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Alireza Japoni-Nejad 2 Articles
Characterization of Plasmid-Mediated AmpC and Carbapenemases among Iranain Nosocomial Isolates of Klebsiella pneumoniae Using Phenotyping and Genotyping Methods
Alireza Japoni-Nejad, Ehsanollah Ghaznavi-Rad, Alex van Belkum
Osong Public Health Res Perspect. 2014;5(6):333-338.   Published online December 31, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.09.003
  • 1,942 View
  • 25 Download
  • 11 Citations
AbstractAbstract PDF
Objectives
Plasmid-mediated AmpC β-lactamases (PMABLs) and carbapenemases are emerging groups of antimicrobial-resistance determinants. The aims of the study were to evaluate the occurrence of PMABLs and carbapenemases in clinical isolates of Klebsiella pneumoniae and compare the test performance of various phenotypic methods for detection of these enzymes in Iran.
Methods
A total of 100 K. pneumoniae isolates were collected from clinical specimens obtained in Valiasr Hospital. AmpC production in all isolates was determined using the AmpC disk test, the cephamycin Hodge test, the AmpC Etest, and the boronic acid combined-disk test. In addition, carbapenemase production was determined using the modified Hodge test, the EDTA disk synergy test, and the boronic acid combined-disk test. The performances of various phenotypic methods were evaluated by the comparison of their results with polymerase chain reaction (PCR) method as the gold standard.
Results
Of the 100 isolates, 19 (19%) were demonstrated to harbor the PMABL-resistance gene by the multiplex PCR method. The PCR result indicated the presence of carbapenemase genes in 12 isolates. The performance of various phenotypic tests carried out for detection of carbapenemase-producing isolates varied widely, ranging in sensitivity from 30% to 100% and in specificity from 90.8% to 100%.
Conclusion
This is the first report of MOX-type AmpC β-lactamase and blaGES in K. pneumoniae in Iran. A comparison of the phenotypic methods showed that a combination of cefoxitin plus boronic acid is optimal for detecting plasmid-mediated AmpC enzymes in K. pneumoniae, whereas the implementation of molecular methods is often complex, requires specially trained personnel, and is associated with higher costs.

