- Original Article
- Development of a Diagnostic Kit to Detect Cryptosporidium parvum and Giardia lamblia
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Hyeng-Il Cheun, Byung-Suk Chung, Da-Won Ma, Bo-La Goo, Shin-Hyeong Cho, Mi-jung Ji, Won-Ja Lee
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Osong Public Health Res Perspect. 2013;4(3):146-151. Published online June 30, 2013
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Objectives
This study aims to develop a high-sensitivity antibody diagnostic kit that will enable a rapid and accurate detection of Cryptospofidium parvum and Giardia lamblia in patients with diarrhea.
Methods
The cultivated C. parvum oocysts and G. lamblia cysts in each calf and dog were injected to mice to obtain antibodies, which were titrated. Spleen cells of the immunized mouse were separated and... |
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- The First Outbreak of Giardiasis with Drinking Water in Korea
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Hyeng-Il Cheun, Cheon-Hyeon Kim, Shin-Hyeong Cho, Da-Won Ma, Bo-La Goo, Mun-Su Na, Seung-Ki Youn, Won-Ja Lee
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Osong Public Health Res Perspect. 2013;4(2):89-92. Published online April 30, 2013
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Objectives:
To identify the pathogen of the diarrhea outbreak in a village in Jeollabuk province in Korea in April 2010.
Methods:
DNA extraction was performed from the 120 L of collected water, which was centrifuged at 10,000 x g for 30 min. PCR reactions were conducted in a total of 25 ul, which included PCR premix (GenDEPOT, Barker, TX, USA), 2 ul (∼100... |
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