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Gi-Eun Rhie 5 Articles
Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea
Jin-Won Kim, Minji Lee, Hwachul Shin, Chi-Hwan Choi, Myung-Min Choi, Jee Woong Kim, Hwajung Yi, Cheon-Kwon Yoo, Gi-Eun Rhie
Osong Public Health Res Perspect. 2022;13(4):308-311.   Published online August 31, 2022
DOI: https://doi.org/10.24171/j.phrp.2022.0232
  • 1,162 View
  • 66 Download
  • 1 Citations
Graphical AbstractGraphical Abstract AbstractAbstract PDF
Objectives
Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the patient’s specimens.
Methods
Clinical specimens from the patient were inoculated in Vero E6 cells. The isolated virus was identified as MPXV by the observation of cytopathic effects on Vero E6 cells, transmission electron microscopy, conventional polymerase chain reaction (PCR), and sequencing of PCR products.
Results
Cytopathic effects were observed in Vero E6 cells that were inoculated with skin lesion swab eluates. After multiple passages from the primary culture, orthopoxvirus morphology was observed using transmission electron microscopy. In addition, both MPXV-specific (F3L and ATI) and orthopoxvirus-specific genes (A39R, B2R, and HA) were confirmed by conventional PCR and Sanger sequencing.
Conclusion
These results indicate the successful isolation and identification of MPXV from the first patient in the Republic of Korea. The isolated virus was named MPXV-ROK-P1-2022.

Citations

Citations to this article as recorded by  
  • Isolation and Characterization of Monkeypox Virus from the First Case of Monkeypox — Chongqing Municipality, China, 2022
    Baoying Huang, Hua Zhao, Jingdong Song, Li Zhao, Yao Deng, Wen Wang, Roujian Lu, Wenling Wang, Jiao Ren, Fei Ye, Houwen Tian, Guizhen Wu, Hua Ling, Wenjie Tan
    China CDC Weekly.2022; 4(46): 1019.     CrossRef
Genomic Surveillance of SARS-CoV-2: Distribution of Clades in the Republic of Korea in 2020
Ae Kyung Park, Il-Hwan Kim, Junyoung Kim, Jeong-Min Kim, Heui Man Kim, Chae young Lee, Myung-Guk Han, Gi-Eun Rhie, Donghyok Kwon, Jeong-Gu Nam, Young-Joon Park, Jin Gwack, Nam-Joo Lee, SangHee Woo, Jin Sun No, Jaehee Lee, Jeemin Ha, JeeEun Rhee, Cheon-Kwon Yoo, Eun-Jin Kim
Osong Public Health Res Perspect. 2021;12(1):37-43.   Published online February 23, 2021
DOI: https://doi.org/10.24171/j.phrp.2021.12.1.06
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  • 188 Download
  • 16 Citations
AbstractAbstract PDF

Since a novel beta-coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in December 2019, there has been a rapid global spread of the virus. Genomic surveillance was conducted on samples isolated from infected individuals to monitor the spread of genetic variants of SARS-CoV-2 in Korea. The Korea Disease Control and Prevention Agency performed whole genome sequencing of SARS-CoV-2 in Korea for 1 year (January 2020 to January 2021). A total of 2,488 SARS-CoV-2 cases were sequenced (including 648 cases from abroad). Initially, the prevalent clades of SARS-CoV-2 were the S and V clades, however, by March 2020, GH clade was the most dominant. Only international travelers were identified as having G or GR clades, and since the first variant 501Y.V1 was identified (from a traveler from the United Kingdom on December 22nd, 2020), a total of 27 variants of 501Y.V1, 501Y.V2, and 484K.V2 have been classified (as of January 25th, 2021). The results in this study indicated that quarantining of travelers entering Korea successfully prevented dissemination of the SARS-CoV-2 variants in Korea.

