Osong Public Health and Research Perspectives

Farah Sabouni

1 Article

Virulence Factors of Staphylococcus aureus Isolates in an Iranian Referral Children's Hospital: Farah Sabouni, Shima Mahmoudi, Abbas Bahador, Babak Pourakbari, Reihaneh Hosseinpour Sadeghi, Mohammad Taghi Haghi Ashtiani, Bahram Nikmanesh, Setareh Mamishi; Osong Public Health Res Perspect. 2014;5(2):96-100. Published online April 30, 2014; DOI: https://doi.org/10.1016/j.phrp.2014.03.002

2,536 View
20 Download
29 Citations

Objectives
The clinical importance of Staphylococcus aureus (S. aureus) is attributed to notable virulence factors, surface proteins, toxins, and enzymes as well as the rapid development of drug resistance. The aim of this study was to compare the occurrence of virulence factors produced by S. aureus strains isolated from children in an Iranian referral children's hospital.
Methods
The presence of genes encoding for the enterotoxins A (sea), B (seb), C (sec), D (sed), TSST-1 (tsst), exfoliative toxin A (eta), and exfoliative toxin B (etb) were detected by Multiplex polymerase chain reaction (PCR) using specific primers. In addition, the standardized Kirby-Bauer disc-diffusion method was performed on Mueller-Hinton agar.
Results
In total, 133 S. aureus isolates were obtained from different patients. Of these S. aureus isolates, 64 (48%) were methicillin-resistant S. aureus (MRSA), and all of these tested positive for the mecA gene. Regarding the classical enterotoxin genes, sea gene (40.6%) was the most prevalent followed by seb (19.6%), tsst (12.8%), eta (11.3%), etb (9%), sed (4.5%), and sec (3%). Among methicillin-susceptible S. aureus (MSSA) isolates, seb and tsst were the more prevalent toxins in comparison with MRSA isolates (p < 0.05), while the frequency of sea, sed, eta, and etb genes were higher among MRSA isolates (p > 0.05).
Conclusion
In our study enterotoxin A was produced by 40.6% of the isolates (48% from MRSA and 33% from MSSA isolates) which was higher than in previous reports. According to our results, strict hygiene and preventative measures during food processing are highly recommended.

