<b>Objectives</b><br/>
<i>Plasmodium vivax</i> merozoite surface protein 1 (PvMSP1) is the most intensively studied malaria vaccine candidate. Although high antibody response-inducing two C-terminal fragments of PvMSP1 (PvMSP1-19 and PvMSP1-42) are currently being developed as candidate malaria vaccine antigens, their high genetic diversity in various isolates is a major hurdle. The sequence polymorphism of PvMSP1 has been investigated; however, the humoral immune responses induced by different portions of this protein have not been evaluated in Korea.<br/><b>Methods</b><br/>
Two fragments of PvMSP1 were selected for this study: (1) PvMSP1-19, which is genetically conserved; and (2) PvMSP1-33, which corresponds to a variable portion. For the latter, two representative strains, Sal 1 and Belem, were included. Thus, three recombinant proteins, PvMSP1-19, PvMSP1-33 Sal 1, and PvMSP1-33 Belem, were produced in <i>Escherichia coli</i> and then tested by enzyme-linked immunosorbent assays using sera from 221 patients with vivax malaria.<br/><b>Results</b><br/>
Of the 221 samples, 198, 142, and 106 samples were seropositive for PvMSP1-19, PvMSP1-33 Sal 1, and PvMSP1-33 Belem, respectively. Although 100 samples were simultaneously seropositive for antibodies specific to all the recombinant proteins, 39 and six samples were respectively seropositive for antibodies specific to MSP1-33 Sal 1 and MSP1-33 Belem. Antibodies specific to PvMSP1-19 were the most prevalent.<br/><b>Conclusion</b><br/>
Monitoring seroprevalence is essential for the selection of promising vaccine candidates as most of the antigenic proteins in <i>P. vivax</i> are highly polymorphic.
Citations
Citations to this article as recorded by
Plasmodium vivax MSP1-42 kD Variant Proteins Detected Naturally Induced IgG Antibodies in Patients Regardless of the Infecting Parasite Phenotype in Mesoamerica Lilia Gonzalez-Ceron, Barbara Dema, Olga L. Palomeque-Culebro, Frida Santillan-Valenzuela, Alberto Montoya, Arturo Reyes-Sandoval Life.2023; 13(3): 704. CrossRef
Spatiotemporal Changes in Plasmodium vivax msp142 Haplotypes in Southern Mexico: From the Control to the Pre-Elimination Phase Alejandro Flores-Alanis, Lilia González-Cerón, Frida Santillán-Valenzuela, Cecilia Ximenez, Marco A. Sandoval-Bautista, Rene Cerritos Microorganisms.2022; 10(1): 186. CrossRef
Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates Xiang Ting Goh, Yvonne A.L. Lim, Ping Chin Lee, Veeranoot Nissapatorn, Kek Heng Chua Molecular and Biochemical Parasitology.2021; 244: 111390. CrossRef
Humoral and cellular immune response to Plasmodium vivax VIR recombinant and synthetic antigens in individuals naturally exposed to P. vivax in the Republic of Korea Sanghyun Lee, Young-Ki Choi, Youn-Kyoung Goo Malaria Journal.2021;[Epub] CrossRef