- Comparison of Four Serological Tests for Detecting Antibodies to Japanese Encephalitis Virus after Vaccination in Children
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Go Woon Cha, Jung Eun Cho, Young Ran Ju, Young-Jin Hong, Myung Guk Han, Won-Ja Lee, Eui Yul Choi, Young Eui Jeong
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Osong Public Health Res Perspect. 2014;5(5):286-291. Published online October 31, 2014
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DOI: https://doi.org/10.1016/j.phrp.2014.08.003
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5,024
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 Abstract
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- Objectives
Several different methods are currently used to detect antibodies to Japanese encephalitis virus (JEV) in serum samples or cerebrospinal fluid. These methods include the plaque reduction neutralization test (PRNT), the hemagglutination inhibition (HI) test, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA). The purpose of this study was to compare the performance of each method in detecting vaccine-induced antibodies to JEV.
Methods
The study included 29 children who had completed a primary immunization schedule with an inactivated vaccine against JEV derived from mouse brain (n = 15) or a live attenuated SA14-14-2 vaccine (n = 14). Serum samples were collected between 3 months and 47 months after the last immunization. The serum samples were tested by performing the PRNT, HI test, in-house IFA, and commercial ELISA. The antibody detection rates were compared between tests.
Results
All 29 serum samples were positive with the PRNT, showing antibody titers from 1:20 to 1:2560. The HI test showed positive rates of 86.7% (13/15) and 71.4% (10/14) in the inactivated and live attenuated vaccine groups, respectively. The results of the IFA for immunoglobulin (Ig)G were positive in 53.3% (8/15) of children in the inactivated vaccine group and 35.7% (5/14) in the live attenuated vaccine group. Neither the IFA nor ELISA detected JEV IgM antibodies in any of the 29 children.
Conclusion
These results show that detection rates of vaccine-induced antibodies to JEV have a wide range (0–100%) depending on the testing method as well as the time since immunization and individual differences between children. These findings are helpful in interpreting serological test results for the diagnosis of Japanese encephalitis in situations where vaccines are widely administered.
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Citations
Citations to this article as recorded by  - Comparative Analysis of Hemagglutination Inhibition and Plaque Reduction Neutralization Tests for Japanese Encephalitis Virus Antibody Detection
Cui Li, Jianqing Wan, Deli Wang, Lu Xiao, Xuni Li, Cunshuai Zhang, Zhao Wang
Viruses.2025; 17(1): 104. CrossRef - Development of Colloidal Gold-Based Immunochromatographic Strips for Rapid Detection and Surveillance of Japanese Encephalitis Virus in Dogs across Shanghai, China
Dengke Zhong, Abdul Wahaab, Jiayang Zheng, Junjie Zhang, Zhiyong Ma, Jianchao Wei
Viruses.2024; 16(2): 258. CrossRef - Laboratory evaluation of ELISA and indirect immunofluorescence assay in response to emergence of Japanese encephalitis virus genotype IV in Australia
Paul Kinsella, Michael Moso, Genevieve Martin, Theo Karapangiotidis, Di Karamalakis, Suellen Nicholson, Mitch Batty, Kathy Jackson, Madeleine Marsland, Tilda Thomson, Lakshmi Manoharan, Helen O'brien, N. Deborah Friedman, Katherine Bond, Deborah A. Willia
Journal of Clinical Virology.2023; 168: 105580. CrossRef - A review on Japanese Encephalitis virus emergence, pathogenesis and detection: From conventional diagnostics to emerging rapid detection techniques
Fatima Mohsin, Shariq Suleman, Nigar Anzar, Jagriti Narang, Shikha Wadhwa
International Journal of Biological Macromolecules.2022; 217: 435. CrossRef - Recent pharmaceutical engineered trends as theranostics for Japanese encephalitis
Akshada Mhaske, Sanjiv Singh, Mohammed A.S. Abourehab, Akhilesh Kumar, Prashant Kesharwani, Rahul Shukla
Process Biochemistry.2022; 122: 115. CrossRef - Immunogenicity of a single fractional intradermal dose of Japanese encephalitis live attenuated chimeric vaccine
Luis Furuya-Kanamori, Narayan Gyawali, Deborah J Mills, Christine Mills, Leon E Hugo, Gregor J Devine, Colleen L Lau
Journal of Travel Medicine.2022;[Epub] CrossRef - In silico molecular docking in DNA aptamer development
Tholasi Nadhan Navien, Ramesh Thevendran, Hazrina Yusof Hamdani, Thean-Hock Tang, Marimuthu Citartan
Biochimie.2021; 180: 54. CrossRef - Pathobiology of Japanese encephalitis virus infection
Kiran Bala Sharma, Sudhanshu Vrati, Manjula Kalia
