- Ingenol Protects Human T Cells From HIV-1 Infection
-
Kee-Jong Hong, Hak Sung Lee, Yeong-shik Kim, Sung Soon Kim
-
Osong Public Health Res Perspect. 2011;2(2):109-114. Published online June 30, 2011
-
DOI: https://doi.org/10.1016/j.phrp.2011.07.001
-
-
1,946
View
-
17
Download
-
10
Citations
-
 Abstract
 PDF
- Objectives
Many natural compounds have been investigated as drug candidates to prevent human immunodeficiency virus (HIV) with low cytotoxicity. We tested whether ingenol from Euphorbia ingens exerts anti-HIV effects in human T cell lines.
Methods
and Results
Ingenol effectively maintained high cell viability (CD50, >1 mM) in H9 and MT4 T cells. The efficacy of ingenol to inhibit HIV-1 infection was dose dependent. ED50 for 100 and 200 TCID50 of HIV-1 was 5.06 and 16.87 μM, respectively. Gag p24 antigen production in ingenol-treated MT4 cells was reduced by 24.5% on day 6 post-infection. While p24 antigen was reduced in ingenol-treated cells, levels of cytokines such as TNF-α and IL-6 and chemokines such as RANTES and MCP-1 were increased. dUTP level related to late apoptotic events was increased on day 2 post-infection of HIV by ingenol treatment, whereas expression of annexin V was unchanged. Reduced levels of iNOS and ZAP-70 after HIV infection were recovered by ingenol treatment.
Conclusion
Ingenol helps T cells to survive longer against viremia after HIV-1 infection, without exerting cytotoxic effects. Ingenol can be considered a safe and efficacious candidate for immune-boosting therapy for AIDS patients.
-
Citations
Citations to this article as recorded by  - A review of the ethnomedicinal, pharmacology, cytotoxicity and phytochemistry of the genus Euphorbia in southern Africa
E.J. Mavundza, R. Street, H. Baijnath
South African Journal of Botany.2022; 144: 403. CrossRef - South African plants with nematicidal activity against root-knot nematodes: A review
F.N. Makhubu, M.C. Khosa, L.J. McGaw
South African Journal of Botany.2021; 139: 183. CrossRef - Systematic characterization of metabolic profiles of ingenol in rats by UPLC-Q/TOF-MS and NMR in combination with microbial biotransformation
Si-Jia Xiao, Shan-Shan Li, Bin Xie, Wei Chen, Xi-Ke Xu, Xian-Peng Zu, Yun-Heng Shen
RSC Advances.2021; 11(60): 37752. CrossRef - Structure-Activity-Relationship and Mechanistic Insights for Anti-HIV Natural Products
Ramandeep Kaur, Pooja Sharma, Girish K. Gupta, Fidele Ntie-Kang, Dinesh Kumar
Molecules.2020; 25(9): 2070. CrossRef - Modified ingenol semi-synthetic derivatives from Euphorbia tirucalli induce cytotoxicity on a large panel of human cancer cell lines
Viviane A. O Silva, Marcela N. Rosa, Olga Martinho, Amilcar Tanuri, João Paulo Lima, Luiz F. Pianowski, Rui M. Reis
Investigational New Drugs.2019; 37(5): 1029. CrossRef - Natural product-derived compounds in HIV suppression, remission, and eradication strategies
Raymond J. Andersen, Fidele Ntie-Kang, Ian Tietjen
Antiviral Research.2018; 158: 63. CrossRef - Biphasic Effects of Ingenol 3,20-Dibenzoate on the Erythropoietin Receptor: Synergism at Low Doses and Antagonism at High Doses
Jin-Gyo Oh, Young-Won Chin, Sung-Jo Kim, Jong Min Choi, Sang Kyum Kim, Hee Eun Kang, Tae-Hwe Heo
Molecular Pharmacology.2015; 88(2): 392. CrossRef - LC-MS2-Based dereplication of Euphorbia extracts with anti-Chikungunya virus activity
Louis-Félix Nothias-Scaglia, Vincent Dumontet, Johan Neyts, Fanny Roussi, Jean Costa, Pieter Leyssen, Marc Litaudon, Julien Paolini
Fitoterapia.2015; 105: 202. CrossRef - Reactivation of latent HIV-1 by new semi-synthetic ingenol esters
Diego Pandeló José, Koen Bartholomeeusen, Rodrigo Delvecchio da Cunha, Celina Monteiro Abreu, Jan Glinski, Thais Barbizan Ferreira da Costa, Ana Flávia Mello Bacchi Rabay, Luiz Francisco Pianowski Filho, Lech W. Dudycz, Udaykumar Ranga, Boris Matija Peter
Virology.2014; 462-463: 328. CrossRef - Dual Role of Novel Ingenol Derivatives from Euphorbia tirucalli in HIV Replication: Inhibition of De Novo Infection and Activation of Viral LTR
Celina M. Abreu, Sarah L. Price, Erin N. Shirk, Rodrigo D. Cunha, Luiz F. Pianowski, Janice E. Clements, Amilcar Tanuri, Lucio Gama, Cheryl A. Stoddart
PLoS ONE.2014; 9(5): e97257. CrossRef
|
