- Development of a Diagnostic Kit to Detect Cryptosporidium parvum and Giardia lamblia
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Hyeng-Il Cheun, Byung-Suk Chung, Da-Won Ma, Bo-La Goo, Shin-Hyeong Cho, Mi-jung Ji, Won-Ja Lee
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Osong Public Health Res Perspect. 2013;4(3):146-151. Published online June 30, 2013
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DOI: https://doi.org/10.1016/j.phrp.2013.04.006
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 Abstract
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- Objectives
This study aims to develop a high-sensitivity antibody diagnostic kit that will enable a rapid and accurate detection of Cryptospofidium parvum and Giardia lamblia in patients with diarrhea.
Methods
The cultivated C. parvum oocysts and G. lamblia cysts in each calf and dog were injected to mice to obtain antibodies, which were titrated. Spleen cells of the immunized mouse were separated and blended with myelomas to produce hybrid cell lines that form monoclonal antibodies. Using ELISA method, antibodies that specifically respond to C. parvum and G.lamblia were then selected. The cells were injected into the abdominal cavity of a BALB/c mouse to isolate hydrops abdominis containing high level of antibodies. The IgG antibody was purified using protein G gel.
Results
The detection limit of monoclonal antibodies for Cryptosporidium parvum and Giardia lamblia was 125 oocysts/mL and 1250 cysts/mL, respectively. In addition, during testing they did not show cross-reactivity to viruses (n = 15), bacteria (n =17), and parasites (n = 9).
Conclusion
The rapid diagnostic antibody kit developed in this study, which specifically responds to C. parvum and G. lamblia, will be useful in detecting and monitoring diarrheal infections.
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Citations
Citations to this article as recorded by  - Comprehensive review of conventional and state-of-the-art detection methods of Cryptosporidium
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Biotechnology Journal.2017;[Epub] CrossRef - ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults
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American Journal of Gastroenterology.2016; 111(5): 602. CrossRef - Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler’s Diarrhea
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The Korean Journal of Parasitology.2016; 54(5): 631. CrossRef - Advancing Cryptosporidium Diagnostics from Bench to Bedside
Raul V. Destura, Rohani B. Cena, Ma. Jowina H. Galarion, Coleen M. Pangilinan, Geraldine M. Arevalo, Ryan Oliver C. Alba, Joy Ann G. Petronio, Gielenny M. Salem, Brian Schwem, Jesus Emmanuel A. D. Sevilleja
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