- A Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis
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Saeed Alamian, Majid Esmaelizad, Taghi Zahraei, Afshar Etemadi, Mohsen Mohammadi, Davoud Afshar, Soheila Ghaderi
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Osong Public Health Res Perspect. 2017;8(1):65-70. Published online February 28, 2017
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DOI: https://doi.org/10.24171/j.phrp.2017.8.1.09
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Abstract
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- Objectives
Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis. MethodsAll complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available Brucella sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate Brucella abortus from Brucella melitensis. A primer set was designed to flank the unique locus. A total of 136 lymph nodes and blood samples were evaluated and classified by the URS-polymerase chain reaction (PCR) method in 2013–2014. ResultsBiochemical tests and bacteriophage typing as the golden standard indicated that all Brucella spp. isolates were B. melitensis biovar 1 and B. abortus biovar 3. The PCR results were the same as the bacteriological method for detecting Brucella spp. The sensitivity and specificity of the URS-PCR method make it suitable for detecting B. abortus and B. melitensis. ConclusionQuick detection of B. abortus and B. melitensis can provide the most effective strategies for control of these bacteria. The advantage of this method over other presented methods is that both B. abortus and B. melitensis are detectable in a single test tube. Furthermore, this method covered 100% of all B. melitensis and B. abortus biotypes. The development of this URS-PCR method is the first step toward the development of a novel kit for the molecular identification of B. abortus and B. melitensis.
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Citations
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- Investigating the Rate of Contamination of Milk and Traditional Dairy Products with Brucella Bacteria by PCR Method, Mamasani District, Fars Province, South of Iran
M Kalantari, N Askarpour, K Azizi, M Yousefi, M Amin, M Vahedi, A Keshavarz Armaghane Danesh.2024; 29(2): 231. CrossRef - Development of a simplified and cost-effective sample preparation method for genotyping of human papillomavirus by next-generation sequencing
Rungrat Jitvaropas, Ukrit Thongpoom, Vorthon Sawaswong, Kritsada Khongnomnan, Witthaya Poomipak, Kesmanee Praianantathavorn, Pornjarim Nilyanimit, Yong Poovorawan, Sunchai Payungporn Archives of Virology.2023;[Epub] CrossRef - Bovine brucellosis – a comprehensive review
Sandip Kumar Khurana, Anju Sehrawat, Ruchi Tiwari, Minakshi Prasad, Baldev Gulati, Muhammad Zubair Shabbir, Rajesh Chhabra, Kumaragurubaran Karthik, Shailesh Kumar Patel, Mamta Pathak, Mohd. Iqbal Yatoo, Vivek Kumar Gupta, Kuldeep Dhama, Ranjit Sah, Wanpe Veterinary Quarterly.2021; 41(1): 61. CrossRef - Survey of Zoonotic Bacterial Pathogens in Native Foxes in Central Chile: First Record of Brucella canis Exposure
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Richa Hans, Pranjal Kumar Yadav, Pushpendra Kumar Sharma, Mannan Boopathi, Duraipandian Thavaselvam Scientific Reports.2020;[Epub] CrossRef - Laboratory Diagnostic Procedures for Human Brucellosis: An Overview of Existing Approaches
Afshar Etemadi, Rezvan Moniri, Heinrich Neubauer, Yasaman Dasteh Goli, Saeed Alamian Jundishapur Journal of Microbiology.2019;[Epub] CrossRef - Comparison of PCR-RFLP and PFGE for determining the clonality of Brucella isolates from human and livestock specimens
Nasrin Bahmani, Reza Mirnejad, Mohammad Reza Arabestani, Parviz Mohajerie, Seyed Hamid Hashemi, Manoochehr Karami, Mohammad Yousef Alikhani Saudi Journal of Biological Sciences.2019; 26(2): 256. CrossRef -
Designing an immunosensor for detection of
Brucella abortus
based on coloured silica nanoparticles
Arash Shams, Bahareh Rahimian Zarif, Mojtaba Salouti, Reza Shapouri, Sako Mirzaii Artificial Cells, Nanomedicine, and Biotechnology.2019; 47(1): 2562. CrossRef - Identification of Brucella genus and eight Brucella species by Luminex bead-based suspension array
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- Rapid Molecular Approach for Simultaneous Detection of Salmonella spp., Shigella spp., and Vibrio cholera
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Reza Ranjbar, Ali Naghoni, Davoud Afshar, Farhad Nikkhahi, Mohsen Mohammadi
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Osong Public Health Res Perspect. 2016;7(6):373-377. Published online December 31, 2016
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DOI: https://doi.org/10.1016/j.phrp.2016.10.002
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3,830
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26
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Abstract
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- Objectives
Gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including Iran. Early detection of the gastrointestinal tract pathogens can be extremely important. The aim of the current study was to apply a shortened time-multiplex polymerase chain reaction (PCR) for rapid and simultaneous detection of Salmonella spp., Shigella spp., and Vibrio cholera. Methods
The standard and clinical strains of Salmonella spp., Shigella spp., and V. cholerae were used in the assay. Multiplex PCR was performed and optimized based on amplification of invA, putative integrase, and ompW genes for detecting Salmonella spp., Shigella spp., and V. cholerae, respectively. The specificity of the assay was evaluated by testing 12 different bacterial species. Results
Only Salmonella spp., Shigella spp., and V. cholerae strains had positive results when subjected to the assay using multiplex PCR. The assay showed a high sensitivity, and no amplification products were observed in multiplex PCR with any of the other microorganisms. Conclusion
Our study indicated that the invA, putative integrase, and ompW-based multiplex PCR assay appears to be an efficient method for rapid and simultaneous detection of Salmonella spp., Shigella spp., and V. cholerae.
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Wei Guo, Qian Yang, Jie Liu, Xiuling Chen, Yunzhe Zhang, Wei Zhang LWT.2022; 168: 113875. CrossRef - Campylobacter Species in the Middle East
Daryoush Babazadeh, Reza Ranjbar Journal of Veterinary Physiology and Pathology.2022; 1(1): 1. CrossRef - Development of rapid gold nanoparticles based lateral flow assays for simultaneous detection of Shigella and Salmonella genera
Mohammad Lukman Yahaya, Nor Dyana Zakaria, Rahmah Noordin, Khairunisak Abdul Razak Biotechnology and Applied Biochemistry.2021; 68(5): 1095. CrossRef - Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor
Cheryl S.Y. Yeap, Thanyarat Chaibun, Su Yin Lee, Bin Zhao, Yuan Jan, Chan La-o-vorakiat, Werasak Surareungchai, Shiping Song, Benchaporn Lertanantawong Chemical Communications.2021; 57(91): 12155. CrossRef - Taqman hydrolysis probe application for Escherichia coli, Salmonella enterica, and Vibrio cholerae detection in surface and drinking water
Ahmed K. A. El-Sayed, Mohamed I. Abou-Dobara, Camelia A. Abdel-Malak, Amira A. E. El-Badaly Journal of Water, Sanitation and Hygiene for Devel.2019; 9(3): 492. CrossRef - Pathways of healthcare and antibiotics use following reported gastrointestinal illness: a cross-sectional study in rural Anhui, China
Xing Rong Shen, Maomao Xie, Jing Chai, Rui Feng, Jing Cheng, Rong Liu, Paul Kadetz, DeBin Wang BMJ Open.2019; 9(8): e030986. CrossRef - DNA Microarray for Rapid Detection and Identification of Food and Water Borne Bacteria: From Dry to Wet Lab
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