- Cell Death Mechanisms in Esophageal Squamous Cell Carcinoma Induced by Vesicular Stomatitis Virus Matrix Protein
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Yousef Douzandegan, Alireza Tahamtan, Zahra Gray, Hadi Razavi Nikoo, Alijan Tabarraei, Abdolvahab Moradi
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Osong Public Health Res Perspect. 2019;10(4):246-252. Published online August 31, 2019
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DOI: https://doi.org/10.24171/j.phrp.2019.10.4.08
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 Abstract
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Objectives
Vesicular stomatitis virus (VSV) is under development as an oncolytic virus due to its preferential replication in cancer cells and oncolytic activity, however the viral components responsible have not yet been determined. In this study the effects of VSV wild-type (wt) and M51R-mutant matrix proteins (M51R-mMP) on apoptosis, pyroptosis, necroptosis, and autophagy pathways, in an esophagus cancer cell line (KYSE-30) were investigated.
Methods
The KYSE-30 cells were transfected with pcDNA3.1 plasmids encoding wt or M51R-mMP, and apoptosis, pyroptosis, necroptosis, and autophagy were evaluated 48 and 72 hours after transfection.
Results
KYSE-30 cells transfected with VSV wt and M51R-mMPs significantly reduced cell viability to < 50% at 72 hours post-transfection. M51R-MP significantly increased the concentration of caspase-8 and caspase-9 at 48 and 72 hours post-transfection, respectively ( p < 0.05). In contrast, no significant changes were detected following transfection with the VSV wt plasmid. Moreover, VSV wt and M51R-mMP transfected cells did not change the expression of caspase-3. VSV wt and M51R-mMPs did not mMP change caspase-1 expression (a marker of pyroptosis) at 48 and 72 hours post-transfection. However, M51R-mMP and VSV wt transfected cells significantly increased RIP-1 (a marker of necroptosis) expression at 72 hours post-infection ( p < 0.05). Beclin-1, a biomarker of autophagy, was also induced by transfection with VSV wt or M51R-mMPs at 48 hours post-transfection.
Conclusion
The results in this study indicated that VSV exerts oncolytic activity in KYSE-30 tumor cells through different cell death pathways, suggesting that M51R-mMP may potentially be used to enhance oncolysis.
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Citations
Citations to this article as recorded by  - Evoking pyroptosis with nanomaterials for cancer immunotherapy: Current boom and novel outlook
Wen-Da Wang, Zhi-Jun Sun
Nano TransMed.2022; 1(1): 9130001. CrossRef - Biological causes of immunogenic cancer cell death (ICD) and anti-tumor therapy; Combination of Oncolytic virus-based immunotherapy and CAR T-cell therapy for ICD induction
Amirhossein Mardi, Anastasia V. Shirokova, Rebar N. Mohammed, Ali Keshavarz, Angelina O. Zekiy, Lakshmi Thangavelu, Talar Ahmad Merza Mohamad, Faroogh Marofi, Navid Shomali, Amir Zamani, Morteza Akbari
Cancer Cell International.2022;[Epub] CrossRef - Oncolytic Viruses: Immunotherapy Drugs for Gastrointestinal Malignant Tumors
Qingbo Li, Patrick Kwabena Oduro, Rui Guo, Ruiqiao Li, Ling Leng, Xianbin Kong, Qilong Wang, Long Yang
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Live-attenuated poliovirus-induced extrinsic apoptosis through Caspase 8 within breast cancer cell lines expressing CD155
Hossein Vazeh, Emad Behboudi, Anahita Hashemzadeh-Omran, Abdolvahab Moradi
Breast Cancer.2022; 29(5): 899. CrossRef - Exogenous expression of both matrix protein and glycoprotein facilitates infectious viral particle production of Borna disease virus 1
Takehiro Kanda, Madoka Sakai, Akiko Makino, Keizo Tomonaga
Journal of General Virology
.2022;[Epub] CrossRef - La herencia de Prometeo. Las enfermedades ocupacionales en el Corpus Hippocraticum
César Sierra Martín
Asclepio.2022; 74(1): p587. CrossRef - Analyses of cell death mechanisms related to amino acid substitution at position 95 in the rabies virus matrix protein
Isshu Kojima, Fumiki Izumi, Makoto Ozawa, Yoshikazu Fujimoto, Misuzu Okajima, Naoto Ito, Makoto Sugiyama, Tatsunori Masatani
Journal of General Virology
.2021;[Epub] CrossRef - The role of non-apoptotic cell death in the treatment and drug-resistance of digestive tumors
Yang Yang, LiangLiang Bai, Weiting Liao, Mingyang Feng, Mengxi Zhang, Qiuji Wu, Kexun Zhou, Feng Wen, Wanting Lei, Nan Zhang, Jiaxing Huang, Qiu Li
Experimental Cell Research.2021; 405(2): 112678. CrossRef - NEBL and AKT1 maybe new targets to eliminate the colorectal cancer cells resistance to oncolytic effect of vesicular stomatitis virus M-protein
Zoleikha Mamizadeh, Mohamad Reza Kalani, Masoud Parsania, Mohammad Mehdi Soltan Dallal, Abdolvahab Moradi
Molecular Therapy - Oncolytics.2021; 23: 593. CrossRef
- Natural Infection with Rabies Virus: A Histopathological and Immunohistochemical Study of Human Brains
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Firouzeh Farahtaj, Leila Alizadeh, Alireza Gholami, Alireza Tahamtan, Sadegh Shirian, Maryam Fazeli, Amir Sasan Mozaffari Nejad, Ali Gorji, Hamid Mahmoudzadeh Niknam, Amir Ghaemi
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Osong Public Health Res Perspect. 2019;10(1):6-11. Published online February 28, 2019
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DOI: https://doi.org/10.24171/j.phrp.2019.10.1.03
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9,591
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288
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12
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Abstract
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Objectives
Despite all the efforts and increased knowledge of rabies, the exact mechanisms of infection and mortality from the rabies virus are not well understood. To understand the mechanisms underlying the pathogenicity of rabies virus infection, it is crucial to study the tissue that the rabies virus naturally infects in humans.
