Jasia Bokhari | 1 Article |
<b>Objectives</b><br/>
This study was conducted to investigate the antioxidant potential of methanol extract and its derived fractions (hexane, ethyl acetate, butanol, and aqueous) of fruits of <i>Monotheca buxifolia</i> (Falc.) Dc., a locally used fruit in Pakistan.<br/><b>Methods</b><br/>
Dried powder of the fruit of <i>M. buxifolia</i> was extracted with methanol and the resultant was fractionated with solvents having escalating polarity; <i>n</i>-hexane, chloroform, ethyl acetate, <i>n</i>-butanol and the residual soluble aqueous fraction. Total phenolic and total flavonoid contents were estimated for the methanol and various fractions. These fractions were also subjected to various <i>in vitro</i> assays to estimate the scavenging activity for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide, hydroxyl, hydrogen peroxide and reductive ability for ferric ions and phosphomolybdate assay.<br/><b>Results</b><br/>
The <i>n</i>-butanol, aqueous and methanol fractions possessed high amount of phenolics and flavonoids compared with other fractions, and subsequently showed a pronounced scavenging activity on DPPH, ABTS, superoxide, hydroxyl and hydrogen peroxide radicals and had a potent reductive ability on ferric ion and phosphomolybdate assay. There was a found significant correlation between total phenolic and flavonoid contents and EC<sub>50</sub> of DPPH, superoxide, hydrogen peroxide radical and phosphomolybdate assays, whereas a nonsignificant correlation was found with the hydroxyl radical and ABTS radical assay.<br/><b>Conclusion</b><br/>
<i>M. buxifolia</i> fruit can be used as natural antioxidant source to prevent damage associated with free radicals.
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