain reaction and followed by nucleotide sequencing. For phylogenetic analyses, the sequences of hemagglutinin (HA) and NA genes of each isolate were aligned using multiple alignment program. For phenotypic analysis of antiviral drug resistance, drug susceptibilities against M2 inhibitor (amantadine) and NA inhibitors (oseltavimir and zanamivir) were determined by virus yield reduction assay and fluorometric NA inhibition assay, respectively. Results
In Korea, the resistant influenza viruses against oseltamivir were first detected in sealsonal influenza A(H1N1) viruses on Week 48 of 2008. Since then, the number of oseltamivir-resistant A(H1N1) viruses was continuously increased and had reached the highest peak on Week 52 of 2008. 533 (99.8%) of 534 A(H1N1) viruses were resistant to oseltamivir and all of them harbored the H275Y mutation in the NA gene during the 2008-2009 season. The oseltamivir resistance identified by sequencing was confirmed by NA inhibition assay. Genetic analysis based on HA gene of the resistant A(H1N1) viruses revealed that the viruses were identified as A/Brisbane/10/2007-like strain which was vaccine strain for the 2008-2009 season. Conclusions
The oseltamivir-resistant A(H1N1) viruses were first emerged in Europe in November 2007 and then circulated globally. One year later, the oseltamivir-resistant A(H1N1) viruses were first detected in Korea in November 2008 and continued circulating until the Week 7 of 2009 during the 2008-2009 season. Considering the pandemic preparedness, it should be continued to monitor the emergence and the characterization of antiviral drug resistant influenza viruses.
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- Pharmacokinetics and safety of a novel influenza treatment (baloxavir marboxil) in Korean subjects compared with Japanese subjects
Yun Kim, Sangwon Lee, Yohan Kim, In‐Jin Jang, SeungHwan Lee Clinical and Translational Science.2022; 15(2): 422. CrossRef - 2018–2019 antiviral drug sensitivity of the influenza virus strains isolated from various regions of Kazakhstan
T. I. Glebova, N. G. Klivleyeva, G. V. Lukmanova, N. T. Saktaganov, A. M. Baimukhametova Russian Journal of Infection and Immunity.2021; 11(6): 1159. CrossRef - Assessment of Intensive Vaccination and Antiviral Treatment in 2009 Influenza Pandemic in Korea
Chaeshin Chu, Sunmi Lee Osong Public Health and Research Perspectives.2015; 6(1): 47. CrossRef - Doing Mathematics with Aftermath of Pandemic Influenza 2009
Hae-Wol Cho, Chaeshin Chu Osong Public Health and Research Perspectives.2015; 6(1): 1. CrossRef - Antiviral treatment of influenza in South Korea
Young June Choe, Hyunju Lee, Hoan Jong Lee, Eun Hwa Choi Expert Review of Anti-infective Therapy.2015; 13(6): 741. CrossRef - Synthesis and anti-influenza virus activity of 4-oxo- or thioxo-4,5-dihydrofuro[3,4-c]pyridin-3(1H)-ones
Ye Jin Jang, Raghavendra Achary, Hye Won Lee, Hyo Jin Lee, Chong-Kyo Lee, Soo Bong Han, Young-Sik Jung, Nam Sook Kang, Pilho Kim, Meehyein Kim Antiviral Research.2014; 107: 66. CrossRef - Was the Mass Vaccination Effective During the Influenza Pandemic 2009–2010 in Korea?
