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Amin Radmanesh 1 Article
Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant Staphylococcus Aureus
Ahmad Nasser, Reza Azizian, Mohsen Tabasi, Jamil Kheirvari Khezerloo, Fatemah Sadeghpour Heravi, Morovat Taheri Kalani, Norkhoda Sadeghifard, Razieh Amini, Iraj Pakzad, Amin Radmanesh, Farid Azizi Jalilian
Osong Public Health Res Perspect. 2019;10(1):20-24.   Published online February 28, 2019
DOI: https://doi.org/10.24171/j.phrp.2019.10.1.05
  • 4,563 View
  • 57 Download
  • 10 Citations
AbstractAbstract PDF
Objectives

The emergence of resistant bacteria is being increasingly reported around the world, potentially threatening millions of lives. Amongst resistant bacteria, methicillin-resistant Staphylococcus aureus (MRSA) is the most challenging to treat. This is due to emergent MRSA strains and less effective traditional antibiotic therapies to Staphylococcal infections. The use of bacteriophages (phages) against MRSA is a new, potential alternate therapy. In this study, morphology, genetic and protein structure of lytic phages against MRSA have been analysed.

Methods

Isolation of livestock and sewage bacteriophages were performed using 0.4 μm membrane filters. Plaque assays were used to determine phage quantification by double layer agar method. Pure plaques were then amplified for further characterization. Sulfate-polyacrylamide gel electrophoresis and random amplification of polymorphic DNA were run for protein evaluation, and genotyping respectively. Transmission electron microscope was also used to detect the structure and taxonomic classification of phage visually.

Results

Head and tail morphology of bacteriophages against MRSA were identified by transmission electron microscopy and assigned to the Siphoviridae family and the Caudovirales order.

Conclusion

Bacteriophages are the most abundant microorganism on Earth and coexist with the bacterial population. They can destroy bacterial cells successfully and effectively. They cannot enter mammalian cells which saves the eukaryotic cells from lytic phage activity. In conclusion, phage therapy may have many potential applications in microbiology and human medicine with no side effect on eukaryotic cells.

Citations

Citations to this article as recorded by  
  • Isolation and characterization of lytic bacteriophages from sewage at an egyptian tertiary care hospital against methicillin-resistant Staphylococcus aureus clinical isolates
    Safia Samir, Amira El-Far, Hend Okasha, Rania Mahdy, Fatima Samir, Sami Nasr
    Saudi Journal of Biological Sciences.2022; 29(5): 3097.     CrossRef
  • Staphylococcus aureus: Biofilm Formation and Strategies Against it
    Ahmad Nasser , Mohammad Mehdi Soltan Dallal, Shiva Jahanbakhshi, Taher Azimi, Leila Nikouei
    Current Pharmaceutical Biotechnology.2022; 23(5): 664.     CrossRef
  • An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization
    Israa M. Abd-Allah, Ghadir S. El-Housseiny, Mohammad Y. Alshahrani, Samar S. El-Masry, Khaled M. Aboshanab, Nadia A. Hassouna
    Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
  • Molecular mechanisms of Shigella effector proteins: a common pathogen among diarrheic pediatric population
    Ahmad Nasser, Mehrdad Mosadegh, Taher Azimi, Aref Shariati
    Molecular and Cellular Pediatrics.2022;[Epub]     CrossRef
  • A Metal-Containing NP Approach to Treat Methicillin-Resistant Staphylococcus aureus (MRSA): Prospects and Challenges
    Wendy Wai Yeng Yeo, Sathiya Maran, Amanda Shen-Yee Kong, Wan-Hee Cheng, Swee-Hua Erin Lim, Jiun-Yan Loh, Kok-Song Lai
    Materials.2022; 15(17): 5802.     CrossRef
  • Electrochemical Biosensors for Pathogen Detection: An Updated Review
    Morteza Banakar, Masoud Hamidi, Zohaib Khurshid, Muhammad Sohail Zafar, Janak Sapkota, Reza Azizian, Dinesh Rokaya
    Biosensors.2022; 12(11): 927.     CrossRef
  • Isolation of a lytic bacteriophage for Helicobacter pylori
    Sara Khosravi, Razieh Amini, Mohammad Reza Arabestani, Seyed Saman Talebi, Farid Azizi Jalilian
    Gene Reports.2021; 23: 101107.     CrossRef
  • Bacteriophage Therapy for Critical and High-Priority Antibiotic-Resistant Bacteria and Phage Cocktail-Antibiotic Formulation Perspective
    Gursneh Kaur, Ritika Agarwal, Rakesh Kumar Sharma
    Food and Environmental Virology.2021; 13(4): 433.     CrossRef
  • A comprehensive review of bacterial osteomyelitis with emphasis on Staphylococcus aureus
    Ahmad Nasser, Taher Azimi, Soheila Ostadmohammadi, Samaneh Ostadmohammadi
    Microbial Pathogenesis.2020; 148: 104431.     CrossRef
  • Characterization of the Three New Kayviruses and Their Lytic Activity Against Multidrug-Resistant Staphylococcus aureus
    Natalia Łubowska, Bartłomiej Grygorcewicz, Katarzyna Kosznik-Kwaśnicka, Agata Zauszkiewicz-Pawlak, Alicja Węgrzyn, Barbara Dołęgowska, Lidia Piechowicz
    Microorganisms.2019; 7(10): 471.     CrossRef

PHRP : Osong Public Health and Research Perspectives