Citations

Citations to this article as recorded by  
  • Rapid Emergence of the Omicron Variant of Severe Acute Respiratory Syndrome Coronavirus 2 in Korea
    Ae Kyung Park, Il-Hwan Kim, Chae Young Lee, Jeong-Ah Kim, Hyeokjin Lee, Heui Man Kim, Nam-Joo Lee, SangHee Woo, Jaehee Lee, JeeEun Rhee, Cheon-Kwon Yoo, Eun-Jin Kim
    Annals of Laboratory Medicine.2023; 43(2): 211.     CrossRef
  • Genomic evidence of SARS‐CoV‐2 reinfection in the Republic of Korea
    Ae Kyung Park, Jee Eun Rhee, Il‐Hwan Kim, Heui Man Kim, Hyeokjin Lee, Jeong‐Ah Kim, Chae Young Lee, Nam‐Joo Lee, SangHee Woo, Jaehee Lee, Jin Sun No, Gi‐Eun Rhie, Seong Jin Wang, Sang‐Eun Lee, Young Joon Park, Gemma Park, Jung Yeon Kim, Jin Gwack, Cheon‐K
    Journal of Medical Virology.2022; 94(4): 1717.     CrossRef
  • SARS-CoV-2 B.1.619 and B.1.620 Lineages, South Korea, 2021
    Ae Kyung Park, Il-Hwan Kim, Heui Man Kim, Hyeokjin Lee, Nam-Joo Lee, Jeong-Ah Kim, SangHee Woo, Chae young Lee, Jaehee Lee, Sae Jin Oh, JeeEun Rhee, Cheon-Kwon Yoo, Eun-Jin Kim
    Emerging Infectious Diseases.2022; 28(2): 415.     CrossRef
  • Humoral and Cellular Responses to COVID-19 Vaccines in SARS-CoV-2 Infection-Naïve and -Recovered Korean Individuals
    Ji-Young Hwang, Yunhwa Kim, Kyung-Min Lee, Eun-Jeong Jang, Chang-Hoon Woo, Chang-Ui Hong, Seok-Tae Choi, Sivilay Xayaheuang, Jong-Geol Jang, June-Hong Ahn, Hosun Park
    Vaccines.2022; 10(2): 332.     CrossRef
  • Increase in Viral Load in Patients With SARS-CoV-2 Delta Variant Infection in the Republic of Korea
    Jeong-Min Kim, Jee Eun Rhee, Myeongsu Yoo, Heui Man Kim, Nam-Joo Lee, Sang Hee Woo, Hye-Jun Jo, Donghyok Kwon, Sangwon Lee, Cheon Kwon Yoo, Eun-Jin Kim
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • Molecular Dynamics Studies on the Structural Stability Prediction of SARS-CoV-2 Variants Including Multiple Mutants
    Kwang-Eun Choi, Jeong-Min Kim, Jee Eun Rhee, Ae Kyung Park, Eun-Jin Kim, Cheon Kwon Yoo, Nam Sook Kang
    International Journal of Molecular Sciences.2022; 23(9): 4956.     CrossRef
  • SARS-CoV-2 shedding dynamics and transmission in immunosuppressed patients
    Jee-Soo Lee, Ki Wook Yun, Hyeonju Jeong, Boram Kim, Man Jin Kim, Jae Hyeon Park, Ho Seob Shin, Hyeon Sae Oh, Hobin Sung, Myung Gi Song, Sung Im Cho, So Yeon Kim, Chang Kyung Kang, Pyoeng Gyun Choe, Wan Beom Park, Nam Joong Kim, Myoung-Don Oh, Eun Hwa Choi
    Virulence.2022; 13(1): 1242.     CrossRef
  • Immunological and Pathological Peculiarity of Severe Acute Respiratory Syndrome Coronavirus 2 Beta Variant
    Sunhee Lee, Gun Young Yoon, Su Jin Lee, Young-Chan Kwon, Hyun Woo Moon, Yu-Jin Kim, Haesoo Kim, Wooseong Lee, Gi Uk Jeong, Chonsaeng Kim, Kyun-Do Kim, Seong-Jun Kim, Dae-Gyun Ahn, Miguel Angel Martinez
    Microbiology Spectrum.2022;[Epub]     CrossRef
  • Clinical scoring system to predict viable viral shedding in patients with COVID-19
    Sung Woon Kang, Heedo Park, Ji Yeun Kim, Sunghee Park, So Yun Lim, Sohyun Lee, Joon-Yong Bae, Jeonghun Kim, Seongman Bae, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Sung-Cheol Yun, Man-Seong Park, Sung-Han Kim
