Osong Public Health and Research Perspectives

Jae-Eun Lee

7 Articles

How Should Biobanks Collect Biosamples for Clinical Application? A 20-year Biomarker-related Publication and Patent Trend Analysis: Jae-Eun Lee; Osong Public Health Res Perspect. 2018;9(3):105-111. Published online June 30, 2018; DOI: https://doi.org/10.24171/j.phrp.2018.9.3.04

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Objectives

This study was designed to analyze biomarker-related publications and patent trends which biobanks could consider in planning biosample collections for biomarker research.

Methods

Publications and patents containing the term “biomarker” in the title published between 1998 to 2017 were retrieved using Scopus database and Google Patents search engine.

Results

Over the last 20 years there has been a steady increase in biomarker-related publications and patents; however this has slowed for patents over the last few years. Publications in 2017 that contained blood, serum, and plasma search terms in the abstract accounted for 50%, and serum as a search term in the title and abstract was more numerous than those containing blood, plasma, tissue, or urine. Blood-related patents were the most common patent in the last 10 years, and accounted for 110 patents in 2017. Biomarker-related publications since 2010 containing RNA and protein search terms in the title and abstract, were more numerous than those containing DNA and metabolite search terms. More than 27% of biomarker-related publications in 2017 and 21% of biomarker-related patents were associated with cancer.

Conclusion

The results of this study will help biobanks establish a biosample collection strategy for clinical application.

Citations

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Nanodiamond Solid-Phase Extraction and Triton X-114 Cloud Point Separation for Robust Fractionation and Shotgun Proteomics Analysis of the Human Serum Proteome
Hai Lam Khuong, Chein-Hung Chen, Jung-Lee Lin, Trong-Nghia Le, Thi Hue Pham, Thi Bich Thao Le, Xuan Canh Nguyen, Van Chi Phan, Hoang Ha Chu, Wesley Wei-Wen Hsiao, Thi Minh Phuong Nguyen, Dinh Minh Pham
Journal of Proteome Research.2022; 21(1): 67. CrossRef
Sampling strategies to evaluate the prognostic value of a new biomarker on a time-to-event end-point
Francesca Graziano, Maria Grazia Valsecchi, Paola Rebora
BMC Medical Research Methodology.2021;[Epub] CrossRef
MicroRNA-181b Serves as a Circulating Biomarker and Regulates Inflammation in Heart Failure
Hongxiao Yang, Lina Shan, Yunan Gao, Lin Li, Guifen Xu, Bin Wang, Xiaoxue Yin, Chengfang Gao, Jiaren Liu, Wei Yang, Gaetano Isola
Disease Markers.2021; 2021: 1. CrossRef
Enhancing Cooperation Between Academic Biobanks and Biomedical Industry: Better Mutual Understanding and New Collaborative Models Are Needed
Daniel Simeon-Dubach, Michael H. Roehrl, Paul Hofman, Pascal Puchois
Biopreservation and Biobanking.2020; 18(2): 144. CrossRef

Impact of Time Delay in Processing Blood Sample on Next Generation Sequencing for Transcriptome Analysis: Jae-Eun Lee, So-Young Jung, So-Youn Shin, Young-Youl Kim; Osong Public Health Res Perspect. 2018;9(3):130-132. Published online June 30, 2018; DOI: https://doi.org/10.24171/j.phrp.2018.9.3.09

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Effects of processing conditions on stability of immune analytes in human blood
Andres Gottfried-Blackmore, Samuel J. S. Rubin, Lawrence Bai, Sheun Aluko, Yujun Yang, Walter Park, Aida Habtezion
Scientific Reports.2020;[Epub] CrossRef

Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing: Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim; Osong Public Health Res Perspect. 2016;7(6):351-355. Published online December 31, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.11.004

