- Inorganic Phosphorus and Potassium Are Putative Indicators of Delayed Separation of Whole Blood
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Jae-Eun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, Jin-Sook Wang, Haewon Shin, Jong-Wan Kim, Bok-Ghee Han, So-Youn Shin
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Osong Public Health Res Perspect. 2016;7(2):90-95. Published online April 30, 2016
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DOI: https://doi.org/10.1016/j.phrp.2015.11.003
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Abstract
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- Objectives
The delayed separation of whole blood can influence the concentrations of circulating blood components, including metabolites and cytokines. The aim of this study was to determine whether clinical-biochemistry analytes can be used to assess the delayed separation of whole blood. Methods
We investigated the plasma and serum concentrations of five clinical-biochemistry analytes and free hemoglobin when the centrifugation of whole blood stored at 4°C or room temperature was delayed for 4 hours, 6 hours, 24 hours, or 48 hours, and compared the values with those of matched samples that had been centrifuged within 2 hours after whole-blood collection. Results
The inorganic phosphorus (IP) levels in the plasma and serum samples were elevated ≥ 1.5-fold when whole-blood centrifugation was delayed at room temperature for 48 hours. Furthermore, the IP levels in the plasma samples showed excellent assessment accuracy [area under the receiver-operating-characteristic curve (AUC) > 0.9] after a 48-hour delay in whole-blood separation, and high sensitivity (100%) and specificity (95%) at an optimal cutoff point. The IP levels in the serum samples also exhibited good assessment accuracy (AUC > 0.8), and high sensitivity (81%) and specificity (100%). The potassium (K+) levels were elevated 1.4-fold in the serum samples following a 48-hour delay in whole-blood separation. The K+ levels showed excellent assessment accuracy (AUC > 0.9) following a 48-hour delay in whole-blood separation, and high sensitivity (95%) and specificity (91%) at an optimal cutoff point. Conclusion
Our study showed that the IP and K+ levels in the plasma or serum samples could be considered as putative indicators to determine whether whole-blood separation had been delayed for extended periods.
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- Assessing the stability of uncentrifuged serum and plasma analytes at various post-collection intervals
Atiqah Mokhsin, Poonaresi Subramaniam, Sivasooriar Sivaneson, Nelson Nheu, Gobhy Ramaloo, Azana S. Hanifah, Sumitha B. Mahathevan, Mohanaraja Nadarajah, Gayathiri Sampasivam, Aletza Mohd Ismail, Thuhairah Abdul Rahman Journal of Laboratory Medicine.2024;[Epub] CrossRef - Concordance in COVID-19 serology, bone mineralization, and inflammatory analytes between venous and self-collected capillary blood samples exposed to various pre-analytical conditions
Banafshe Hosseini, Harika Dasari, Anna Smyrnova, Claude Bourassa, Jing Leng, Christian Renaud, Francine M Ducharme Annals of Clinical Biochemistry: International Jou.2023; 60(4): 259. CrossRef - Assessment of avian health status: suitability and constraints of the Zoetis VetScan VS2 blood analyser for ecological and evolutionary studies
Ye Xiong, Michael Tobler, Arne Hegemann, Dennis L. Hasselquist Biology Open.2023;[Epub] CrossRef - Blood Plasma Quality Control by Plasma Glutathione Status
Tamara Tomin, Natalie Bordag, Elmar Zügner, Abdullah Al-Baghdadi, Maximilian Schinagl, Ruth Birner-Gruenberger, Matthias Schittmayer Antioxidants.2021; 10(6): 864. CrossRef - Identification of specific pre-analytical quality control markers in plasma and serum samples
Luz Ruiz-Godoy, Virginia Enríquez-Cárcamo, Lourdes Suárez-Roa, María Lourdes Lopez-Castro, Abel Santamaría, Mario Orozco-Morales, Ana Laura Colín-González Analytical Methods.2019; 11(17): 2259. CrossRef - Proteomics and Lipidomics in Inflammatory Bowel Disease Research: From Mechanistic Insights to Biomarker Identification
Bjoern Titz, Raffaella M. Gadaleta, Giuseppe Lo Sasso, Ashraf Elamin, Kim Ekroos, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng International Journal of Molecular Sciences.2018; 19(9): 2775. CrossRef - Impact of Preanalytical Variations in Blood-Derived Biospecimens on Omics Studies: Toward Precision Biobanking?
