Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives


Author index

Page Path
HOME > Articles and issues > Author index
Goutam Ghosh 1 Article
In Vitro Antibacterial Activity, Gas Chromatography–Mass Spectrometry Analysis of Woodfordia fruticosa Kurz. Leaf Extract and Host Toxicity Testing With In Vitro Cultured Lymphocytes From Human Umbilical Cord Blood
Debasmita Dubey, Rajashree Patnaik, Goutam Ghosh, Rabindra N. Padhy
Osong Public Health Res Perspect. 2014;5(5):298-312.   Published online October 31, 2014
  • 3,326 View
  • 27 Download
  • 16 Crossref
AbstractAbstract PDF
To locate a plant with suitable phytochemicals for use as antimicrobial agents to control multidrug-resistant (MDR) bacteria as a complementary medicine, without host toxicity as monitored through cultured lymphocytes from human umbilical cord blood.
The methanol crude leaf extract of the plant Woodfordia fruticosa was subjected to antimicrobial assay in vitro with nine pathogenic MDR bacteria from clinical samples. This was followed by bioassay-guided fractionation with seven non-polar to polar solvents, gas chromatography–mass spectrometry analysis of the n-butanol fraction, and monitoring of the host toxicity of the leaf extract with in vitro grown lymphocytes from human umbilical cord blood.
The leaf extract of W. fruticosa had a controlling capacity for MDR bacteria. The minimum inhibitory concentration and minimum bactericidal concentration of the n-butanol fraction were < 1.89 mg/mL extract and 9.63 mg/mL extract, respectively. The gas chromatography–mass spectrometry spectrum of the n-butanol fraction confirmed the presence of 13 peaks of different compounds with retention times of 9.11 minutes, 9.72 minutes, 10.13 minutes, 10.78 minutes, 12.37 minutes, 12.93 minutes, 18.16 minutes, 21.74 minutes, 21.84 minutes, 5.96 minutes, 12.93 minutes, 24.70 minutes, and 25.76 minutes. The six leading compounds were: diethyl phthalate: IUPAC name: diethyl benzene-1,2-dicarboxylate; 5-methyl-2-(1-methylethyl) phenol: IUPAC name: 5-methyl-2-propan-2-ylphenol; (E )-3,7-dimethylocta-2,6-diene-1-thiol: IUPAC name: (2Z)-3,7-dimethylocta-2,6-diene-1-thiol; 2,6,10-dodecatrien-1-ol, 3,7,11-trimethyl-, (E,E ): IUPAC name: 2,6,10-dodecatrien-1-ol; 3,7,11-trimethyl-, (E,E); 2-methoxy-4-(2-propenyl) phenol: IUPAC name: 2-methoxy-4-[(1E)-prop-1-en-1-yl]phenol; hexadecanoic acid: IUPAC name: hexadecanoic acid.
The presence of antimicrobial compounds that are therapeutically potent against MDR bacteria was confirmed in W. fruticosa. The crude leaf extract showed no host toxicity with human lymphocytes; the n-butanol fraction of the extract was the most suitable bioactive fraction. The terpenes isolated were: 5-methyl-2-(1-methylethyl) phenol, 2-methoxy-4-(2-propenyl) phenol, 2,6-octadien-1-ol, 3,7-dimethyl-(E)-2,6-octadienal, 3,7-dimethylcyclohexanol, and cyclohexanol, 2-methylene-5-(1-methylethenyl) which were reported to have specifically antimicrobial activity.