Citations

Citations to this article as recorded by

New update on molecular diversity of clinical Staphylococcus aureus isolates in Iran: antimicrobial resistance, adhesion and virulence factors, biofilm formation and SCCmec typing
Mahtab Tabandeh, Hami Kaboosi, Mojtaba Taghizadeh Armaki, Abazar Pournajaf, Fatemeh Peyravii Ghadikolaii
Molecular Biology Reports.2022; 49(4): 3099. CrossRef
Investigation of Virulence Genes of the Predominant Bacteria Associated with Renal Stones and their Correlation with Postoperative Septic Complications
Asmaa E Ahmed, Hassan Abol-Enein, Amira Awadalla, Heba El degla, Omar A El-Shehaby
Infection and Drug Resistance.2022; Volume 15: 3643. CrossRef
Virulence genes distributed among Staphylococcus aureus causing wound infections and their correlation to antibiotic resistance
Asia Helmi Rasmi, Eman Farouk Ahmed, Abdou Mohammed Abdullah Darwish, Gamal Fadl Mahmoud Gad
BMC Infectious Diseases.2022;[Epub] CrossRef
Genetic Diversity and Virulence Profile of Methicillin and Inducible Clindamycin-Resistant Staphylococcus aureus Isolates in Western Algeria
Zahoua Mentfakh Laceb, Seydina M. Diene, Rym Lalaoui, Mabrouk Kihal, Fella Hamaidi Chergui, Jean-Marc Rolain, Linda Hadjadj
Antibiotics.2022; 11(7): 971. CrossRef
Antimicrobial susceptibility and genotyping of microorganisms isolated from sputum culture of children with cystic fibrosis in an Iranian referral children’s hospital
Setareh Mamishi, Arezu Akhlaghi, Babak Pourakbari, Mohammadreza Modaresi, Mohammad Taghi Haghi Ashtiani, Reihaneh Hosseinpour Sadeghi, Rohola Shirzadi, Zohreh Shalchi, Shima Mahmoudi
Wiener Medizinische Wochenschrift.2022;[Epub] CrossRef
Phenotypic and genotypic virulence features of staphylococcal strains isolated from difficult-to-treat skin and soft tissue infections
Mădălina Preda, Mara Mădălina Mihai, Laura Ioana Popa, Lia-Mara Dițu, Alina Maria Holban, Loredana Sabina Cornelia Manolescu, Gabriela-Loredana Popa, Andrei-Alexandru Muntean, Irina Gheorghe, Carmen Mariana Chifiriuc, Mircea-Ioan Popa, Keun Seok Seo
PLOS ONE.2021; 16(2): e0246478. CrossRef
Distribution of virulence genes and SCCmec types among methicillin-resistant Staphylococcus aureus of clinical and environmental origin: a study from community of Assam, India
Deepshikha Bhowmik, Shiela Chetri, Bhaskar Jyoti Das, Debadatta Dhar Chanda, Amitabha Bhattacharjee
BMC Research Notes.2021;[Epub] CrossRef
The anti-biofilm capability of nano-emodin-mediated sonodynamic therapy on multi-species biofilms produced by burn wound bacterial strains
Maryam Pourhajibagher, Bahman Rahimi-esboei, Hanie Ahmadi, Abbas Bahador
Photodiagnosis and Photodynamic Therapy.2021; 34: 102288. CrossRef
Prevalence of methicillin-resistant Staphylococcus aureus in Iranian children: a systematic review and meta-analysis
Farhad Sarrafzadeh, Seyed Mojtaba Sohrevardi, Hamid Abousaidi, Hossein Mirzaei
Clinical and Experimental Pediatrics.2021; 64(8): 415. CrossRef
Interactions between staphylococcal enterotoxins A and D and superantigen-like proteins 1 and 5 for predicting methicillin and multidrug resistance profiles among Staphylococcus aureus ocular isolates
Min Lu, Jean-Marie Parel, Darlene Miller, Abdelazeem Mohamed Algammal
PLOS ONE.2021; 16(7): e0254519. CrossRef
Four Weeks Exercise in Vary Intensities Reduce More Inguinal Fat Than Perigonadal Fat in Mice
Ahmad Rukhani Lutfi, Paulus Liben, Lilik Herawati
Folia Medica Indonesiana.2021; 57(2): 121. CrossRef
Molecular Genotyping of Tsst-1 Gene Staphylococcus Aureus from Clinical Specimen
Marinda Dwi Puspitarini, Kuntaman Kuntaman, Eddy Bagus Wasito, Wahyu Setyarini
Folia Medica Indonesiana.2021; 57(2): 129. CrossRef
Prevalence of enterotoxin genes (SEA to SEE) and antibacterial resistant pattern of Staphylococcus aureus isolated from clinical specimens in Assiut city of Egypt
Ahmed A. Baz, Elsayed K. Bakhiet, Usama Abdul-Raouf, Ahmed Abdelkhalek
Egyptian Journal of Medical Human Genetics.2021;[Epub] CrossRef
Contemporary systematic review and meta-analysis of exfoliative toxin-producing Staphylococcus aureus strains isolated from patients in Iran
Mojtaba Memariani, Hamed Memariani, Mohammad Shahidi-Dadras, Zohreh Tehranchinia, Fariba Ghalamkarpour, Hamideh Moravvej
Reviews in Medical Microbiology.2020; 31(1): 1. CrossRef
Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells