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Abstract PDF

Objectives
Progastrin-releasing peptide (proGRP) is a promising biomarker for small cell lung cancer. However, not much is known about how sample processing and storage conditions affect the stability of proGRP. Here, we examined the effects of repeated freeze–thaw cycles on the stability of proGRP in plasma and serum.
Methods
Concentrations of proGRP were measured in plasma and serum samples exposed to two, three, or four freeze–thaw cycles and these were compared with values of corresponding samples exposed to one cycle (baseline). We also performed the area under the receiver-operating-characteristic curve (AUC) analysis to determine whether the differences of proGRP concentrations between each paired plasma and serum sample (ΔproGRP) can be used for identifying the samples that have been exposed to multiple freeze–thaw cycles.
Results
Concentrations of proGRP gradually decreased in both plasma and serum samples with increasing numbers of freeze–thaw cycles. Reduction rates of proGRP concentrations were greater in serum than in plasma samples and serum proGRP levels declined with statistical significance (p < 0.001) up to 10.1% after four freeze–thaw cycles. The ΔproGRP measurement showed fair accuracy (AUC = 0.741) for identifying samples that had been through four freeze–thaw cycles. The sensitivity was 82.8% and specificity was 62.1% at an optimal cut-off point of > 4.9.
Conclusion
Our study shows that the stability of circulating proGRP is affected in both plasma and serum samples by repeated freezing and thawing. We also show that ΔproGRP could be used for identifying paired plasma and serum samples subjected to multiple freeze–thaw cycles.

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Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
Alessandro Torelli, Elena Gianchecchi, Martina Monti, Pietro Piu, Irene Barneschi, Carolina Bonifazi, Rosa Coluccio, Luisa Ganfini, Luciano Michele La Magra, Silvia Marconi, Ginevra Marzucchi, Ramona Pace, Laura Palladino, Bernardo Biagi, Emanuele Montomo
Vaccines.2021; 9(3): 267. CrossRef
The influence of different blood samples treatment methods on pro-gastrin-releasing peptide
Huiqin Jiang, Ling Luo, Kang Xiong, Chengwen He, Huaizhou Wang, Yanghua Qin
Medicine.2019; 98(26): e16130. CrossRef

Inorganic Phosphorus and Potassium Are Putative Indicators of Delayed Separation of Whole Blood: Jae-Eun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, Jin-Sook Wang, Haewon Shin, Jong-Wan Kim, Bok-Ghee Han, So-Youn Shin; Osong Public Health Res Perspect. 2016;7(2):90-95. Published online April 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2015.11.003

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Abstract PDF

Objectives
The delayed separation of whole blood can influence the concentrations of circulating blood components, including metabolites and cytokines. The aim of this study was to determine whether clinical-biochemistry analytes can be used to assess the delayed separation of whole blood.
Methods
We investigated the plasma and serum concentrations of five clinical-biochemistry analytes and free hemoglobin when the centrifugation of whole blood stored at 4°C or room temperature was delayed for 4 hours, 6 hours, 24 hours, or 48 hours, and compared the values with those of matched samples that had been centrifuged within 2 hours after whole-blood collection.
Results
The inorganic phosphorus (IP) levels in the plasma and serum samples were elevated ≥ 1.5-fold when whole-blood centrifugation was delayed at room temperature for 48 hours. Furthermore, the IP levels in the plasma samples showed excellent assessment accuracy [area under the receiver-operating-characteristic curve (AUC) > 0.9] after a 48-hour delay in whole-blood separation, and high sensitivity (100%) and specificity (95%) at an optimal cutoff point. The IP levels in the serum samples also exhibited good assessment accuracy (AUC > 0.8), and high sensitivity (81%) and specificity (100%). The potassium (K⁺) levels were elevated 1.4-fold in the serum samples following a 48-hour delay in whole-blood separation. The K⁺ levels showed excellent assessment accuracy (AUC > 0.9) following a 48-hour delay in whole-blood separation, and high sensitivity (95%) and specificity (91%) at an optimal cutoff point.
Conclusion
Our study showed that the IP and K⁺ levels in the plasma or serum samples could be considered as putative indicators to determine whether whole-blood separation had been delayed for extended periods.

