This study aimed to determine the presence of SARS-CoV-2 on surfaces frequently touched by COVID-19 patients, and assess the scope of contamination and transmissibility in facilities where the outbreaks occurred. In the course of this epidemiological investigation, a total of 80 environmental specimens were collected from 6 hospitals (68 specimens) and 2 “mass facilities” (6 specimens from a rehabilitation center and 6 specimens from an apartment building complex). Specific reverse transcriptase-polymerase chain reaction targeting of RNA-dependent RNA polymerase, and envelope genes, were used to identify the presence of this novel coronavirus. The 68 specimens from 6 hospitals (A, B, C, D, E, and G), where prior disinfection/cleaning had been performed before environmental sampling, tested negative for SARS-CoV-2. However, 2 out of 12 specimens (16.7%) from 2 “mass facilities” (F and H), where prior disinfection/cleaning had not taken place, were positive for SARS-CoV-2 RNA polymerase, and envelope genes. These results suggest that prompt disinfection and cleaning of potentially contaminated surfaces is an effective infection control measure. By inactivating SARS-CoV-2 with disinfection/cleaning the infectivity and transmission of the virus is blocked. This investigation of environmental sampling may help in the understanding of risk assessment of the COVID-19 outbreak in “mass facilities” and provide guidance in using effective disinfectants on contaminated surfaces.

Citations

Citations to this article as recorded by  

• Coronavirus disease 2019 (COVID-19) research agenda for healthcare epidemiology
  Lona Mody, Ibukunoluwa C. Akinboyo, Hilary M. Babcock, Werner E. Bischoff, Vincent Chi-Chung Cheng, Kathleen Chiotos, Kimberly C. Claeys, K. C. Coffey, Daniel J. Diekema, Curtis J. Donskey, Katherine D. Ellingson, Heather M. Gilmartin, Shruti K. Gohil, An
  Infection Control & Hospital Epidemiology.2022; 43(2): 156.     CrossRef
• SARS-CoV-2 on Surfaces and HVAC Filters in Dormitory Rooms
  Jin Pan, Seth A. Hawks, Aaron J. Prussin, Nisha K. Duggal, Linsey C. Marr
  Environmental Science & Technology Letters.2022; 9(1): 71.     CrossRef
• COVID-19 Cluster Linked to Aerosol Transmission of SARS-CoV-2 via Floor Drains
  Taewon Han, Heedo Park, Yungje Jeong, Jungmin Lee, Eungyeong Shon, Man-Seong Park, Minki Sung
  The Journal of Infectious Diseases.2022; 225(9): 1554.     CrossRef
• Environmental Contamination with SARS-CoV-2 in Hospital COVID Department: Antigen Test, Real-Time RT-PCR and Virus Isolation
  Urška Rozman, Lea Knez, Goran Novak, Jernej Golob, Anita Pulko, Mojca Cimerman, Matjaž Ocepek, Urška Kuhar, Sonja Šostar Turk
  COVID.2022; 2(8): 1050.     CrossRef
• Using Environmental Sampling to Enable Zoonotic Pandemic Preparedness
  Avirup Sanyal, Sanskriti Agarwal, Uma Ramakrishnan, Kritika M. Garg, Balaji Chattopadhyay
  Journal of the Indian Institute of Science.2022; 102(2): 711.     CrossRef
• Anforderungen an die Hygiene bei der Reinigung und Desinfektion von Flächen
  
