Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives


Author index

Page Path
HOME > Articles and issues > Author index
Hwan-Eui Jeong 1 Article
Generation and Characterization of Recombinant Influenza A(H1N1) Viruses Resistant to Neuraminidase Inhibitors
WooYoung Choi, Jin-Young Shin, Hwan-Eui Jeong, Mi-Jin Jeong, Su-Jin Kim, Joo-Yeon Lee, Chun Kang
Osong Public Health Res Perspect. 2013;4(6):323-328.   Published online December 31, 2013
  • 2,845 View
  • 16 Download
  • 7 Crossref
AbstractAbstract PDF
To examine the effect of neuraminidase (NA) mutations on the NA inhibitor (NAI) resistance phenotype, the recombinant influenza A/Chungbuk/4448/2008(H1N1) virus isolated in South Korea during the 2008–2009 season was generated by reverse genetics.
Site-directed mutagenesis was introduced on the NA gene of A/Chungbuk/4448/2008(H1N1) virus, and a total of 23 single, double, and triple mutants were generated. Resistance phenotype of these recombinant viruses was determined by NA-inhibition (NAI) assays based on a fluorometric method using two NAIs (oseltamivir and zanamivir).
NA-inhibition assays showed that all the single and double mutants containing the Y275 except the single Y275-E119V mutant conferred important levels of resistance to oseltamivir, whereas all the single, double, and triple mutants containing the E119V mutation were associated with the resistance to zanamivir.
Considering the effect of mutations in NA gene on the resistance to NAIs, it is important to monitor the possible emergence and dissemination of multidrug-resistant variants in the human population due to amino acid changes at NA gene as well as to develop novel NAIs.


Citations to this article as recorded by  
  • Phylogenetic analysis and docking study of neuraminidase gene of influenza A/H1N1 viruses circulating in Iran from 2010 to 2019
    Sina Moeini, Atefeh Mohebbi, Behrokh Farahmand, Parvaneh Mehrbod, Fatemeh Fotouhi
    Virus Research.2023; 334: 199182.     CrossRef
    Kevin M. Downard
    Mass Spectrometry Reviews.2022; 41(1): 3.     CrossRef
  • Next-Generation Sequencing: An Eye-Opener for the Surveillance of Antiviral Resistance in Influenza
    Laura A.E. Van Poelvoorde, Xavier Saelens, Isabelle Thomas, Nancy H. Roosens
    Trends in Biotechnology.2020; 38(4): 360.     CrossRef
  • Mutation-induced spatial differences in neuraminidase structure and sensitivity to neuraminidase inhibitors
    Zhi-wei Yang, Dong-xiao Hao, Yi-zhuo Che, Jia-hui Yang, Lei Zhang, Sheng-li Zhang
    Chinese Physics B.2018; 27(1): 018704.     CrossRef
  • Authorised medicinal product Aspecton® Oral Drops containing thyme extract KMTv24497 shows antiviral activity against viruses which cause respiratory infections
    Eva Lenz, Christin Müller, Ahmed Mostafa, Julia Dzieciolowski, Pumaree Kanrai, Sharmistha Dam, Ute Cwientzek, Lars-Norbert Prenner, Stephan Pleschka
    Journal of Herbal Medicine.2018; 13: 26.     CrossRef
  • Ultrasensitive Fluorogenic Reagents for Neuraminidase Titration
    Zhizeng Gao, Masahiro Niikura, Stephen G. Withers
    Angewandte Chemie International Edition.2017; 56(22): 6112.     CrossRef
  • Ultrasensitive Fluorogenic Reagents for Neuraminidase Titration
    Zhizeng Gao, Masahiro Niikura, Stephen G. Withers
    Angewandte Chemie.2017; 129(22): 6208.     CrossRef

PHRP : Osong Public Health and Research Perspectives