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Blood Plasma Quality Control by Plasma Glutathione Status
Tamara Tomin, Natalie Bordag, Elmar Zügner, Abdullah Al-Baghdadi, Maximilian Schinagl, Ruth Birner-Gruenberger, Matthias Schittmayer
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Identification of specific pre-analytical quality control markers in plasma and serum samples
Luz Ruiz-Godoy, Virginia Enríquez-Cárcamo, Lourdes Suárez-Roa, María Lourdes Lopez-Castro, Abel Santamaría, Mario Orozco-Morales, Ana Laura Colín-González
Analytical Methods.2019; 11(17): 2259. CrossRef
Proteomics and Lipidomics in Inflammatory Bowel Disease Research: From Mechanistic Insights to Biomarker Identification
Bjoern Titz, Raffaella Gadaleta, Giuseppe Lo Sasso, Ashraf Elamin, Kim Ekroos, Nikolai Ivanov, Manuel Peitsch, Julia Hoeng
International Journal of Molecular Sciences.2018; 19(9): 2775. CrossRef
Impact of Preanalytical Variations in Blood-Derived Biospecimens on Omics Studies: Toward Precision Biobanking?
Jae-Eun Lee, Young-Youl Kim
OMICS: A Journal of Integrative Biology.2017; 21(9): 499. CrossRef
Effect of delayed centrifugation of whole blood on serum samples stability
Massimo Daves, Vincenzo Roccaforte, Michele Giacomi, Monica Riva, Maria Leitner, Stefan Platzgummer, Gertraud Goetsch, Giuseppe Lippi
La Rivista Italiana della Medicina di Laboratorio .2017; 13(1): 41. CrossRef
Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
Osong Public Health and Research Perspectives.2016; 7(6): 351. CrossRef

Effect of Repeated Freezing and Thawing on Biomarker Stability in Plasma and Serum Samples: Jae-Eun Lee, Shine Young Kim, So-Youn Shin; Osong Public Health Res Perspect. 2015;6(6):357-362. Published online December 31, 2015; DOI: https://doi.org/10.1016/j.phrp.2015.11.005

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Abstract PDF

Objectives
The stability of circulating proteins can be affected by repeated freezing and thawing. The aim of our study was to identify the effect of repeated freezing and thawing on the plasma and serum concentrations of eight proteins [interferon-γ, interleukin (IL)-8, IL-15, IL-17A, matrix metalloproteinase (MMP)-7, tumor necrosis factor-α, vascular endothelial growth factor (VEGF), and VEGF receptor 2 (VEGF-R2)].
Methods
We assessed the concentration changes of these proteins in 30 plasma and serum samples subjected to three, four, or five freeze–thaw cycles, and compared these with the concentration changes in the samples that were subjected to two freeze–thaw cycles before analysis.
Results
Repeated freezing and thawing by up to five cycles did not modify the plasma and serum concentrations of interferon-γ, IL-8, and VEGF-R2, while levels of MMP-7, tumor necrosis factor-α, and VEGF were significantly changed in both plasma and serum samples. Moreover, MMP-7 and VEGF concentrations tended to increase with freeze–thaw cycles. They were more elevated in plasma samples (up to about 15%) than in serum samples (up to about 7%), suggesting that serum is the preferred sample type for the analysis of circulating proteins.
Conclusion
This is the first report on the effect of repeated freezing and thawing on plasma concentrations of MMP-7 and VEGF-R2. Our findings propose that researchers should consider the number of freeze–thaw cycles to select plasma or serum samples, depending on the type of analyte.

