- Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea
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Jin-Won Kim, Minji Lee, Hwachul Shin, Chi-Hwan Choi, Myung-Min Choi, Jee Woong Kim, Hwajung Yi, Cheon-Kwon Yoo, Gi-Eun Rhie
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Osong Public Health Res Perspect. 2022;13(4):308-311. Published online August 31, 2022
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DOI: https://doi.org/10.24171/j.phrp.2022.0232
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 Graphical Abstract
 Abstract
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 - Objectives
Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the patient’s specimens.
Methods
Clinical specimens from the patient were inoculated in Vero E6 cells. The isolated virus was identified as MPXV by the observation of cytopathic effects on Vero E6 cells, transmission electron microscopy, conventional polymerase chain reaction
(PCR), and sequencing of PCR products.
Results
Cytopathic effects were observed in Vero E6 cells that were inoculated with skin lesion swab eluates. After multiple passages from the primary culture, orthopoxvirus morphology was observed using transmission electron microscopy. In addition, both MPXV-specific (F3L and ATI) and orthopoxvirus-specific genes (A39R, B2R, and HA) were confirmed by conventional PCR and Sanger sequencing.
Conclusion
These results indicate the successful isolation and identification of MPXV from the first patient in the Republic of Korea. The isolated virus was named MPXV-ROK-P1-2022.
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Citations
Citations to this article as recorded by  - Ultrasensitive one-pot detection of monkeypox virus with RPA and CRISPR in a sucrose-aided multiphase aqueous system
Yue Wang, Yixin Tang, Yukang Chen, Guangxi Yu, Xue Zhang, Lihong Yang, Chenjie Zhao, Pei Wang, Song Gao, Frederick S. B. Kibenge, Ruijie Deng, Wei Chen, Shuang Yang
Microbiology Spectrum.2024;[Epub] CrossRef - Molecular Methods for Diagnosis of Monkeypox: A Mini-review
Rodrigo Michelini de Oliveira Thomasi, Thais da Silva Correa, Dalise Silva do Carmo, Déborah Fernandes Rodrigues, Luiz Vinicius da Silva Correa, Sandra Rodrigues Xavier, Líria Souza Silva, Jonatas Oliveira da Silva, Michelli dos Santos, Alessandra da Silv
Current Molecular Medicine.2024; 24(10): 1208. CrossRef - Epidemiological, Clinical, and Virological Investigation of the First Four Cases of Monkeypox in Cartagena during the 2022 Outbreak
Steev Loyola, Mashiel Fernández-Ruiz, Doris Gómez-Camargo
Pathogens.2023; 12(2): 159. CrossRef - 원숭이두창바이러스의 분리 배양과 전장유전체 정보 분석
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Public Health Weekly Report.2023; 16(15): 464. CrossRef - Overview of Diagnostic Methods, Disease Prevalence and Transmission of Mpox (Formerly Monkeypox) in Humans and Animal Reservoirs
Ravendra P. Chauhan, Ronen Fogel, Janice Limson
Microorganisms.2023; 11(5): 1186. CrossRef - How to cope with suspected mpox patients in the outpatient clinic
Nam Joong Kim, Sun Huh
Journal of the Korean Medical Association.2023; 66(5): 325. CrossRef - An Updated Review on Monkeypox Viral Disease: Emphasis on Genomic Diversity
Ali Rabaan, Nada Alasiri, Mohammed Aljeldah, Abeer Alshukairiis, Zainab AlMusa, Wadha Alfouzan, Abdulmonem Abuzaid, Aref Alamri, Hani Al-Afghani, Nadira Al-baghli, Nawal Alqahtani, Nadia Al-baghli, Mashahed Almoutawa, Maha Mahmoud Alawi, Mohammed Alabdull
Biomedicines.2023; 11(7): 1832. CrossRef - Monkeypox (Mpox) virus isolation and ultrastructural characterisation from a Brazilian human sample case
Milene Dias Miranda, Gabriela Cardoso Caldas, Vivian Neuza Ferreira, Ortrud Monika Barth, Aline de Paula Dias da Silva, Mayara Secco Torres Silva, Beatriz Grinsztejn, Valdiléa Gonçalves Veloso, Thiago Moreno Souza, Edson Elias da Silva, Debora Ferreira Ba
Memórias do Instituto Oswaldo Cruz.2023;[Epub] CrossRef - Isolation and Characterization of Monkeypox Virus from the First Case of Monkeypox — Chongqing Municipality, China, 2022
Baoying Huang, Hua Zhao, Jingdong Song, Li Zhao, Yao Deng, Wen Wang, Roujian Lu, Wenling Wang, Jiao Ren, Fei Ye, Houwen Tian, Guizhen Wu, Hua Ling, Wenjie Tan
China CDC Weekly.2022; 4(46): 1019. CrossRef
- Identification of Coronavirus Isolated from a Patient in Korea with COVID-19
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Jeong-Min Kim, Yoon-Seok Chung, Hye Jun Jo, Nam-Joo Lee, Mi Seon Kim, Sang Hee Woo, Sehee Park, Jee Woong Kim, Heui Man Kim, Myung-Guk Han
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Osong Public Health Res Perspect. 2020;11(1):3-7. Published online February 28, 2020
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DOI: https://doi.org/10.24171/j.phrp.2020.11.1.02
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55,208
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Abstract
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Objectives
Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.
Methods
Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.
Results
The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.
Conclusion
SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.
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