Hae-Wol Cho, Chaeshin Chu Osong Public Health and Research Perspectives.2013; 4(4): 177. CrossRef - How to Manage a Public Health Crisis and Bioterrorism in Korea
Hae-Wol Cho, Chaeshin Chu Osong Public Health and Research Perspectives.2013; 4(5): 223. CrossRef - Generation and Characterization of Recombinant Influenza A(H1N1) Viruses Resistant to Neuraminidase Inhibitors
WooYoung Choi, Jin-Young Shin, Hwan-Eui Jeong, Mi-Jin Jeong, Su-Jin Kim, Joo-Yeon Lee, Chun Kang Osong Public Health and Research Perspectives.2013; 4(6): 323. CrossRef - Occurrence and characterization of oseltamivir-resistant influenza virus in children between 2007-2008 and 2008-2009 seasons
Seoung Geun Kim, Yoon Ha Hwang, Yung Hae Shin, Sung Won Kim, Woo Sik Jung, Sung Mi Kim, Jae Min Oh, Na Young Lee, Mun Ju Kim, Kyung Soon Cho, Yeon Gyeong Park, Sang Kee Min, Chang Kyu Lee, Jun Sub Kim, Chun Kang, Joo Yeon Lee, Man Kyu Huh, Chang Hoon Kim Korean Journal of Pediatrics.2013; 56(4): 165. CrossRef
- Pathogenesis and Chronologic Localization of the Human Influenza A (H1N1) Virus in Cotton Rats
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Donghyok Kwon, Kyeongcheol Shin, Jin-Young Shin, Joo-Yeon Lee, Yooncheol Ha, Nam-Joo Lee, Hee-Bok Oh, Chanhee Chae, Chun Kang
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Osong Public Health Res Perspect. 2011;2(1):15-22. Published online June 30, 2011
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DOI: https://doi.org/10.1016/j.phrp.2011.04.005
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3,563
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Abstract
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- Objectives
We aimed to evaluate the pathogenesis and chronologic localization of human influenza A (H1N1) virus in experimentally infected cotton rats. Methods
The animals were intranasally inoculated with 107 plaque-forming units of A/Solomon Islands/3/2006 (H1N1) influenza virus and evaluated for pathogenicity for a period of 28 days. Virus replication kinetics and pathological properties were assessed chronologically. Acute antiviral responses were evaluated by mean of real-time polymerase chain reaction. Results
Cotton rats infected with A/Solomon Islands/3/2006 virus lost weight until 6 days post-inoculation (DPI) and showed decreased activity until 3 DPI. At necropsy, focal areas of redness and consolidation of lungs were evident at 1, 2, and 3 DPI. Lung histopathology showed moderate to severe interstitial pneumonia, alveolitis and bronchiolitis. Influenza A specific viral protein was detected in bronchiolar epithelial cells, alveolar septa and pneumocytes. Influenza viruses were recovered from the lungs during the early period of infection and the titer peaked at 1 DPI. Viral proteins were detected from 4 hours to 6 hours DPI. These trends correlate with the up-regulation of mRNA expression of the IFN-α, Mx1, and Mx2 genes that play critical roles in the anti-influenza response at the early stage of infection. Conclusion
Our results provide evidence that supports the use of cotton rats for the study of influenza virus pathogenesis and the immune response.
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- Characterization of humoral immune responses and degree of protection induced by influenza vaccine in cotton rats: Effects of low vaccine dose and single vs booster vaccination
Yoshita Bhide, Wei Dong, Tjarko Meijerhof, Jacqueline de Vries‐Idema, Hubert G. Niesters, Anke Huckriede Immunity, Inflammation and Disease.2020; 8(3): 279. CrossRef - Doing Mathematics with Aftermath of Pandemic Influenza 2009
Hae-Wol Cho, Chaeshin Chu Osong Public Health and Research Perspectives.2015; 6(1): 1. CrossRef - Assessment of Intensive Vaccination and Antiviral Treatment in 2009 Influenza Pandemic in Korea
Chaeshin Chu, Sunmi Lee Osong Public Health and Research Perspectives.2015; 6(1): 47. CrossRef - Mammalian pathogenesis of oseltamivir-resistant pandemic (H1N1) 2009 influenza virus isolated in South Korea
Donghyok Kwon, Kyeongcheol Shin, Su-Jin Kim, Joo-Yeon Lee, Chun Kang Virus Research.2014; 185: 41. CrossRef - Was the Mass Vaccination Effective During the Influenza Pandemic 2009–2010 in Korea?
Hae-Wol Cho, Chaeshin Chu Osong Public Health and Research Perspectives.2013; 4(4): 177. CrossRef - How to Manage a Public Health Crisis and Bioterrorism in Korea
Hae-Wol Cho, Chaeshin Chu Osong Public Health and Research Perspectives.2013; 4(5): 223. CrossRef - The Road Less Traveled
Chaeshin Chu Osong Public Health and Research Perspectives.2011; 2(1): 1. CrossRef
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