Citations

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Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart
Megha Bhardwaj, Petra Schrotz-King, Hermann Brenner
Life.2022; 12(3): 359. CrossRef
Development of quinone linked immunosorbent assay (QuLISA) based on using Folin’s reagent as a non-enzymatic tag: Application to analysis of food allergens
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Sensors and Actuators B: Chemical.2022; 368: 132167. CrossRef
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The effect of pre-analytical handling on the stability of fractalkine, monocyte chemoattractant protein 1 (MCP1), interleukin 6 and interleukin 8 in samples of human cerebrospinal fluid
Winnie Charlotte Pedersen Mortensen, Laila Bendix, Hanne Irene Jensen, Claus Varnum, Lasse Enkebølle Rasmussen, Jørgen T. Lauridsen, Nis Borbye-Lorenzen, Kristin Skogstrand, Palle Toft, Henrik Bjarke Vaegter, Morten Rune Blichfeldt-Eckhardt
Journal of Immunological Methods.2021; 494: 113057. CrossRef
Standardizing salivary lactoferrin measurements to obtain a robust diagnostic biomarker for Alzheimer's disease
Fernando Bartolome, Gorka Orive, Eva Carro
Alzheimer's & Dementia: Diagnosis, Assessment & Di.2021;[Epub] CrossRef
Biobanking for Neurodegenerative Diseases: Challenge for Translational Research and Data Privacy
Emilia Giannella, Valentino Notarangelo, Caterina Motta, Giulia Sancesario
The Neuroscientist.2021; : 107385842110366. CrossRef
Pre-analytical considerations in biomarker research: focus on cardiovascular disease
Elena Revuelta-López, Jaume Barallat, Adriana Cserkóová, Carolina Gálvez-Montón, Allan S. Jaffe, James L. Januzzi, Antoni Bayes-Genis
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Susan Tuddenham, Christina A. Stennett, Richard A. Cone, Jacques Ravel, Andrew N. Macintyre, Khalil G. Ghanem, Xin He, Rebecca M. Brotman
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Proximity Extension Assay in Combination with Next-Generation Sequencing for High-throughput Proteome-wide Analysis
Lotta Wik, Niklas Nordberg, John Broberg, Johan Björkesten, Erika Assarsson, Sara Henriksson, Ida Grundberg, Erik Pettersson, Christina Westerberg, Elin Liljeroth, Adam Falck, Martin Lundberg
Molecular & Cellular Proteomics.2021; 20: 100168. CrossRef
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Mario Plebani, Giuseppe Banfi, Sergio Bernardini, Francesco Bondanini, Laura Conti, Romolo Dorizzi, Fulvio Enrico Ferrara, Rita Mancini, Tommaso Trenti
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Association Between Intravenous Fluid Bolus and Biomarker Trajectory During Prehospital Care
Emily B. Brant, Jason Kennedy, Christian Martin-Gill, Vanessa Jackson, Octavia M. Peck Palmer, Clifton W. Callaway, Jeremy M. Kahn, Donald M. Yealy, Derek C. Angus, Christopher W. Seymour
Prehospital Emergency Care.2020; 24(2): 196. CrossRef
Evaluating sample stability in the clinical laboratory with the help of linear and non-linear regression analysis
Joachim K.W. Pum
Clinical Chemistry and Laboratory Medicine (CCLM).2020; 58(2): 188. CrossRef
Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal Reflux
Young Ju Lee, Jiyoon Kwon, Sanggyeong Shin, Young-Gyu Eun, Jae Ho Shin, Gi-Ja Lee
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Stability of 12 T-helper cell-associated cytokines in human serum under different pre-analytical conditions
Ulrik Stervbo, Sharon Bajda, Patrizia Wehler, Benjamin J. Rohn, Melanie Streichhahn, Sehriban Temizsoy, Eva Kohut, Toralf Roch, Richard Viebahn, Timm H. Westhoff, Nina Babel
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Mahmoud El-Maghrabey, Naoya Kishikawa, Shiori Harada, Kaname Ohyama, Naotaka Kuroda
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Standardized approaches for clinical sampling and endpoint ascertainment in tuberculous meningitis studies
Ursula K Rohlwink, Felicia C Chow, Sean Wasserman, Sofiati Dian, Rachel PJ Lai, Lidya Chaidir, Raph L Hamers, Robert J Wilkinson, David R Boulware, Fiona V Cresswell, Arjan van Laarhoven
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James M. Cameron, Holly J. Butler, David J. Anderson, Loren Christie, Lily Confield, Katie E. Spalding, Duncan Finlayson, Stuart Murray, Zanib Panni, Christopher Rinaldi, Alexandra Sala, Ashton G. Theakstone, Matthew J. Baker
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Francesco Bisognin, Giulia Lombardi, Maria Carla Re, Paola Dal Monte, Geoffrey A. Land
Journal of Clinical Microbiology.2020;[Epub] CrossRef