Jae-Eun Lee, Young-Youl Kim OMICS: A Journal of Integrative Biology.2017; 21(9): 499. CrossRef - Effect of delayed centrifugation of whole blood on serum samples stability
Massimo Daves, Vincenzo Roccaforte, Michele Giacomi, Monica Riva, Maria Leitner, Stefan Platzgummer, Gertraud Goetsch, Giuseppe Lippi La Rivista Italiana della Medicina di Laboratorio .2017; 13(1): 41. CrossRef - Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim Osong Public Health and Research Perspectives.2016; 7(6): 351. CrossRef
- Modification of AxSYM Human Immunodeficiency Virus Assay to Identify Recent Human Immunodeficiency Virus Infections in Korean Human Immunodeficiency Virus-Positive Individuals
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Jin-Sook Wang, Mee-Kyung Kee, Byeong-Sun Choi, Sung Soon Kim
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Osong Public Health Res Perspect. 2015;6(3):184-191. Published online June 30, 2015
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DOI: https://doi.org/10.1016/j.phrp.2015.06.002
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3,163
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Abstract
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- Objectives
To estimate human immunodeficiency virus (HIV) incidence using HIV avidity assays in Korea, we established a serological testing method to differentiate recent HIV infections from long-standing ones. Methods
We adopted two incidence assays, the BED HIV-1 incidence test (Calypte Biomedical) and an HIV avidity assay (using Abbott AxSYM HIV Antigen/Antibody Combo), and performed them on Korean HIV samples obtained from 81 HIV seroconverters (n = 193), 135 HIV-positive samples, and three HIV commercial incidence panels (PRB965, PRB933, and PRB601 from SeaCare). To determine the most optimal concentration of the chaotropic agent (Guanidine) and the cutoff value for the avidity assay, we evaluated the sensitivity and specificity of the assay at different concentration levels. Results
We determined that the concentration of Guanidine to be used in the avidity assay was 1.5M. The cutoff value of the avidity index (AI) was 0.8, and the sensitivity and specificity were 90.2% and 83.8%, respectively, under this condition. The gray zone for the avidity assay was 0.75–0.85 AI. The mean of coefficient of variation was low, at 5.43%. Conclusion
An optimized avidity assay for the diagnosis of recent HIV infections using Korean samples was established. This assay will be applied to investigate the level of recent infection and will provide basic data to the HIV prevention policy in Korea.
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Citations
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- Characteristics of recent HIV infection among individuals newly diagnosed as HIV-positive in South Korea (2008–2015)
Myeongsu Yoo, Jin-Sook Wang, Su-Jin Park, Jeong-ok Cha, Yoonhee Jung, Yoon-Seok Chung, Myung Guk Han, Byeong-Sun Choi, Sung-Soon Kim, Mee-Kyung Kee Scientific Reports.2022;[Epub] CrossRef
- The Recency Period for Estimation of Human Immunodeficiency Virus Incidence by the AxSYM Avidity Assay and BED-Capture Enzyme Immunoassay in the Republic of Korea
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Hye-Kyung Yu, Tae-Young Heo, Na-Young Kim, Jin-Sook Wang, Jae-Kyeong Lee, Sung Soon Kim, Mee-Kyung Kee
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Osong Public Health Res Perspect. 2014;5(4):187-192. Published online August 31, 2014
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DOI: https://doi.org/10.1016/j.phrp.2014.06.002
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3,247
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Abstract
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- Objectives
Measurement of the incidence of the human immunodeficiency virus (HIV) is very important for epidemiological studies. Here, we determined the recency period with the AxSYM avidity assay and the BED-capture enzyme immunoassay (BED-CEIA) in Korean seroconverters. Methods
Two hundred longitudinal specimens from 81 seroconverters with incident HIV infections that had been collected at the Korea National Institute of Health were subjected to the AxSYM avidity assay (cutoff = 0.8) and BED-CEIA (cutoff = 0.8). The statistical method used to estimate the recency period in recent HIV infections was nonparametric survival analyses. Sensitivity and specificity were calculated for 10-day increments from 120 days to 230 days to determine the recency period. Results
The mean recency period of the avidity assay and BED-CEIA using a survival method was 158 days [95% confidence interval (CI), 135–181 days] and 189 days (95% CI, 170–208 days), respectively. Based on the use of sensitivity and specificity, the mean recency period for the avidity assay and BED-CEIA was 150 days and 200 days, respectively. Conclusion
We determined the recency period to estimate HIV incidence in Korea. These data showed that the nonparametric survival analysis often led to shorter recency periods than analysis of sensitivity and specificity as a new method. These findings suggest that more data from seroconverters and other methodologies are needed to determine the recency period for estimating HIV incidence.