Molecular Aspects of Medicine.2021; 81: 100994. CrossRef - JEV-nanobarcode and colorimetric reverse transcription loop-mediated isothermal amplification (cRT-LAMP)
Gna Ahn, Se Hee Lee, Min-Suk Song, Beom-Ku Han, Yang-Hoon Kim, Ji-Young Ahn
Microchimica Acta.2021;[Epub] CrossRef - Review of Emerging Japanese Encephalitis Virus: New Aspects and Concepts about Entry into the Brain and Inter-Cellular Spreading
Luis Filgueira, Nils Lannes
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Ji Hyen Lee, Han Wool Kim, Kyung-Hyo Kim
Pediatric Infection & Vaccine.2018; 25(3): 132. CrossRef - Japanese Encephalitis: A Brief Review on Indian Perspectives
Reshma Kulkarni, Gajanan N. Sapkal, Himanshu Kaushal, Devendra T. Mourya
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Kyung-Il Park, Manho Kim, Jun-Sang Sunwoo, Ki-Young Jung, Kon Chu, Keun-Hwa Jung, Sang Kun Lee, Soon-Tae Lee, Jangsup Moon
The American Journal of Tropical Medicine and Hygi.2017; 97(2): 369. CrossRef - JAPANESE ENCEPHALITIS, RECENT PERSPECTIVES ON VIRUS GENOME, TRANSMISSION, EPIDEMIOLOGY, DIAGNOSIS AND PROPHYLACTIC INTERVENTIONS
Arumugam Karthikeyan, Subramaniyan Shanmuganathan, Selvaraj Pavulraj, Govinthasamy Prabakar, Selvaraj Pavithra, Kannan Porteen, Govindaraj Elaiyaraja, Yashpal Singh Malik
Journal of Experimental Biology and Agricultural S.2017; 5(6): 730. CrossRef - Prevalence of Neutralizing Antibodies to Japanese Encephalitis Virus among High-Risk Age Groups in South Korea, 2010
Eun Ju Lee, Go-Woon Cha, Young Ran Ju, Myung Guk Han, Won-Ja Lee, Young Eui Jeong, Nagendra R Hegde
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Go-Woon Cha, Eun Ju Lee, Eun-Joo Lim, Kang Suk Sin, Woo Won Park, Doo Young Jeon, Myung Guk Han, Won-Ja Lee, Woo-Young Choi, Young Eui Jeong, Lark L. Coffey
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Maite Aubry, Jérôme Finke, Anita Teissier, Claudine Roche, Julien Broult, Sylvie Paulous, Philippe Desprès, Van-Mai Cao-Lormeau, Didier Musso
International Journal of Infectious Diseases.2015; 37: 19. CrossRef
- Travel-Associated Chikungunya Cases in South Korea during 2009–2010
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Go Woon Cha, Jung Eun Cho, Eun Ju Lee, Young Ran Ju, Myung Guk Han, Chan Park, Young Eui Jeong
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Osong Public Health Res Perspect. 2013;4(3):170-175. Published online June 30, 2013
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DOI: https://doi.org/10.1016/j.phrp.2013.04.008
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4,518
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27
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11
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Abstract
PDF
- Objectives
Chikungunya (CHIK) has been classified as a communicable disease group IV in South Korea since late 2010. Based on this, we investigated the extent of imported cases of CHIK in dengue-suspected individuals returning from dengue-endemic regions.
Methods
A total of 486 dengue-suspected serum samples were screened for CHIK by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Further RT-PCR-positive samples were used for the viral culture, and CHIK was subsequently confirmed by sequence analysis of the culture samples.
Results
Five out of 107 dengue-positive samples were found to be positive for CHIK and 15 out of 379 dengue-negative samples were found to be positive for CHIK by immunoglobulin M ELISA. Further, a CHIK virus was isolated from one of the two RT-PCR-positive sera by cell culture and confirmed by sequence analysis.
Conclusion
The present study documents the first evidence of travel-associated CHIK infection in South Korea. Considering the intense international traffic between countries, our finding emphasizes the urgent need for active patient and vector surveillance for timely response to reduce the introduction of CHIK in Korea.
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Citations
Citations to this article as recorded by - Metabarcoding for the Monitoring of the Microbiome and Parasitome of Medically Important Mosquito Species in Two Urban and Semi-urban Areas of South Korea
Xavier Chavarria, Jun Ho Choi, Singeun Oh, Myungjun Kim, Dongjun Kang, In-Yong Lee, Yun Soo Jang, Myung-hee Yi, Tai-Soon Yong, Ju Yeong Kim
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Ahmad Adebayo Irekeola, E.A. R Engku Nur Syafirah, Md Asiful Islam, Rafidah Hanim Shueb
Acta Tropica.2022; 231: 106408. CrossRef - Current Status and a Perspective of Mosquito-Borne Diseases in the Republic of Korea
Jae Hyoung Im, Tong-Soo Kim, Moon-Hyun Chung, Ji Hyeon Baek, Hea Yoon Kwon, Jin-Soo Lee
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Woo-Chang Chung, Kwang Yeon Hwang, Suk-Jo Kang, Jae-Ouk Kim, Moon Jung Song
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