Stability of serum biochemical markers during standard long-term storage and with a single thawing
V. A. Kozlova, V. A. Metelskaya, M. S. Pokrovskaya, I. A. Efimova, O. A. Litinskaya, V. A. Kutsenko, E. B. Yarovaya, S. A. Shalnova, O. M. Drapkina
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Development and application of a porcine specific ELISA for the quantification of soluble CD163
J. Alex Pasternak, Daniel J. MacPhee, John C.S. Harding
Veterinary Immunology and Immunopathology.2019; 210: 60. CrossRef
Dynamics of circulating TNF during adalimumab treatment using a drug-tolerant TNF assay
Lea C. Berkhout, Merel J. l’Ami, Jill Ruwaard, Margreet H. Hart, Pleuni Ooijevaar-de Heer, Karien Bloem, Michael T. Nurmohamed, Ronald F. van Vollenhoven, Maarten Boers, Daniel F. Alvarez, Catherine H. Smith, Gerrit J. Wolbink, Theo Rispens
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Standardized approaches for clinical sampling and endpoint ascertainment in tuberculous meningitis studies
Ursula K Rohlwink, Felicia C Chow, Sean Wasserman, Sofiati Dian, Rachel PJ Lai, Lidya Chaidir, Raph L Hamers, Robert J Wilkinson, David R Boulware, Fiona V Cresswell, Arjan van Laarhoven
Wellcome Open Research.2019; 4: 204. CrossRef
The Effect of Anticoagulants, Temperature, and Time on the Human Plasma Metabolome and Lipidome from Healthy Donors as Determined by Liquid Chromatography-Mass Spectrometry
Manoj Khadka, Andrei Todor, Kristal M. Maner-Smith, Jennifer K. Colucci, ViLinh Tran, David A. Gaul, Evan J. Anderson, Muktha S. Natrajan, Nadine Rouphael, Mark J. Mulligan, Circe E. McDonald, Mehul Suthar, Shuzhao Li, Eric A. Ortlund
Biomolecules.2019; 9(5): 200. CrossRef
A caspase-6-cleaved fragment of Glial Fibrillary Acidic Protein as a potential serological biomarker of CNS injury after cardiac arrest
Ditte S. Jonesco, Christian Hassager, Martin Frydland, Jesper Kjærgaard, Morten Karsdal, Kim Henriksen, Stephen D. Ginsberg
PLOS ONE.2019; 14(11): e0224633. CrossRef
Angiogenic Factor and Cytokine Analysis among Patients Treated with Adjuvant VEGFR TKIs in Resected Renal Cell Carcinoma
Wenxin Xu, Maneka Puligandla, Judith Manola, Andrea J. Bullock, Daniel Tamasauskas, David F. McDermott, Michael B. Atkins, Naomi B. Haas, Keith Flaherty, Robert G. Uzzo, Janice P. Dutcher, Robert S. DiPaola, Rupal S. Bhatt
Clinical Cancer Research.2019; 25(20): 6098. CrossRef
Stability of important antibodies for kidney disease: pre-analytic methodological considerations
Qiuxia Han, Songyan Li, Bo Fu, Dongwei Liu, Maoqing Wu, Xiaoli Yang, Guangyan Cai, Zhangsuo Liu, Xiangmei Chen, Hanyu Zhu
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The association of angiogenic factors and chronic kidney disease
Christopher E. Anderson, L. Lee Hamm, Gem Batuman, Damodar R. Kumbala, Chung-Shiuan Chen, Swapna G. Kallu, Ravi Siriki, Shilpa Gadde, Myra A. Kleinpeter, N. Kevin Krane, Eric E. Simon, Jiang He, Jing Chen
BMC Nephrology.2018;[Epub] CrossRef
Strong impact on plasma protein profiles by precentrifugation delay but not by repeated freeze-thaw cycles, as analyzed using multiplex proximity extension assays
Qiujin Shen, Johan Björkesten, Joakim Galli, Daniel Ekman, John Broberg, Niklas Nordberg, Annika Tillander, Masood Kamali-Moghaddam, Gunnel Tybring, Ulf Landegren
Clinical Chemistry and Laboratory Medicine (CCLM).2018; 56(4): 582. CrossRef
Plasma-Derived Inflammatory Proteins Predict Oral Squamous Cell Carcinoma
Kelly Yi Ping Liu, Xian Jun David Lu, Yuqi Sarah Zhu, Nhu Le, Hugh Kim, Catherine F. Poh
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Nicola Goodfellow, Julien Morlet, Surjeet Singh, Afsie Sabokbar, Andrew Hutchings, Vanshika Sharma, Jana Vaskova, Shauna Masters, Allahdad Zarei, Raashid Luqmani
RMD Open.2017; 3(1): e000353. CrossRef
Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations
Arne Hegemann, Sara Pardal, Kevin D. Matson
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The Enhanced Liver Fibrosis (ELF) Panel: Analyte Stability Under Common Sample Storage Conditions Used in Clinical Practice
Oliver J Kennedy, Julie Parkes, Sudeep Tanwar, Paul M Trembling, William M Rosenberg
The Journal of Applied Laboratory Medicine.2017; 1(6): 720. CrossRef
Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
Osong Public Health and Research Perspectives.2016; 7(6): 351. CrossRef
Effects of Long-Term Storage Time and Original Sampling Month on Biobank Plasma Protein Concentrations
Stefan Enroth, Göran Hallmans, Kjell Grankvist, Ulf Gyllensten
EBioMedicine.2016; 12: 309. CrossRef