    Journal of Clinical Virology.2022; 157: 105319.     CrossRef
  • Model-informed COVID-19 exit strategy with projections of SARS-CoV-2 infections generated by variants in the Republic of Korea
    Sung-mok Jung, Kyungmin Huh, Munkhzul Radnaabaatar, Jaehun Jung
    BMC Public Health.2022;[Epub]     CrossRef
  • Comparative analysis of mutational hotspots in the spike protein of SARS-CoV-2 isolates from different geographic origins
    Sanghoo Lee, Mi-Kyeong Lee, Hyeongkyun Na, Jinwoo Ahn, Gayeon Hong, Youngkee Lee, Jimyeong Park, Yejin Kim, Yun-Tae Kim, Chang-Ki Kim, Hwan-Sub Lim, Kyoung-Ryul Lee
    Gene Reports.2021; 23: 101100.     CrossRef
  • Review of Current COVID-19 Diagnostics and Opportunities for Further Development
    Yan Mardian, Herman Kosasih, Muhammad Karyana, Aaron Neal, Chuen-Yen Lau
    Frontiers in Medicine.2021;[Epub]     CrossRef
  • Locally harvested Covid-19 convalescent plasma could probably help combat the geographically determined SARS-CoV-2 viral variants
    Manish Raturi, Anuradha Kusum, Mansi Kala, Garima Mittal, Anita Sharma, Naveen Bansal
    Transfusion Clinique et Biologique.2021; 28(3): 300.     CrossRef
  • Molecular Dynamics Studies on the Structural Characteristics for the Stability Prediction of SARS-CoV-2
    Kwang-Eun Choi, Jeong-Min Kim, JeeEun Rhee, Ae Kyung Park, Eun-Jin Kim, Nam Sook Kang
    International Journal of Molecular Sciences.2021; 22(16): 8714.     CrossRef
  • Management following the first confirmed case of SARS-CoV-2 in a domestic cat associated with a massive outbreak in South Korea
    Taewon Han, Boyeong Ryu, Suyeon Lee, Yugyeong Song, Yoongje Jeong, Ilhwan Kim, Jeongmin Kim, Eunjin Kim, Wonjun Lee, Hyunju Lee, Haekyoung Hwang
    One Health.2021; 13: 100328.     CrossRef
  • Genomic epidemiology reveals the reduction of the introduction and spread of SARS-CoV-2 after implementing control strategies in Republic of Korea, 2020
    Jung-Hoon Kwon, Jeong-Min Kim, Dong-hun Lee, Ae Kyung Park, Il-Hwan Kim, Da-Won Kim, Ji-Yun Kim, Noori Lim, Kyeong-Yeon Cho, Heui Man Kim, Nam-Joo Lee, SangHee Woo, Chae Young Lee, Jin Sun No, Junyoung Kim, JeeEun Rhee, Myung-Guk Han, Gi-Eun Rhie, Cheon K
    Virus Evolution.2021;[Epub]     CrossRef
2019 Tabletop Exercise for Laboratory Diagnosis and Analyses of Unknown Disease Outbreaks by the Korea Centers for Disease Control and Prevention
Il-Hwan Kim, Jun Hyeong Jang, Su-Kyoung Jo, Jin Sun No, Seung-Hee Seo, Jun-Young Kim, Sang-Oun Jung, Jeong-Min Kim, Sang-Eun Lee, Hye-Kyung Park, Eun-Jin Kim, Jun Ho Jeon, Myung-Min Choi, Boyeong Ryu, Yoon Suk Jang, Hwami Kim, Jin Lee, Seung-Hwan Shin, Hee Kyoung Kim, Eun-Kyoung Kim, Ye Eun Park, Cheon-Kwon Yoo, Sang-Won Lee, Myung-Guk Han, Gi-Eun Rhie, Byung Hak Kang
Osong Public Health Res Perspect. 2020;11(5):280-285.   Published online October 22, 2020
DOI: https://doi.org/10.24171/j.phrp.2020.11.5.03
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AbstractAbstract PDF
Objectives

