- Development of a Specific and Rapid Diagnostic Method for Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay
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Mi Jung Ji, Byung Ki Cho, Young Shik Cho, Young Jin Choi, Donghyok Kwon, Kyeongcheol Shin, Joo-Yeon Lee, Chun Kang, Byoung Su Yoon
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Osong Public Health Res Perspect. 2013;4(6):342-346. Published online December 31, 2013
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DOI: https://doi.org/10.1016/j.phrp.2013.10.006
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 Abstract
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- Objectives
The aim of this study was to develop an immunochromatographic assay (ICA) for the detection of influenza A (H1N1) pdm09 virus infection.
Materials and methods
Several monoclonal antibodies against influenza A (H1N1) pdm09 virus were generated and an ICA (pdm09-ICA) was developed for the rapid and specific detection of influenza A (H1N1) pdm09 virus infection. The specificity and sensitivity of the developed assay were compared with that of hemagglutination assay and real-time reverse-transcription polymerase chain reaction (rRT-PCR).
Results
The detection limit was estimated to be 1/2 (8) hemagglutinating unit; the sensitivity and specificity rates of pdm09-ICA were 75.86% (110/145) and 100% (43/43), respectively, compared with rRT-PCR. The cross-reactivity for 20 influenza viruses, including seasonal H1N1 viruses, was found to be negative except for the H1N1 virus (A/Swine/Korea/GC0503/2005).
Conclusion
These results indicate that the proposed method can be easily used for rapid and specific detection of the pdm09 infection. The assay developed in this study would be a useful tool for distinguishing the pdm09 infection from seasonal influenza A and B infections.
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Citations
Citations to this article as recorded by  - Sensitive detection of influenza a virus based on a CdSe/CdS/ZnS quantum dot-linked rapid fluorescent immunochromatographic test
Anh Viet Thi Nguyen, Tung Duy Dao, Tien Thi Thuy Trinh, Du-Young Choi, Seung-Taek Yu, Hyun Park, Seon-Ju Yeo
Biosensors and Bioelectronics.2020; 155: 112090. CrossRef - Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay
Viroj Wiwanitkit
Osong Public Health and Research Perspectives.2014; 5(2): 115. CrossRef
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