- Comparison of Four Serological Tests for Detecting Antibodies to Japanese Encephalitis Virus after Vaccination in Children
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Go Woon Cha, Jung Eun Cho, Young Ran Ju, Young-Jin Hong, Myung Guk Han, Won-Ja Lee, Eui Yul Choi, Young Eui Jeong
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Osong Public Health Res Perspect. 2014;5(5):286-291. Published online October 31, 2014
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DOI: https://doi.org/10.1016/j.phrp.2014.08.003
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 Abstract
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- Objectives
Several different methods are currently used to detect antibodies to Japanese encephalitis virus (JEV) in serum samples or cerebrospinal fluid. These methods include the plaque reduction neutralization test (PRNT), the hemagglutination inhibition (HI) test, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA). The purpose of this study was to compare the performance of each method in detecting vaccine-induced antibodies to JEV.
Methods
The study included 29 children who had completed a primary immunization schedule with an inactivated vaccine against JEV derived from mouse brain (n = 15) or a live attenuated SA14-14-2 vaccine (n = 14). Serum samples were collected between 3 months and 47 months after the last immunization. The serum samples were tested by performing the PRNT, HI test, in-house IFA, and commercial ELISA. The antibody detection rates were compared between tests.
Results
All 29 serum samples were positive with the PRNT, showing antibody titers from 1:20 to 1:2560. The HI test showed positive rates of 86.7% (13/15) and 71.4% (10/14) in the inactivated and live attenuated vaccine groups, respectively. The results of the IFA for immunoglobulin (Ig)G were positive in 53.3% (8/15) of children in the inactivated vaccine group and 35.7% (5/14) in the live attenuated vaccine group. Neither the IFA nor ELISA detected JEV IgM antibodies in any of the 29 children.
Conclusion
These results show that detection rates of vaccine-induced antibodies to JEV have a wide range (0–100%) depending on the testing method as well as the time since immunization and individual differences between children. These findings are helpful in interpreting serological test results for the diagnosis of Japanese encephalitis in situations where vaccines are widely administered.
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Citations
Citations to this article as recorded by  - A review on Japanese Encephalitis virus emergence, pathogenesis and detection: From conventional diagnostics to emerging rapid detection techniques
Fatima Mohsin, Shariq Suleman, Nigar Anzar, Jagriti Narang, Shikha Wadhwa
International Journal of Biological Macromolecules.2022; 217: 435. CrossRef - Recent pharmaceutical engineered trends as theranostics for Japanese encephalitis
Akshada Mhaske, Sanjiv Singh, Mohammed A.S. Abourehab, Akhilesh Kumar, Prashant Kesharwani, Rahul Shukla
Process Biochemistry.2022; 122: 115. CrossRef - Immunogenicity of a single fractional intradermal dose of Japanese encephalitis live attenuated chimeric vaccine
Luis Furuya-Kanamori, Narayan Gyawali, Deborah J Mills, Christine Mills, Leon E Hugo, Gregor J Devine, Colleen L Lau
Journal of Travel Medicine.2022;[Epub] CrossRef - In silico molecular docking in DNA aptamer development
Tholasi Nadhan Navien, Ramesh Thevendran, Hazrina Yusof Hamdani, Thean-Hock Tang, Marimuthu Citartan
Biochimie.2021; 180: 54. CrossRef - Pathobiology of Japanese encephalitis virus infection
Kiran Bala Sharma, Sudhanshu Vrati, Manjula Kalia
Molecular Aspects of Medicine.2021; 81: 100994. CrossRef - JEV-nanobarcode and colorimetric reverse transcription loop-mediated isothermal amplification (cRT-LAMP)
Gna Ahn, Se Hee Lee, Min-Suk Song, Beom-Ku Han, Yang-Hoon Kim, Ji-Young Ahn
Microchimica Acta.2021;[Epub] CrossRef - Review of Emerging Japanese Encephalitis Virus: New Aspects and Concepts about Entry into the Brain and Inter-Cellular Spreading
Filgueira, Lannes
Pathogens.2019; 8(3): 111. CrossRef - Seroprevalence of Dengue Virus Antibody in Korea
Ji Hyen Lee, Han Wool Kim, Kyung-Hyo Kim
Pediatric Infection & Vaccine.2018; 25(3): 132. CrossRef - Japanese Encephalitis: A Brief Review on Indian Perspectives
Reshma Kulkarni, Gajanan N. Sapkal, Himanshu Kaushal, Devendra T. Mourya
The Open Virology Journal.2018; 12(1): 121. CrossRef - Clinical Characteristics of Severe Japanese Encephalitis: A Case Series from South Korea
Kyung-Il Park, Manho Kim, Jun-Sang Sunwoo, Ki-Young Jung, Kon Chu, Keun-Hwa Jung, Sang Kun Lee, Soon-Tae Lee, Jangsup Moon
The American Journal of Tropical Medicine and Hygi.2017; 97(2): 369. CrossRef - JAPANESE ENCEPHALITIS, RECENT PERSPECTIVES ON VIRUS GENOME, TRANSMISSION, EPIDEMIOLOGY, DIAGNOSIS AND PROPHYLACTIC INTERVENTIONS
Arumugam Karthikeyan, Subramaniyan Shanmuganathan, Selvaraj Pavulraj, Govinthasamy Prabakar, Selvaraj Pavithra, Kannan Porteen, Govindaraj Elaiyaraja, Yashpal Singh Malik