Citations to this article as recorded by  
  • Facile Synthesis of Benzimidazoles via Oxidative Cyclization of Acyclic Monoterpene Aldehyde with Diamines: Studies on Antimicrobial and in Vivo Evaluation of Zebrafish
    Mariyappan Vaithiyalingam, Ramasamy Mohan Kumar, Chinnaperumal Kamaraj, Vimal Sugumar, Nandhagopal Manivannan, Shine Kadaikunnan, Gajanan Ghodake
    Chemistry & Biodiversity.2023;[Epub]     CrossRef
  • Woodfordia fruticosa (L.) Kurz: in vitro biotechnological interventions and perspectives
    Mahender Aileni, Mallesham Bulle, Ramesh Naik Malavath, Satyamraj Thurpu, Kiranmayi Bandaram, Bhargavi Balkampeta, Meghana Marri, Vijaya Simha Reddy Singasani, E. N. Murthy
    Applied Microbiology and Biotechnology.2023; 107(19): 5855.     CrossRef
  • Antibacterial activity of essential oils for combating colistin-resistant bacteria
    Abdullah M. Foda, Mohamed H. Kalaba, Gamal M. El-Sherbiny, Saad A. Moghannem, Esmail M. El-Fakharany
    Expert Review of Anti-infective Therapy.2022; 20(10): 1351.     CrossRef
  • Woodfordia fruticosa extract nanoemulsion: Influence of processing treatment on droplet size and its assessment for in vitro antimicrobial and anti-inflammatory activity
    Agnieszka Najda, Aarti Bains, Joanna Klepacka, Prince Chawla
    Frontiers in Nutrition.2022;[Epub]     CrossRef
  • Antioxidant-Rich Woodfordia fruticosa Leaf Extract Alleviates Depressive-Like Behaviors and Impede Hyperglycemia
    Mohammed Abu Tayab, Kazi Ashfak Ahmed Chowdhury, Md. Jabed, Syed Mohammed Tareq, A. T. M. Mostafa Kamal, Mohammad Nazmul Islam, A. M. Kafil Uddin, Mohammad Adil Hossain, Talha Bin Emran, Jesus Simal-Gandara
    Plants.2021; 10(2): 287.     CrossRef
  • Proteolytic enzyme arbitrated antagonization of helminthiasis by Cinnamomum cappara leaf extract in Pheretima posthuma
    Kayeen Vadakkan, Meena K Cheruvathur, Anu S Chulliparambil, Famy Francis, Anu P Abimannue
    Clinical Phytoscience.2021;[Epub]     CrossRef
  • Assessment of Anti-Inflammatory and Antimicrobial Potential of Ethanolic Extract of Woodfordia fruticosa Flowers: GC-MS Analysis
    Agnieszka Najda, Aarti Bains, Prince Chawla, Anil Kumar, Sebastian Balant, Magdalena Walasek-Janusz, Dariusz Wach, Ravinder Kaushik
    Molecules.2021; 26(23): 7193.     CrossRef
  • Plant-Based Phytochemicals as Possible Alternative to Antibiotics in Combating Bacterial Drug Resistance
    Hana Mohammed Al AlSheikh, Insha Sultan, Vijay Kumar, Irfan A. Rather, Hashem Al-Sheikh, Arif Tasleem Jan, Qazi Mohd Rizwanul Haq
    Antibiotics.2020; 9(8): 480.     CrossRef
  • Ethnomedicinal Uses of Plant Resources in the Machhapuchchhre Rural Municipality of Kaski District, Nepal
    Mahendra Adhikari, Rashmi Thapa, Ripu Mardhan Kunwar, Hari Prasad Devkota, Prakash Poudel
    Medicines.2019; 6(2): 69.     CrossRef
  • Screening of in vitro antimicrobial activity of plants used in traditional Indonesian medicine
    Andreas Romulo, Ervizal A. M. Zuhud, Johana Rondevaldova, Ladislav Kokoska
    Pharmaceutical Biology.2018; 56(1): 287.     CrossRef
  • GC MS Analysis of One Ayurvedic Preparation ‘Aswagandharishtam
    M. Kotteswari, M. R. K. Rao, Siva Kumar, K. Prabhu, R. Lakshmi Sundaram, Shruthi Dinakar
    Biomedical and Pharmacology Journal.2018; 11(2): 1061.     CrossRef
  • Phytochemical constituents, antioxidant and antibacterial activities of methanolic extract of Ardisia elliptica
    Nazeh M. Al-Abd, Zurainee Mohamed Nor, Marzida Mansor, Asdren Zajmi, Mohd Shahnaz Hasan, Fadzly Azhar, Mustafa Kassim
    Asian Pacific Journal of Tropical Biomedicine.2017; 7(6): 569.     CrossRef
  • In vitro antibacterial activity of crude extracts of 9 selected medicinal plants against UTI causing MDR bacteria
    Monali P. Mishra, Sibanarayan Rath, Shasank S. Swain, Goutam Ghosh, Debajyoti Das, Rabindra N. Padhy
    Journal of King Saud University - Science.2017; 29(1): 84.     CrossRef
  • Antiviral activity of selected medicinal plants and marine seaweeds on the grasserie infected larvae of silkworm, Bombyx mori
    Chitra Somu, Ramaraj Paulchamy, S. M. Moorthy, Janarthanan Sundaram
    Archives of Phytopathology and Plant Protection.2017; 50(17-18): 850.     CrossRef
  • Screening and antibacterial efficacy of selected Indian medicinal plants
    Suresh Mickymaray, Mohammad Saleh Al Aboody, Pradipta Kumar Rath, Panneerselvam Annamalai, Thajuddin Nooruddin
    Asian Pacific Journal of Tropical Biomedicine.2016; 6(3): 185.     CrossRef
  • Ellagic acid: Pharmacological activities and molecular mechanisms involved in liver protection
    Wylly Ramsés García-Niño, Cecilia Zazueta
    Pharmacological Research.2015; 97: 84.     CrossRef

PHRP : Osong Public Health and Research Perspectives