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Original Article

Multiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

Jie Yeun Park, Semi Jeon, Jun Young Kim, Misun Park, Seonghan Kim

Osong Public Health Res Perspect. 2013;4(3):133-139. Published online June 30, 2013

Cited By 14

Objectives

A multiplex real-time polymerase chain reaction (RT-PCR) method was developed for the identification of three Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus.

Methods

Specific primers and probes targeting the hlyA, tlh, and vvhA genes were selected and used for multiplex real-time PCR to confirm the identification of V. cholerae, V. parahaemolyticus, and V. vulnificus, respectively. This method was applied to...

DOI: https://doi.org/10.1016/j.phrp.2013.04.004

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Print ISSN: 2210-9099
Online ISSN: 2233-6052

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