Instability at Short Tandem Repeats in Lymphoblastoid Cell Lines: Jae-Eun Lee, Eun-Jung Hong, Ji-Hyun Kim, So Youn Shin, Young-Youl Kim, Bok-Ghee Han; Osong Public Health Res Perspect. 2013;4(4):194-196. Published online August 31, 2013; DOI: https://doi.org/10.1016/j.phrp.2013.06.003

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Abstract PDF

Objectives
Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs) are a useful biological resource, however, genomic variations can happen during the generation and immortalization processes of LCLs. The purpose of this study was to identify genomic variations in LCL DNA compared with matched blood DNA using short tandem repeats (STRs) analysis.
Methods
We analyzed 15 STRs with blood DNA and their matched LCL DNA samples from 6645 unrelated healthy individuals.
Results
Mutations (such as repeat variations and triallelic patterns) of 15 STR loci were detected in 612 LCL DNAs (9.2% of total) without mutations in their matched blood DNA. The repeat variations of 15 STRs were detected in 526 LCL DNAs (mutation rate = 0.0792) and triallelic patterns were identified in 123 (mutation rate = 0.0185). Among 15 STRs, the most common repeat variations (n = 214, mutation rate = 0.0322) and triallelic patterns (n = 17, mutation rate = 0.0026) were found at FGA locus.
Conclusion
Our study shows that mutations in STRs can occur during generation and immortalization of LCLs.

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Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line
Christopher Korch, Erin M. Hall, Wilhelm G. Dirks, Margaret Ewing, Mark Faries, Marileila Varella-Garcia, Steven Robinson, Douglas Storts, Jacqueline A. Turner, Ying Wang, Edward C. Burnett, Lyn Healy, Douglas Kniss, Richard M. Neve, Raymond W. Nims, Yvon
International Journal of Cancer.2018; 142(3): 561. CrossRef

National Biobank of Korea: Quality control Programs of Collected-human Biospecimens: Jae-Eun Lee, Ji-Hyun Kim, Eun-Jung Hong, Hye Sook Yoo, Hye-Young Nam, Ok Park; Osong Public Health Res Perspect. 2012;3(3):185-189. Published online June 30, 2012; DOI: https://doi.org/10.1016/j.phrp.2012.07.007

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Abstract PDF

Personalized medicine is emerging as a main paradigm for risk prediction, pre-diagnosis, and effective prevention and treatment of disease. A large number of human biospecimens and their clinical data are essential resources for the success of personalized medicine as well as other biomedical research. The National Biobank of Korea (NBK) has collected well-annotated and high quality human biospecimens, and distributes them to the Korean biomedical scientists, through the Korea Biobank Project (KBP). The ultimate goal of NBK activities is to promote biomedical research and public health. As of December- 2011, the NBK has collected various human biospecimens from 525,416 participants including 325,952 Korean populations and 199,464 patients. The purpose of this paper is to introduce the KBP and quality control programs for collection of human biospecimens with high quality of NBK.

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DeepVariant-on-Spark: Small-Scale Genome Analysis Using a Cloud-Based Computing Framework
Po-Jung Huang, Jui-Huan Chang, Hou-Hsien Lin, Yu-Xuan Li, Chi-Ching Lee, Chung-Tsai Su, Yun-Lung Li, Ming-Tai Chang, Sid Weng, Wei-Hung Cheng, Cheng-Hsun Chiu, Petrus Tang
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Wiktor Paskal, Adriana M. Paskal, Tomasz Dębski, Maciej Gryziak, Janusz Jaworowski
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Author index