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Citations
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- Western Blot-Based Logistic Regression Model for the Identification of Recent HIV-1 Infection: A Promising HIV-1 Surveillance Approach for Resource-Limited Regions
Jiegang Huang, Minlian Wang, Chunyuan Huang, Bingyu Liang, Junjun Jiang, Chuanyi Ning, Ning Zang, Hui Chen, Jie Liu, Rongfeng Chen, Yanyan Liao, Li Ye, Hao Liang BioMed Research International.2018; 2018: 1. CrossRef
- Immune Status and Epidemiological Characteristics of Human Immunodeficiency Virus Seroconverters in Korea, 1999–2009
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Jin-Sook Wang, Na-young Kim, Hyo Jung Sim, Byeong-Sun Choi, Mee-Kyung Kee
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Osong Public Health Res Perspect. 2012;3(4):245-249. Published online December 31, 2012
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DOI: https://doi.org/10.1016/j.phrp.2012.11.001
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3,224
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Abstract
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- Objectives
The detection of HIV seroconverters increased annually since HIV antigen/antibody testing kits have been available widely in South Korea. This study aimed to identify the epidemiological characteristics of HIV seroconverters and their immune level at HIV diagnosis. Method
We analyzed the epidemiological and immunological characteristics of 341 HIV seroconverters among 6,008 HIV-diagnosed individuals from 1999 and 2009. The analysis of immune level and epidemiological factors of HIV seroconverters was conducted by using chi-square test on SAS version 9.1. Results
The seroconverters among newly-identified HIV cases each year increased from 0.5% in 1999 to over 5% or in 2009. The sex ratio of seroconverters was 18:1 (male:female), and 33% were in their 30s, and 28% were in their 20s. Reasons for HIV testing were involvement in voluntary test due to risky behaviors (43%), and health check-up (36%). Discovery of HIV infection occurred primarily in hospitals (84%). Among seroconverters, 55 percent had a CD4 T-cell count of more than 350/μl. Conclusion
Korean HIV seroconverters tended to be younger at diagnosis, diagnosed during a voluntary test, and their CD4+ T-cell counts at HIV diagnosis were higher than those of non-seroconverters aall HIV-infected individuals. This study of HIV seroconverters will be important foundational in future studies on HIV incidence, disease progress, and survival rate.
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Citations
Citations to this article as recorded by
- Characteristics of recent HIV infection among individuals newly diagnosed as HIV-positive in South Korea (2008–2015)
Myeongsu Yoo, Jin-Sook Wang, Su-Jin Park, Jeong-ok Cha, Yoonhee Jung, Yoon-Seok Chung, Myung Guk Han, Byeong-Sun Choi, Sung-Soon Kim, Mee-Kyung Kee Scientific Reports.2022;[Epub] CrossRef - Evaluation of the Bio-Rad Geenius HIV 1/2 Confirmation Assay as an Alternative to Western Blot in the Korean Population: A Multi-Center Study
Hee-Won Moon, Hee Jin Huh, Gwi Young Oh, Sang Gon Lee, Anna Lee, Yeo-Min Yun, Mina Hur, Herman Tse PLOS ONE.2015; 10(9): e0139169. CrossRef - Interferon-inducible protein 10 (IP-10) is associated with viremia of early HIV-1 infection in Korean patients
SoYong Lee, Yoon-Seok Chung, Cheol-Hee Yoon, YoungHyun Shin, SeungHyun Kim, Byeong-Sun Choi, Sung Soon Kim Journal of Medical Virology.2015; 87(5): 782. CrossRef
- Epidemiological and Immunological Characteristics at the Time of HIV Diagnosis for HIV/AIDS Cohort Registrants Representative of HIV-Infected Populations in Korea
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Jin-Hee Lee, Seung Hyun Kim, Jin-Sook Wang, Kyoung Mi Sung, Sung Soon Kim, Mee-Kyung Kee
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Osong Public Health Res Perspect. 2012;3(2):100-106. Published online June 30, 2012
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DOI: https://doi.org/10.1016/j.phrp.2012.04.002
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3,292
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- Objectives
The Korea HIV/AIDS cohort was constructed with 18 hospitals that care for HIV-infected individuals in 2006. We compared the epidemiological and immunological characteristics of the cohort registrants with those of the HIVinfected population at the time of HIV diagnosis. Methods
This study was conducted on 5717 people living with HIV/AIDS from 1985 to 2009, of which 789 individuals registered with the Korea HIV/AIDS cohort study. Individuals who had data from initial CD4+ T-cell counts measured within 6 months following HIV diagnosis were selected as study participants to predict the status of disease progression at the time of HIV diagnosis. A total of 2886 patients (50%) were selected from people living with HIV/AIDS, of whom 424 individuals (54%) were cohort registrants. The χ2 test and Wilcoxon rank sum test were used for analysis. Results
The distributions of age, marital status, diagnosed regions, reason for HIV testing, and screening site were similar between the HIV-infected population and the cohort registrants. In 1985–2004, the male ratio for the cohort registrants (94.3%) was significantly higher than that measured for the HIV-infected population (89.5%) (p = 0.0339). With regard to transmission route, homosexual contact of cohort registrants (46.6%) was higher than that of the HIV-infected population (40.1%) (p = 0.022) in 2005–2009. No statistical difference in CD4+ T-cell counts at the time of HIV diagnosis was found between the HIVinfected population and cohort registrants (p = 0.2195). Conclusion
The Korea HIV/AIDS cohort registrants represent the HIV-infected population, and the data collected from this cohort could be used as a foundation for national statistics.
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- Changing Patterns of HIV Epidemic in 30 Years in East Asia
S. Pilar Suguimoto, Teeranee Techasrivichien, Patou Masika Musumari, Christina El-saaidi, Bhekumusa Wellington Lukhele, Masako Ono-Kihara, Masahiro Kihara Current HIV/AIDS Reports.2014; 11(2): 134. CrossRef - Can Stigma Still Distort the Spectrum of a Disease?
Hae-Wol Cho, Chaeshin Chu Osong Public Health and Research Perspectives.2012; 3(2): 65. CrossRef
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