Inés Reigada, Ramón Pérez-Tanoira, Jayendra Z. Patel, Kirsi Savijoki, Jari Yli-Kauhaluoma, Teemu J. Kinnari, Adyary Fallarero
Microorganisms.2020; 8(3): 345. CrossRef
Characterization of different virulent factors in methicillin-resistant Staphylococcus aureus isolates recovered from Iraqis and Syrian refugees in Duhok city, Iraq
Narin A. Rasheed, Nawfal R. Hussein, Herminia de Lencastre
PLOS ONE.2020; 15(8): e0237714. CrossRef
Inhibition of growth and gene expression in Staphylococcus aureus by anti-gyrA peptide nucleic acid
Mahnaz Tavakoli, Ali Hashemi, Maryam Vaezjalali, Mohammad Mohammadzadeh, Hossein Goudarzi
Future Microbiology.2019; 14(13): 1123. CrossRef
Quorum Sensing: A Prospective Therapeutic Target for Bacterial Diseases
Qian Jiang, Jiashun Chen, Chengbo Yang, Yulong Yin, Kang Yao
BioMed Research International.2019; 2019: 1. CrossRef
Survey of Staphylococcus aureus in a general pediatric population and focus on isolates with three clinically relevant toxin-encoding genes
Anne Filleron, Sarah Beauregard-Birba, Thibault Mura, Fabien Aujoulat, Anne Laure Michon, Michel Rodière, Tu Anh Tran, Eric Jeziorski, Hélène Marchandin
World Journal of Pediatrics.2018; 14(1): 35. CrossRef
Characterization of Virulence Factors and Prophage Profiles of Methicillin-Resistant Staphylococcus aureus Strains Isolated from a Referral Hospital in Tehran, Iran
Fateh Rahimi, Leili Shokoohizadeh
Archives of Clinical Infectious Diseases.2018;[Epub] CrossRef
Inhibitory Effects of Curcumin on the Expression of NorA Efflux Pump and Reduce Antibiotic Resistance in Staphylococcus aureus
Samin Jaberi, Fateme Fallah, Ali Hashemi, Ahmad Karimi, Leila Azimi
Journal of Pure and Applied Microbiology.2018; 12(1): 95. CrossRef
Relationships among superantigen toxin gene profiles, genotypes, and pathogenic characteristics of Staphylococcus aureus isolates from bovine mastitis
Dong Wang, Limei Zhang, Changfu Yong, Mingliang Shen, Tariq Ali, Muhammad Shahid, Kun Han, Xuezhang Zhou, Bo Han
Journal of Dairy Science.2017; 100(6): 4276. CrossRef
Molecular epidemiology of Staphylococcus aureus nasal colonization among patients and their parents /guardian in an Iranian referral hospital
Babak Pourakbari, Mahmoud Khodabandeh, Shima Mahmoudi, Farah Sabouni, Alireza Aziz-Ahari, Abbas Bahador, Sepideh Keshavarz Valian, Reihaneh Hosseinpour Sadeghi, Setareh Mamishi
Microbial Pathogenesis.2017; 107: 75. CrossRef
The effect of antimicrobial photodynamic therapy on the expression of novel methicillin resistance markers determined using cDNA-AFLP approach in Staphylococcus aureus
Mohammad Neshvan Hoorijani, Hosein Rostami, Maryam Pourhajibagher, Nasim Chiniforush, Mansour Heidari, Babak Pourakbari, Hossein Kazemian, Kambiz Davari, Vahid Amini, Reza Raoofian, Abbas Bahador
Photodiagnosis and Photodynamic Therapy.2017; 19: 249. CrossRef
Antimicrobial susceptibility of microorganisms isolated from sputum culture of patients with cystic fibrosis: Methicillin-resistant Staphylococcus aureus as a serious concern
Narges Mazloomi Nobandegani, Shima Mahmoudi, Babak Pourakbari, Reihaneh Hosseinpour Sadeghi, Mehri Najafi Sani, Fateme Farahmand, Farzaneh Motamed, Raheleh Nabavizadeh Rafsanjani, Setareh Mamishi
Microbial Pathogenesis.2016; 100: 201. CrossRef
The Correlation between Toxin Genotype and Antibiotic Resistance in Methicillin ResistantStaphylococcus aureusIsolated from Clinical Specimen of Intensive Care Unit
Chul Park, Chi Nam Seong
Korean Journal of Clinical Laboratory Science.2016; 48(3): 202. CrossRef
Studies on Enterotoxins and Antimicrobial Resistance in <i>Staphylococcus aureus</i> Isolated from Various Sources
Enas Yasser Mohammed, Shaymaa Hassan Abdel-Rhman, Rasha Barwa, Mohammed Adel El-Sokkary
Advances in Microbiology.2016; 06(04): 263. CrossRef
Genotype Analyses of Methicillin Resistant Staphylococcus aureus Isolated from clinical specimens
Jean-Soo Kim, Chul Park
Journal of the Korea Academia-Industrial cooperati.2015; 16(5): 3315. CrossRef
Frequency of the toxic shock syndrome toxin-1 gene in methicillin-susceptible and -resistant Staphylococcus aureus isolates from teaching hospitals in Shiraz, Iran
Mohammad Motamedifar, Hadi Sedigh Ebrahim-Saraie, Seyedeh Mahsan Hoseini Alfatemi, Mehrdad Zalipour, Mohammad Kaveh, Hossein Khoshkharam-Roodmajani
Revista da Sociedade Brasileira de Medicina Tropic.2015; 48(1): 90. CrossRef

Author index