The Korea Centers for Disease Control and Prevention has published “A Guideline for Unknown Disease Outbreaks (UDO).” The aim of this report was to introduce tabletop exercises (TTX) to prepare for UDO in the future.

Methods

The UDO Laboratory Analyses Task Force in Korea Centers for Disease Control and Prevention in April 2018, assigned unknown diseases into 5 syndromes, designed an algorithm for diagnosis, and made a panel list for diagnosis by exclusion. Using the guidelines and laboratory analyses for UDO, TTX were introduced.

Results

Since September 9th, 2018, the UDO Laboratory Analyses Task Force has been preparing TTX based on a scenario of an outbreak caused by a novel coronavirus. In December 2019, through TTX, individual missions, epidemiological investigations, sample treatments, diagnosis by exclusions, and next generation sequencing analysis were discussed, and a novel coronavirus was identified as the causal pathogen.

Conclusion

Guideline and laboratory analyses for UDO successfully applied in TTX. Conclusions drawn from TTX could be applied effectively in the analyses for the initial response to COVID-19, an ongoing epidemic of 2019 – 2020. Therefore, TTX should continuously be conducted for the response and preparation against UDO.

Serological Correlate of Protection in Guinea Pigs for a Recombinant Protective Antigen Anthrax Vaccine Produced from Bacillus brevis
Jeong-Hoon Chun, On-Jee Choi, Min-Hee Cho, Kee-Jong Hong, Won Keun Seong, Hee-Bok Oh, Gi-Eun Rhie
Osong Public Health Res Perspect. 2012;3(3):170-176.   Published online June 30, 2012
DOI: https://doi.org/10.1016/j.phrp.2012.07.006
  • 2,179 View
  • 22 Download
  • 10 Citations
AbstractAbstract PDF
Objective Recombinant protective antigen (rPA) is the active pharmaceutical ingredient of a second generation anthrax vaccine undergoing clinical trials both in Korea and the USA. By using the rPA produced from Bacillus brevis pNU212 expression system, correlations of serological immune response to anthrax protection efficacy were analyzed in a guinea pig model.
Methods
Serological responses of rPA anthrax vaccine were investigated in guinea pigs that were given single or two injections (interval of 4 weeks) of various amounts of rPA combined with aluminumhydroxide adjuvant. Guinea pigs were subsequently challenged by the intramuscular injection with 30 half-lethal doses (30LD50) of virulent Bacillus anthracis spores. Serumantibody titerswere determined by anti-PA IgGELISA and the ability of antibodies to neutralize the cytotoxicity of lethal toxin on J774A.1 cell was measured through the toxin neutralizing antibody (TNA) assay.
Results
To examine correlations between survival rate and antibody titers, correlation between neutralizing antibody titers and the extent of protection was determined. Toxin neutralization titers of at least 1176 were sufficient to confer protection against a dose of 30LD50 of virulent anthrax spores of the H9401 strain. Such consistency in the correlation was not observed from those antibody titers determined by ELISA.
Conclusion
Neutralizing-antibody titers can be used as a surrogate marker.

Citations

Citations to this article as recorded by  
  • A putative exosporium lipoprotein GBAA0190 of Bacillus anthracis as a potential anthrax vaccine candidate
    Jun Ho Jeon, Yeon Hee Kim, Kyung Ae Kim, Yu-Ri Kim, Sun-Je Woo, Ye Jin Choi, Gi-eun Rhie
    BMC Immunology.2021;[Epub]     CrossRef
  • Construction of a bivalent vaccine against anthrax and smallpox using the attenuated vaccinia virus KVAC103
    Deok Bum Park, Bo-Eun Ahn, Hosun Son, Young-Ran Lee, Yu-Ri Kim, Su Kyoung Jo, Jeong-Hoon Chun, Jae-Yon Yu, Myung-Min Choi, Gi-eun Rhie
    BMC Microbiology.2021;[Epub]     CrossRef
  • Single-dose combination nanovaccine induces both rapid and durable humoral immunity and toxin neutralizing antibody responses against Bacillus anthracis
    Sean M. Kelly, Kristina R. Larsen, Ross Darling, Andrew C. Petersen, Bryan H. Bellaire, Michael J. Wannemuehler, Balaji Narasimhan
    Vaccine.2021; 39(29): 3862.     CrossRef
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    Vladimir Savransky, Boris Ionin, Joshua Reece
    Pathogens.2020; 9(5): 370.     CrossRef
  • Anthrax prevention through vaccine and post-exposure therapy
    Manish Manish, Shashikala Verma, Divya Kandari, Parul Kulshreshtha, Samer Singh, Rakesh Bhatnagar
    Expert Opinion on Biological Therapy.2020; 20(12): 1405.     CrossRef
  • A therapeutic human antibody against the domain 4 of the Bacillus anthracis protective antigen shows protective efficacy in a mouse model
    Bo-Eun Ahn, Hee-Won Bae, Hae-Ri Lee, Sun-Je Woo, Ok-Kyu Park, Jun Ho Jeon, Jungchan Park, Gi-eun Rhie
    Biochemical and Biophysical Research Communication.2019; 509(2): 611.     CrossRef
  • Vaccines against anthrax based on recombinant protective antigen: problems and solutions
    Olga A. Kondakova, Nikolai A. Nikitin, Ekaterina A. Evtushenko, Ekaterina M. Ryabchevskaya, Joseph G. Atabekov, Olga V. Karpova
    Expert Review of Vaccines.2019; 18(8): 813.     CrossRef
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    Theodor Chitlaru, Ma'ayan Israeli, Shahar Rotem, Uri Elia, Erez Bar-Haim, Sharon Ehrlich, Ofer Cohen, Avigdor Shafferman
    Vaccine.2017; 35(44): 6030.     CrossRef
  • Expression and refolding of the protective antigen of Bacillus anthracis: A model for high-throughput screening of antigenic recombinant protein refolding
    María Elisa Pavan, Esteban Enrique Pavan, Fabián Martín Cairó, María Julia Pettinari
    Revista Argentina de Microbiología.2016; 48(1): 5.     CrossRef
  • Protein- and DNA-based anthrax toxin vaccines confer protection in guinea pigs against inhalational challenge withBacillus cereusG9241
    John Palmer, Matt Bell, Christian Darko, Roy Barnewall, Andrea Keane-Myers
    Pathogens and Disease.2014; : n/a.     CrossRef
Human Anthrax Vaccine Development in Korea National Institute of Health
Gi-eun Rhie
Osong Public Health Res Perspect. 2011;2(Suppl 1):S9-S9.   Published online December 31, 2011
DOI: https://doi.org/10.1016/j.phrp.2011.11.032
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PHRP : Osong Public Health and Research Perspectives