Methods
Cerebellum brain tissue from 9 human post mortem cases from Iran, who had been infected with rabies virus, were examined histopathologically and immunohistochemically to evaluate the innate immune responses against the rabies virus.
Results
Histopathological examination revealed inflammation of the infected cerebellum and immunohistochemical analyses showed an increased immunoreactivity of heat shock protein 70, interleukin-6, interleukin-1, tumor necrosis factor-alpha, caspase-3, caspase-9, toll-like receptor3 and toll-like receptor4 in the infected brain tissue.
Conclusion
These results indicated the involvement of innate immunity in rabies infected human brain tissue, which may aggravate the progression of this deadly disease.
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Citations
Citations to this article as recorded by - Molecular Mechanisms Associated with Neurodegeneration of Neurotropic Viral Infection
Prapimpun Wongchitrat, Theerawut Chanmee, Piyarat Govitrapong
Molecular Neurobiology.2024; 61(5): 2881. CrossRef - Systematic development of immunohistochemistry protocol for large cryosections-specific to non-perfused fetal brain
Karthika Pandurangan, Jaikishan Jayakumar, Stephen Savoia, Reetuparna Nanda, S. Lata, E. Harish Kumar, Suresh S., Sudha Vasudevan, Chitra Srinivasan, Jayaraj Joseph, Mohanasankar Sivaprakasam, Richa Verma
Journal of Neuroscience Methods.2024; 405: 110085. CrossRef - Biosensor as an alternative diagnostic method for rabies virus detection: A literature review
Milad Zandi, Sajad Zandi, Ramin Mohammadi, Parastoo Hosseini, Samane Teymouri, Saber Soltani, Azadeh Rasouli
Biotechnology and Applied Biochemistry.2022; 69(4): 1348. CrossRef - Immunohistochemical diagnosis of human infectious diseases: a review
Hamadou Oumarou Hama, Gérard Aboudharam, Rémi Barbieri, Hubert Lepidi, Michel Drancourt
Diagnostic Pathology.2022;[Epub] CrossRef - Mitochondrial Dysfunction in Rabies Virus-Infected Human and Canine Brains
Pulleri Kandi Harsha, Sathyanarayanan Ranganayaki, Gowri Yale, Gourav Dey, Kiran K. Mangalaparthi, Anusha Yarlagadda, B. K. Chandrasekhar Sagar, Anita Mahadevan, M. M. Srinivas Bharath, Reeta S. Mani
Neurochemical Research.2022; 47(6): 1610. CrossRef - A rare fatal case of rabies coexisting with COVID-19
Rabi Narayan Hota, Shalendra Singh, Rakesh Sharma, Pallavi Khandare
Journal of Acute Disease.2022; 11(3): 129. CrossRef - Enhancement of immune responses by co-stimulation of TLR3 - TLR7 agonists as a potential therapeutics against rabies in mouse model
Firouzeh Farahtaj, Alireza Gholami, Mohammad Sadeq Khosravy, Safoora Gharibzadeh, Hamid Mahmoudzadeh Niknam, Amir Ghaemi
Microbial Pathogenesis.2021; 157: 104971. CrossRef - Establishment of Human-Induced Pluripotent Stem Cell-Derived Neurons—A Promising In Vitro Model for a Molecular Study of Rabies Virus and Host Interaction
Thanathom Chailangkarn, Nathiphat Tanwattana, Thanakorn Jaemthaworn, Sira Sriswasdi, Nanchaya Wanasen, Sithichoke Tangphatsornruang, Kantinan Leetanasaksakul, Yuparat Jantraphakorn, Wanapinun Nawae, Penpicha Chankeeree, Porntippa Lekcharoensuk, Boonlert L
International Journal of Molecular Sciences.2021; 22(21): 11986. CrossRef - Beneficial and Detrimental Effects of Regulatory T Cells in Neurotropic Virus Infections
Malgorzata Ciurkiewicz, Vanessa Herder, Andreas Beineke
International Journal of Molecular Sciences.2020; 21(5): 1705. CrossRef - Characterization of the Th17 profile immune response in cases of human rabies transmitted by dogs and its interference in the disease pathogenesis.
L.B. Santos, F. Guedes, S.M. Achkar, M.I.S. Duarte, I.S.S. Katz, S.R. Silva, E.R. Fernandes
Journal of Neuroimmunology.2020; 344: 577263. CrossRef - Quantitative proteomics leads to identify dog brain proteins involved in rabies virus infection: implication in understanding viral pathophysiology
Suchismita Behera, Rajesh Raghunath Pharande, R. Rajendra Reddy, Sharmila B. Majee, Sandeepan Mukherjee, Amol Ratnakar Suryawanshi
Journal of Proteins and Proteomics.2020; 11(4): 241. CrossRef - Feral dog bite causing paralytic rabies: Difficult diagnosis and failure of prevention
Hussein Algahtani, Bader Shirah, Emna Chtourou, Osama Abuhawi, Nawal Abdelghaffar, Mohammad Alshehri
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