  Bundesgesundheitsblatt - Gesundheitsforschung - Ge.2022; 65(10): 1074.     CrossRef
• SARS-CoV-2 pandemic: a review of molecular diagnostic tools including sample collection and commercial response with associated advantages and limitations
  Harikrishnan Jayamohan, Christopher J. Lambert, Himanshu J. Sant, Alexander Jafek, Dhruv Patel, Haidong Feng, Michael Beeman, Tawsif Mahmood, Ugochukwu Nze, Bruce K. Gale
  Analytical and Bioanalytical Chemistry.2021; 413(1): 49.     CrossRef
• Contamination of inert surfaces by SARS-CoV-2: Persistence, stability and infectivity. A review
  Montse Marquès, José L. Domingo
  Environmental Research.2021; 193: 110559.     CrossRef
• A Systematic Review of Surface Contamination, Stability, and Disinfection Data on SARS-CoV-2 (Through July 10, 2020)
  Noah Bedrosian, Elizabeth Mitchell, Elsa Rohm, Miguel Rothe, Christine Kelly, Gabrielle String, Daniele Lantagne
  Environmental Science & Technology.2021; 55(7): 4162.     CrossRef
• Transmission of Severe Acute Respiratory Syndrome Coronavirus 2 via Contaminated Surfaces: What Is to Be Done?
  Craig S Conover
  Clinical Infectious Diseases.2021; 72(11): 2062.     CrossRef
• Investigation of SARS CoV-2 virus in environmental surface
  Abdollah Dargahi, Farhad Jeddi, Mehdi Vosoughi, Chiman Karami, Aidin Hadisi, S. Ahamad Mokhtari, Hasan Ghobadi, Morteza Alighadri, Somayeh Biparva Haghighi, Hadi Sadeghi
  Environmental Research.2021; 195: 110765.     CrossRef
• Ist die Desinfektion öffentlicher Flächen zur Prävention von SARS-CoV-2 – infektionen sinnvoll?
  Günter Kampf, Lutz Jatzwauk
  Das Gesundheitswesen.2021; 83(03): 180.     CrossRef
• SARS-CoV-2 Detection Rates from Surface Samples Do Not Implicate Public Surfaces as Relevant Sources for Transmission
  Günter Kampf, Stephanie Pfaender, Emanuel Goldman, Eike Steinmann
  Hygiene.2021; 1(1): 24.     CrossRef
• The SARS-CoV-2 (COVID-19) pandemic in hospital: An insight into environmental surfaces contamination, disinfectants’ efficiency, and estimation of plastic waste production
  Faezeh seif, Zahra Noorimotlagh, Seyyed Abbas Mirzaee, Mojtaba Kalantar, Barat Barati, Mahdi Emamian Fard, Nozar Kalantar Fard
  Environmental Research.2021; 202: 111809.     CrossRef
• Prevalence of SARS-CoV-2 RNA on inanimate surfaces: a systematic review and meta-analysis
  Simone Belluco, Marzia Mancin, Filippo Marzoli, Alessio Bortolami, Eva Mazzetto, Alessandra Pezzuto, Michela Favretti, Calogero Terregino, Francesco Bonfante, Roberto Piro
  European Journal of Epidemiology.2021; 36(7): 685.     CrossRef
• Management following the first confirmed case of SARS-CoV-2 in a domestic cat associated with a massive outbreak in South Korea
  Taewon Han, Boyeong Ryu, Suyeon Lee, Yugyeong Song, Yoongje Jeong, Ilhwan Kim, Jeongmin Kim, Eunjin Kim, Wonjun Lee, Hyunju Lee, Haekyoung Hwang
  One Health.2021; 13: 100328.     CrossRef
• Non-Respiratory Droplet Transmission of COVID-19 in the Isolation Ward of a Secondary Hospital in Oman
  Zayid K. Al Mayahi, Nawal Al Kindi, Nasser Al Shaqsi, Noaman Al Hattali, Azza Al Hattali, Khalid Salim, Mark Beatty
  Infectious Diseases in Clinical Practice.2021; 29(6): e371.     CrossRef
• Effectiveness of antiviral metal and metal oxide thin-film coatings against human coronavirus 229E
  Louis-Vincent Delumeau, Hatameh Asgarimoghaddam, Tamiru Alkie, Alexander James Bryan Jones, Samantha Lum, Kissan Mistry, Marc G. Aucoin, Stephanie DeWitte-Orr, Kevin P. Musselman
  APL Materials.2021; 9(11): 111114.     CrossRef
• Rapid Review of SARS-CoV-1 and SARS-CoV-2 Viability, Susceptibility to Treatment, and the Disinfection and Reuse of PPE, Particularly Filtering Facepiece Respirators
  José G. B. Derraik, William A. Anderson, Elizabeth A. Connelly, Yvonne C. Anderson
  International Journal of Environmental Research an.2020; 17(17): 6117.     CrossRef
• Potential sources, modes of transmission and effectiveness of prevention measures against SARS-CoV-2
  G. Kampf, Y. Brüggemann, H.E.J. Kaba, J. Steinmann, S. Pfaender, S. Scheithauer, E. Steinmann
  Journal of Hospital Infection.2020; 106(4): 678.     CrossRef