Journal of Experimental Biology and Agricultural S.2017; 5(6): 730. CrossRef - Prevalence of Neutralizing Antibodies to Japanese Encephalitis Virus among High-Risk Age Groups in South Korea, 2010
Eun Ju Lee, Go-Woon Cha, Young Ran Ju, Myung Guk Han, Won-Ja Lee, Young Eui Jeong, Nagendra R Hegde
PLOS ONE.2016; 11(1): e0147841. CrossRef - A Novel Immunochromatographic Test Applied to a Serological Survey of Japanese Encephalitis Virus on Pig Farms in Korea
Go-Woon Cha, Eun Ju Lee, Eun-Joo Lim, Kang Suk Sin, Woo Won Park, Doo Young Jeon, Myung Guk Han, Won-Ja Lee, Woo-Young Choi, Young Eui Jeong, Lark L. Coffey
PLOS ONE.2015; 10(5): e0127313. CrossRef - Silent Circulation of Ross River Virus in French Polynesia
Maite Aubry, Jérôme Finke, Anita Teissier, Claudine Roche, Julien Broult, Sylvie Paulous, Philippe Desprès, Van-Mai Cao-Lormeau, Didier Musso
International Journal of Infectious Diseases.2015; 37: 19. CrossRef
- Travel-Associated Chikungunya Cases in South Korea during 2009–2010
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Go Woon Cha, Jung Eun Cho, Eun Ju Lee, Young Ran Ju, Myung Guk Han, Chan Park, Young Eui Jeong
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Osong Public Health Res Perspect. 2013;4(3):170-175. Published online June 30, 2013
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DOI: https://doi.org/10.1016/j.phrp.2013.04.008
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2,232
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13
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10
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Abstract
PDF
- Objectives
Chikungunya (CHIK) has been classified as a communicable disease group IV in South Korea since late 2010. Based on this, we investigated the extent of imported cases of CHIK in dengue-suspected individuals returning from dengue-endemic regions.
Methods
A total of 486 dengue-suspected serum samples were screened for CHIK by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Further RT-PCR-positive samples were used for the viral culture, and CHIK was subsequently confirmed by sequence analysis of the culture samples.
Results
Five out of 107 dengue-positive samples were found to be positive for CHIK and 15 out of 379 dengue-negative samples were found to be positive for CHIK by immunoglobulin M ELISA. Further, a CHIK virus was isolated from one of the two RT-PCR-positive sera by cell culture and confirmed by sequence analysis.
Conclusion
The present study documents the first evidence of travel-associated CHIK infection in South Korea. Considering the intense international traffic between countries, our finding emphasizes the urgent need for active patient and vector surveillance for timely response to reduce the introduction of CHIK in Korea.
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Citations
Citations to this article as recorded by - Global prevalence of dengue and chikungunya coinfection: A systematic review and meta-analysis of 43,341 participants
Ahmad Adebayo Irekeola, E.A. R Engku Nur Syafirah, Md Asiful Islam, Rafidah Hanim Shueb
Acta Tropica.2022; 231: 106408. CrossRef - Current Status and a Perspective of Mosquito-Borne Diseases in the Republic of Korea
Jae Hyoung Im, Tong-Soo Kim, Moon-Hyun Chung, Ji Hyeon Baek, Hea Yoon Kwon, Jin-Soo Lee
Vector-Borne and Zoonotic Diseases.2021; 21(2): 69. CrossRef - Implications of a travel connectivity-based approach for infectious disease transmission risks in Oceania
Angela Cadavid Restrepo, Luis Furuya-Kanamori, Helen Mayfield, Eric Nilles, Colleen L Lau
BMJ Open.2021; 11(8): e046206. CrossRef - Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate
Woo-Chang Chung, Kwang Yeon Hwang, Suk-Jo Kang, Jae-Ouk Kim, Moon Jung Song
Journal of Microbiology.2020; 58(1): 46. CrossRef - Chikungunya virus infection in Indonesia: a systematic review and evolutionary analysis
Harapan Harapan, Alice Michie, Mudatsir Mudatsir, Roy Nusa, Benediktus Yohan, Abram Luther Wagner, R. Tedjo Sasmono, Allison Imrie
BMC Infectious Diseases.2019;[Epub] CrossRef - Global prevalence and distribution of coinfection of malaria, dengue and chikungunya: a systematic review
Nasir Salam, Shoeb Mustafa, Abdul Hafiz, Anis Ahmad Chaudhary, Farah Deeba, Shama Parveen
BMC Public Health.2018;[Epub] CrossRef - Chikungunya Virus Infection after Traveling to Surinam, South America
Hoe-Soo Jang, Jong-Hun Chung, Joa Kim, Sun Ae Han, Na-Ra Yun, Dong-Min Kim
The Korean Journal of Medicine.2016; 90(3): 262. CrossRef - Molecular epidemiology, evolution and phylogeny of Chikungunya virus: An updating review
Alessandra Lo Presti, Eleonora Cella, Silvia Angeletti, Massimo Ciccozzi
Infection, Genetics and Evolution.2016; 41: 270. CrossRef - The First Imported Case Infected with Chikungunya Virus in Korea
Jeong-Hwan Hwang, Chang-Seop Lee
Infection & Chemotherapy.2015; 47(1): 55. CrossRef - Zika virus in Brazil and the danger of infestation by Aedes (Stegomyia) mosquitoes
Carlos Brisola Marcondes, Maria de Fátima Freire de Melo Ximenes
Revista da Sociedade Brasileira de Medicina Tropic.2015; 49(1): 4. CrossRef
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