Citations

Citations to this article as recorded by  
  • Detection of ESBL and AmpC producing Klebsiella pneumoniae ST11 and ST147 from urinary tract infections in Iran
    Shaghayegh Shahkolahi, Pegah Shakibnia, Shahla Shahbazi, Samira Sabzi, Farzad Badmasti, Mohammad Reza Asadi Karam, Mehri Habibi
    Acta Microbiologica et Immunologica Hungarica.2022;[Epub]     CrossRef
  • Characterization of Antimicrobial Resistance Patterns of Klebsiella pneumoniae Isolates Obtained from Wound Infections
    Roya Ghanavati, Hossein Kazemian, Parisa Asadollahi , Hamid Heidari, Gholamreza Irajian, Fatemeh Navab-Moghadam, Shabnam Razavi
    Infectious Disorders - Drug Targets .2021; 21(1): 119.     CrossRef
  • An investigation of extended-spectrum β-lactamases (ESBLs) in Klebsiella isolated from foodborne outbreaks in Iran
    Farnaz Hajikarim, Mohammad Mehdi Soltan Dallal, Mohammad Reza Pourmand, Milad Abdi
    Gene Reports.2020; 19: 100632.     CrossRef
  • Prevalence and Mechanisms of Carbapenem Resistance in Klebsiella pneumoniae and Escherichia coli: A Systematic Review and Meta-Analysis of Cross-Sectional Studies from Iran
    Mohammad Javad Nasiri, Mehdi Mirsaeidi, Seyyed Mohammad Javad Mousavi, Mania Arshadi, Fatemeh Fardsanei, Behnaz Deihim, Sara Davoudabadi, Samin Zamani, Bahareh Hajikhani, Hossein Goudarzi, Mehdi Goudarzi, Zahra Sadat Seghatoleslami, Hossein Dabiri, Payam
    Microbial Drug Resistance.2020; 26(12): 1491.     CrossRef
  • Characterization of β-lactam resistance in K. pneumoniae associated with ready-to-eat processed meat in Egypt
    Shaymaa H. Abdel-Rhman, Grzegorz Woźniakowski
    PLOS ONE.2020; 15(9): e0238747.     CrossRef
  • Multiplex PCR to detect pAmpC β-lactamases among enterobacteriaceae at a tertiary care laboratory in Mumbai, India
    Mubin Kazi, Kanchan Ajbani, Jeffrey A. Tornheim, Anjali Shetty, Camilla Rodrigues
    Microbiology.2019; 165(2): 246.     CrossRef
  • AmpC β lactamases in Urinary Klebsiella pneumoniae Isolates: First Report of ACC Type AmpC β-lactamase Resistance in Iran
    Maryam Ghane, Laleh Babaeekhou, Mahdi Jafar Shanjani
    Journal of Advances in Medical and Biomedical Rese.2019; 27(123): 23.     CrossRef
  • The Molecular and Clinical Epidemiology of Extended-Spectrum Cephalosporin– and Carbapenem-Resistant Enterobacteriaceae at 4 US Pediatric Hospitals
    Danielle M Zerr, Scott J Weissman, Chuan Zhou, Matthew P Kronman, Amanda L Adler, Jessica E Berry, Jaipreet Rayar, Jeff Myers, Wren L Haaland, Carey-Ann D Burnham, Alexis Elward, Jason Newland, Rangaraj Selvarangan, Kaede V Sullivan, Theoklis Zaoutis, Xua
    Journal of the Pediatric Infectious Diseases Socie.2017; 6(4): 366.     CrossRef
  • Previous Antibiotic Exposure Increases Risk of Infection with Extended-Spectrum-β-Lactamase- and AmpC-Producing Escherichia coli and Klebsiella pneumoniae in Pediatric Patients
    Danielle M. Zerr, Arianna Miles-Jay, Matthew P. Kronman, Chuan Zhou, Amanda L. Adler, Wren Haaland, Scott J. Weissman, Alexis Elward, Jason G. Newland, Theoklis Zaoutis, Xuan Qin
    Antimicrobial Agents and Chemotherapy.2016; 60(7): 4237.     CrossRef
  • Molecular characterization of extended-spectrum β-lactamase, plasmid-mediated AmpC cephalosporinase and carbapenemase genes among Enterobacteriaceae isolates in five medical centres of East and West Azerbaijan, Iran
    Mohammad Reza Sadeghi, Reza Ghotaslou, Mohammad Taghi Akhi, Mohammad Asgharzadeh, Alka Hasani
    Journal of Medical Microbiology .2016; 65(11): 1322.     CrossRef
  • High Prevalence of AmpC β-Lactamases in Clinical Isolates of Escherichia coli in Ilam, Iran
    Abbas Maleki, Afra Khosravi, Sobhan Ghafourian, Iraj Pakzad, Shiva Hosseini, Rashid Ramazanzadeh, Nourkhoda Sadeghifard
    Osong Public Health and Research Perspectives.2015; 6(3): 201.     CrossRef
High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran
Mohammad Kargar, Zahra Mohammadalipour, Abbas Doosti, Shahrokh Lorzadeh, Alireza Japoni-Nejad
Osong Public Health Res Perspect. 2014;5(4):193-198.   Published online August 31, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.06.003
  • 2,200 View
  • 22 Download
  • 34 Citations
AbstractAbstract PDF
Objectives
Horizontal transfer of integrons is one of the important factors that can contribute to the occurrence of multidrug-resistant (MDR) bacteria. This study aimed to determine the prevalence of integrons among MDR Escherichia coli strains isolated from stool specimens and investigate the associations between the existence of integrons and MDR properties in the southwest of Iran.
Methods
There were 164 E. coli strains isolated from January 2012 to June 2012. Fecal specimens identified as E. coli by the conventional methods. Subsequently the antibiotic resistance was assessed using Clinical and Laboratory Standard Institute criteria. The presence of class 1–3 integrons and embedded gene cassettes was verified using specific primers by multiplex polymerase chain reaction assay.
Results
Among a total of 164 studied samples, 69 (42.07%) isolates were multidrug resistant. Class 1 and class 2 integrons were present in 78.26% and 76.81% MDR isolates, respectively. For the first time in Iran, class 3 integron was observed in 26.09% MDR isolates. Significant correlations were identified between: class 1 integron and resistance to amikacin, gentamicin, chloramphenicol, ampicillin, tetracycline, nalidixic acid, and co-trimoxazole; class 2 integron and resistance to aminoglycosides, co-trimoxazole, cefalexin, ampicillin, and chloramphenicol; and class 3 integron and resistance to gentamicin, kanamycin, and streptomycin.
Conclusion
Our results indicate that integrons are common among MDR isolates and they can be used as a marker for the identification of MDR isolates. Therefore, due to the possibility of a widespread outbreak of MDR isolates, molecular surveillance and sequencing of the integrons in other parts of the country is recommended.

Citations

Citations to this article as recorded by  
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    Science of The Total Environment.2022; 820: 153176.     CrossRef
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    Ahmad Khorshidi, NadiaMohammad Zadeh, Azad Khaledi, GholamAbbas Moosavi, Ali Shakerimoghaddam, Azade Matinpur
    International Archives of Health Sciences.2022; 9(1): 47.     CrossRef
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    Parastoo Ashouri, Jafar Mohammadshahi, Vajihe Sadat Nikbin, Hadi Peeridogaheh, Behnam Mohammadi-Ghalehbin, Siheila Refahi, Amir Teimourpour, Majid Esmaelizad, Hafez Mirzaneghad, Roghayeh Teimourpour
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